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  4. Recombinant Human Transcriptional repressor CTCFL (CTCFL)

Recombinant Human Transcriptional repressor CTCFL (CTCFL)

  • 货号：
    CSB-YP818824HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP818824HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP818824HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP818824HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP818824HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CTCFL
  • Uniprot No.：
  • 别名：
    BORIS like protein; Brother of the regulator of imprinted sites; Cancer/testis antigen 27; CCCTC binding factor (zinc finger protein) like; CCCTC-binding factor; CT27; CTCF paralog; CTCF T; CTCF-like protein; Ctcfl; CTCFL_HUMAN; dJ579F20.2; HMG 1L1; HMGB1L1; MGC163358; MGC169105; MGC169106; Putative high mobility group protein 1 like 1; Putative high mobility group protein B1 like 1; Transcriptional repressor CTCFL; Zinc finger protein CTCF-T
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-663
  • 氨基酸序列
    MAATEISVLS EQFTKIKELE LMPEKGLKEE EKDGVCREKD HRSPSELEAE RTSGAFQDSV LEEEVELVLA PSEESEKYIL TLQTVHFTSE AVELQDMSLL SIQQQEGVQV VVQQPGPGLL WLEEGPRQSL QQCVAISIQQ ELYSPQEMEV LQFHALEENV MVASEDSKLA VSLAETTGLI KLEEEQEKNQ LLAERTKEQL FFVETMSGDE RSDEIVLTVS NSNVEEQEDQ PTAGQADAEK AKSTKNQRKT KGAKGTFHCD VCMFTSSRMS SFNRHMKTHT SEKPHLCHLC LKTFRTVTLL RNHVNTHTGT RPYKCNDCNM AFVTSGELVR HRRYKHTHEK PFKCSMCKYA SVEASKLKRH VRSHTGERPF QCCQCSYASR DTYKLKRHMR THSGEKPYEC HICHTRFTQS GTMKIHILQK HGENVPKYQC PHCATIIARK SDLRVHMRNL HAYSAAELKC RYCSAVFHER YALIQHQKTH KNEKRFKCKH CSYACKQERH MTAHIRTHTG EKPFTCLSCN KCFRQKQLLN AHFRKYHDAN FIPTVYKCSK CGKGFSRWIN LHRHSEKCGS GEAKSAASGK GRRTRKRKQT ILKEATKGQK EAAKGWKEAA NGDEAAAEEA STTKGEQFPG EMFPVACRET TARVKEEVDE GVTCEMLLNT MDK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites. Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.
  • 基因功能参考文献：
    1. aberrant expression of BORIS inhibits apoptosis, promotes proliferation, and attenuates the sensitivity of colorectal cancer cells to 5-FU treatment. PMID: 28098226
    2. These findings highlight the role of intragenic DNA methylation and DNA binding protein BORIS in cancer-specific splicing and its role in tumorigenesis. PMID: 29073069
    3. The CCCTC-binding factor plays critical roles in genome topology and function, increased risk of carcinogenicity, and potential of lung cancer-specific mediations. Chromosome reposition in lung cancer can be regulated by CCCTC binding factor PMID: 27840277
    4. These data provide information on the organization of the BORIS promoter region and gene regulation in normal and cancer cells. In addition, the study proposes that specific alleles of the BORIS-MS2 region could be used to identify the risk for lung cancer. PMID: 27416782
    5. the results indicate that the CT antigen BORIS sf6 is specifically expressed in cervical cancer stem-like cells and cancer-initiating cells PMID: 26849232
    6. BORIS is associated with the cancer stem cell-like traits of human liver cancer cells through the epigenetic regulation of OCT4. PMID: 28645561
    7. In this study, the methylation status of BORIS was tested by methylation specific polymerase chain reaction in human hepatocarcinoma (HCC) cell lines and 43 pairs of tissue specimens. Frequently demethylation of BORIS in HCC was significantly higher than that in the paired adjacent non-tumor tissues (P=0.019), and it was correlated with tumor size (P=0.025) and clinical TNM stage (P=0.035). PMID: 28764977
    8. Effectively controlling BORIS/CTCFL levels can inhibit disease establishment and hence can be considered as a potent target for cancer therapy PMID: 28104398
    9. BORIS is associated with cancer stem cell-enriched populations of several epithelial tumor cells and the different phenotypes depend on the origin of tumor cells. PMID: 26185996
    10. Data found that BORIS and CTCF expression in low-grade squamous intraepithelial lesions and invasive cervical carcinoma is higher than in normal samples. The possible utility of BORIS and CTCF as biomarkers in cervical neoplasm requires further analysis. PMID: 26125810
    11. Differential regulation of MAGE-A1 promoter activity by BORIS and Sp1, both interacting with the TATA binding protein. PMID: 25363021
    12. High BORIS transcript variants are associated with laryngeal squamous cell carcinomas. PMID: 24563233
    13. CTCFL/BORIS was amongst the top ranked genes differentially expressed between endometrioid and non-endometrioid tumors, and increasing mRNA level of CTCFL/BORIS was highly significantly associated with poor survival. PMID: 24658009
    14. The serum levels of MAGE-A and BORIS mRNA, as well as let-7b were significantly higher in patients. PMID: 24983365
    15. results provide novel insights into the determinants of NOTCH3 overexpression in cancer cells, by revealing a key role for BORIS as the main mediator of transcriptional deregulation of NOTCH3 PMID: 24984200
    16. BORIS is an RNA-binding protein that associates with polysomes. PMID: 24279897
    17. Neither the MTHFR polymorphism, nor CTCFL mutations explain a pattern of sperm hypomethylation at paternally imprinting loci PMID: 23955684
    18. The ability of BORIS to activate the androgen receptor gene indicates BORIS involvement in the growth and development of prostate tumors. PMID: 24123052
    19. The expression of BORIS isoform families in normal ovary (NO) and epithelial ovarian cancer. PMID: 23390377
    20. CTCF and BORIS suppressed breast cancer growth; findings provide further evidence that CTCF behaves as a tumor suppressor, and show BORIS has a similar growth inhibitory effect in vitro and in vivo PMID: 23553099
    21. our study has shown for the first time that BORIS is expressed in hepatocellular carcinoma (HCC) tissues, and its expression significantly correlates with poor prognosis and cd90 expression. PMID: 23237599
    22. BORIS induces demethylation of the SBSN CpG island and disruption and activation of chromatin around the SBSN transcription start site, resulting in an increase in SBSN expression. PMID: 22792300
    23. down-regulation of endogenous BORIS by specific shRNAs inhibited both RNA transcription and cell cycle progression. The results altogether suggest a role for BORIS in coordinating S phase events with mitosis. PMID: 22724006
    24. BORIS may be a useful biomarker for prognostic diagnosis of ESCC patients and a potential target for treatment including by BORIS-specific immunotherapy and molecular target therapy. PMID: 22676270
    25. This review summarizes what is known about BORIS regarding its expression, structure, and function. PMID: 22168535
    26. Transcription of the ferT gene in colon cancer cells was found to be driven by an intronic promoter residing in intron 10 of the fer gene and to be regulated by the Brother of the Regulator of Imprinted Sites (BORIS) transcription factor. PMID: 22223638
    27. a significant up-regulation of BORIS (p<0.001) and TSHZ1 transcripts (p<0.05) for JAs compared to nasal mucosa. PMID: 21874228
    28. These findings show that BORIS expression is more widespread than previously believed, and suggest a role for BORIS in nucleolar function. PMID: 21811597
    29. BORIS-MS2 alleles are transmitted through meiosis following Mendelian inheritance, which suggests that this polymorphic minisatellite could be a useful marker for paternity mapping and DNA fingerprinting. PMID: 21495859
    30. BORIS positively regulates these CTAs by binding and inducing a shift to a more open chromatin conformation with promoter demethylation PMID: 21558405
    31. Data show that the BORIS/CTCF mRNA expression ratio is closely associated with DNA hypomethylation and confers poor prognosis in EOC. PMID: 21296871
    32. The activity of CTCF in controlling Rb2/p130 gene expression is impaired by BORIS, which by binding to the Rb2/p130 gene could trigger changes in the chromatin asset established by CTCF affecting CTCF regulatory activity on Rb2/p130 transcription. PMID: 21325284
    33. Data suggest that the BORIS gene is involved in a range of functionally important aspects of both normal gametogenesis and cancer development. PMID: 21079786
    34. The short rare alleles of BORIS-MS2 could be used to identify the risk for breast cancer in young patients. PMID: 21034534
    35. BORIS (CTCFL) is not expressed in most human breast cell lines and high grade breast carcinomas PMID: 20305816
    36. Expression of the cancer-germline (CG) (or cancer-testis) antigen gene BORIS/CTCFL has been proposed to mediate activation of CG antigen genes in cancer. PMID: 20649179
    37. the C-terminal fragment of CTCFL predominantly consists of extended and unordered content. PMID: 20438700
    38. As no mutations were detected at CTCFL in the patients examined, we conclude that genetic alterations of CTCFL are not responsible for the SRS hypomethylation. PMID: 19675668
    39. Data indicate that reciprocal binding of CTCF and BORIS to the NY-ESO-1 promoter mediates epigenetic regulation of this CT gene in lung cancer cells. PMID: 16140944
    40. The lack of hypomethylation in the CFCFL promoter reinforces evidence that 'genome-wide' hypomethylation is not random. PMID: 16854382
    41. BORIS represents a new class of cancer biomarkers different from those currently used in medical practice. PMID: 17062669
    42. we detected a high frequency of BORIS expression in uterine cancers. PMID: 17363524
    43. The expression of BORIS antigen is much stronger in breast cancer than that in benign breast disease and normal breast tissues, which indicate that BORIS may be involved in the pathogenesis of the breast cancer. PMID: 17428394
    44. Activation of BORIS is associated with melanoma PMID: 17957795
    45. Alternative promoter usage generated at least five alternatively spliced BORIS mRNAs with different half-lives determined by varying 5'-UTRs. PMID: 17962299
    46. These data establish promoter DNA hypomethylation as a mechanism leading to BORIS expression in human ovarian cancer. PMID: 18095639
    47. The ability of BORIS to activate promoters of the RP and ER genes points towards possible involvement of BORIS in the establishment, progression and maintenance of breast tumours. PMID: 18195709
    48. Chromatin immunoprecipitation analysis of the BAG-1 promoter in DNMT1-overexpressing or DNMT3B-overexpressing colon cells show a permissive chromatin status assoscsiated with DNA binding of BORIS. PMID: 18413740
    49. Differential expression of the cancer/testis gene BORIS in human preimplantation development. PMID: 18467432
    50. CTCFL/BORIS is a methylation-independent DNA-binding protein that preferentially binds to the paternal H19 differentially methylated region PMID: 18632606

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    CTCF zinc-finger protein family
  • 组织特异性：
    Testis specific. Specifically expressed in primary spermatocytes.
  • 数据库链接：

    HGNC: 16234

    OMIM: 607022

    KEGG: hsa:140690

    STRING: 9606.ENSP00000243914

    UniGene: Hs.131543