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  4. Recombinant Human NAD (+) hydrolase SARM1 (SARM1)

Recombinant Human NAD (+) hydrolase SARM1 (SARM1)

In Stock
  • 货号：
    CSB-BP750971HU
  • 规格：
    ￥3168
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    SARM1
  • Uniprot No.：
  • 别名：
    FLJ36296; KIAA0524; MyD88-5; SAM and ARM-containing protein; SAM domain-containing protein 2; SAMD2; SARM 1; SARM1; SARM1_HUMAN; Sterile alpha and Armadillo repeat protein; sterile alpha and HEAT/Armadillo motif protein; ortholog of Drosophila; sterile alpha and HEAT/Armadillo motifs-containing protein; sterile alpha and TIR motif containing 1; Sterile alpha and TIR motif-containing protein 1; Sterile alpha and TIR motifs-containing protein 1; Sterile alpha motif domain-containing protein 2; Tir-1 homolog
  • 种属：
    Homo sapiens(Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Baculovirus
  • 分子量：
    80.4 kDa
  • 表达区域：
    28-724aa
  • 氨基酸序列
    LAVPGPDGGGGTGPWWAAGGRGPREVSPGAGTEVQDALERALPELQQALSALKQAGGARAVGAGLAEVFQLVEEAWLLPAVGREVAQGLCDAIRLDGGLDLLLRLLQAPELETRVQAARLLEQILVAENRDRVARIGLGVILNLAKEREPVELARSVAGILEHMFKHSEETCQRLVAAGGLDAVLYWCRRTDPALLRHCALALGNCALHGGQAVQRRMVEKRAAEWLFPLAFSKEDELLRLHACLAVAVLATNKEVEREVERSGTLALVEPLVASLDPGRFARCLVDASDTSQGRGPDDLQRLVPLLDSNRLEAQCIGAFYLCAEAAIKSLQGKTKVFSDIGAIQSLKRLVSYSTNGTKSALAKRALRLLGEEVPRPILPSVPSWKEAEVQTWLQQIGFSKYCESFREQQVDGDLLLRLTEEELQTDLGMKSGITRKRFFRELTELKTFANYSTCDRSNLADWLGSLDPRFRQYTYGLVSCGLDRSLLHRVSEQQLLEDCGIHLGVHRARILTAAREMLHSPLPCTGGKPSGDTPDVFISYRRNSGSQLASLLKVHLQLHGFSVFIDVEKLEAGKFEDKLIQSVMGARNFVLVLSPGALDKCMQDHDCKDWVHKEIVTALSCGKNIVPIIDGFEWPEPQVLPEDMQAVLTFNGIKWSHEYQEATIEKIIRFLQGRSSRDSSAGSDTSLEGAAPMGPT
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer，50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The production of this recombinant Human SARM1 protein is just like all recombinant proteins. The process involved transfecting Baculovirus cells with DNA vector containing the template of recombinant DNA. The Baculovirus cells containing the template were then cultured so that they could transcribe and translate the SARM1 protein. N-terminal 10xHis tag and C-terminal Myc tag was used in the process. The purity is 0.85 determined by SDS-PAGE.

    Sterile alpha and toll/interleukin receptor motif-containing protein 1, SARM1, an executor of axonal degeneration, is an autoinhibitory NAD-consuming enzyme, composed of multiple domains. NMN and its analogs, CZ-48 and VMN, are the only known activators, which can release the inhibitory ARM domain from the enzymatic TIR domain. The later discovery that SARM1 promotes Wallerian degeneration suggested the existence of a pathway that might be targeted therapeutically. More recently, SARM1 was found to execute degeneration by hydrolyzing NAD+. Notably, SARM1 knockdown or knockout prevents neuron degeneration in response to a range of insults that lead to peripheral neuropathy, traumatic brain injury, and neurodegenerative disease. To be clarified, the reduction of cellular NAD+ concentration contributes to the disassembly of SARM1's peripheral ring, which allows formation of active NADase domain dimers, thereby further depleting NAD+ to cause an energetic catastrophe and cell death.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    NAD(+) hydrolase, which plays a key role in axonal degeneration following injury by regulating NAD(+) metabolism. Acts as a negative regulator of MYD88- and TRIF-dependent toll-like receptor signaling pathway by promoting Wallerian degeneration, an injury-induced form of programmed subcellular death which involves degeneration of an axon distal to the injury site. Wallerian degeneration is triggered by NAD(+) depletion: in response to injury, SARM1 is activated and catalyzes cleavage of NAD(+) into ADP-D-ribose (ADPR), cyclic ADPR (cADPR) and nicotinamide; NAD(+) cleavage promoting cytoskeletal degradation and axon destruction. Also able to hydrolyze NADP(+), but not other NAD(+)-related molecules. Can activate neuronal cell death in response to stress. Regulates dendritic arborization through the MAPK4-JNK pathway. Involved in innate immune response: inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38.
  • 基因功能参考文献：
    1. we identify a physical interaction between the autoinhibitory N terminus and the TIR domain of SARM1, revealing a previously unrecognized direct connection between these domains that we propose mediates autoinhibition and activation upon injury. PMID: 27671644
    2. Active nerve degeneration requires SARM1 and MAP kinases, including DLK, while the NAD+ synthetic enzyme NMNAT2 prevents degeneration. PMID: 26844829
    3. Data show that sterile alpha- and armadillo-motif-containing protein (SARM) modulates MyD88 protein-mediated Toll-like receptors (TLRs) activation through BB-loop dependent interleukin-1 receptor (TIR) TIR-TIR interactions. PMID: 26592460
    4. These results indicate that association of PINK1 with SARM1 and TRAF6 is an important step for mitophagy. PMID: 23885119
    5. The innate immunity adaptor SARM translocates to the nucleus to stabilize lamins and prevent DNA fragmentation in response to pro-apoptotic signaling. PMID: 23923041
    6. Rapid Wallerian degeneration requires the pro-degenerative molecules SARM1. PMID: 24840802
    7. Data found that the UXT isoforms elicit dual opposing regulatory effects on SARM-induced apoptosis. PMID: 24021647
    8. SARM overexpression caused mitochondrial clustering which has also been observed in several cell death phenomenon. PMID: 23175186
    9. The N-terminal 27 amino acids (S27) of SARM, which is hydrophobic and polybasic, acts as a mitochondria-targeting signal sequence, associating SARM to the mitochondria. The S27 peptide has an inherent ability to bind to lipids and mitochondria. PMID: 22145856
    10. SARM-mediated inhibition may not be exclusively directed at TRIF or MyD88, but that SARM may also directly inhibit MAPK phosphorylation PMID: 20306472
    11. Candidate gene in the onset of hereditary infectious/inflammatory diseases. PMID: 15893701
    12. TIR adaptor SARM is a negative regulator of Toll-like receptor signaling. PMID: 16964262
    13. confirmed the co-localization of retinoschisin with Na/K ATPase and SARM1 in photoreceptors and bipolar cells of retina tissue PMID: 17804407
    14. SARM1 deficiencies may uncover unexpected similarities between the ways in which neurons and immune cells sense and respond to danger. PMID: 18089857

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  • 亚细胞定位：
    Cytoplasm. Cell projection, axon. Cell projection, dendrite. Cell junction, synapse. Mitochondrion.
  • 组织特异性：
    Predominantly expressed in brain, kidney and liver. Expressed at lower level in placenta.
  • 数据库链接：

    HGNC: 17074

    OMIM: 607732

    KEGG: hsa:23098

    STRING: 9606.ENSP00000406738

    UniGene: Hs.743510