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  4. Recombinant Human Sodium/potassium/calcium exchanger 2 (SLC24A2), partial

Recombinant Human Sodium/potassium/calcium exchanger 2 (SLC24A2), partial

  • 中文名称：
    人SLC24A2重组蛋白
  • 货号：
    CSB-YP883419HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人SLC24A2重组蛋白
  • 货号：
    CSB-EP883419HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人SLC24A2重组蛋白
  • 货号：
    CSB-EP883419HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人SLC24A2重组蛋白
  • 货号：
    CSB-BP883419HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人SLC24A2重组蛋白
  • 货号：
    CSB-MP883419HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SLC24A2
  • Uniprot No.：
  • 别名：
    Na(+)/K(+)/Ca(2+) exchange protein 2; Na(+)/K(+)/Ca(2+)-exchange protein 2; NCKX 2; NCKX2_HUMAN; OTTHUMP00000045115; Retinal cone Na Ca+K exchanger; Retinal cone Na-Ca+K exchanger; Slc24a2; Sodium/potassium/calcium exchanger 2; Solute carrier family 24 (sodium/potassium/calcium exchanger) member 2; Solute carrier family 24 member 2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Critical component of the visual transduction cascade, controlling the calcium concentration of outer segments during light and darkness. Light causes a rapid lowering of cytosolic free calcium in the outer segment of both retinal rod and cone photoreceptors and the light-induced lowering of calcium is caused by extrusion via this protein which plays a key role in the process of light adaptation. Transports 1 Ca(2+) and 1 K(+) in exchange for 4 Na(+).
  • 基因功能参考文献：
    1. a new mutation in SLC24A2 gene was identified to decrease the stability of SLC24A2 PMID: 28413183
    2. Functional and structural properties of the NCKX2 Na(+)-Ca (2+)/K (+) exchanger: a comparison with the NCX1 Na (+)/Ca (2+) exchanger. PMID: 23224872
    3. identified 31 residues that, when substituted, result in shifts in Na(+) affinity, either toward higher or lower K(m) values when compared with wild type NCKX2 (K(m) for Na(+) 58 mm) PMID: 20231282
    4. The oligomeric state of this protein is studied. PMID: 12502543
    5. This is the first systematic NCKX mutagenesis study in which 96 residues are mutated in the human cone NCKX2 cDNA, and functional consequences of these mutations are measured. PMID: 12525183
    6. results appear to rule out a critical importance of an intramolecular disulfide linkage in NCKX2 protein synthesis and folding as had been reported before PMID: 15196038
    7. Glu(188) and Asp(548) are the central residues of a single cation binding pocket that can accommodate both K(+) and Ca(2+). Furthermore, a single set of residues lines a transport pathway for both K(+) and Ca(2+). PMID: 15583008
    8. Charge-removing replacement of Asp(575) by either asparagine or cysteine rendered the mutant NCKX2 proteins independent of K(+), whereas the charge-conservative substitution of Asp(575) to glutamate resulted in a nonfunctional mutant NCKX2 protein PMID: 15611132
    9. Several residues in helix H2 of NCKX2 are in the proximity of residues in helices H8 and to aspartate575 in H9; the residues found here are conserved in NCKX1-5 isoforms. PMID: 15909993
    10. PKC-mediated activation of NCKX2 is sensitive to mutation of multiple PKC consensus sites via a mechanism that may involve several phosphorylation events PMID: 17038313
    11. A regulatory kinetic state of calcium (Ca2+) transport via NCKX2 Na+/Ca2+-K+ exchangers may play a prominent role in regulation of Ca2+ extrusion in cellular environments such as neuronal synapses that experience frequent and dynamic Ca2+ fluxes. PMID: 17164249
    12. non-synonymous single nucleotide polymorphism in SLC24A5 alters a residue that is important for NCKX5 and NCKX2 activity PMID: 18166528

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  • 亚细胞定位：
    Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family, SLC24A subfamily
  • 数据库链接：

    HGNC: 10976

    OMIM: 609838

    KEGG: hsa:25769

    STRING: 9606.ENSP00000344801

    UniGene: Hs.283014