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  4. Recombinant Human Two pore calcium channel protein 2 (TPCN2), partial

Recombinant Human Two pore calcium channel protein 2 (TPCN2), partial

  • 中文名称：
    人TPCN2重组蛋白
  • 货号：
    CSB-YP836781HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人TPCN2重组蛋白
  • 货号：
    CSB-EP836781HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人TPCN2重组蛋白
  • 货号：
    CSB-EP836781HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人TPCN2重组蛋白
  • 货号：
    CSB-BP836781HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人TPCN2重组蛋白
  • 货号：
    CSB-MP836781HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TPCN2
  • Uniprot No.：
  • 别名：
    SHEP10; TPC2; TPC2_HUMAN; tpcn2; Two pore calcium channel protein 2; two pore segment channel 2; Two-pore channel 2; Voltage dependent calcium channel protein TPC2; Voltage-dependent calcium channel protein TPC2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Intracellular channel initially characterized as a non-selective Ca(2+)-permeable channel activated by NAADP (nicotinic acid adenine dinucleotide phosphate), it is also a highly-selective Na(+) channel activated directly by PI(3,5)P2 (phosphatidylinositol 3,5-bisphosphate). Localizes to the lysosomal and late endosome membranes where it regulates organellar membrane excitability, membrane trafficking, and pH homeostasis. Is associated with a plethora of physiological processes, including mTOR-dependent nutrient sensing, skin pigmentation and autophagy (Probable). Ion selectivity is not fixed but rather agonist-dependent and under defined ionic conditions, can be readily activated by both NAADP and PI(3,5)P2. As calcium channel, it increases the pH in the lysosomal lumen, as sodium channel, it promotes lysosomal exocytosis. Plays a crucial role in endolysosomal trafficking in the endolysosomal degradation pathway and is potentially involved in the homeostatic control of many macromolecules and cell metabolites. Unlike the voltage-dependent TPCN1, TPCN2 is voltage independent and can be activated solely by PI(3,5)P2 binding. In contrast, PI(4,5)P2, PI(3,4)P2, PI(3)P and PI(5)P have no obvious effect on channel activation.; (Microbial infection) During Ebola virus (EBOV) infection, controls the movement of endosomes containing virus particles and is required by EBOV to escape from the endosomal network into the cell cytoplasm.; (Microbial infection) Required for cell entry of coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus EMC (HCoV-EMC), by endocytosis.
  • 基因功能参考文献：
    1. PI(3,5)P2-binding site in hTPC2 identified at positively charged amino acids (K203, K204, and K207) in the linker between transmembrane helices S4 and S5 and by S322 in the cytosolic extension of S6 and protein-lipid interface upon mutations of residues within the lipid-binding pocket had neither an effect on the binding behavior nor on the channel's lipid sensitivity. PMID: 29705952
    2. TPC2 polymorphisms are associated with a hair pigmentation. PMID: 28923947
    3. Data (including data from studies using tissue from knockout mice) suggest that beta-adrenergic stimulation of pancreas leads to glucagon secretion by hierarchy of calcium signaling in glucagon-secreting cell; such signaling is initiated by cAMP-induced TPC2-dependent calcium release from acidic stores and is further amplified by calcium release from endoplasmic reticulum. PMID: 29563152
    4. the divergent pore regions from human TPC2, a two-domain channel that holds a key intermediate position in the evolution of voltage-gated ion channels, were characterized. PMID: 27941820
    5. The ion selectivity of Arabidopsis thaliana TPC1 was compared with the selectivity of human TPC2. HsTPC2 was confirmed as a Na(+)-selective channel activated by phosphatidylinositol 3,5-bisphosphate. The ion permeability ratios of HsTPC2 and its mutants were calculated. PMID: 28096396
    6. Results demonstrated the unique bell-shaped regulation of hTPC2 channel activity by [NAADP] and that channel activity is modulated by PKA phosphorylation at position S666. PMID: 26838264
    7. TPC2 regulates pigmentation through two fundamental determinants of melanosome function: pH and size. PMID: 27140606
    8. rs1551305 single nucleotide polymorphism is associated with type 2 diabetes risk. PMID: 26918892
    9. Results show that PDGs use previously unknown mechanisms of membrane dynamics and content exchange that are regulated by TPC2. PMID: 26202466
    10. Here, using live cell imaging, the s obtained evidence that in contrast to the new model, ebolavirus enters cells through endolysosomes that contain both NPC1 and TPC2. PMID: 26468524
    11. SNPs within Tpcn2 are associated with fasting insulin in humans. PMID: 25236446
    12. These results demonstrate that a VEGFR2/NAADP/TPC2/Ca(2+) signaling pathway is critical for VEGF-induced angiogenesis PMID: 25331892
    13. Notably, NAADP-mediated Ca(2+) release in intact cells is regulated by Mg(2+), PI(3,5)P2, and P38/JNK kinases, thus paralleling regulation of TPC2 currents. PMID: 24502975
    14. These results provide strong evidence for modulation of store-operated Ca(2+) entry by TPC2 involving de novo association between TPC2 and STIM1, as well as Orai1, in human cells. PMID: 23077736
    15. TPC2 is a novel NAADP-sensitive Ca2+ release channel, operating as a dual sensor of luminal pH and Ca2+. PMID: 20720007
    16. analysis of two-pore channel 2 (TPCN2)-mediated Ca2+ currents in isolated lysosomes PMID: 20495006
    17. Two coding variants in TPCN2 are associated with hair color, and a variant at the ASIP locus shows strong association with skin sensitivity to sun, freckling and red hair. PMID: 18488028

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  • 亚细胞定位：
    Late endosome membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Calcium channel alpha-1 subunit (TC 1.A.1.11) family, Two pore calcium channel subfamily
  • 组织特异性：
    Widely expressed. Expressed at high level in liver and kidney.
  • 数据库链接：

    HGNC: 20820

    OMIM: 612163

    KEGG: hsa:219931

    STRING: 9606.ENSP00000294309

    UniGene: Hs.131851