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  4. Recombinant Human Ski-like protein (SKIL)

Recombinant Human Ski-like protein (SKIL)

  • 货号：
    CSB-YP021357HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP021357HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP021357HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP021357HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP021357HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    OTTHUMP00000213557; OTTHUMP00000213591; OTTHUMP00000213592; OTTHUMP00000213595; SKI like; SKI like oncogene; Ski like protein; SKI like proto oncogene; Ski related oncogene; Ski related oncogene snoN; Ski related protein; Ski-like protein; Ski-related oncogene; Ski-related protein; SKIL; SKIL_HUMAN; SNO; SnoA; SnoI; SnoN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-684
  • 氨基酸序列
    MENLQTNFSL VQGSTKKLNG MGDDGSPPAK KMITDIHANG KTINKVPTVK KEHLDDYGEA PVETDGEHVK RTCTSVPETL HLNPSLKHTL AQFHLSSQSS LGGPAAFSAR HSQESMSPTV FLPLPSPQVL PGPLLIPSDS STELTQTVLE GESISCFQVG GEKRLCLPQV LNSVLREFTL QQINTVCDEL YIYCSRCTSD QLHILKVLGI LPFNAPSCGL ITLTDAQRLC NALLRPRTFP QNGSVLPAKS SLAQLKETGS AFEVEHECLG KCQGLFAPQF YVQPDAPCIQ CLECCGMFAP QTFVMHSHRS PDKRTCHWGF ESAKWHCYLH VNQKYLGTPE EKKLKIILEE MKEKFSMRSG KRNQSKTDAP SGMELQSWYP VIKQEGDHVS QTHSFLHPSY YLYMCDKVVA PNVSLTSAVS QSKELTKTEA SKSISRQSEK AHSSGKLQKT VSYPDVSLEE QEKMDLKTSR ELCSRLDASI SNNSTSKRKS ESATCNLVRD INKVGIGLVA AASSPLLVKD VICEDDKGKI MEEVMRTYLK QQEKLNLILQ KKQQLQMEVK MLSSSKSMKE LTEEQQNLQK ELESLQNEHA QRMEEFYVEQ KDLEKKLEQI MKQKCTCDSN LEKDKEAEYA GQLAELRQRL DHAEADRQEL QDELRQEREA RQKLEMMIKE LKLQILKSSK TAKE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May have regulatory role in cell division or differentiation in response to extracellular signals.
  • 基因功能参考文献：
    1. It is a critical negative regulator of TGF-beta1/Smad signal pathway, involving in tubule epithelial-mesenchymal transition (EMT), extracellular matrix (ECM) accumulation, and tubulointerstitial fibrosis. PMID: 28707079
    2. signal transducer and activator of transcription (Stat)3 represses Smad3 in synergy with the potent negative regulators of TGF-beta signaling, c-Ski and SnoN, whereby renders gefitinib-sensitive HCC827 cells resistant PMID: 28115165
    3. SnoN interacts with multiple components of the Hippo pathway to inhibit the binding of Lats2 to TAZ and the subsequent phosphorylation of TAZ, leading to TAZ stabilization. PMID: 27237790
    4. suggest that SnoN suppresses TGF-betainduced epithelial-mesenchymal transition and invasion of bladder cancer cells in a TIF1gammadependent manner PMID: 27430247
    5. the findings of this study demonstrate that the downregulation of SnoN expression in hRPTECs under high-glucose conditions is mediated by the increased expression of Smurf2 through the TGF-b1/Smad signaling pathway. PMID: 26743567
    6. RNAi-mediated downregulation of SnoN effectively inhibited proliferation and enhanced apoptosis of pancreatic cells. PMID: 25907906
    7. SKIL knockdown led to growth arrest in PC-3 and LNCaP cell line models of prostate cancer, and its overexpression led to increased invasiveness in RWPE-1 cells. PMID: 25749039
    8. Whole exome sequencing of the blood of the patient and both parents revealed a de novo germline SKIL mutation in the child that was not present in either parent PMID: 25464936
    9. Data indicate that tripartite motif containing 33 protein TIF1gamma promotes sumoylation of SKI-like proto-oncogene protein SnoN1 and regulates epithelial-mesenchymal transition (EMT). PMID: 25059663
    10. The results indicate that protein ubiquitination promotes megakaryopoiesis via degrading SnoN, an inhibitor of CD61 expression, strengths the roles of ubiquitination in cellular differentiation. PMID: 24637302
    11. SnoNspecific siRNA is capable of effectively inhibiting the expression of SnoN in human HepG2 cells, and the downregulation of SnoN expression induces growth inhibition and apoptosis PMID: 23446947
    12. these studies identify TLOC1 and SKIL as driver genes at 3q26 and more broadly suggest that cooperating genes may be coamplified in other regions with somatic copy number gain. PMID: 23764425
    13. High SnoN expression is associated with metastasis in breast cancer. PMID: 23832742
    14. Phospholipid Scramblase 1, an interferon-regulated gene located at 3q23, is regulated by SnoN/SkiL in ovarian cancer cells. PMID: 23621864
    15. These data strongly suggest that SnoN can function as a tumor suppressor at early stages of tumorigenesis in human cancer tissues. PMID: 23418461
    16. These results support our observation that cancer tissues have lower expression levels of SnoN, miR-720, and miR-1274A compared to adjacent normal tissues from esophageal squamous cell carcinoma patients. PMID: 23154181
    17. Data suggest that SKIL expression is modulated by antineoplastic agents and may be involved in drug resistance in ovarian carcinoma; up-regulation of SKIL expression by arsenic trioxide and reduction of apoptosis involves activation of PI3K pathway. PMID: 23178716
    18. SNON predominantly associates with SMAD2 at the promoters of primitive streak (PS) and early DE marker genes PMID: 23154981
    19. the SNON-SMAD4 complex negatively regulated basal SKIL gene expression through binding the promoter and recruiting histone deacetylases PMID: 22674574
    20. SnoN mediates a negative feedback mechanism evoked by TGF-beta to inhibit BMP signaling and, subsequently, hypertrophic maturation of chondrocytes. PMID: 22767605
    21. SnoN may have broad functions in the embryonic development and tissue morphogenesis [Review] PMID: 22710172
    22. analysis of SnoN signaling in proliferating cells and postmitotic neurons [review] PMID: 22710173
    23. SnoN level promotes ERalpha signaling and possibly breast cancer progression. PMID: 22227247
    24. The flexibility in the putative protein binding groove enables SnoN to recognize multiple interaction partners. PMID: 20957027
    25. Regulation of TGF-beta-co-repressor (SnoN) is greatly affected suggesting that SnoN as a cardinal player in cholestasis-induced fibrogenesis. PMID: 19889106
    26. The endogenous SnoN plays a role in regulating ADAM12 expression in response to TGFbeta1. PMID: 20457602
    27. SnoN elevation is associated with mammary gland branching morphogenesis, postlactational involution, and mammary tumorigenesis. PMID: 20460516
    28. BMP-7 prevents TGF-beta-mediated loss of the transcriptional repressor SnoN and hence specifically limits Smad3 DNA binding. PMID: 20093492
    29. Inhibition of Smad signaling may be achieved at the transcriptional level through c-Ski/receptor-Smad/co-mediator Smad4 interactions--REVIEW PMID: 19898560
    30. SnoN acts as a positive mediator of TGF-beta-induced transcription and cell cycle arrest in lung epithelial cells PMID: 15677458
    31. Sno expression was identified as an important mechanism to shut off antiproliferative TGF-beta signaling in malignant melanoma PMID: 15809735
    32. mechanism of regulation of TGF-beta signaling via differential subcellular localization of SnoN PMID: 16109768
    33. A novel role of SnoN in the transforming activity of TGF-beta in fibroblasts was demonstrated. PMID: 16314499
    34. SnoN also seems to regulate negatively the TGF-beta-responsive SMAD, mothers against DPP homolog 7 gene by binding and repressing its promoter in a similar way to Ski PMID: 16442497
    35. SnoN is directly regulated by sumoylation leading to the enhancement of the ability of SnoN to repress transcription in a promoter-specific manner PMID: 16966324
    36. snoN protein has both oncogenic and tumor suppressive properties in colorectal tumorigenesis. PMID: 17062133
    37. SnoN plays both pro-tumorigenic and antitumorigenic roles at different stages of mammalian malignant progression PMID: 17074815
    38. Arkadia induces degradation of SnoN and c-Ski in addition to Smad7. PMID: 17510063
    39. Results show that Arkadia specifically activates transcription via Smad3/Smad4 binding sites by inducing degradation of the transcriptional repressor SnoN. PMID: 17591695
    40. CREB activation, in concert with Sp1, constitutes a molecular switch that confers the cell type-specific induction of SnoN in response to HGF stimulation PMID: 17625116
    41. SnoN overexpression is associated with depth of invasion and recurrence in patients with esophageal squamous cell carcinoma. PMID: 18612694
    42. Data show that dominant-negative transforming growth factor beta type II receptor decreases matrix metalloproteinase 2 in hepatic stellate cells, and upregulates SKI-like oncogene, which antagonizes TGF-beta signaling. PMID: 19189315
    43. results implicate SnoN levels in multiple roles during ovarian carcinogenesis: promoting cellular proliferation in ovarian cancer cells and as a positive mediator of cell cycle arrest and senescence in non-transformed ovarian epithelial cells PMID: 19383336
    44. SnoN is involved in differentiation in normal skin and benign and nonmetastatic skin tumors, but plays a proto-oncogenic role in undifferentiated squamous cell carcinoma PMID: 19538364

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  • 蛋白家族：
    SKI family
  • 组织特异性：
    Isoform SNON and isoform SNOA are widely expressed. Highest expression is found in skeletal muscle, followed by placenta and lung. Lowest expression in heart, brain and pancreas. Isoform SNOI expression is restricted to skeletal muscle.
  • 数据库链接：

    HGNC: 10897

    OMIM: 165340

    KEGG: hsa:6498

    STRING: 9606.ENSP00000259119

    UniGene: Hs.536655