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  4. Recombinant Human Smoothened homolog (SMO), partial

Recombinant Human Smoothened homolog (SMO), partial

  • 货号：
    CSB-YP857876HU1
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP857876HU1
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP857876HU1-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP857876HU1
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP857876HU1
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    SMO; SMOH; Smoothened homolog; SMO; Protein Gx
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia. Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation.
  • 基因功能参考文献：
    1. Study present the crystal structure of the multi-domain human SMO in complex with a specially designed seven-transmembrane helices domain (TMD) ligand, shedding light on interactions between the cysteine-rich domain and TMD. Combining the structures with biophysical characterization and computer modelling results, study propose a mechanistic model of SMO activation. PMID: 28513578
    2. Case Report: Happle-Tinschert Syndrome is caused by mosaic SMO mutations and represents a variant of Curry-Jones syndrome. PMID: 29335739
    3. cholesterol within the bilayer is sufficient for constitutive Smoothened activation. Cholesterol effects occur independently of the lipid-binding Smoothened extracellular domain, a region that is dispensable for Patched1-Smoothened coupling. PMID: 29229834
    4. A somatic mutation in SMO likely accounts for the structural malformations and predisposition to form bowel hamartomas and myofibromas. The mutation burden in the involved tissues likely accounts for the variable manifestations PMID: 28386950
    5. Dual MET and SMO Inhibitors are potent antiproliferative agents in EGFR-Tyrosine kinase inhibitors resistant human non small cell lung cancer. PMID: 28787156
    6. Smoothened-antagonists reverse alterations in Hedgehog signaling and chondrocyte primary cilium length in homogentisic acid induced model of alkaptonuria. PMID: 28019670
    7. SMO mutation is associated with olfactory groove meningiomas. PMID: 28082415
    8. SMO is covalently modified by cholesterol. This modification is regulated by Ptch1 and Hh and is essential for Hh signaling. PMID: 28344083
    9. A screen for analogs revealed another six molecules, with IC50 values in the low micromolar range. Importantly, one of the most active of the new antagonists continued to be efficacious at the D473H mutant of Smoothened, which confers clinical resistance to the antagonist vismodegib in cancer treatment. PMID: 27490099
    10. Our studies demonstrate an important role for smoothened and glioma-associated oncogene homology-1 in gastric cancer and suggest inhibition of hedgehog pathway as a novel and potent strategy to treat gastric cancer patients PMID: 28675107
    11. The results suggest that Shh signaling plays an important role in rheumatoid arthritis-Fibroblast-like synoviocytes proliferation in a Smo-dependent manner. PMID: 26189371
    12. Mutations in SMO gene is associated with Curry-Jones Syndrome. PMID: 27236920
    13. The Hedgehog pathway receptor SMO is an important regulator of gastric cancer paclitaxel resistance. PMID: 28350784
    14. Role of SMO-mediated Hedgehog signaling pathway in the proliferation and apoptosis of multiple myeloma cells PMID: 27959416
    15. exogenous addition of 3beta,5alpha-dihydroxycholest-7-en-6-one, a naturally occurring B-ring oxysterol derived from 7-DHC that also accumulates in Smith-Lemli-Opitz syndrome, blocked Hedgehog signaling by inhibiting activation of the essential transduction component Smoothened, through a mechanism distinct from Smoothened modulation by other lipids. PMID: 27162362
    16. High SMO expression is associated with pancreatic and skin cancers. PMID: 26784250
    17. crystal structures of the Hh signal transducer and oncoprotein Smoothened, a GPCR that contains two distinct ligand-binding sites: one in its transmembrane domain and one in the cysteine-rich domain PMID: 27437577
    18. Smoothened determines beta-arrestin-mediated removal of the G protein-coupled receptor Gpr161 from the primary cilium. PMID: 27002170
    19. The combined inhibition of SMO and EGFR exerted a strong antiproliferative activity. PMID: 26124204
    20. Suggest that hypoxia promotes SMO transcription through upregulation of MAML3 and RBPJ to induce proliferation, invasiveness and tumorigenesis in pancreatic cancer. PMID: 26655998
    21. These findings suggest that miR-218 inhibits multidrug resistance of gastric cancer cells by down-regulating smoothened expression. PMID: 26261515
    22. Recipient SMO rs3824 polymorphism is associated with an increased risk of hepatocellular carcinoma recurrence following orthotopic liver transplantation. PMID: 25944162
    23. Strikingly, in basal cell carcinoma patients treated with Smo inhibitor, squamous cell cancers with RAS/MAPK activation emerged from the antecedent basal cell carcinoma tumors. PMID: 26130651
    24. report discussed two different SMO mutations representing examples of primary or secondary resistance respectively to vismodegib in two distinct basal cell carcinoma cases PMID: 25306392
    25. The SMOF412E mutation was not detected in ameloblastoma. The BRAFV600E-activating mutation is a common event in ameloblastomas, occurring regardless of site or histological type. This mutation is also detected in odontogenic carcinomas. PMID: 25854168
    26. SMO was upregulated in gliomas and was associated with tumor grade and survival period. SMO inhibition suppressed the biological behaviors of glioma cells. SMO expression was inversely correlated with miR-326 and is a novel direct target of miR-326. PMID: 25173582
    27. SMO expression was significantly associated with better colorectal cancer-specific survival. PMID: 25023548
    28. The Smoothened receptor (SMO) mediates signal transduction in the hedgehog pathway, which is implicated in normal development and carcinogenesis. PMID: 25008467
    29. analysis of small-molecule regulation of SMO and discussion of potential mechanisms for its endogenous regulation [review] PMID: 25785427
    30. SMO mutations are implicated in drug resistance in basal cell carcinoma. PMID: 25759019
    31. Mutations within the SMO protein are implicated in drug resistance in basal cell carcinoma. PMID: 25759020
    32. Studies indicate that Smoothened (Smo) inhibitor was beneficial for the treatment of advanced basal cell carcinoma (BCC). PMID: 24469022
    33. signaling mediated by Smo contributes to acquired chemoresistance through activating the transcriptional factor Gli PMID: 24393163
    34. SMO mutation is asociated with ameloblastoma. PMID: 24859340
    35. MicroRNA-338-3p could inhibit colorectal carcinoma cell invasion and migration by inhibiting smoothened expression. PMID: 24277750
    36. The structure of the Smo receptor will promote the development of small molecules interacting with a key therapeutic target with interests in regenerative medicine and cancer.[review] PMID: 24148123
    37. miR-338-3p could suppress colorectal carcinoma growth by inhibiting SMO protein expression. PMID: 23599646
    38. We observed strong correlation between higher SMO and SHH expression levels with poorer overall survival. PMID: 23379358
    39. Smo antagonism led to reduced Hedgehog pathway activity, resulting in decreased cell proliferation and induction of apoptosis via the intrinsic pathway in the esophageal cancer cells PMID: 23915072
    40. GSA-10 allows the pharmacological characterization of a novel Smo active site. PMID: 23448715
    41. downregulation of miR-326 may be a possible mechanism for unrestricted activation of Smo signal transducer of the oncogenic Hedgehog pathway in chronic myeloid leukemia PMID: 23341351
    42. Nonclassical activation of Hedgehog signaling enhances multidrug resistance and makes cancer cells refractory to Smoothened-targeting Hedgehog inhibition PMID: 23508962
    43. crystal structure of the transmembrane domain of the human SMO receptor bound to the small-molecule antagonist LY2940680 at 2.5 A resolution PMID: 23636324
    44. A subset of meningiomas lacking NF2 alterations harbored recurrent oncogenic mutations in AKT1 (p.Glu17Lys) and SMO (p.Trp535Leu) and exhibited immunohistochemical evidence of activation of these pathways. PMID: 23334667
    45. High SMO expression is asociated with postoperative liver metastasis in colon cancer. PMID: 23098507
    46. SMO mutations, which activate Hedgehog signaling, were identified in ~5% of non-NF2 mutant meningiomas PMID: 23348505
    47. High SMO expression is associated with colorectal cancer. PMID: 22901214
    48. Study shows that hedgehog signaling rewires cellular metabolism; Smo-dependent noncanonical signal rewires metabolism in vitro and in vivo. PMID: 23063129
    49. different pools of Smo move into cilia through distinct mechanisms PMID: 22864913
    50. the effects of oxysterols on Hedgehog signaling are exquisitely stereoselective, consistent with the hypothesis that they function through a specific protein target. PMID: 22231273

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  • 相关疾病：
    Curry-Jones syndrome (CRJS)
  • 亚细胞定位：
    Membrane; Multi-pass membrane protein. Cell projection, cilium.
  • 蛋白家族：
    G-protein coupled receptor Fz/Smo family
  • 数据库链接：

    HGNC: 11119

    OMIM: 601500

    KEGG: hsa:6608

    STRING: 9606.ENSP00000249373

    UniGene: Hs.437846