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  4. Recombinant Human Thyroid hormone receptor beta (THRB)

Recombinant Human Thyroid hormone receptor beta (THRB)

  • 货号：
    CSB-YP023514HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP023514HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP023514HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP023514HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Avian erythroblastic leukemia viral (v erb a) oncogene homolog 2; C ERBA 2; C ERBA BETA; c-erbA-2; c-erbA-beta; ERBA 2; ERBA BETA; ERBA2; Erythroblastic leukemia viral (v erb a) oncogene homolog 2 avian; generalized resistance to thyroid hormone; GRTH; MGC126109; MGC126110; NR1A2; Nuclear receptor subfamily 1 group A member 2; Oncogene ERBA2; PRTH; THB_HUMAN; THR1; THRB 1; THRB 2; thrB; THRB1; THRB2; Thyroid hormone nuclear receptor beta variant 1; Thyroid hormone receptor beta 1; Thyroid hormone receptor beta 2; Thyroid hormone receptor beta; Thyroid hormone receptor, beta (erythroblastic leukemia viral (v erb a) oncogene homolog 2, avian)
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-461
  • 氨基酸序列
    MTPNSMTENG LTAWDKPKHC PDREHDWKLV GMSEACLHRK SHSERRSTLK NEQSSPHLIQ TTWTSSIFHL DHDDVNDQSV SSAQTFQTEE KKCKGYIPSY LDKDELCVVC GDKATGYHYR CITCEGCKGF FRRTIQKNLH PSYSCKYEGK CVIDKVTRNQ CQECRFKKCI YVGMATDLVL DDSKRLAKRK LIEENREKRR REELQKSIGH KPEPTDEEWE LIKTVTEAHV ATNAQGSHWK QKRKFLPEDI GQAPIVNAPE GGKVDLEAFS HFTKIITPAI TRVVDFAKKL PMFCELPCED QIILLKGCCM EIMSLRAAVR YDPESETLTL NGEMAVTRGQ LKNGGLGVVS DAIFDLGMSL SSFNLDDTEV ALLQAVLLMS SDRPGLACVE RIEKYQDSFL LAFEHYINYR KHHVTHFWPK LLMKVTDLRM IGACHASRFL HMKVECPTEL FPPLFLEVFE D
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

Could you please indicate the concentration, purity and tag of cat# CSB-EP023514HU-50?

A:
Thanks for your inquiry. The concentration is determined during the production. The puirity is over 85%. The tag is N-terminal 6xHis-SUMO-tagged.

靶点详情

  • 功能：
    Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.
  • 基因功能参考文献：
    1. These data indicate that m-TRbeta1 can act as a tumor suppressor in hepatocarcinoma and its role was significantly better than that of TRbeta1. PMID: 29679278
    2. Thyroid hormone receptor beta and NCOA4 regulate terminal erythrocyte differentiation. PMID: 28864529
    3. Report of a novel mutation T273R in the THRB gene of patients exhibiting signs of the syndrome of resistance to thyroid hormone. s propose that the trans-activating function of TRbeta is likely dominantly hindered in these patients. PMID: 28557707
    4. s previously shown that Nuclear Receptor Corepressor 1 (NCoR) and the thyroid hormone receptor beta1 (TRbeta) inhibit tumor invasion. Here they show that these molecules repress VEGF-C and VEGF-D gene transcription in breast cancer cells, reducing lymphatic vessel density and sentinel lymph node invasion in tumor xenografts. PMID: 27806339
    5. The results emphasized the importance of TRB1 in the regulation of HSV-1 replication in differentiated environment with neuronal phenotype. PMID: 26843385
    6. In certain contexts, Rb loss enables TRbeta1-dependent suppression of SKP2 as a safeguard against RB1-deficient tumorigenesis. TRbeta2 counteracts TRbeta1, thus disrupting this safeguard and promoting development of RB1-deficient malignancies. PMID: 28972075
    7. A relatively stable genome in retinoblastoma tumor cells is maintained by TRb1 and TRb2-mediated PTTG1 inhibition, counteracting Rb-deficiency-related genomic instability. PMID: 28242412
    8. the actions of R429Q-TRbeta1 in Resistance to Thyroid Hormone (RTH)-Syndrome most likely reflect the reduced hormone affinity observed for this mutant rather than an alteration in target gene repertoire. PMID: 28257829
    9. The present studies uncovered a novel mechanism by which thyroid hormone receptor beta could function as a tumor suppressor through modulation of TNF alpha-IkappaB alpha-NFkappaB pathway. PMID: 27254276
    10. Novel THRB single nucleotide substitution-C to G in codon 340 in resistance to thyroid hormone syndrome. PMID: 28222413
    11. TRbeta suppressed Runx2 transcriptional activities, thus confirming TRbeta regulation of Runx2 at functional thyroid hormone-response elements. Significantly, these findings indicate that a ratio of the tumor-suppressor TRbeta and tumor-promoting Runx2 may reflect tumor aggression and serve as biomarkers in biopsy tissues. The discovery of this TRbeta-Runx2 signaling supports the emerging role of TRbeta as a tumor supp... PMID: 27253998
    12. results revealed that microRNA 200a inhibits erythroid differentiation by targeting PDCD4 and THRB PMID: 27734462
    13. it was observed that ER stimulated gene expression by interacting with MEIS1 and FOXP3, and ER inhibited gene expression by interacting with THRB and GRHL1. PMID: 27035558
    14. presents 1 pedigree of thyroid hormone resistance syndrome with heterozygous A317T mutation in THRbeta gene in the proband and his mother, which is the first reported mutation in Chinese and provides a comprehensive review of available literature PMID: 27537566
    15. Molecular cloning and detection of TR beta isoform 4 in pituitary cells. PMID: 26513165
    16. The expression of thyroid receptor beta is linked to fertility status. PMID: 26715425
    17. p.H271D mutation associated with resistance thyroid hormone syndrome PMID: 26041374
    18. diagnosis was confirmed by direct THRB sequencing that revealed 2 novel mutations: the heterozygous p.Ala317Ser in subject 1 and the heterozygous p.Arg438Pro in subject 2 PMID: 25738994
    19. Down-regulation of THRbeta correlates with the reduction of all markers of differentiation and is associated with overexpression of some miRNAs supposed to play a role in thyroid tumorigenesis. PMID: 26003825
    20. propose that the mutated C-terminal region of TRbeta1 could function as an "onco-domain" PMID: 25924236
    21. cases suggested that certain RTHbeta mutants such as p.R316C might show very mild syndrome of inappropriate secretion of thyrotropin in spite of having apparent peripheral resistance to thyroid hormone PMID: 25502991
    22. Loss of heterozygosity in THRB and its proximal microsatellite markers may play a role in tumorigenesis and development in invasive breast cancer. PMID: 26350179
    23. Results showed that triple negative breast cancer patients expressed low level of THRB which was associated with poor outcome and enhanced resistance to both docetaxel and doxorubicin treatment. PMID: 25820519
    24. It is demonstrated in this article that the A234T mutation in the THR-beta gene can cause the thyroid hormone resistance syndrome. PMID: 26273722
    25. Its promoter methylation may be involved in the development of gastric cancer. PMID: 25302749
    26. data indicated altered mineral metabolism in adults and children with resistance to thyroid hormone due to mutations in the THRB gene PMID: 25063548
    27. data demonstrate that TR sumoylation is required for activation of the Wnt canonical signaling pathway during preadipocyte proliferation and enhances the PPARgamma signaling that promotes differentiation PMID: 25572392
    28. down-regulated in hepatocellular carcinoma and precancerous peritumoral tissue PMID: 25156012
    29. miR-424 or miR-503 mediate anti-proliferative and anti-invasive actions of thyroid hormone receptor beta PMID: 24796297
    30. CpG methylation is not the major mechanism contributing to decreased THRB expression in ccRCC PMID: 24849932
    31. Coordinated regulation of transcription and alternative splicing by the thyroid hormone receptor.Thyroid hormone receptor stimulates target gene transcription by binding to the thyroid hormone-response element in the presence of triiodothyronine. PMID: 25019984
    32. The clinical and biochemical characteristics of our samples of Mediterranean populations with thyroid hormone resistance were similar to those previously described. Interestingly, in our populations we identified four novel mutations in the TRbeta gene PMID: 24722129
    33. TRb expression in FTC cells decreased cancer cell proliferation, impeded tumor cell migration, and inhibited tumor growth in vivo through downregulation of the AKT-mTOR-p70 S6P signaling pathway and suppression of VEGF expression PMID: 23731250
    34. T3 throid hormone enhanced the recruitment of the TRbeta1/Oct-1 complex on Octamer-transcription factor-1 site within cyclin D1 promoter. PMID: 24121026
    35. ONL001656673 PMID: 20082849
    36. A heterozygous mutation at codon 350 located in exon 9 of the THRB gene was detected in all the affected members of the family. It is important to consider thyroid hormone levels in association with TSH levels to prevent inappropriate treatmen PMID: 24217081
    37. Using several human ROP enhancer/promoter-luciferase reporter constructs, the study found that thyroid hormone receptor beta 2 increased luciferase activities through the 5'-UTR and intron 3-4 region. PMID: 24058409
    38. Alzheimer's disease brain tissues with elevated neuroserpin protein also showed increased expression of THRbeta1 and HuD PMID: 24036060
    39. Results showthat thyroid hormone receptor beta1 (TRbeta1) mRNA expression was significantly reduced in gastric cancer specimens and the methylation of promoter of TRbeta1 gene in gastric cancer tissues was significantly higher than in adjacent normal tissues. PMID: 24434154
    40. SIRT1 stimulates THRB1 activity in a manner that is independent of PGC-1alpha but requires SIRT1 deacetylase activity. PMID: 23922917
    41. Adenovirus E1A 55R functions as a strong co-activator of TR-dependent transcription. PMID: 24136366
    42. A combination of isoform-specific recruitment and tissue-specific expression of these newly identified coregulator candidates serves to customize TR function for different biological purposes in different cell types. PMID: 23558175
    43. Data indicate that 3,5-diiodothyronine T2 activates the thyroid hormone receptor beta1 (TRbeta1). PMID: 23736295
    44. we report a novel mutation in the THRb gene [c.986C>T (p.Thr329Ile)] in a woman and her daughter, who was also a carrier of the same mutation. PMID: 23195042
    45. TR-beta and LXR-alpha competitively up-regulate the human Seladin-1 promoter, sharing the same response element, site A. PMID: 23416078
    46. One SNP 5' of the thyroid hormone receptor-beta gene was associated with BDR in the childhood population and two adult populations (P-value=0.0012- PMID: 22212731
    47. a novel inverse recruitment mechanism in which liganded TRbeta recruits corepressors to inhibit PLA2g2a expression. PMID: 23629656
    48. THRB intronic SNPs can provide useful information on chemoradiotherapy-related severe myelotoxicity in patients with esophageal squamous cell carcinoma PMID: 23136537
    49. We document a congenital disorder of cone function characterized by severely reduced L- and M-cone responses and increased S-cone responses caused by deleterious mutations in the THRbeta2 gene in thyroid resistant patients. PMID: 22551329
    50. THRB mRNA expression in DTC was 90% lower than in normal controls, and this decrease was associated with a higher tumor/lymph node staging. PMID: 23183175

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  • 相关疾病：
    Generalized thyroid hormone resistance (GTHR); Generalized thyroid hormone resistance autosomal recessive (GTHRAR); Selective pituitary thyroid hormone resistance (PRTH)
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Nuclear hormone receptor family, NR1 subfamily
  • 数据库链接：

    HGNC: 11799

    OMIM: 145650

    KEGG: hsa:7068

    STRING: 9606.ENSP00000348827

    UniGene: Hs.187861