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  4. Recombinant Human Translin (TSN)

Recombinant Human Translin (TSN)

  • 货号：
    CSB-YP025142HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP025142HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP025142HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP025142HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP025142HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TSN
  • Uniprot No.：
  • 别名：
    BCLF 1; BCLF1; Component 3 of promoter of RISC; RCHF1; recombination hotspot associated factor; recombination hotspot binding protein; rehf 1; REHF1; TBRBP; Translin; TRSLN; TSN; TSN_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-228
  • 氨基酸序列
    MSVSEIFVEL QGFLAAEQDI REEIRKVVQS LEQTAREILT LLQGVHQGAG FQDIPKRCLK AREHFGTVKT HLTSLKTKFP AEQYYRFHEH WRFVLQRLVF LAAFVVYLET ETLVTREAVT EILGIEPDRE KGFHLDVEDY LSGVLILASE LSRLSVNSVT AGDYSRPLHI STFINELDSG FRLLNLKNDS LRKRYDGLKY DVKKVEEVVY DLSIRGFNKE TAAACVEK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    DNA-binding protein that specifically recognizes consensus sequences at the breakpoint junctions in chromosomal translocations, mostly involving immunoglobulin (Ig)/T-cell receptor gene segments. Seems to recognize single-stranded DNA ends generated by staggered breaks occurring at recombination hot spots.; Exhibits both single-stranded and double-stranded endoribonuclease activity. May act as an activator of RNA-induced silencing complex (RISC) by facilitating endonucleolytic cleavage of the siRNA passenger strand.
  • 基因功能参考文献：
    1. Translin and Trax control telomere-associated transcript levels in human cells in a telomere-specific fashion. PMID: 27183912
    2. results indicate that UPF1 can dissociate miRNAs from their mRNA targets, making the miRNAs susceptible to Tudor-staphylococcal/micrococcal-like nuclease (TSN)-mediated miRNA decay. PMID: 28827400
    3. These findings indicate that miRNA depletion in dicer deficiency is due to the combined loss of miRNA-generating activity and catabolic function of TN/TX. PMID: 25457613
    4. this study argues against a mechanism by which translin octamers may "dissociate and reassemble" upon RNA binding and report a novel "open"-barrel structure of human translin revealing a feasible DNA/RNA entryway into the cavity. PMID: 25433126
    5. The selectivity of PLCbeta toward certain genes lies in the rate at which the RNA is hydrolyzed by C3PO. PMID: 24338081
    6. The data demonstrates that the configuration of translin and translin-RNA complexes in solution is a dimer of tetramers, which undergoes conformational transitions. PMID: 23980029
    7. Translin B3 motif, corresponding to TRAX B3 residues, contributes towards its DNA binding activity. PMID: 22427937
    8. C3PO endonuclease is an asymmetric octamer barrel consisting of six translin and two TRAX subunits which activates RISC by degrading the Ago2-nicked passenger strand of siRNA. PMID: 21552258
    9. Sedimentation equilibrium studies using fluorescein-labeled single-stranded DNA support a model in which translin forms an annular structure of eight subunits, an oblate ellipsoid that binds single-stranded DNA at chromosomal breakpoints. PMID: 11705401
    10. study of reactivity of two -SH groups and stidu of proximity of Cys-225 residues PMID: 11876655
    11. At high enough concentrations, translin binds duplex DNA by selectively loading from the free ends, which apparently results in clamping of strands at the duplex ends. PMID: 12484770
    12. The core protein of HCV can interact with translin protein. This can partly explain the molecular mechanism for hepatocellular carcinoma and lymphoma caused by HCV PMID: 12532453
    13. TB-RBP post-transcriptionally stabilizes TRAX and both proteins are needed for normal cell proliferation PMID: 14711818
    14. structure of human translin at 2.2 A resolution is reported in space group C222; translin forms a tetramer; this suggests that the multimerization of translin is flexible; the flexibility may be related to the binding to DNA/RNA PMID: 15039555
    15. Results suggest that translin might be involved in the control of recombination at d(GT)n.d(AC)n microsatellites and in telomere maintenance. PMID: 15544804
    16. Translin "chaperones" Trax and forms heteromeric complex that is DNA binding competent PMID: 15919079
    17. Results describe a circular dichroism analysis of complexes formed between human translin and the microsatellite and telomeric oligodeoxynucleotides d(GT)(12) and d(TTAGGG)(5). PMID: 16218753
    18. Human translin exhibits a stable octameric state and binds ssDNA/RNA/dsDNA targets, all of which get attenuated when GTP is added PMID: 16411762
    19. Findings implicate abnormal dendritic trafficking of BDNF mRNA in the pathophysiology of neuropsychiatric disorders linked to the translin G196A mutation. PMID: 19805324

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Translin family
  • 数据库链接：

    HGNC: 12379

    OMIM: 600575

    KEGG: hsa:7247

    STRING: 9606.ENSP00000374332

    UniGene: Hs.75066