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  4. Recombinant Human Transcriptional activator protein Pur-alpha (PURA)

Recombinant Human Transcriptional activator protein Pur-alpha (PURA)

  • 货号：
    CSB-YP019083HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP019083HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP019083HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP019083HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP019083HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PURA
  • Uniprot No.：
  • 别名：
    PUR ALPHA; PUR1; purA; PURA_HUMAN; PURALPHA; Purine rich element binding protein A; Purine rich single stranded DNA binding protein alpha; Purine-rich single-stranded DNA-binding protein alpha; Transcriptional activator protein PUR alpha; Transcriptional activator protein Pur-alpha
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-322
  • 氨基酸序列
    MADRDSGSEQ GGAALGSGGS LGHPGSGSGS GGGGGGGGGG GGSGGGGGGA PGGLQHETQE LASKRVDIQN KRFYLDVKQN AKGRFLKIAE VGAGGNKSRL TLSMSVAVEF RDYLGDFIEH YAQLGPSQPP DLAQAQDEPR RALKSEFLVR ENRKYYMDLK ENQRGRFLRI RQTVNRGPGL GSTQGQTIAL PAQGLIEFRD ALAKLIDDYG VEEEPAELPE GTSLTVDNKR FFFDVGSNKY GVFMRVSEVK PTYRNSITVP YKVWAKFGHT FCKYSEEMKK IQEKQREKRA ACEQLHQQQQ QQQEETAAAT LLLQGEEEGE ED
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene. May play a role in the initiation of DNA replication and in recombination.
  • 基因功能参考文献：
    1. Three genes in our epilepsy cohort (COQ4, DNM1, and PURA), accounting for 14% (3/21) of all novel genetic etiologies identified in patients with epilepsy, were subsequently confirmed in independent publications. PMID: 26795593
    2. PURA may be a potential target of miR-144 and observed downregulation of PURA may be caused by increased expression of miR-144. The other predicted target of miR-144 SPRED1, was found to be downregulated in 69 per cent EC tissues as compared to matched distant non-malignant tissues. PMID: 27748283
    3. The results of this study emphasizes the importance of stress granules in ALS pathogenesis and identifies Pur-alpha as a novel regulator of cytoplasmic stress granules dynamics. PMID: 26728149
    4. These findings provide definitive evidence for the role of PURA in causing a variable syndrome of neurodevelopmental delay, learning disability, neonatal hypotonia, feeding difficulties, abnormal movements and epilepsy in humans PMID: 25342064
    5. Mutations in PURA cause profound neonatal hypotonia, seizures, and encephalopathy in 5q31.3 microdeletion syndrome. PMID: 25439098
    6. Data show that protein PURalpha is specifically involved in the transcriptional activation of the secondary promoter and may exert its function by forming a complex with E2F-1 and RNA polymerase II. PMID: 24819879
    7. Suggest dynamic interplay between transcriptional activators Pur-alpha/Pur-beta and repressors in regulating SMalphaA gene output during myofibroblast differentiation. PMID: 24446247
    8. This study showed that Pur-alpha showed inadequate expression in monocytes, and the translation of Pur-alpha mRNA was repressed by cell-expressed microRNA. PMID: 22835829
    9. The study identifies three functionally distinct PURA promoters and show that these are utilized differentially in human cell types and that they respond differently to cytomegalovirus infection. PMID: 21062477
    10. The ability of Puralpha to activate the CD11c gene promoter increases in differentiating U937 monocytic cells. PMID: 11937543
    11. acts together with hnRNP-K to repress the transcriptional activity of the CD43 gene promoter during lymphocyte activation PMID: 12411317
    12. results show that PUR proteins are capable of binding to alpha-MHC mRNA and attenuate its translational efficiency; also show robust expression of PUR proteins in failing hearts where alpha-MHC mRNA levels are suppressed PMID: 12933792
    13. The N-terminal 72 amino acids of Pura were involved in E2F-1 binding, inhibition of promoter activation by E2F-1 and reversal of E2F-mediated growth inhibition. PMID: 15517862
    14. Puralpha has been shown to colocalize with cyclin A/Cdk2 and to coimmunoprecipitate with cyclin A during S-phase and we show that this interaction is mediated by a specific affinity of Puralpha for Cdk2. PMID: 15707957
    15. Hypoxia induces a coordinated up-regulation in beta2 integrin expression that is dependent upon transcriptional mechanisms mediated by HIF-1 and Pur alpha. PMID: 17641060
    16. the partnership of Puralpha with Rev is beneficial for Rev-mediated expression of the HIV-1 genome. PMID: 17722108
    17. that the recruitment of YB1, PURalpha, and H1.2 to the p53 target gene Bax is required for repression of p53-induced transcription. PMID: 18258596
    18. Stably overexpressing PURalpha in PC3 and DU145 cells negatively regulates cell proliferation, resulting in decreases in PCNA expression PMID: 18386260
    19. A transcriptional repressor complex that binds a specific sequence (repressor element) in the androgen recptor gene 5'-untranslated region in prostate cancer contains Pur alpha. PMID: 18413735
    20. Pur-alpha protein is associated with Alzheimer's disease. PMID: 18780968
    21. Effects of the DNA damage-inducing cancer chemotherapeutic agent cisplatin on mouse embryo fibroblasts (MEFs) from PURA negative knockout mice. PMID: 18927497
    22. Regulation of PURalpha expression in prostate cancer cells may serve as a therapeutic target for hormone refractory prostate cancer. PMID: 19267365

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  • 相关疾病：
    Mental retardation, autosomal dominant 31 (MRD31)
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    PUR DNA-binding protein family
  • 数据库链接：

    HGNC: 9701

    OMIM: 600473

    KEGG: hsa:5813

    STRING: 9606.ENSP00000332706

    UniGene: Hs.443121