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  4. Recombinant Human Sodium/glucose cotransporter 1 (SLC5A1), partial

Recombinant Human Sodium/glucose cotransporter 1 (SLC5A1), partial

  • 货号：
    CSB-YP021675HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP021675HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP021675HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP021675HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP021675HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    D22S675; High affinity sodium glucose cotransporter 1; High affinity sodium glucose cotransporter; High affinity sodium-glucose cotransporter; Human Na+/glucose cotransporter 1; Na(+)/glucose cotransporter 1; Na+/glucose cotransporter 1; NAGT; SC5A1_HUMAN; SGLT 1; SGLT1; SLC5A1; Sodium glucose cotransporter 1; Sodium/glucose cotransporter 1; Solute carrier family 5 (sodium/glucose cotransporter) member 1; Solute carrier family 5 member 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.
  • 基因功能参考文献：
    1. Only the index variant in SLC5A1 showed a significant association with fasting glucose in the expected opposing direction. Products of genes in all 1,5-AG-associated loci have known roles in carbohydrate digestion and enteral or renal glucose transport, suggesting that genetic variants associated with 1,5-AG influence its concentration via effects on glucose metabolism and handling PMID: 28588231
    2. High glucose levels induce MMP-2 expression in Human cardiac fibroblasts , possibly by up-regulation of SGLT1. PMID: 29512713
    3. Data suggest that a fraction of sodium glucose transporter is rapidly degraded by lysosomes and never reached the plasma membrane; another fraction reaches the membrane and is subsequently degraded by lysosomes following internalization. PMID: 28193781
    4. Data suggest expression of SGLT1 is markedly increased in kidney of patients with type 2 diabetes as compared to control subjects; SGLT1 mRNA is highly and significantly correlated with fasting and postprandial plasma glucose and HbA1c. In contrast, data suggest SGLT2 and GLUT2 mRNA in kidney are down-regulated in type 2 diabetes, but not to statistically significant level. (GLUT2 = glucose transporter type 2) PMID: 28477418
    5. Data suggest that SGLT2 expression is higher in control kidney than in kidney from subjects with type 2 diabetes; SGLT1 expression in kidney tended in the same direction; SGLT2 appears to be localized to tubular brush-border membranes; unaffected renal tissues were obtained from subjects undergoing unilateral nephrectomy for renal carcinomas. PMID: 28419670
    6. Duodenal SGLT-1 expression is increased in individuals with 1-hour postload hyperglycemia or impaired glucose tolerance, as well as in subjects with T2 Diabetes mellitus, and it positively correlates with early postload glucose excursion. PMID: 28938485
    7. Homoeriodictyol affects both the glucose metabolism and the serotonin system in Caco-2 cells via a sodium glucose cotransporter 1 protein (SGLT-1)-meditated pathway. PMID: 28192456
    8. intracellular Na+ and sugar release occurs in a random, independent manner PMID: 27325773
    9. Sodium-Glucose Cotransporter (hSGLT1) Is a Disulfide-Bridged Homodimer with a Re-Entrant C-Terminal Loop PMID: 27137918
    10. JAK3 up-regulates SGLT1 activity by increasing the carrier protein abundance in the cell membrane, an effect enforcing cellular glucose uptake into activated lymphocytes and thus contributing to the immune response. PMID: 27595398
    11. Results found that SGLT1 is required for FLIPL induced cell aerobic glycolysis and survival to low glucose conditions. In patients with hepatocellular carcinoma, SGLT1 expression level was positively correlated with FLIPL expression level. PMID: 27178057
    12. Although the similarity between the pf values of SGLT1 and aquaporin-1 makes a transcellular pathway plausible, it renders water pumping physiologically negligible because the passive flux would be orders of magnitude larger. PMID: 26945065
    13. Elevated SGLT activity increases Na+ influx into myocytes and causes Na+ overload in type 2 diabetes. PMID: 26316524
    14. Compound K induces SGLT1 expression and glucose uptake in differentiated intestinal Caco-2 cells. PMID: 25600494
    15. phlorizin binding domains in sodium-glucose cotransporter family PMID: 26086341
    16. Data demonstrate a role for Per1 in the transcriptional regulation of NHE3 and SGLT1 in the kidney. PMID: 26377793
    17. Cardiac SGLTs, possibly SGLT1 in particular, appear to provide an important protective mechanism against ischemia-reperfusion injury by replenishing ATP stores in ischemic cardiac tissues. PMID: 26121582
    18. CREB activation is essential for EGF-induced SGLT1 gene expression. PMID: 25936754
    19. Gglucose galactose malabsorption...analysis lead to the identification of 2 novel mutations... PMID: 24048166
    20. Delphinidin-3-glucoside protects against oxidized low-density lipoprotein-induced mitochondrial dysfunction in vascular endothelial cells via the sodium-dependent glucose transporter SGLT1. PMID: 23874689
    21. The Na2 site is conserved in hSGLT1, the side chain of S392 and the backbone carbonyl of S393 are important in the first Na+ binding, and that Na+ binding to Na2 promotes binding to Na1 and also sugar binding. PMID: 24191006
    22. Data indicate that IGF-1R and SGLT1 interact in HEK293 and MCF7 cells, and IGF-1R siRNA transfection results in down-regulation of SGLT1. PMID: 23531874
    23. Results suggest that the use of MAP17 and SGLT1 markers may identify patients who are likely to exhibit a better response to treatments that boost oxidative stress in other cancer types. PMID: 23418532
    24. B-RAF upregulates SGLT1 activity, an effect requiring vesicle insertion into the cell membrane. PMID: 23010278
    25. in hSGLT1, pi-pi interaction between outer gate residues F101 and F453 contributes to holding the sugar in the occluded conformation after binding. PMID: 23116249
    26. A seven-state kinetic model is established that describes the activity of SGLT1 up to a 2 ms time resolution. PMID: 23008432
    27. SGLT1 overexpression, as examined by immunohistochemistry, is an independent biomarker for poor prognosis of patients with ovarian carcinoma. PMID: 22159627
    28. Expression of SGLT1 and EGFR in colorectal cancer tissues was higher than that in normal tissues and their expression related with clinical stage. PMID: 21080109
    29. Data suggest that "gate" residues in SGLT1 contribute directly to the coupling between substrate and Na+ transport PMID: 22159082
    30. the structural basis of cotransporter water permeability PMID: 22004742
    31. JAK2 upregulates SGLT1 activity which may play a role in the effect of JAK2 during ischemia and malignancy. PMID: 21406183
    32. An independent estimation of the turnover rate for human SGLT1 expressed in Xenopus laevis oocytes, was obtained applying the ion-trap technique. PMID: 21190656
    33. Glucose-galactose malabsorption is life-threatening newborn diarrhea caused by mutations in the Na(+) /glucose cotransporter gene SLC5A1 that described herein. PMID: 20486940
    34. HPV18 E6 oncoprotein participates in the upregulation of SGLT1. PMID: 21156162
    35. The role of SGLT1 and SGLT2 in renal glucose reabsorption are discussed. PMID: 20980548
    36. The role of SGLT1 and SGLT2 in renal glucose reabsorption, and the potential for targeting these transporters in diabetes there are discussed. PMID: 21048164
    37. This study indicates that the leak current associated with SGLT1 is mediated by a variety of monovalent cations, including cations that do not generate the conformational changes associated to the Na+ binding site used for cotransport. PMID: 20338844
    38. Mutations of this gene protein (mapped to Chromosome 22) causes glucose-galactose malabsorption in infants. Sugar transport is impaired mainly because the mutant proteins are either truncated or are not targeted properly to the cell membrane. PMID: 12139397
    39. Intracellular compartments containing SGLT1 are involved in the regulation of SGLT1 abundance at the apical cell surface. PMID: 12773314
    40. Aspartate residue 454 of SGLT1 is critically important for normal trafficking of the protein to the plasma membrane. PMID: 15476411
    41. Our results indicate that the major voltage-dependent step of the Na(+)/glucose transport cycle is the return of the empty carrier from inward to outward facing conformations. PMID: 15596535
    42. Thus, the C351A and C361A mutations might cause a global reorganization of the disulfide bonds of SGLT1. PMID: 15885653
    43. the large, hydrophilic loop near the carboxyl terminus of SGLT1 does not appear to play a major part in the binding of phlorizin PMID: 15904891
    44. SGLT-1 has a role in glucose uptake and in protecting intestinal epithelial cells against LPS-induced apoptosis and barrier defects PMID: 16260652
    45. 3 conformational states of SGLT1 differ in their packing density and surface hydrophobicity, reflecting the empty carrier, the d-glucose loaded carrier facing the outside of membrane and the complex of the outside-orientated carrier with phlorizin PMID: 16300400
    46. water transport across the membrane can be explained by cotransport of water in the membrane proteins and that intracellular unstirred layers effects are minute PMID: 16322051
    47. results indicate that cysteine residues C255 and C511 form a disulfide bridge in human SGLT1 and that this disulfide bridge is involved in the conformational change of the free carrier PMID: 16446504
    48. hRS1 protein exhibits glucose-dependent, short-term inhibition of hSGLT1 and hOCT2 by inhibiting the release of vesicles from the trans-Golgi network. PMID: 16788146
    49. description of a novel feedback mechanism in apoptotic signaling pathway for SGLT-1-dependent cytoprotection; observation suggests new function for CD14 on enterocytes involving induction of caspase-dependent SGLT-1 activity which leads to cell rescue PMID: 16860318
    50. study examines the conformations of the Na(+)/glucose cotransporter during sugar transport using charge and fluorescence measurements PMID: 17130520

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  • 相关疾病：
    Congenital glucose/galactose malabsorption (GGM)
  • 亚细胞定位：
    Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Sodium:solute symporter (SSF) (TC 2.A.21) family
  • 组织特异性：
    Expressed mainly in intestine and kidney.
  • 数据库链接：

    HGNC: 11036

    OMIM: 182380

    KEGG: hsa:6523

    STRING: 9606.ENSP00000266088

    UniGene: Hs.1964