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  4. Recombinant Human Sphingosine kinase 1 (SPHK1)

Recombinant Human Sphingosine kinase 1 (SPHK1)

  • 货号：
    CSB-YP022564HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP022564HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP022564HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP022564HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP022564HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    SK 1; SK1; Sphingosine kinase 1; SPHK 1; SPHK; Sphk1; SPHK1_HUMAN; SPK ; SPK 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-384
  • 氨基酸序列
    MDPAGGPRGV LPRPCRVLVL LNPRGGKGKA LQLFRSHVQP LLAEAEISFT LMLTERRNHA RELVRSEELG RWDALVVMSG DGLMHEVVNG LMERPDWETA IQKPLCSLPA GSGNALAASL NHYAGYEQVT NEDLLTNCTL LLCRRLLSPM NLLSLHTASG LRLFSVLSLA WGFIADVDLE SEKYRRLGEM RFTLGTFLRL AALRTYRGRL AYLPVGRVGS KTPASPVVVQ QGPVDAHLVP LEEPVPSHWT VVPDEDFVLV LALLHSHLGS EMFAAPMGRC AAGVMHLFYV RAGVSRAMLL RLFLAMEKGR HMEYECPYLV YVPVVAFRLE PKDGKGVFAV DGELMVSEAV QGQVHPNYFW MVSGCVEPPP SWKPQQMPPP EEPL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis. Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling leading to IL17 secretion. In response to TNF and in parallel to NF-kappa-B activation, negatively regulates RANTES induction through p38 MAPK signaling pathway. Involved in endocytic membrane trafficking induced by sphingosine, recruited to dilate endosomes, also plays a role on later stages of endosomal maturation and membrane fusion independently of its kinase activity. In Purkinje cells, seems to be also involved in the regulation of autophagosome-lysosome fusion upon VEGFA.; Has serine acetyltransferase activity on PTGS2/COX2 in an acetyl-CoA dependent manner. The acetyltransferase activity increases in presence of the kinase substrate, sphingosine. During neuroinflammation, through PTGS2 acetylation, promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia.
  • 基因功能参考文献：
    1. UCMSCs transfected by SPK1 gene potentially offer a novel mode for the treatment of MS. PMID: 29379030
    2. Neuronal SphK1 acetylates COX2 and contributes to pathogenesis in Alzheimer's disease patients and in a transgenic mouse model. PMID: 29662056
    3. siRNA-mediated knockdown of Sphk2 but not SphK1 resulted in a reduction of cargo content in purified exosomes PMID: 29955024
    4. These findings suggested that miR125b may regulate Alzheimer's disease (AD), and neuronal cell growth and apoptosis, via the regulation of inflammatory factors and oxidative stress by SphK1; therefore, miR125b may be involved in the development of AD. PMID: 29901156
    5. these findings suggest that SphK1 may play a pivotal role in HER2-positive breast carcinogenesis PMID: 28968647
    6. s found that elevated levels of pSphK1 were positive correlation with high expression of S1P, which in turn promoted metastasis of TNBC through S1P/S1PR3/Notch signaling pathway. PMID: 29605826
    7. SphK1 may be novel targets for inhibiting lymph node metastasis in esophageal squamous cell carcinoma. PMID: 28364399
    8. Butyrate and bioactive proteolytic form of Wnt-5a regulate colonic epithelial proliferation and spatial development PMID: 27562371
    9. Immunohistochemistry of xenograft tumors showed significant enhancement of caspase-3 cleavage and suppression of Ki67 and phospho-EGFR by the drug combination, but SphK1 downregulation occurred only in MDA-MB-468 tumors, so is unlikely to be integral to treatment efficacy PMID: 28778177
    10. Data show that sphingosine kinase 1 (SPHK1) was significantly upregulated in oral squamous cell carcinoma (OSCC) tissues and low levels of sphingosine-1-phosphate lyase 1 (SGPL1) mRNA correlated with a worse overall survival, and that sphingosine-1-phosphate receptor 2 (S1PR2) is over-expressed in a subset of tumours, which in part mediates sphingosine 1-phosphate (S1P)-induced migration of OSCC cells. PMID: 27160553
    11. SphK1 expression regulates the early stage of colon carcinogenesis and tumor growth, thus inhibition of SphK1 may be an effective strategy for colon cancer chemoprevention. PMID: 28583134
    12. the blockage of SPHK1 activity to attenuate autophagy may be a promising strategy for the prevention and treatment of patocellular carcinoma PMID: 28521610
    13. Results indicate the involvement of sphingosine kinase-1 (SphK1) in the invasiveness of oral squamous cell carcinoma (OSCC) and in unfavorable prognosis. PMID: 29491060
    14. Sphk1 induced NF-kappaB-p65 activation, increased expression of cyclin D1, shortened the cell division cycle, and thus promoted proliferation of breast epithelial cells. PMID: 27811358
    15. Inhibiting SPHK1 using specific siRNAs or the pharmacological inhibitor FTY720 led to potent anti-cancer activity in nasopharyngeal carcinoma cells/ PMID: 27811359
    16. Everolimus sensitizes prostate cancer cells to docetaxel by down-regulation of HIF-1alpha and sphingosine kinase 1 expression. PMID: 27821815
    17. Sphingosine kinase-1 (SK1) plays a crucial role in the metabolic reprogramming of human ovarian cancer cells. PMID: 28258651
    18. SphK1 is involved in maladaptive hypertrophy and we propose that heart failure might be an additional direct target for therapeutic intervention with SphK1 inhibitors. PMID: 28789830
    19. SPHK1 mediated autophagy is different in neurons and neuroblastoma cells PMID: 27467777
    20. Data indicate that the mitotic kinase Polo-like kinase 1 (PLK1) was an important effector of S1P-S1P5 signaling, and a new function of the SphK1-S1P pathway specifically in the control of mitosis in HeLa cells. PMID: 28351953
    21. SphKs/Sphingosine-1-phosphate signaling is critical for the growth and survival of estrogen receptor positive MCF-7 human breast cancer cells. PMID: 28108260
    22. SK1 activation is renoprotective via induction of autophagy in the fibrotic process PMID: 28733250
    23. Sphingosine kinase 1 is involved in papillary thyroid carcinoma development and progression and can serve as a potential biomarker predictive of lymph node metastasis. PMID: 28982849
    24. High SPHK1 expression is associated with hepatocellular carcinoma. PMID: 28206952
    25. we identified sphingosine kinase 1 (SPHK1) as a novel target of miR-506, the expression of which inhibited the SPHK1/Akt/NF-kappaB signaling pathway, which is activated in pancreatic cancer. High SPHK1 expression was significantly associated with poor survival in a large cohort of pancreatic cancer specimens. PMID: 27065335
    26. Inhibition of SPHK1 in human acute myeloid leukemia cells induces MCL1 degradation and caspase-dependent cell death. PMID: 27956387
    27. The analysis suggests that the catalytic function and regulation of SPHK1 and SPHK2 might be dependent on their conformational mobility. (Review) PMID: 27021309
    28. high expression associated with higher levels of sphingosine-1-phosphate, which in turn is associated with lymphatic metastasis in breast cancer PMID: 27621003
    29. our data suggest that SphK1 modulates the expression of EMT-related markers and cell migration by regulating the expression of p-FAK in CRC cells. Thus, SphK1 may play a functional role in mediating the EMT process in CRC. PMID: 28405684
    30. These results highlight the importance of sphingosine and its conversion to sphingosine-1-phosphate by SphK1 in endocytic membrane trafficking. PMID: 28049734
    31. By simulating therapeutic strategies that target multiple nodes of the pathway such as Raf and SphK1, we conclude that combination therapy should be much more effective in blocking VEGF signaling to EKR1/2. The model has important implications for interventions that target signaling pathways in angiogenesis relevant to cancer, vascular diseases, and wound healing. PMID: 28178265
    32. SphK1 is regulated by PDGF-BB in pulmonary artery smooth muscle cells via the transcription factor Egr-1, promoting cell proliferation. PMID: 27099350
    33. Melatonin-treated cells also exhibited an inhibition of the SphK1/S1P axis. Antifibrogenic effect of SphK1 inhibition was confirmed by treatment of LX2 cells with PF543. Abrogation of the lipid signaling pathway by the indole reveals novel molecular pathways that may account for the protective effect of melatonin in liver fibrogenesis. PMID: 27801960
    34. demonstrated the roles played by miR-144-3p and FN1 in mediating the oncogenic function of SphK1, which enhanced the understanding of the etiology of PTC PMID: 27942860
    35. SphK1 is associated with the proliferation and invasiveness of CRC cells and the SphK1 gene may contribute to a novel therapeutic approach against CRC. PMID: 26662312
    36. SPHK1 mRNA is up-regulation in colorectal cancer. PMID: 26937138
    37. Found that upregulation of SphK1 expression in the stiff substrate is dominant in metastatic cancer cells but not in primary cancer cells. These results suggest that alterations in the mechanical environment of the extracellular matrix surrounding the tumor cells actively regulate cellular properties such as secretion, which in turn, may contribute to cancer progression. PMID: 26877098
    38. In conclusion, these data suggested that HuR bound to SphK1 mRNA and played a crucial role in TGF-beta1-induced hepatic stellate cell activation. PMID: 26912347
    39. low oxLDL concentration triggers sprouting angiogenesis that involves ROS-induced activation of the neutral sphingomyelinase-2/sphingosine kinase-1 pathway, and is effectively inhibited by GW4869. PMID: 26855418
    40. SphK1 expression is functionally associated to cellular proliferation, apoptosis, invasion and mitotane sensitivity of adrenocortical carcinoma. PMID: 26673009
    41. Data show that long non-coding RNA HULC promotes tumor angiogenesis in liver cancer through microRNA miR-107/E2F1 transcription factor (E2F1)/sphingosine kinase 1 (SPHK1) signaling. PMID: 26540633
    42. High SPHK1 expression is associated with ovarian cancer. PMID: 26716409
    43. Sphk1 plays an important role in the malignant transformation of breast epithelial cells and modulates breast cancer metastasis through the regulation of Ecadherin expression. PMID: 26842184
    44. SPHK1 is involved in tumor development and progression of cervical cancer PMID: 26311741
    45. The silencing of SphK1 potentiated the apoptotic effects of the green tea polyphenol epigallocatechin-3-O-gallate. PMID: 26264277
    46. increased SK and SPL mRNA expression along with reduced sphingosine 1-phosphate levels were more commonly observed in hepatocellular carcinoma tissues. PMID: 26886371
    47. Hepatitis B virus X protein is able to elevate the expression of SPHK1 in hepatoma cells by upregulating transcription factor AP2 alpha. PMID: 26073327
    48. In summary, this study demonstrates a link between SphK1/sphingosine-1-phosphate and TGF-beta-induced miR-21 in renal tubular epithelial cells. PMID: 26376826
    49. High SPHK1 protein correlated with resistance to cisplatin (IC50) in an independent gastric cancer cell line panel and with survival of patients treated with chemotherapy prior to surgery but not in patients treated with surgery alone. PMID: 26493335
    50. MiR-506 suppresses the angiogenesis of liver cancer by inhibiting SPHK1. PMID: 26549227

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Cell membrane. Endosome membrane; Peripheral membrane protein. Membrane, clathrin-coated pit. Cell junction, synapse.
  • 组织特异性：
    Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. Expressed in brain cortex (at protein level).
  • 数据库链接：

    HGNC: 11240

    OMIM: 603730

    KEGG: hsa:8877

    STRING: 9606.ENSP00000313681

    UniGene: Hs.68061