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  4. Recombinant Human Sororin (CDCA5)

Recombinant Human Sororin (CDCA5)

  • 货号：
    CSB-YP822211HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP822211HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP822211HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP822211HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP822211HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CDCA5
  • Uniprot No.：
  • 别名：
    CDCA5; CDCA5_HUMAN; Cell division cycle associated protein 5; Cell division cycle-associated protein 5; MGC16386; p35; Sororin
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-252
  • 氨基酸序列
    MSGRRTRSGG AAQRSGPRAP SPTKPLRRSQ RKSGSELPSI LPEIWPKTPS AAAVRKPIVL KRIVAHAVEV PAVQSPRRSP RISFFLEKEN EPPGRELTKE DLFKTHSVPA TPTSTPVPNP EAESSSKEGE LDARDLEMSK KVRRSYSRLE TLGSASTSTP GRRSCFGFEG LLGAEDLSGV SPVVCSKLTE VPRVCAKPWA PDMTLPGISP PPEKQKRKKK KMPEILKTEL DEWAAAMNAE FEAAEQFDLL VE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Regulator of sister chromatid cohesion in mitosis stabilizing cohesin complex association with chromatin. May antagonize the action of WAPL which stimulates cohesin dissociation from chromatin. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Required for efficient DNA double-stranded break repair.
  • 基因功能参考文献：
    1. High CDCA5 expression is associated with hepatocellular carcinoma. PMID: 30015982
    2. These results suggest that satellite I RNA plays a role in stabilizing RBMX and Sororin in the ncRNP complex to maintain proper sister chromatid cohesion. PMID: 29383807
    3. Silencing of CDCA5 suppresses proliferation of gastric cancer cells by inducing G1-phase arrest via downregulating CCNE1. PMID: 29326043
    4. Sororin participates in the regulation of centromeric cohesion during meiosis in collaboration with SGO2-PP2A. PMID: 26951638
    5. The results suggest that CDCA5 functions as a critical gene supporting oral squamous cell carcinoma progression and that targeting CDCA5 may be a useful therapeutic strategy for oral squamous cell carcinoma. PMID: 26497678
    6. phosphorylation plays unexpected roles in regulating the subcellular localization of Sororin. PMID: 26177583
    7. the C-terminus of Sororin functions as an anchor binding to SA2, which facilitates other conserved motifs on Sororin to interact with other proteins to regulate sister chromatid cohesion and separation. PMID: 25608232
    8. We propose that the Prp19 complex and the splicing machinery contribute to the establishment of cohesion by promoting Sororin accumulation during S phase, and are, therefore, essential to the maintenance of genome stability. PMID: 25092791
    9. A transcriptome-wide analysis revealed that SNW1 or PRPF8 depletion affects the splicing of specific introns in a subset of pre-mRNAs, including pre-mRNAs encoding the cohesion protein sororin and the APC/C subunit APC2. PMID: 25257309
    10. Targeting p35/Cdk5 signalling via CIP-peptide promotes angiogenesis in hypoxia. PMID: 24098701
    11. Data show that the MEK-ERK pathway regulates cell cycle-related neuronal apoptosis (CRNA) by elevating the levels of cyclin D1, and the increase in cyclin D1 attenuates the activation of cyclin-dependent kinase 5 (cdk5) by its neuronal activator p35. PMID: 22833568
    12. Sororin is phosphorylated by Cdk1/cyclin B at prophase and acts as a docking protein to bring Plk1 into proximity with SA2, resulting in the phosphorylation of SA2 and the removal of cohesin complexes from chromosomal arms. PMID: 21987589
    13. the interaction of the highly conserved motif at the C terminus of sororin with the cohesin complex is critical to its ability to mediate sister chromatid cohesion. PMID: 21115494
    14. Transactivation of CDCA5 and its phosphorylation at Ser209 by ERK play an important role in lung cancer proliferation. PMID: 20551060
    15. data indicate that sororin interacts with chromatin-bound cohesin and functions during the establishment or maintenance of cohesion in S or G2 phase, respectively PMID: 17349791
    16. Sororin protects centromeric cohesion in response to the spindle checkpoint, but prevents the removal of cohesion by a mechanism independent of the anaphase-promoting complex. PMID: 17361102

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  • 亚细胞定位：
    Nucleus. Chromosome. Cytoplasm. Note=Associates with nuclear chromatin from S phase until metaphase and is released in the cytoplasm upon nuclear envelope breakdown.
  • 蛋白家族：
    Sororin family
  • 数据库链接：

    HGNC: 14626

    OMIM: 609374

    KEGG: hsa:113130

    STRING: 9606.ENSP00000275517

    UniGene: Hs.434886