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  4. Recombinant Human Sorting nexin-27 (SNX27)

Recombinant Human Sorting nexin-27 (SNX27)

  • 货号：
    CSB-YP842704HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP842704HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP842704HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP842704HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP842704HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SNX27
  • Uniprot No.：
  • 别名：
    KIAA0488; Methamphetamine responsive transcript 1; MGC126871; MGC126873; MGC20471; MRT1; MY014; OTTHUMP00000042487; RP11-98D18.12; SNX27; SNX27_HUMAN; Sorting nexin family member 27; Sorting nexin-27
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-541
  • 氨基酸序列
    MADEDGEGIH PSAPHRNGGG GGGGGSGLHC AGNGGGGGGG PRVVRIVKSE SGYGFNVRGQ VSEGGQLRSI NGELYAPLQH VSAVLPGGAA DRAGVRKGDR ILEVNHVNVE GATHKQVVDL IRAGEKELIL TVLSVPPHEA DNLDPSDDSL GQSFYDYTEK QAVPISVPRY KHVEQNGEKF VVYNVYMAGR QLCSKRYREF AILHQNLKRE FANFTFPRLP GKWPFSLSEQ QLDARRRGLE EYLEKVCSIR VIGESDIMQE FLSESDENYN GVSDVELRVA LPDGTTVTVR VKKNSTTDQV YQAIAAKVGM DSTTVNYFAL FEVISHSFVR KLAPNEFPHK LYIQNYTSAV PGTCLTIRKW LFTTEEEILL NDNDLAVTYF FHQAVDDVKK GYIKAEEKSY QLQKLYEQRK MVMYLNMLRT CEGYNEIIFP HCACDSRRKG HVITAISITH FKLHACTEEG QLENQVIAFE WDEMQRWDTD EEGMAFCFEY ARGEKKPRWV KIFTPYFNYM HECFERVFCE LKWRKENIFQ MARSQQRDVA T
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the retrograde transport from endosome to plasma membrane, a trafficking pathway that promotes the recycling of internalized transmembrane proteins. Following internalization, endocytosed transmembrane proteins are delivered to early endosomes and recycled to the plasma membrane instead of being degraded in lysosomes. SNX27 specifically binds and directs sorting of a subset of transmembrane proteins containing a PDZ-binding motif at the C-terminus: following interaction with target transmembrane proteins, associates with the retromer complex, preventing entry into the lysosomal pathway, and promotes retromer-tubule based plasma membrane recycling. SNX27 also binds with the WASH complex. Interacts with membranes containing phosphatidylinositol-3-phosphate (PtdIns(3P)). May participate in establishment of natural killer cell polarity. Recruits CYTIP to early endosomes.
  • 基因功能参考文献：
    1. Biochemistry and microscopy approaches in T cells confirmed SNX27/ZO-2 PDZ-dependent interaction, and demonstrated its role controlling the dynamic localization of ZO-2 at the IS PMID: 28477369
    2. Results show a crucial role of SNX27 in modulating GPR17 levels by means of a post-translational mechanism and highlights the relationship between the trafficking of the receptor through the endomembrane system and oligodendrocyte differentiation; and provide novel evidence of impairment of GPR17 expression and oligodendrocyte maturation in a mouse model of Down syndrome that is characterized by SNX27 down-regulation. PMID: 27270750
    3. The viral oncoprotein E6 interaction with SNX27 can alter the recycling of cargo molecules, one consequence of which is modulation of nutrient availability in HPV transformed tumour cells. PMID: 27649450
    4. This study defines specific acidic amino acid sequences upstream of the PDZ-binding motif required for high-affinity engagement of the human SNX27 PDZ domain. PMID: 27595347
    5. a function of SNX27 in regulating beta-amyloid (Abeta) generation by modulating gamma-secretase activity. PMID: 25437557
    6. PTHR recycles rapidly through at least two pathways, one involving the ASRT complex of actin, SNX27, and retromer and another possibly involving N-ethylmaleimide-sensitive factor. PMID: 27008860
    7. SNX27 inhibits the Wnt regulated transcription activity of TCF/LEF. Our results suggested that SNX27 interacts with Frizzled receptors to regulate the endocytosis and stability of Fzds PMID: 26744382
    8. SNX27 interaction with FAM21 is required for the precise localization of SNX27 at an endosomal subdomain. PMID: 26956659
    9. SNX27 deficiency is now added to the growing list of neurodegenerative disorders associated with retromer dysfunction. PMID: 25894286
    10. The results suggest that SNX27 undergoes dynamic partitioning between different membrane domains during immunological synapse assembly, and underscore the contribution of unique lipid interactions for SNX27 orchestration of cargo trafficking. PMID: 25472716
    11. The double knockdown of SNX17 and SNX27 results in a striking reduction of papillomavirus 16 infection due to interruption of their binding to the L2 viral protein. PMID: 26202251
    12. RNAi-mediated suppression of ANKRD50 phenocopies loss of SNX27-retromer function. PMID: 25278552
    13. VPS26A binding increases the affinity of the SNX27 PDZ domain for PDZ- binding motifs by an order of magnitude, revealing cooperativity in cargo selection. PMID: 25136126
    14. Ras-association domain of sorting Nexin 27 is critical for regulating expression of GIRK potassium channels. PMID: 23536889
    15. Direct interaction of the SNX27 PDZ domain with the retromer subunit VPS26 is necessary and sufficient to prevent lysosomal entry of SNX27 cargo. PMID: 23563491
    16. SNX27 interacts with MRP4 near the plasma membrane and promotes endocytosis of MRP4 and thereby negatively regulates its cell surface expression and transport function. PMID: 22411990
    17. a model by which SNX27 regulates trafficking of beta-Pix to focal adhesions and thereby influences cell motility. PMID: 21926430
    18. show that sorting nexin 27 (SNX27) serves as an essential adaptor protein linking beta2ARs to the retromer tubule PMID: 21602791
    19. These results identify SNX27 as a PDZ-containing component of the T cell immunological synapse, and demonstrate a role for this protein in the regulation of the Ras-ERK pathway, suggesting a functional relationship between SNX27 and DGKzeta. PMID: 21303929
    20. indicate that SNX27 may function to regulate endocytosis and/or endosomal sorting of NR2C PMID: 21300787
    21. Results identify a discrete role of SNX27 in PDZ-directed recycling of a physiologically important signaling receptor, and extend the concept of cargo-specific molecular sorting in the recycling pathway. PMID: 20733053
    22. Overexpression of SNX17 in mammalian cells promotes the endocytosis of P-selectin from the plasma membrane and inhibits its movement into lysosomes. PMID: 15121882
    23. These data support a previously unreported role for diacylglycerol kinase zeta in the modulation of membrane trafficking, which may also help to define SNX27 function. PMID: 17351151
    24. These results suggest that endosomal SNX27 may recruit CASP to orchestrate intracellular trafficking and/or signaling complexes. PMID: 17577583
    25. regulation of Kir3 channels by SNX27 depends critically on the combination of Kir3 subunits PMID: 18690037
    26. This study shows that SNX27 promotes the endosomal movement of Kir3 (or GIRK) channels, leading to reduced surface expression, increased degradation and smaller Kir3 potassium currents. PMID: 17828261
    27. SNX27a redirects part of the 5-HT4(a)R to early endosomes. PMID: 15466885

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  • 亚细胞定位：
    Early endosome membrane; Peripheral membrane protein. Cytoplasm, cytosol. Note=Localizes to immunological synapse in T-cells. In T-cells, recruited from the cytosol to sorting endosomes by phosphoinositide-3-kinase products.
  • 蛋白家族：
    Sorting nexin family
  • 组织特异性：
    Widely expressed. Expressed in cells of hematopoietic origin (at protein level).
  • 数据库链接：

    HGNC: 20073

    OMIM: 611541

    KEGG: hsa:81609

    STRING: 9606.ENSP00000357836

    UniGene: Hs.192326