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  4. Recombinant Human Serum paraoxonase/lactonase 3 (PON3)

Recombinant Human Serum paraoxonase/lactonase 3 (PON3)

  • 货号：
    CSB-YP614883HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP614883HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP614883HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP614883HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP614883HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PON3
  • Uniprot No.：
  • 别名：
    2810004E20; AI786302; MGC95026; Paraoxonase 3; Pon3; PON3_HUMAN; Serum paraoxonase/lactonase 3
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-354
  • 氨基酸序列
    GKLVALVLL GVGLSLVGEM FLAFRERVNA SREVEPVEPE NCHLIEELES GSEDIDILPS GLAFISSGLK YPGMPNFAPD EPGKIFLMDL NEQNPRAQAL EISGGFDKEL FNPHGISIFI DKDNTVYLYV VNHPHMKSTV EIFKFEEQQR SLVYLKTIKH ELLKSVNDIV VLGPEQFYAT RDHYFTNSLL SFFEMILDLR WTYVLFYSPR EVKVVAKGFC SANGITVSAD QKYVYVADVA AKNIHIMEKH DNWDLTQLKV IQLGTLVDNL TVDPATGDIL AGCHPNPMKL LNYNPEDPPG SEVLRIQNVL SEKPRVSTVY ANNGSVLQGT SVASVYHGKI LIGTVFHKTL YCEL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Has low activity towards the organophosphate paraxon and aromatic carboxylic acid esters. Rapidly hydrolyzes lactones such as statin prodrugs (e.g. lovastatin). Hydrolyzes aromatic lactones and 5- or 6-member ring lactones with aliphatic substituents but not simple lactones or those with polar substituents.
  • 基因功能参考文献：
    1. PON3 suppressed cell proliferation in vivo and in vitro, which was attributed to its cell-cycle arrest effect. PMID: 27661119
    2. PON3 protein can be detected in plasma and resides in the high-density lipoprotein fraction and protects against Oxidative stress by hydrolyzing certain oxidized lipids in lipoproteins, macrophages and Atherosclerotic lesions. PMID: 29308836
    3. Although inappropriate promoter methylation was not invariantly associated with reduced transcript expression, a significant association was apparent for the ARHGEF4, PON3, STAT5a, and VAX2 gene transcripts (P<0.05). Herein, we present the first genome-wide DNA methylation analysis in a unique HG-NMIBC cohort, showing extensive and discrete methylation changes relative to normal bladder and low-intermediate-grade tumor PMID: 26929985
    4. Low PON3 expression is associated with hepatocellular carcinoma progression. PMID: 27553024
    5. This study showed that the PI3K/Akt pathway is up-regulated by the expression of PON3 in oral squamous cell carcinoma by AP-1. PMID: 27923688
    6. PON3 SNP rs13226149 was associated with intracerebral hemorrhage in log-additive and dominant models. The A allele of rs13226149 contributed to the decreased risk of ICH. SNP rs1053275 was not associated with ICH. PMID: 26227792
    7. Interaction of PON-3 and PON-1 and HDL are associated with the presence and severity of coronary artery disease in type 2 diabetes mellitus patients. PMID: 25964115
    8. PON3 in HDL may be an important protein in preventing atherosclerosis. PMID: 25723336
    9. PON3 is upregulated in cancer tissues and protects against mitochondrial superoxide-mediated cell death. PMID: 22441669
    10. Our results suggest that low serum paraoxonase activity is a risk factor for Alzheimer disease. Furthermore, multiple variants in PON influence serum paraoxonase activity and their effects may be synergistic. PMID: 20980077
    11. Increased serum paraoxonase-3 concentration is associated with insulin sensitivity in peripheral artery disease and with inflammation in coronary artery disease. PMID: 22153698
    12. This study reports for the first time an important increase in serum PON3 concentrations in HIV-infected patients that is associated with their oxidative status and their treatment with NNRTI. PMID: 22003209
    13. investigation of whether gene silencing of Pon3 causes oxidative stress in cell line: Data suggest that PON3 knockdown reduces cell proliferation and total antioxidant capacity at ambient oxygen levels. PMID: 21952037
    14. Serum PON3 concentrations showed a moderate influence (about 10% variation) by PON3 promoter polymorphisms. PMID: 21335322
    15. Paraoxonase-3, a putative circulating antioxidant, is systemically up-regulated in late gestation in the fetal rat, sheep, and human PMID: 20463093
    16. Decreased coronary heart disease risk associated with polymorphisms of paraoxonase 1. PMID: 12151850
    17. Organophosphates are hydrolyzed almost exclusively by PON1, lovastatin and spironolactone are hydrolyzed only by PON3. All three proteins hydrolyze and thereby inactivate N-acyl-homoserine lactones, which are quorum-sensing signals of pathogenic bacteria. PMID: 15772423
    18. The refolded recombinant PON3 exhibited similar antioxidant activity to that of PON3 purified from the soluble fraction of cell lysate and could effectively protect LDL from Cu2+ induced oxidation. PMID: 16139510
    19. Elevated human paraoxanase 3 protein expression significantly decreases atherosclerotic lesion formation and adiposity in male transgenic mice. PMID: 17379834
    20. The rates of hydrolysis of estrogen esters by the paraoxonases is PON3 > PON1 > PON2; diesters are better substrates for the PONs and are very efficiently hydrolyzed, particularly by PON3. PMID: 17412306
    21. Report protective effects of transgene expressed human PON3 against CCl4-induced subacute liver injury in mice. PMID: 19345057
    22. The directed evolution and characterization of recombinant variants of serum paraoxonase PON3, is described. PMID: 19492856

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  • 亚细胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Paraoxonase family
  • 数据库链接：

    HGNC: 9206

    OMIM: 602720

    KEGG: hsa:5446

    STRING: 9606.ENSP00000265627

    UniGene: Hs.440967