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  4. Recombinant Human Tribbles homolog 3 (TRIB3)

Recombinant Human Tribbles homolog 3 (TRIB3)

  • 中文名称：
    人TRIB3重组蛋白
  • 货号：
    CSB-YP847220HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人TRIB3重组蛋白
  • 货号：
    CSB-EP847220HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人TRIB3重组蛋白
  • 货号：
    CSB-EP847220HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人TRIB3重组蛋白
  • 货号：
    CSB-BP847220HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人TRIB3重组蛋白
  • 货号：
    CSB-MP847220HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    C20orf97; Neuronal cell death inducible putative kinase ; Neuronal cell death-inducible putative kinase; NIPK; p65 interacting inhibitor of NF-kappaB; p65-interacting inhibitor of NF-kappa-B; SINK; SKIP 3; SKIP3; TRB 3; TRB-3; TRB3; TRIB 3; Trib3; TRIB3_HUMAN; Tribbles homolog 3; Tribbles pseudokinase 3; Tribbles3
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-358
  • 氨基酸序列
    MRATPLAAPA GSLSRKKRLE LDDNLDTERP VQKRARSGPQ PRLPPCLLPL SPPTAPDRAT AVATASRLGP YVLLEPEEGG RAYQALHCPT GTEYTCKVYP VQEALAVLEP YARLPPHKHV ARPTEVLAGT QLLYAFFTRT HGDMHSLVRS RHRIPEPEAA VLFRQMATAL AHCHQHGLVL RDLKLCRFVF ADRERKKLVL ENLEDSCVLT GPDDSLWDKH ACPAYVGPEI LSSRASYSGK AADVWSLGVA LFTMLAGHYP FQDSEPVLLF GKIRRGAYAL PAGLSAPARC LVRCLLRREP AERLTATGIL LHPWLRQDPM PLAPTRSHLW EAAQVVPDGL GLDEAREEEG DREVVLYG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Inactive protein kinase which acts as a regulator of the integrated stress response (ISR), a process for adaptation to various stress. Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Acts as a negative feedback regulator of the ATF4-dependent transcription during the ISR: while TRIB3 expression is promoted by ATF4, TRIB3 protein interacts with ATF4 and inhibits ATF4 transcription activity. Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. Can inhibit APOBEC3A editing of nuclear DNA.
  • 基因功能参考文献：
    1. A link between TRIB3 and the stereotypical pattern of gray matter loss in Alzheimer's disease. PMID: 29511103
    2. the R141 site of TRIB3 dictates the strength of Akt binding but does not affect other Trbl-dependent developmental processes, suggesting a specificity that could serve as a drug target for metabolic diseases. PMID: 29025897
    3. TRB3 is overexpressed in lesions of patients with psoriasis. PMID: 28790132
    4. tribbles pseudokinase 3 (TRB3) and sestrin 2 may contribute to the development of obesity and its complications and can be considered interesting therapeutic target for the treatment of obesity PMID: 28639842
    5. TRIB3, a single-domain protein with impaired kinase catalytic activity, has been described to perform a complex of roles, many of which are likely to be cell type-specific. (Review) PMID: 28393700
    6. High TRB3 expression is associated with basal-like breast cancer. PMID: 27593927
    7. TRIB3 promotes acute promyelocytic leukemia progression through stabilization of the oncoprotein PML-RARalpha and inhibition of p53-mediated senescence. PMID: 28486108
    8. Downregulation of TRIB3 is associated with lung cancer. PMID: 28184934
    9. This study aims to investigate whether the Tribbles 3 Q84R polymorphism has profound effects on serum semaphorin 3E and what effect semaphorin 3E exerts on carotid atherosclerosis. PMID: 28249916
    10. overexpression of TRIB3 is associated with tumor angiogenesis and a poor prognosis in patients with gastric cancer (GC) . Our findings indicate that TRIB3 is a promising target for antiangiogenic therapy in GC. PMID: 27573078
    11. Our findings suggest that good glucose and blood pressure control exhibited greater benefits on vascular outcomes in patients with TRIB3 (rs2295490) G allele. PMID: 27793583
    12. In this exploratory analysis, IRS1, ENNP1 and TRIB3, known to be associated with type 2 diabetes and harboring genes playing a prominent role in mediating insulin signaling, may modulate a number of cardiometabolic phenotypes in patients of Italian ancestry with newly-diagnosed type 2 diabetes. PMID: 26868433
    13. TRIB3 acts as a crucial 'stress adjusting switch' that links homeostasis, metabolic disease and cancer; and is being actively investigated as a disease biomarker and therapeutic target PMID: 26855171
    14. TRB3 is upregulated in macrophages upon treatment with ox-LDL. TRB3 promotes lipid accumulation and suppresses cytokine expression, suggesting inflammation and foam cell formation can be reciprocally regulated. PMID: 26584255
    15. Studies suggest that pseudo-kinase family of tribbles (TRIB) proteins TRIB1, TRIB2 and TRIB3 play roles in pathogenesis of rheumatoid arthritis (RA) and osteoarthritis. PMID: 26517922
    16. Studies suggest that pseudo-kinase family of tribbles (TRIB) proteins TRIB1, TRIB2 and TRIB3 were involved in the pathogenesis of inflammation. PMID: 26517925
    17. Studies indicate that small molecules can reveal rate-limiting signalling outputs and functions of pseudo-kinase family of tribbles (TRIB) proteins TRIB1, TRIB2 and TRIB3 in cells and intact organisms, serving as guides for the development of new drugs. PMID: 26517930
    18. Studies suggest that the tribbles homolog 3 (Drosophila) protein (TRIB3) Q84R missense polymorphis contributes to atherosclerosis. PMID: 26517932
    19. Studies show a remarkable reduction in tribbles 2 protein (Trib2) expression during oocyte maturation whereas tribbles 1 protein (Trib1) and tribbles 3 protein (Trib3) expression was significantly increased during this process. PMID: 26517934
    20. Studies show that tribbles pseudokinase 3 (TRIB3) plays a crucial role in the anti-cancer activity of cannabinoids and that TRIB3 genetic inactivation enhances cancer generation and progression. PMID: 26517935
    21. TRB3 is an important mediator of palmitate-induced apoptosis in human liver cells, suggesting that it may also be involved in the molecular mechanism underlying PNALD. PMID: 24685558
    22. TRB3 is a novel profibrotic mediator in SSc. TGF-beta induces TRB3, which in turn activates canonical TGF-beta/Smad signaling and stimulates the release of collagen, creating a positive feedback loop that may contribute to aberrant TGF-beta signalling in SSc. PMID: 25603829
    23. Suggest a role of TRIB3 low-frequency variants on coronary artery disease risk among Italian patients with T2D. PMID: 26253791
    24. The results of the present study suggested that TRIB3 was associated with high glucose-induced HUVECs apoptosis, which was attenuated following transfection with TRIB3 siRNA. PMID: 25845379
    25. These results implicate TRIB3 and IGFBP2, both of which are known to be overexpressed in several types of cancers, as pro-survival modulators of cell viability in nutrient-deficient microenvironments. PMID: 26094770
    26. Trib3 interferes with the Parkinson's disease-associated prosurvival protein Parkin to mediate death PMID: 26224857
    27. These observations support the notion that loss of TRIB3 is associated with a more aggressive phenotype in various types of tumors by enhancing the activity of the mTORC2/AKT/FOXO axis. PMID: 25168244
    28. smoking and the TRIB3 G-allele is associated with an increased risk of diabetic nephropathy, which supports the hypothesis that oxidative stress contributes to the development of diabetic nephropathy PMID: 25447894
    29. The correlation between TRB3 and Notch 1 expression revealed that Notch 1 was downregulated by the knockdown of TRB3 in the lung adenocarcinoma cell lines. PMID: 23632994
    30. Tribbles 3 mediates endoplasmic reticulum stress-induced insulin resistance in skeletal muscle. PMID: 23695665
    31. Results demonstrate that both TRB3 and p21(Cip1) are critical molecules in the homocysteine signaling cascade. PMID: 23349842
    32. We discuss the role of Tribs as central signaling mediators in different subtypes of acute leukemia and propose that inhibition of dysregulated Trib signaling may be therapeutically beneficial. PMID: 23550039
    33. TRIB3 protein is a stable protein which levels are predominantly regulated by translational control of TRIB3 mRNA transcript in breast cancer PMID: 23185332
    34. Data indicate a dominant role for TRB3 and MAPK-ERK/TGFbeta pathways as Notch regulators in breast cancer, establishing TRB3 as a potential therapeutic target. PMID: 23319603
    35. TRB3, through its own cleavage, functions as a molecular switch between the cell survival and apoptotic pathways under stressful conditions PMID: 22912727
    36. Tribbles 3 protects nuclear DNA from cytidine deamination by APOBEC3A PMID: 22977230
    37. molecular mechanisms underlying metabolic and cardiovascular abnormalities [review] PMID: 22577090
    38. TRIB3 is independently associated with poor prognosis of breast cancer patients, possibly through its association with tumor cell hypoxia PMID: 21864376
    39. Studies indicate tribbles 1 and tribbles 3 as regulators of lipid level and disease susceptibility genes in metabolic syndrome and type 2 diabetes. PMID: 22274752
    40. TRIB3 protein is associated with a good prognosis in human breast cancer patients, possibly due to the fact that TRIB3 is involved in hypoxia tolerance. PMID: 21704407
    41. knockdown of endogenous TRB3 expression inhibited the migration and invasion of tumor cells in vitro PMID: 21896644
    42. TRB3 is a critical molecule in the homocysteine-mediated cell-cycle arrest in endothelial cells. PMID: 21935927
    43. TRB3 Q84R polymorphism is associated with obesity and especially glucose metabolism and not associated with polycystic ovary syndrome because of compositional characteristics of phenotype in Chinese PCOS women. PMID: 21492415
    44. HHcy impairs hepatic glycogen synthesis by inducing the expression of TRB3 PMID: 21435438
    45. the GCN2/eIF2alpha/ATF4 pathway is essential for the induction of the TRB3 gene transcription PMID: 21203563
    46. Helicobacter pylori infection is associated with decreased expression of TRIB3 in human gastric epithelial cell lines and tissue samples. PMID: 21220698
    47. The TRIB3 R84 variant is associated with increased carotid IMT also in Caucasians, thus replicating previous data obtained in Asians. In addition, in HUVECs, this variant is associated with unbalanced insulin signalling. PMID: 20693163
    48. Study have found evidence for a role of aberrant hepatic TRIB3 transcript levels in insulin resistance in obese humans and identified potential transcriptional pathways involved in regulation of TRIB3 gene expression in the liver. PMID: 20461355
    49. The pseudokinase tribbles homolog 3 interacts with ATF4 to negatively regulate insulin exocytosis in human and mouse beta cells. PMID: 20592469
    50. Data confirm that the TRIB3 R84 variant affects glucose homeostasis and suggest this effect is due to an alteration of the interplay between insulin sensitivity and secretion. PMID: 20393693

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, Tribbles subfamily
  • 组织特异性：
    Highest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, includ
  • 数据库链接：

    HGNC: 16228

    OMIM: 607898

    KEGG: hsa:57761

    STRING: 9606.ENSP00000217233

    UniGene: Hs.516826