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  4. Recombinant Human Transcription factor E2F3 (E2F3)

Recombinant Human Transcription factor E2F3 (E2F3)

  • 货号：
    CSB-YP007342HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP007342HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP007342HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP007342HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP007342HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    DKFZp686C18211; E2F 3; E2F transcription factor 3; E2F-3; E2F3; E2F3_HUMAN; KIAA0075; MGC104598; Transcription factor E2F 3; Transcription factor E2F3
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-465
  • 氨基酸序列
    MRKGIQPALE QYLVTAGGGE GAAVVAAAAA ASMDKRALLA SPGFAAAAAA AAAPGAYIQI LTTNTSTTSC SSSLQSGAVA AGPLLPSAPG AEQTAGSLLY TTPHGPSSRA GLLQQPPALG RGGSGGGGGP PAKRRLELGE SGHQYLSDGL KTPKGKGRAA LRSPDSPKTP KSPSEKTRYD TSLGLLTKKF IQLLSQSPDG VLDLNKAAEV LKVQKRRIYD ITNVLEGIHL IKKKSKNNVQ WMGCSLSEDG GMLAQCQGLS KEVTELSQEE KKLDELIQSC TLDLKLLTED SENQRLAYVT YQDIRKISGL KDQTVIVVKA PPETRLEVPD SIESLQIHLA STQGPIEVYL CPEETETHSP MKTNNQDHNG NIPKPASKDL ASTNSGHSDC SVSMGNLSPL ASPANLLQQT EDQIPSNLEG PFVNLLPPLL QEDYLLSLGE EEGISDLFDA YDLEKLPLVE DFMCS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters.
  • 基因功能参考文献：
    1. Results suggest that E2F3b isoform functions as an important promoter for cell proliferation and plays important roles in transcriptional regulation in HepG2 liver cancer cells. PMID: 29135049
    2. Mechanistic investigations suggested that hsa_circ_0008039 served as a competing endogenous RNA (ceRNA) of miR-432-5p. Subsequently, E2F3 was identified as the functional target of miR-432-5p and overexpression of hsa_circ_0008039 elevated E2F3 expression in breast cancer. PMID: 29807010
    3. miR433 may inhibit the progression of nonsmallcell lung cancer, at least in part, by targeting E2F3. The present study indicated that miR433 may be investigated as an innovative candidate target for the therapy of patients with this fatal disease. PMID: 29767254
    4. our findings indicated that miR-152 reduced osteosarcoma growth and invasion by targeting E2F3 PMID: 28810933
    5. these results revealed that miR-363 is involved in hepatocellular carcinoma (HCC)growth and invasion and functions as a tumor suppressor by negatively regulating E2F3. PMID: 29039555
    6. E2F3 and ephrin A3 are putative targets of miR-210, and their protein expression was up-regulated in the angiosarcoma cells. PMID: 28739548
    7. The increased expression of miR-503-5p significantly reduced the expressions of E2F transcription factor 3 (E2F3) mRNA and retinoblastoma protein (Rb)/E2F signaling pathway mRNA in bladder cancer cells. PMID: 29169421
    8. Results of real-time PCR and Western blot showed that the depletion of E2F3a upregulated the expression levels of cell apoptosis-related proteins and downregulated migration-related proteins. Conversely, E2F3a overexpression downregulated the expression levels of cell apoptosis-related proteins and upregulated migration-related proteins. PMID: 28337965
    9. lncRNA H19 may function as the sponge of miR-106a-5p to up-regulate E2F3 expression, and consequently promote the glucose metabolism and growth of melanoma. PMID: 29350287
    10. Blood E2F3 mRNA is a sensitive diagnostic marker in lung cancer; moreover, it is a promising prognostic marker capable of efficiently discriminating early from late stages of the disease PMID: 28269748
    11. miR128-1 could function as a tumor suppressor in glioblastoma multiforme by negatively regulating tumor cell proliferation, invasion and self-renewal through direct targeting BMI1 and E2F3. PMID: 27705931
    12. NEAT1 modulated the expression of E2F3 and MiR377 and promoted non small cell lung cancer progression. PMID: 27351135
    13. High expression level of E2F3 is associated with lung adenocarcinoma. PMID: 26942465
    14. the current study demonstrated that miR34a suppressed the proliferation and induced apoptosis of SRA01/04 cells by downregulating E2F3. PMID: 27840975
    15. we investigated how Rb is integrated to control bladder cancer progression through E2F3 and p53 regulation. The results exhibit that Rb expression is lower in patients with bladder tumor, while E2F3 level is high. Rb knockdown enhanced bladder tumor cell proliferation and migration, aggravated with p53 silence. PMID: 27922689
    16. By downregulating E2F3 (an miR-497 target gene) mRNA and protein. PMID: 27430325
    17. miR-874 expression was significantly decreased in OS cell lines and tissues, and its expression was correlated with TNM stage, tumor size, and lymph node metastasis; miR-874 inhibited cell proliferation, migration, and invasion and induced cell apoptosis of OS cells partially by targeting E2F3. PMID: 26631042
    18. miR-503 inhibits cell proliferation and induces apoptosis by directly targeting E2F3 in colorectal cancer cells. PMID: 26722476
    19. E2F3 silencing decreases Her2-positive mammary tumor growth by reducing percentage of cells undergoing mitosis. PMID: 26512919
    20. mirn424 has a role in inhibiting Akt3/E2F3 axis and tumor growth in hepatocellular carcinoma PMID: 26315541
    21. miR-145 expression was lower in tumors compared with matched normal samples and correlated with increased the E2F3 transcription factor protein staining. PMID: 25762621
    22. miR-200c appears to control the epithelial-to-mesenchymal transition process through BMI-1 in bladder cancer cells, and it inhibits their proliferation through down-regulating E2F3. PMID: 25367080
    23. In gastric cancer miR-141 could be interacting with MEG3 and targeting E2F3 to inhibit cell proliferation. PMID: 26233544
    24. our study demonstrated that miR-34c plays a role of tumor suppressor in HEC-1-B cells, and E2F3 protein may be a target of miR-34c. PMID: 25846116
    25. MiR-377 is an important negative regulator of E2F and MAP3K7/NF-kB signaling pathway in melanoma cells. PMID: 25889255
    26. MiR-203 sensitizes glioma cells to temozolomide and inhibits glioma cell invasion by targeting E2F3. PMID: 25515700
    27. Data suggest that aberrant cell cycle activation in Ewing sarcoma is due to the de-repression of transcription factor E2F targets of transcriptional induction and physical recruitment of E2F3 by fusion protein EWS-FLI1 replacing E2F4 on their promoters. PMID: 25712098
    28. E2F3 may be a potential target gene of miR-125a-5p in gastric cancer. PMID: 25231560
    29. High E2F3 expression is associated with hepatocellular carcinoma. PMID: 25073510
    30. miR-141 suppresses the growth and metastasis of HCC cells by targeting E2F3. PMID: 25142234
    31. findings suggest miR-217 function as a potential tumor suppressor in HCC progression and miR-217-E2F3 axis may be a novel candidate for developing rational therapeutic strategies PMID: 24671492
    32. HLM006474 synergizes with paclitaxel in lung cancer cell lines to inhibit E2F PMID: 24831239
    33. novel HDAC1/4/miR-200b/E2F3 signaling contributes to chemoresistance of human lung adenocarcinoma cells PMID: 24830600
    34. autoregulatory feedback loop of EZH2/miR-200c/E2F3 served an important function in prostate cancer development PMID: 25017995
    35. Overexpression of E2F3 may be associated with unfavorable prognosis in hepatocellular carcinoma. PMID: 24402082
    36. Wilms' tumors with aggressive features, such as higher stage, unfavorable histology and higher risk level, expressed higher levels of E2F3 protein and mRNA. PMID: 24210184
    37. Studies show that a large fraction of E2F3 target genes are synergistically coregulated by aberrant ETS proteins. PMID: 23940108
    38. two cell cycle-related molecules, cyclin D3 and E2F3, were identified as the direct miR-503 targets. PMID: 23967867
    39. Studies revealed that E2F3 was a direct target of miR-449a in lung cancer cells. miR-449a also suppressed tumor formation in vivo in nude mice. These results suggest that miR-449a plays an important role in lung cancer tumorigenesis. PMID: 24211326
    40. Overexpression of E2F3 promotes proliferation of functional human beta cells without induction of apoptosis. PMID: 23907129
    41. The correlation between CCND1, E2F3 and miR-449b showed that miR-449b could downregulate CCND1 and E2F3 expression. This, in turn, reduced the proliferative ability of colon cancer stem cells. PMID: 23674142
    42. down-regulation of E2f3 with age helps drive the dramatic decline in Igf2 expression in postnatal organs, and E2F3 overexpression in cancer induces IGF2 overexpression PMID: 23530192
    43. Studied E2F3 and its two isoforms E2F3a and E2F3b in wild-type ovarian carcinomas and ovarian carcinomas associated with germline BRCA1 and BRCA2 mutations. PMID: 22887716
    44. findings authenticate the efficiency of siRNA (E2F3) to fight against breast cancer; hence, the siRNA mediated E2F3 gene silencing knockdown the E2F3 PMID: 22960857
    45. Data suggest that microRNA/mRNA pairs in hsa-miR-140-3p/RAD51AP1/, hsa-miR-145/E2F3, hsa-miR-139-5p/TOP2A, and hsa-miR-133a/GCLC were correlated with ovarian tumorigenesis. PMID: 22452920
    46. Trim28 regulates cell proliferation by bridging HDAC1 and E2F3 and E2F4 interactions. PMID: 23060449
    47. A homology model of DNA-binding domain of transcription factor E2F3 was generated for the design of selective ligands. PMID: 23009996
    48. E2F3 controls the ubiquitination of PCNA through the transcriptional regulation of Rad18. PMID: 22391204
    49. Our results suggest that down-regulation of miR-200b could lead to E2F3 overexpression and in turn contribute to chemoresistance of lung adenocarcinoma cells to docetaxel. PMID: 22139708
    50. miR-203 induced cellular senescence by targeting E2F3 in human melanoma Mewo and A2058 cells PMID: 22354972

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    E2F/DP family
  • 数据库链接：

    HGNC: 3115

    OMIM: 600427

    KEGG: hsa:1871

    STRING: 9606.ENSP00000262904

    UniGene: Hs.269408