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  4. Recombinant Human Toll-like receptor 8 (TLR8), partial

Recombinant Human Toll-like receptor 8 (TLR8), partial

  • 货号：
    CSB-EP023607HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP023607HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP023607HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP023607HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    CD 288; CD288; CD288 antigen; MGC119599; MGC119600; TLR 8; Tlr8; TLR8_HUMAN; Toll like receptor 8; Toll-like receptor 8
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 表达区域：
    27-827
  • 氨基酸序列
    EENFSRSYPCDEKKQNDSVIAECSNRRLQEVPQTVGKYVTELDLSDNFITHITNESFQGLQNLTKINLNHNPNVQHQNGNPGIQSNGLNITDGAFLNLKNLRELLLEDNQLPQIPSGLPESLTELSLIQNNIYNITKEGISRLINLKNLYLAWNCYFNKVCEKTNIEDGVFETLTNLELLSLSFNSLSHVPPKLPSSLRKLFLSNTQIKYISEEDFKGLINLTLLDLSGNCPRCFNAPFPCVPCDGGASINIDRFAFQNLTQLRYLNLSSTSLRKINAAWFKNMPHLKVLDLEFNYLVGEIASGAFLTMLPRLEILDLSFNYIKGSYPQHINISRNFSKLLSLRALHLRGYVFQELREDDFQPLMQLPNLSTINLGINFIKQIDFKLFQNFSNLEIIYLSENRISPLVKDTRQSYANSSSFQRHIRKRRSTDFEFDPHSNFYHFTRPLIKPQCAAYGKALDLSLNSIFFIGPNQFENLPDIACLNLSANSNAQVLSGTEFSAIPHVKYLDLTNNRLDFDNASALTELSDLEVLDLSYNSHYFRIAGVTHHLEFIQNFTNLKVLNLSHNNIYTLTDKYNLESKSLVELVFSGNRLDILWNDDDNRYISIFKGLKNLTRLDLSLNRLKHIPNEAFLNLPASLTELHINDNMLKFFNWTLLQQFPRLELLDLRGNKLLFLTDSLSDFTSSLRTLLLSHNRISHLPSGFLSEVSSLKHLDLSSNLLKTINKSALETKTTTKLSMLELHGNPFECTCDIGDFRRWMDEHLNVKIPRLVDVICASPGDQRGKSIVSLELTTCVSDVT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Endosomal receptor that plays a key role in innate and adaptive immunity. Controls host immune response against pathogens through recognition of RNA degradation products specific to microorganisms that are initially processed by RNASET2. Recognizes GU-rich single-stranded RNA (GU-rich RNA) derived from SARS-CoV-2, SARS-CoV-1 and HIV-1 viruses. Upon binding to agonists, undergoes dimerization that brings TIR domains from the two molecules into direct contact, leading to the recruitment of TIR-containing downstream adapter MYD88 through homotypic interaction. In turn, the Myddosome signaling complex is formed involving IRAK4, IRAK1, TRAF6, TRAF3 leading to activation of downstream transcription factors NF-kappa-B and IRF7 to induce proinflammatory cytokines and interferons, respectively.
  • 基因功能参考文献：
    1. Conditioned media or microparticles released from obese omental adipose tissue increased CD16 and CCR5 expression on CD14(+)CD16(-) monocytes and augmented their migratory capacity towards the conditioned media from obese omental adipose tissue, itself. PMID: 27677832
    2. MBZ-induced IL-1beta release was found to be dependent on NLRP3 inflammasome activation and to involve TLR8 stimulation. PMID: 28472897
    3. the current study shows that motolimod ( selective small-molecule agonist of TLR8, stimulates natural killer (NK) cells, dendritic cells, and monocytes))can be safely administered in combination with cetuximab with an acceptable toxicity profile PMID: 27810904
    4. Epstein-Barr virus (EBV) replication activating the toll like receptor 8 (TLR8) molecular pathway in primary monocytes. PMID: 28245863
    5. this study identified TLR8 as mediator of monocyte differentiation and M2 macrophage polarization during hepatitis C virus infection PMID: 28122964
    6. Demonstrate STAT1-dependent transcriptional activation of TLR8 with estrogen stimulation. PMID: 28039018
    7. TLR8 gene rs3764880 polymorphisms might be associated with susceptibility to ischemic stroke and involved in the inflammatory reaction and lipid metabolism of ischemic stroke in southern Chinese Han. PMID: 27772795
    8. this study shows that TLR8 gene polymorphism is associated with susceptibility to HIV and HCV co-infection PMID: 28062211
    9. Comparison of present and previous association studies reveals contradictory results for common variants. Thus, no associations exist between genetic variation in TLR8 and AR. PMID: 26556310
    10. Identified human TLR8 as functional TLR13 equivalent that promiscuously senses ssRNA. PMID: 26545385
    11. TLR8 polymorphisms seem to be related to non-progression of liver fibrosis in HIV/HCV coinfected patients, particularly in males and those patients infected with GT1. PMID: 26455634
    12. TLR8 with the uncleaved Z-loop is unable to form a dimer. The uncleaved Z-loop located on the ascending lateral face prevents the approach of the dimerization partner by steric hindrance. PMID: 26929371
    13. Sex-specific associations for TLR8 polymorphisms in tuberculosis. PMID: 26160538
    14. Knockdown of LRRC59 reduced TLR3-, 8-, and 9-mediated, but not TLR4-mediated, signaling. PMID: 26466955
    15. the activation of TLR8 by bi-specific si-RNA for the production of INFbeta, was investigated. PMID: 26202481
    16. Results suggest a relationship between the TLR8 and the susceptibility to Crohn's disease and Behcet's disease. PMID: 26486764
    17. Carriage of the G-allele of TLR8-rs3764879 was associated with the occurrence of SLE in Danish women, but not men. PMID: 24919757
    18. TLR8 is identified as receptor for bacterial RNA in primary human monocyte-derived macrophages. Moreover, TLR8-dependent detection of bacterial RNA was critical for triggering monocyte activation in response to infection with Streptococcus pyogenes. PMID: 26101323
    19. This study demonstrates a physiological role of TLR8 in the sensing of entire S. aureus in human primary phagocytes, including the induction of IFN-beta and IL-12 production via a TAK1-IKKbeta-IRF5 pathway that can be inhibited by TLR2 signaling. PMID: 26085680
    20. No significant associations have been found between TLR8 polymorphisms and susceptibility to Graves' disease in Chinese Cantonese. PMID: 25430040
    21. Met1Val of TLR8 is significantly associated with risk of pulmonary tuberculosis susceptibility in southern Punjab population. This effect was highly pronounced among males. PMID: 25572425
    22. The minor allele of TLR8 rs3764879 of the donor is associated with outcome after myeloablative conditioned allogeneic hematopoietic cell transplantation. PMID: 25464115
    23. TLR8, but not TLR7, is involved in priming of human neutrophil reactive oxygen species production by inducing the phosphorylation of p47phox and p38 MAPK. PMID: 25877926
    24. Increased miR-K-10b and miR-K12-12 are functionally involved in sepsis as agonists of TLR8, forming a positive feedback that may lead to cytokine dysregulation. PMID: 25476907
    25. Data show that activation of toll-like receptor 8 (TLR8) signaling in tumor cells can block the induction and reverse the suppression of senescent naive and tumor-specific T cells in vitro and in vivo, resulting in enhanced anti-tumor immunity. PMID: 25231413
    26. Data indicate that treatment with toll-like receptor 8 (TLR8) agonists elicited granzyme B production. PMID: 25667415
    27. TLR8 activation has direct anti-leukemic effects independent of its immunomodulating properties. PMID: 25283842
    28. The insertion loop between leucine-rich repeats 14 and 15 in TLR8 is indispensable for the receptor cleavage in endosomes. PMID: 25297876
    29. HIV-1 infection induced the expression of pro-IL-1beta via TLR8-mediated mechanisms and activated caspase-1 through the NLRP3 inflammasome to cleave pro-IL-1beta into bioactive IL-1beta PMID: 24939850
    30. The mRNA expression level of TLR8 in the patients with cervical cancer and Hela cells were up-regulated, it consistent with the increased expression of VEGF and Bcl-2. PMID: 24782650
    31. Data indicate 4-methyl-1-pentyl-1H-benzo[d]imidazol-2-amine a pure toll-like receptor 8 (TLR8) agonist, evoking proinflammatory cytokine and Type II interferon responses in mononuclear leukocytes. PMID: 25102141
    32. non-synonymous TLR8 SNP rs3764880-G allele was a risk factor for oral ulcer and pericardial effusion, with significant additive effects PMID: 24445780
    33. Variations in TLR8 genes may modulate immune responses during HCV infection. PMID: 24205871
    34. TLR8 is a novel estrogen target gene that can lower the inflammatory threshold and implicate an IFNalpha-independent inflammatory mechanism that could contribute to higher systemic lupus erythematosus incidence in women. PMID: 24525049
    35. The mechanisms of action of Aldaratrade mark are complex. Imiquimod is an effective ligand for TLR7 (and TLR8 in humans) and also interferes with adenosine receptor signaling. PMID: 24254490
    36. Bb RNA is a TLR8 ligand in human monocytes and that transcription of IFN-beta in response to the spirochete is induced from within the phagosomal vacuole through the TLR8-MyD88 pathway. PMID: 23906644
    37. Data indicate that TLR8 stimulation of NK-DC co-cultures significantly increased DC priming of EGFR-specific CD8(+) T cells in the presence of cetuximab. PMID: 23685782
    38. TLR8 expression and signaling is impaired in peripheral blood mononuclear cells from patients with multiple sclerosis PMID: 23787171
    39. This study demonstrates profibrotic properties of circulating monocytes from patients with systemic sclerosis and a key role for TLR signalling, particularly TLR8, in TIMP-1 secretion and matrix remodelling. PMID: 23223421
    40. crystal structures of unliganded and ligand-induced activated TLR8 dimers were elucidated; ligand binding induces reorganization of the TLR8 dimer, which enables downstream signaling processes PMID: 23520111
    41. Single nucleotide polymorphism in the TLR8 gene is associated with allergic rhinitis. PMID: 22857391
    42. IL10 production after TLR9 ligands or HSV-1 stimulation was significantly related with plasma levels of sex hormones, whereas no correlation was found in cytokines produced following TLR7 and TLR8 stimulation. PMID: 22768144
    43. Expression of TLR8 is significantly increased in HeLa cells. TLR8 agonist may influence tumor development. PMID: 22340042
    44. UNC93B1 physically associates with human TLR8 and regulates TLR8-mediated signaling PMID: 22164301
    45. Epigenetic regulation of tumor necrosis factor alpha (TNFalpha) release in human macrophages by HIV-1 single-stranded RNA (ssRNA) is dependent on TLR8 signaling PMID: 22393042
    46. analyzed the frequency of TLR7 rs179008, TLR8 rs3764880, TLR9 rs5743836 and rs352140 in 370 patients with SLE and 415 healthy controls from southern Brazil PMID: 22065095
    47. Human TLR8 detectS viral single-stranded RNA and imidazoquinoline compounds. Review. PMID: 22289837
    48. Quantitative polymerase chain reaction showed significantly higher expression of LEAP-1 (P = 0.002) and TLR-8 (P = 0.023) and TLR-10 (P = 0.014) in viral keratitis and LEAP-2 (P = 0.034) in dry eye, versus controls. PMID: 21499082
    49. Lower toll-like receptor 8 expression was observed in monocytes from infants with respiratory syncytial virus infection. PMID: 20946625
    50. Phagosomal signaling involves a cooperative interaction between TLR2 and TLR8 in pro- and antiinflammatory cytokine responses, whereas TLR8 is solely responsible for IRF7-mediated induction of IFN-beta. PMID: 21321205

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  • 亚细胞定位：
    Endosome membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Toll-like receptor family
  • 组织特异性：
    Expressed in myeloid dendritic cells, monocytes, and monocyte-derived dendritic cells.
  • 数据库链接：

    HGNC: 15632

    OMIM: 300366

    KEGG: hsa:51311

    STRING: 9606.ENSP00000218032

    UniGene: Hs.660543