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  4. Recombinant Human Tubulin beta-8 chain (TUBB8)

Recombinant Human Tubulin beta-8 chain (TUBB8)

  • 货号：
    CSB-YP676987HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP676987HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP676987HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP676987HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP676987HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    bA631M21.2; Class VIII beta tubulin; HSA10p15 beta tubulin 4Q; RP11-631M21.2; TBB8_HUMAN; TUBB8; Tubulin beta 8; Tubulin beta 8 chain; Tubulin beta 8 class VIII; Tubulin beta-8 chain
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-444
  • 氨基酸序列
    MREIVLTQIG QCGNQIGAKF WEVISDEHAI DSAGTYHGDS HLQLERINVY YNEASGGRYV PRAVLVDLEP GTMDSVRSGP FGQVFRPDNF IFGQCGAGNN WAKGHYTEGA ELMESVMDVV RKEAESCDCL QGFQLTHSLG GGTGSGMGTL LLSKIREEYP DRIINTFSIL PSPKVSDTVV EPYNATLSVH QLIENADETF CIDNEALYDI CSKTLKLPTP TYGDLNHLVS ATMSGVTTCL RFPGQLNADL RKLAVNMVPF PRLHFFMPGF APLTSRGSQQ YRALTVAELT QQMFDAKNMM AACDPRHGRY LTAAAIFRGR MPMREVDEQM FNIQDKNSSY FADWLPNNVK TAVCDIPPRG LKMSATFIGN NTAIQELFKR VSEQFTAMFR RKAFLHWYTG EGMDEMEFTE AESNMNDLVS EYQQYQDATA EEEEDEEYAE EEVA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB8 has a key role in meiotic spindle assembly and oocyte maturation.
  • 基因功能参考文献：
    1. This TUBB8 variant in one infertile individual was absent in the 100 fertile females and was predicted to be highly damaging to the function of the TUBB8 protein by SIFT and PolyPhen-2. PMID: 29671363
    2. A homozygous mutation of TUBB8 associated with complete cleavage failure in fertilized eggs. PMID: 29704226
    3. TUBB8 mutations occur in a high proportion of infertile women with oocyte maturation arrest. PMID: 28652098
    4. The results not only emphasize the important role of TUBB8 in oocyte maturation, fertilization and early embryonic development but they also provide a basis for determining the genetic variations in TUBB8 as a potential additional criterion for evaluating the quality of patients' functional PB1 oocytes. PMID: 27989988
    5. 9 novel TUBB8 mutations and phenotypes in patients with oocyte maturation arrest were reported. PMID: 27273344
    6. TUBB8 mutations have dominant-negative effects that disrupt microtubule behavior and oocyte meiotic spindle assembly and maturation, causing female infertility. PMID: 26789871

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  • 相关疾病：
    Oocyte maturation defect 2 (OOMD2)
  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle.
  • 蛋白家族：
    Tubulin family
  • 组织特异性：
    Expressed at a high level in oocytes, at different stages of development.
  • 数据库链接：

    HGNC: 20773

    OMIM: 616768

    KEGG: hsa:347688

    STRING: 9606.ENSP00000328808

    UniGene: Hs.532659