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  4. Recombinant Human Solute carrier family 22 member 6 (SLC22A6), partial

Recombinant Human Solute carrier family 22 member 6 (SLC22A6), partial

  • 货号：
    CSB-YP700197HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP700197HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP700197HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP700197HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP700197HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SLC22A6
  • Uniprot No.：
  • 别名：
    FLJ55736; hOAT1; hPAHT; hROAT1; MGC45260; OAT1; Organic anion transporter 1; OTTHUMP00000236796; OTTHUMP00000236797; OTTHUMP00000236798; OTTHUMP00000236799; PAH transporter; PAHT; Para aminohippurate transporter; Renal organic anion transporter 1; ROAT1; S22A6_HUMAN; SLC22A6; Solute carrier family 22 (organic anion transporter) member 6; Solute carrier family 22 member 6
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the renal elimination of endogenous and exogenous organic anions. Functions as organic anion exchanger when the uptake of one molecule of organic anion is coupled with an efflux of one molecule of endogenous dicarboxylic acid (glutarate, ketoglutarate, etc). Mediates the sodium-independent uptake of 2,3-dimercapto-1-propanesulfonic acid (DMPS). Mediates the sodium-independent uptake of p-aminohippurate (PAH), ochratoxin (OTA), acyclovir (ACV), 3'-azido-3-'deoxythymidine (AZT), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS) and 3-carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF), cidofovir, adefovir, 9-(2-phosphonylmethoxyethyl) guanine (PMEG), 9-(2-phosphonylmethoxyethyl) diaminopurine (PMEDAP) and edaravone sulfate. PAH uptake is inhibited by p-chloromercuribenzenesulphonate (PCMBS), diethyl pyrocarbonate (DEPC), sulindac, diclofenac, carprofen, glutarate and okadaic acid. PAH uptake is inhibited by benzothiazolylcysteine (BTC), S-chlorotrifluoroethylcysteine (CTFC), cysteine S-conjugates S-dichlorovinylcysteine (DCVC), furosemide, steviol, phorbol 12-myristate 13-acetate (PMA), calcium ionophore A23187, benzylpenicillin, furosemide, indomethacin, bumetamide, losartan, probenecid, phenol red, urate, and alpha-ketoglutarate.
  • 基因功能参考文献：
    1. is richly expressed in the kidney, where it plays critical roles in the secretion, from the blood to urine, of clinically important drugs. PMID: 29422382
    2. Our findings suggest that because OAT1 is capable of transporting d-luc, it can also be used to improve visualization and monitoring of luciferase-expressing cells. PMID: 29273507
    3. SLC22A6 variants were not significantly associated with hyperuricemia and gout in a New Zealand Maori and Pacific cohort. PMID: 28371506
    4. this is the first demonstration that Nedd4-1 regulates hOAT1 ubiquitination, expression, and transport activity through its WW2 and WW3 domains PMID: 27226107
    5. both Nedd4-1 and Nedd4-2 are important regulators for hOAT1 ubiquitination, expression, and function PMID: 26823285
    6. Uremic toxins, p-cresyl sulfate and indoxyl sulfate, are transported into endothelial cells by OAT1/OAT3. PMID: 28472795
    7. high capacity p-cresyl sulfate trasnporter PMID: 24185403
    8. OAT1 and NaDC3 in the basolateral membrane and OAT4 in the luminal membrane of proximal tubule cells are responsible for the avid renal secretion of N-carbamoylglutamate. PMID: 25354943
    9. BCL6 constitutes a promising candidate gene for the regulation of human OAT1 transcription PMID: 25234312
    10. Clopidogrel/clopidogrel carboxylate are weak inhibitors of OAT1. PMID: 24530383
    11. The results confirmed that OAT1, OAT3, OCT2, MATE1, and MATE2-K were coexpressed in tubular epithelial cells. PMID: 23630107
    12. transport of xanthurenic acid by OAT1 and OAT3 PMID: 23832370
    13. PKC isoform PKCalpha was responsible for OAT1 ubiquitination. PMID: 23640180
    14. It was shown that human OAT3 and OAT1 cannot be involved in the renal extraction of glutathione from blood, but OAT1 could support intracellular glutathione synthesis by taking up cysteinyl glycine. PMID: 23255614
    15. Both hOAT1 and hOAT3 markedly stimulated the uptake of kynurenic acid into oocytes. PMID: 22108572
    16. High urine OAT1 and OAT3 and low OAT4 is associated with early reversible proximal tubular damage. PMID: 21945944
    17. The data reveal alpha-ketoglutarate as a common high-affinity substrate of NaDC3, OAT1, and OAT3 PMID: 21865262
    18. Intergenic polymorphism rs10792367 between OAT1 and OAT3 is not associated with hypertension, but appears to be involved in between-individual variations in antihypertensive responses to hydrochlorothiazide. PMID: 21164499
    19. Our results are the first to highlight the central role of TM 12 in maintaining the stability and in promoting the maturation efficiency of hOAT1. PMID: 19892921
    20. results suggest that genetic polymorphisms may not be a significant contributing factor to variations in the hOAT2 expression or hOAT2 transport activity PMID: 19854166
    21. elucidation of the molecular mechanism for renal tetracycline transport by human organic anion transporters (hOATs) using proximal tubular cells stably expressing hOATs PMID: 11855680
    22. cimetidine was found to be substrate for fROAT/hOAT1; organic anion transporters likely contribute to cimetidine excretion in proximal tubules PMID: 12429554
    23. hOAT1 transports urate,and is not responsible for familial juvenile gouty nephropathy in the two sisters examined in this study. PMID: 12472777
    24. PKC-induced hOAT1 downregulation is achieved through carrier retrieval from the cell membrane and does not involve phosphorylation of the predicted classic hOAT1 PKC consensus sites PMID: 12874449
    25. stimulation of basolateral organic anion uptake by EGF or PGE2 is a widespread (if not general) regulatory mechanism, and the signal transduction pathway involved seems to be general PMID: 14644751
    26. glycosylation has a role in the targeting of OAT1 onto the plasma membrane PMID: 14749323
    27. The level of human OAT1 mRNA is significantly lower in the kidney of patients with renal diseases than in the normal controls. PMID: 14984259
    28. ...provide evidence for a general expression of the recently identified splice-variants of hOTA1...Their expression is restricted to the kidney cortex. p. 430 PMID: 15039295
    29. Leu-30 and Thr-36 in transmembrane domain 1 are critical determinants of hOAT1 function PMID: 15145940
    30. Organic anion transporter(s) likely play prominent role in basolateral transport of mercuric ions by proximal tubular cells and in nephropathy induced by Hg(2+). PMID: 15200431
    31. mercuric conjugates of Hcy are potential transportable substrates of OAT1. PMID: 15284288
    32. the coding region of OAT1 has low genetic and functional diversity and its variants may not contribute substantially to interindividual differences in renal elimination of xenobiotics PMID: 15864112
    33. hOAT1 has been suggested as the basis of nephrotoxicity induced by nucleoside phosphonate analogs PMID: 15914676
    34. hOAT1 exists in the plasma membrane as a homooligomer, possibly trimer, and higher order of oligomer PMID: 16046403
    35. CH(3)Hg-Cys is transported by hOAT1 PMID: 16164645
    36. The clustering of OAT genes in the genome raises the possibility that nucleotide polymorphisms in SLC22A6 could also effect SLC22A8 expression, and vice versa. PMID: 16648942
    37. the C terminus of hOAT1 has a central role in its function PMID: 16920720
    38. identified two new residues, Tyr(230) and Phe(438), which are important for substrate/protein interactions PMID: 17038320
    39. this paper reports the first characterization of the human OAT1 promoter and the first gene in the kidney whose promoter activity is regulated by HNF-4alpha PMID: 17344191
    40. it is concluded that Arg(466) influences the binding of glutarate, but not interaction with p-aminohippurate, and interacts with chloride, which is a major determinant in substrate translocation PMID: 17353191
    41. Both hOAT1 and hOAT3 are responsible for the basolateral uptake of edaravone sulfate in the kidney. PMID: 17502342
    42. hOAT1 did not mediate rosuvastatin uptake PMID: 17585018
    43. The data provide a model for the concerted action of OAT1 mediating basolateral secretion of glutarate derivatives from proximal tubule cells PMID: 18365245
    44. OAT1 undergoes constitutive and protein kinase C-regulated trafficking through a dynamin- and clathrin-dependent pathway PMID: 18818201
    45. The renal expression of OAT1 and OAT3 should be taken into account in order to improve pharmacotherapeutic efficacy and to prevent drug toxicity during the onset of this hepatic disease. PMID: 19034961
    46. Gaboxadol could be taken up into the kidney by hOAT1 followed by glucuronidation and efflux of the conjugate into urine via MRP4. PMID: 19082692
    47. ANG II inhibited hOAT1 activity through activation of PKCalpha, which led to the redistribution of the transporter from the cell surface to the intracellular compartments. PMID: 19088254
    48. OAT1 appears to be one of the major contributors to renal basolateral uptake of citrulline, and impaired activities of these transporters may contribute substantially to the increase in plasma citrulline in renal failure. PMID: 19403644

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Major facilitator (TC 2.A.1) superfamily, Organic cation transporter (TC 2.A.1.19) family
  • 组织特异性：
    Strongly expressed in kidney and to a lower extent in liver, skeletal muscle, brain and placenta. Found at the basolateral membrane of the proximal tubule.
  • 数据库链接：

    HGNC: 10970

    OMIM: 607582

    KEGG: hsa:9356

    STRING: 9606.ENSP00000367102

    UniGene: Hs.369252