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  4. Recombinant Human Trypsin-1 (PRSS1)

Recombinant Human Trypsin-1 (PRSS1)

  • 货号：
    CSB-YP018811HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018811HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018811HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018811HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PRSS1
  • Uniprot No.：
  • 别名：
    Alpha-trypsin chain 2; Beta-trypsin; Cationic trypsinogen; Digestive zymogen; Nonfunctional trypsin 1; Prss1; Serine protease 1; TCR V beta 4.1; TRP1; TRY1; TRY1_HUMAN; TRY4; TRYP1; Trypsin I; Trypsinogen 1; Trypsinogen A
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    24-247
  • 氨基酸序列
    IVGGYNC EENSVPYQVS LNSGYHFCGG SLINEQWVVS AGHCYKSRIQ VRLGEHNIEV LEGNEQFINA AKIIRHPQYD RKTLNNDIML IKLSSRAVIN ARVSTISLPT APPATGTKCL ISGWGNTASS GADYPDELQC LDAPVLSQAK CEASYPGKIT SNMFCVGFLE GGKDSCQGDS GGPVVCNGQL QGVVSWGDGC AQKNKPGVYT KVYNYVKWIK NTIAANS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates.
  • 基因功能参考文献：
    1. TF-FVIIa/trypsin-mediated PAR2 activation leads to enhanced MMP-2 expression in human breast cancer cells contributing to tumor progression. PMID: 29870887
    2. report here for the first time a family from India that is positive for R122H mutation in the PRSS1 gene PMID: 29476405
    3. Studied mutations in cationic trypsinogen (PRSS1) and serine protease inhibitor Kazal type 1 (SPINK1) and their association with alcoholic chronic pancreatitis (ACP) and idiopathic chronic pancreatitis. PMID: 29641165
    4. Data indicate association between polymorphism rs10273639 of the PRSS1-PRSS2 locus and pancreatitis development. PMID: 27846138
    5. SNP in the PRSS1-PRSS2-rs10273639 is associated with Chronic pancreatitis. PMID: 28913878
    6. Gene mutations were present in PRSS1 in 26 patients with acute recurrent and chronic pancreatitis, SPINK1 in 23, CTRC in 3, and CPA1 in 5. In the 31 patients with mutations in SPINK1, CTRC, or CPA1, 16 (51.6%) had homozygous or heterozygous mutations with other mutations. PMID: 27409067
    7. Results identified 3 new rare variants in the proximal promoter region of PRSS1 which along the known c.-30_-28delTCC do not seem to be a risk factors for chronic pancreatitis. PMID: 27432637
    8. Early-onset pancreatitis is associated strongly with PRSS1 or CTRC mutations and family history of pancreatitis. PMID: 28502372
    9. PRSS1 variant p.L104P exhibits a variety of phenotypic changes that can increase risk for chronic pancreatitis. Mutation-induced misfolding and associated ER stress are the dominant effects that support a direct pathogenic role in chronic pancreatitis. PMID: 26822915
    10. Mutations in CFTR, SPINK1 or PRSS1 are present in one third of pediatric acute recurrent (ARP) or chronic (CP) pancreatitis with no other cause. PMID: 26692446
    11. Three types of pathogenic PRSS1 variants was identified in 11 patients, including p.N29I (n=1), p.R122H (n=1), and p.G208A (n=9). PMID: 27578509
    12. Mutations were found at the promoter and exon 3 of the PRSS1 in patients with pancreatic cancer. That is, five patients had c.410 C > T mutation causing p.Thr 137 Met, and three patients had c. -338 T >G mutation at the promoter of the PRSS1. In patients with PRSS1 mutations, serum trypsin was significantly higher than that in normal controls PMID: 26546433
    13. These findings suggest that IL-1beta plays a key role in trypsin upregulation and has a pathological role in multiple organ failure in influenza virus infection. PMID: 27714503
    14. Human anionic trypsinogen is controlled by CTRC in a manner that individual natural mutations are unlikely to increase stability enough to promote intra-pancreatic activation. PMID: 27129265
    15. Association of PRSS1 polymorphism with idiopathic recurrent acute pancreatitis and idiopathic chronic pancreatitis. PMID: 26110235
    16. Letter/Case Report: high penetrance of the PRSS1 A16V mutation caused by SPINK1 N34S and CFTR TG11-5T co-mutations associated with pancreatitis. PMID: 26658045
    17. We did not find PRSS1 mutation in any patient. PMID: 25981744
    18. In Israel, pediatric as well as adult recurrent acute pancreatitis and chronic pancreatitis are often associated with PRSS1, serine protease inhibitor Kazal type 1, chymotrypsin C, or Cystic Fibrosis Transmembrane Conductance Regulator mutations. PMID: 25383785
    19. This study shows that PRSS1, SPINK1 and CFTR mutations do not play a role in alcoholic and idiopathic chronic pancreatitis patients. PMID: 25835118
    20. N29T mutation associated with pancreatitis PMID: 26376395
    21. Recombination events between members of the trypsinogen family can generate high-risk PRSS1 alleles. PMID: 25546417
    22. the observations demonstrate that the tyrosyl sulfate in human trypsins interacts with the P2' position of the substrate-like inhibitor and this modification increases P2' selectivity towards basic side chains. PMID: 25010489
    23. PRSS1 single nucleotide polymorphism is associated with alcoholic pancreatitis. PMID: 25253127
    24. PRSS1 variants are associated with less disease relapse in autoimmune pancreatitis. PMID: 24909264
    25. Two novel PRSS1 mutations were identified in Mexican patients with early onset idiopathic recurrent acute pancreatitis. PMID: 25206283
    26. The -409 T/T genotype of PRSS1 gene was revealed to be a marker for predicting neotal sepsis, along with serum trypsin levels. PMID: 24777884
    27. None of the selected 121 pancreatic cancer samples showed a pancreatitis-predisposing mutation in the PRSS1 gene. PMID: 25003218
    28. expression of WT or mutant human PRSS1 in murine acinar cells induces apoptosis and is sufficient to promote spontaneous pancreatitis. PMID: 24722290
    29. Using the optimal cutoffs, the sensitivity of the IRT/PAP strategy would not be inferior to that of IRT/DNA if identification of mild forms is not required. PMID: 24513262
    30. PRSS1: IVS 2 +56_60 del CCCAG is a noval mutant which may contribute to AIP pathogenesis. PMID: 24236450
    31. The p.G208A variant of PRSS1, previously only found in Asian patients, was found in 3 generations of a European family, one of whom had chronic pancreatitis. PMID: 24413785
    32. the p.N43S mutation in the SPINK1 gene, accelerated the onset of HP in the presence of the p.R122H mutation in the CT gene. PMID: 24134754
    33. Rare causative PRSS1 mutations account for 9.1% of the idiopathic chronic pancreatitis study group in 253 young French patients. PMID: 23951356
    34. The trypsinogen to trypsin conformational switch modulates cleavage susceptibility of nick sites by altering both the thermodynamics and kinetics of cleavage to protect human cationic trypsin from premature degradation. PMID: 24403079
    35. PRSS1 hereditary pancreatitis is characterized by progressive lipomatous atrophy of the pancreas. PMID: 24525505
    36. No association of PRSS1 with pancreatic cancer is found. PMID: 23751316
    37. D21A polymorphism in the PRSS1 gene seems to be a risk factor for hereditary chronic pancreatitis. PMID: 23864476
    38. PRSS1 gene was significantly associated with total pancreatitis disease, both totally and separately PMID: 23801884
    39. For pediatric ulcerative colitis patients who require surgery, the immunopositivity of Tryp-1 at diagnosis is lower when compared to that of patients with a more benign disease course. PMID: 23745029
    40. Autoimmune pancreatitis may be related to PRSS1 gene mutations. PMID: 23745036
    41. Trypsin-1 and trypsin-2 appear to have a function in the degradation of vitreous type II collagen. PMID: 23882137
    42. A kindred from Venezuela, South America with the PRSS1 R122H variant. PMID: 23474566
    43. Even in the absence of chymotrypsin C, trypsinogen activation peptide mutants autoactivated at markedly increased rates. PMID: 23601753
    44. Case Report: suggest involvement of PRSS1 mutations in familial solid pseudopapillary pancreatic tumors. PMID: 22722260
    45. Frequencies of CFTR variants p.R75Q, p.I148T, 5T-allele and p.E528E were comparable in patients and controls. We identified 103 CFTR variants, which represents a 2.7-fold risk increase (p<0.0001). PMID: 22427236
    46. Two associations with alcoholic pancreatitis at genome-wide significance were identified and replicated at PRSS1-PRSS2 and X-linked CLDN2 through a two-stage genome-wide study. PMID: 23143602
    47. Data show that among the 2 mutations in the PRSS1 gene, only the N29I mutation was found in 1 (1.7%) of 58 patients with AP, in the heterozygous state. PMID: 22699143
    48. hereditary pancreatitis is caused by CTRC-dependent dysregulation of cationic trypsinogen autoactivation, which results in elevated trypsin levels in the pancreas. PMID: 22539344
    49. All of the heterozygote patients with a combination of CFTR and SPINK1 or PRSS1 mutations had chronic pancreatitis. PMID: 22094894
    50. Mutations of PRSS1 gene may be an important factor of pancreatic cancer. PMID: 22088471

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  • 相关疾病：
    Pancreatitis, hereditary (PCTT)
  • 亚细胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Peptidase S1 family
  • 数据库链接：

    HGNC: 9475

    OMIM: 167800

    KEGG: hsa:5644

    STRING: 9606.ENSP00000308720

    UniGene: Hs.382212