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  4. Recombinant Human Transcriptional regulator Kaiso (ZBTB33)

Recombinant Human Transcriptional regulator Kaiso (ZBTB33)

  • 货号：
    CSB-YP769768HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP769768HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP769768HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP769768HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP769768HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ZBTB33
  • Uniprot No.：
  • 别名：
    Kaiso; Kaiso protein; Kaiso transcription factor; KAISO_HUMAN; Transcriptional regulator Kaiso; ZBTB 33; Zbtb33; Zinc finger and BTB domain containing 33; Zinc finger and BTB domain-containing protein 33; Zinc finger transcription factor Kaiso; ZNF 348; ZNF Kaiso; ZNF348
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-672
  • 氨基酸序列
    MESRKLISAT DIQYSGSLLN SLNEQRGHGL FCDVTVIVED RKFRAHKNIL SASSTYFHQL FSVAGQVVEL SFIRAEIFAE ILNYIYSSKI VRVRSDLLDE LIKSGQLLGV KFIAELGVPL SQVKSISGTA QDGNTEPLPP DSGDKNLVIQ KSKDEAQDNG ATIMPIITES FSLSAEDYEM KKIIVTDSDD DDDDVIFCSE ILPTKETLPS NNTVAQVQSN PGPVAISDVA PSASNNSPPL TNITPTQKLP TPVNQATLSQ TQGSEKLLVS SAPTHLTPNI ILLNQTPLST PPNVSSSLPN HMPSSINLLV QNQQTPNSAI LTGNKANEEE EEEIIDDDDD TISSSPDSAV SNTSLVPQAD TSQNTSFDGS LIQKMQIPTL LQEPLSNSLK ISDIITRNTN DPGVGSKHLM EGQKIITLDT ATEIEGLSTG CKVYANIGED TYDIVIPVKD DPDEGEARLE NEIPKTSGSE MANKRMKVKH DDHYELIVDG RVYYICIVCK RSYVCLTSLR RHFNIHSWEK KYPCRYCEKV FPLAEYRTKH EIHHTGERRY QCLACGKSFI NYQFMSSHIK SVHSQDPSGD SKLYRLHPCR SLQIRQYAYL SDRSSTIPAM KDDGIGYKVD TGKEPPVGTT TSTQNKPMTW EDIFIQQEND SIFKQNVTDG STEFEFIIPE SY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. Represses expression of MMP7 in conjunction with transcriptional corepressors CBFA2T3, CBFA2T2 and RUNX1T1.
  • 基因功能参考文献：
    1. The level of miR-4262 was found to be significantly decreased in CC tissues and cell lines. Moreover, decreased expression of miR-4262 was closely related to increased expression of Kaiso (ZBTB33). PMID: 28800784
    2. extracellular microenvironment signals regulate RhoH and Kaiso to modulate actin-cytoskeleton structure and transcriptional activity during T cell migration PMID: 27574848
    3. Kaiso silencing had similar effects as miR-181a overexpression in glioma cells, whereas overexpression of Kaiso in glioma cells partially reversed the inhibitory effects of the miR-181a mimic. PMID: 30036882
    4. data show that Kaiso favors an mCpG over a CpG site by 2 orders of magnitude in affinity and that an important component of this effect is the presence of hydrophobic and CH...O contacts involving E535. PMID: 29546986
    5. High levels of nuclear Kaiso expression were detected in patients with a higher degree of African heritage compared to their Caucasian counterparts, suggesting a role for Kaiso in triple-negative breast cancer racial disparity. PMID: 28887687
    6. Kaiso promotes Jagged-1 expression, which may have implications in Notch-mediated colon cancer progression PMID: 28637464
    7. A role for Kaiso in the proliferation. PMID: 28333150
    8. the pathological features of the metastatic activity of parental (Kaisopositive) versus Kaisodepleted MDA-231 cells, are reported. PMID: 28880889
    9. Suggest a role for Kaiso in the progression of pancreatic ductal adenocarcinomas, involving the epithelial mesenchymal transformation markers, E-cadherin and Zeb1. PMID: 27424525
    10. a novel mechanism by which ZBTB33 mediates the cyclin D1/cyclin E1/RB1/E2F pathway, controlling passage through the G1 restriction point and accelerating cancer cell proliferation. PMID: 27694442
    11. GR is a putative target gene of Kaiso. PMID: 26424557
    12. Kaiso high expression correlated with invasion of prostate cancer. PMID: 26734997
    13. Results show that Kaiso binding to unmethylated Kaiso binding site in the human ICR1 is necessary for ICR1 methylation maintenance and affects transcription rates of the lncRNA H19. PMID: 27152123
    14. Kaiso modulates HIF1A gene expression by binding to the methylated HIF1A promoter in a region proximal to the autoregulatory HIF-1 binding site, primarily during hypoxia. PMID: 26514431
    15. NF-kappaB response element, located close to the p53RE#1, mediates APAF1 transcriptional repression by affecting interaction between KAISO and p53 PMID: 26183023
    16. KAISO is a regulator of p53-mediated transcription of CDKN1A and apoptotic genes PMID: 25288747
    17. Data indicate that Kaiso protein participates in the regulation of beta-catenin mRNA expression by interacting with p120-catenin in the lung cancer cell lines. PMID: 24498333
    18. Nuclear localization of Kaiso promotes the poorly differentiated phenotype and EMT in infiltrating ductal carcinomas. PMID: 24570268
    19. The optimized knockdown with p120 and Kaiso siRNAs further expands the size of HCEC monolayers without endothelial mesenchymal transition (EMT) via selective activation of p120/Kaiso signaling that requires the RhoA-ROCK-noncanonical BMP-NFkB signaling. PMID: 24474278
    20. Transcription factor Kaiso does not interact with hydroxymethylated DNA within CTGCNA sequence context PMID: 23888785
    21. presence of MTG16 in this complex, and its contributions to transcriptional repression both required Kaiso binding to its binding site on DNA, establishing MTG16-Kaiso binding as functionally relevant in Kaiso-dependent transcriptional repression PMID: 23251453
    22. Kaiso represses the cell cycle gene cyclin D1 via sequence-specific and methyl-CpG-dependent mechanism. PMID: 23226276
    23. These findings establish a defined oncogenic role of Kaiso in promoting the progression of prostate cancer. PMID: 22974583
    24. High cytoplasmic kaiso expression is associated with thymoma. PMID: 22833212
    25. nuclear Kaiso is common in clinically aggressive ductal breast cancer, while cytoplasmic Kaiso and a p120-mediated relief of Kaiso-dependent transcriptional repression characterize invasive lobular carcinoma (ILC). PMID: 22662240
    26. Molecular basis for recognition of methylated and specific DNA sequences by the zinc finger protein Kaiso PMID: 22949637
    27. Selective activation of p120ctn-Kaiso signaling to unlock contact inhibition of ARPE-19 cells without epithelial-mesenchymal transition. PMID: 22590627
    28. Data show that Kaiso requires all three zinc fingers plus adjacent protein regions for DNA recognition. PMID: 22300642
    29. Results suggest that p120ctn isoforms 1 and 3 up-regulate cyclin D1, and thereby cyclin E, resulting in the promotion of cell proliferation and cell cycle progression in lung cancer cells. PMID: 22276175
    30. when released from E-cadherin by Wnt3a-stimulated phosphorylation, p120-catenin controls the activity of Kaiso, enhancing its binding to repressed promoters and relieving its inhibition of the beta-catenin-Tcf-4 transcriptional complex. PMID: 21670201
    31. Increased delta-catenin expression is critical for maintenance of the malignant phenotype of lung cancer, making delta-catenin a candidate target protein for future cancer therapeutics. PMID: 21070476
    32. In endothelial cells, p120ctn has a transcription repression function through regulation of Kaiso, possibly as a cofactor with the transcription factor. PMID: 20382170
    33. Data strongly imply that Kaiso's function as a transcriptional regulator might be linked to the control of the cell cycle and to cell proliferation in cancer. PMID: 20169156
    34. Bifunctional role of domain zinc fingers of methyl-DNA-binding protein Kaiso PMID: 20586187
    35. differential expression of p120(ctn) and Kaiso mRNA was observed in human coronary artery endothelial cells depending on how laminar shear stress was applied in relation to the wounding process PMID: 14699141
    36. Kaiso translocates to the nucleus to regulate transcription of genes with diverse roles in cell growth and development PMID: 15564377
    37. Data imply an unexpected influence of the microenvironment on Kaiso expression and localizationin colorectal adenocarcinoma. PMID: 15781635
    38. Kaiso associates with the matrilysin promoter in vivo. PMID: 15817151
    39. KAISO binds specifically to the methylated, but not the unmethylated, sequence in the first exon of the tyrosine hydroxylase [TH]gene and thus may play an important role in modulating human TH gene expresison. PMID: 15953356
    40. the Kaiso-CTCF interaction negatively regulates CTCF insulator activity PMID: 16230345
    41. Our study suggests that this gene is not implicated in the RTT molecular pathogenesis PMID: 16530985
    42. p120 and Kaiso regulate Helicobacter pylori-induced expression of matrix metalloproteinase-7 PMID: 18653469
    43. Kaiso represses methylated tumor suppressor genes and can bind in a methylation-dependent manner to the CDKN2A in human colon cancer cell lines. Kaiso depletion induced tumor suppressor gene expression without affecting DNA methylation levels. PMID: 18794111
    44. Kaiso plays some specific role in centrosome assembly and function, which is not restricted to mitosis PMID: 19502788
    45. Data suggest cytoplasmic Kaiso expression is associated with poor prognosis of NSCLC and various subcellular localizations of Kaiso may play differential biological roles in NSCLC. PMID: 19508730

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Note=Also cytoplasmic in cells grown at high densities.
  • 组织特异性：
    Expressed in vascular endothelium.
  • 数据库链接：

    HGNC: 16682

    OMIM: 300329

    KEGG: hsa:10009

    STRING: 9606.ENSP00000314153

    UniGene: Hs.143604