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  4. Recombinant Human Toll-interacting protein (TOLLIP)

Recombinant Human Toll-interacting protein (TOLLIP)

  • 货号：
    CSB-YP875640HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP875640HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP875640HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP875640HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP875640HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Adapter protein; FLJ33531; IL 1RAcPIP; IL1RAcPIP; TOLIP; TOLIP_HUMAN; Toll interacting protein; Toll-interacting protein; tollip
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-274
  • 氨基酸序列
    ATTVSTQRG PVYIGELPQD FLRITPTQQQ RQVQLDAQAA QQLQYGGAVG TVGRLNITVV QAKLAKNYGM TRMDPYCRLR LGYAVYETPT AHNGAKNPRW NKVIHCTVPP GVDSFYLEIF DERAFSMDDR IAWTHITIPE SLRQGKVEDK WYSLSGRQGD DKEGMINLVM SYALLPAAMV MPPQPVVLMP TVYQQGVGYV PITGMPAVCS PGMVPVALPP AAVNAQPRCS EEDLKAIQDM FPNMDQEVIR SVLEAQRGNK DAAINSLLQM GEEP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. Connects the ubiquitin pathway to autophagy by functioning as a ubiquitin-ATG8 family adapter and thus mediating autophagic clearance of ubiquitin conjugates. The TOLLIP-dependent selective autophagy pathway plays an important role in clearance of cytotoxic polyQ proteins aggregates.
  • 基因功能参考文献：
    1. Our results indicate that polymorphisms in TOLLIP influence the risk of pulmonary tuberculosis disease. PMID: 29924447
    2. Data reveal a novel mechanism in Tollip alteration that underlies the inflamed and incompetent polarization of neutrophils leading to severe outcomes of septic colitis. PMID: 27703259
    3. Our data suggest that Tollip SNP rs5743899 may predict varying airway response to RV infection in asthma. PMID: 27513438
    4. pharmacogenetic analysis of patients enrolled in an idiopathic pulmonary fibrosis (IPF) clinical trial identified a variant within TOLLIP to be associated with differential response to N-acetylcysteine therapy. PMID: 27253772
    5. study to examine potential associations between a selection of SNPs in the genes encoding TLR2 and TOLLIP, and predisposition, severity and outcome of Staphylococcus aureus bloodstream infections (SABSI); the TLR2 and TOLLIP polymorphisms were not associated with susceptibility to SABSI, severity, 30-day all-cause mortality, or SABSI caused by the clonal complex 30 genotype PMID: 28736863
    6. Toll-interacting protein (TOLLIP) is a ubiquitin-binding protein that regulates innate immune responses. PMID: 28463648
    7. Knock-down of Tollip promotes HIV-1 reactivation from latency. PMID: 27181351
    8. Toll-interacting protein rs5743867 polymorphism tends to decrease the risk of sepsis in infants undergoing complex open heart surgery. PMID: 27002100
    9. Variants in the TOLLIP gene are associated with higher circulating PAI-1 plasma levels and association with clinical Primary Graft Dysfunction Risk. PMID: 26663441
    10. Tom1 modulates binding of Tollip to phosphatidylinositol 3-phosphate via a coupled folding and binding mechanism. PMID: 26320582
    11. These data suggest that M. leprae upregulates IL-1Ra by a TOLLIP-dependent mechanism; inhibition of TOLLIP may decrease an individual's susceptibility to leprosy and offer a novel therapeutic target for IL-1-dependent diseases. PMID: 26610735
    12. Observed expression patterns of several TLR inhibitory proteins with a noticeable suppression in expression of two of these; PPARgamma and TOLLIP in both ulcerative colitis and Crohn's disease and in both active and inactive disease states. PMID: 26462859
    13. Tollip acts as a novel modulator of I/R injury by promoting neuronal apoptosis and ischaemic inflammation, which are largely mediated by suppression of Akt signalling. PMID: 26011492
    14. The two polymorphisms, rs5743899 and rs3750920, in the TOLLIP gene are independently associated with an increased risk of developing cutaneous leishmaniasis (CL). PMID: 26107286
    15. TOLLIP encodes toll-interacting protein (TOLLIP), which is an inhibitory adaptor protein acting downstream from the toll-like receptors (TLRs). Read More: http://www.atsjournals.org/doi/full/10.1164/rccm.201505-1010OC#.VwqiYdLrvyA PMID: 26331942
    16. we identify a novel function of Tollip in regulating the canonical Wnt pathway which is evolutionarily conserved between fish and humans. Tollip-mediated inhibition of Wnt signaling may contribute to embryonic development and to carcinogenesis. PMID: 26110841
    17. TOLLIP contributes to mortality following myocardial infarction through promoting inflammation and apoptosis PMID: 25765712
    18. Study shows that host factor Tollip inhibits HIV LTR-driven gene expression by suppressing NF-kappaB activation revealing its novel role in modulating HIV-1 infection. PMID: 25915421
    19. Which is achieved by elevated level of toll-interacting protein (TOLLIP) in presence of porin. PMID: 25152369
    20. The MUC5B promoter polynmorphism, TOLLIP, is a strong risk factor for idiopathic pulmonary fibrosis in a Mexican population but is very rare in a Korean population. PMID: 25275363
    21. Tollip depletion causes cytotoxicity toward polyQ proteins, whereas Tollip overexpression clears human cells from Huntington's disease-linked polyQ proteins by autophagy; Tollip is a Functional Homolog of Yeast Cue5. PMID: 25042851
    22. negative regulator of pathological cardiac hypertrophy by blocking the AKT signalling pathway PMID: 24285748
    23. Just the individuals with genotype C/C of rs3750920 of the TOLLIP have a trend of protective effect to developing lepromatous leprosy . PMID: 24294608
    24. in the absence of polyubiquitinated cargo, the dual binding of ubiquitin partitions Tollip into membrane-bound and membrane-free states, a function that contributes to the engagement of Tollip in both membrane trafficking and cytosolic pathways. PMID: 23880770
    25. Tollip cooperates with Smad7 to modulate intracellular trafficking and degradation of ubiquitinated TbetaRI, whereby negatively regulates TGF-beta signaling pathway. PMID: 23027871
    26. These data demonstrate that TOLLIP has an anti-inflammatory effect on TLR2 and TLR4 signaling in humans PMID: 22778396
    27. located in the cytoplasm of cytotrophoblasts in the first-trimester placental tissues PMID: 22582869
    28. Our findings indicated that the variants in TOLLIP were significantly associated with sepsis susceptibility in the Chinese Han population. PMID: 21219635
    29. the results indicate that insufficient O-GlcNAc modification prevents Elf-1-mediated transcriptional repression and thereby upregulates Tollip gene expression in intestinal epithelial cells. PMID: 21867680
    30. Data show that knockdown of Tollip reduces CNF1-induced Rac1-dependent UPEC entry. PMID: 21291504
    31. These findings suggest that basic residues of the C2 domain mediate membrane targeting of Tollip by interaction with phosphoinositides, which contribute to the observed partition of the protein in different subcellular compartments. PMID: 21294713
    32. DSCR1-1S isoform positively modulates IL-1R-mediated signaling pathways by regulating Tollip/IRAK-1/TRAF6 complex formation. PMID: 19716405
    33. Here we report that Tollip also associates directly with TLR2 and TLR4 and plays an inhibitory role in TLR-mediated cell activation PMID: 11751856
    34. a Tom1-Tollip complex functions as a factor that links polyubiquitinated proteins to clathrin PMID: 14563850
    35. Tollip and Tom1 form a complex and regulate endosomal trafficking of ubiquitinated proteins PMID: 15047686
    36. following stimulation by exogenous CD26, Tollip and IRAK-1 dissociate from caveolin-1, and IRAK-1 is then phosphorylated in the cytosol, leading to the upregulation of CD86 via activation of NF-kappaB PMID: 16107720
    37. Tollip is required for sorting of IL-1RI at late endosomes. PMID: 17113392
    38. Variation in the TOLLIP gene may play a role in the pathogenesis of AD. PMID: 17362526
    39. Expression of TLR-8, but not Tollip, is highly up-regulated in the colonic epithelium from patients with active IBD PMID: 18985539
    40. Significant & strong 2- & 3-locus interactions between SNPs in TOLLIP (rs4963060), TLR4 (rs6478317) & IRAK1 (rs1059703)were associated with the response to whole-cell vaccine pertussis vaccination in 490 1-yr-old children. PMID: 18987746
    41. Overexpression of Tollip inhibits NF-kappa B activation in response to Toll-like receptor-2 and Toll-like receptor-4 signaling in vitro. PMID: 11441107

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Tollip family
  • 数据库链接：

    HGNC: 16476

    OMIM: 606277

    KEGG: hsa:54472

    STRING: 9606.ENSP00000314733

    UniGene: Hs.368527