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  4. Recombinant Human Serpin H1 (SERPINH1)

Recombinant Human Serpin H1 (SERPINH1)

  • 货号：
    CSB-YP021087HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP021087HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP021087HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP021087HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SERPINH1
  • Uniprot No.：
  • 别名：
    47 kDa heat shock protein; 47 kDa heat shock protein precursor; Arsenic transactivated protein 3; Arsenic-transactivated protein 3; AsTP 3; AsTP3; CBP 1; CBP 2; CBP1; CBP2; Cell proliferation-inducing gene 14 protein; Collagen binding protein 1; Collagen binding protein 2; Collagen binding protein; Collagen-binding protein 2; Collagen-binding protein; Colligen; Colligin 1; Colligin 2; Colligin; colligin-1; colligin-2; gp46; Heat shock protein 47; Heat-shock protein 47; HGNC 1547; Hsp 47; J6; OI10; PIG 14; PIG14; PPROM; Proliferation inducing gene 14; Proliferation inducing gene 14 protein; RA A47; RA-A47; Rheumatoid arthritis antigen A 47; rheumatoid arthritis antigen A-47; Rheumatoid arthritis related antigen RA A47; Rheumatoid arthritis-related antigen RA-A47; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1); serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2); Serine or cysteine proteinase inhibitor clade H member 1; Serine or cysteine proteinase inhibitor clade H member 2; SERPH_HUMAN; Serpin H1; Serpin peptidase inhibitor clade H member 1; serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1); Serpin peptidase inhibitor, clade H, member 1; SERPINH1; SERPINH2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    19-418
  • 氨基酸序列
    AE VKKPAAAAAP GTAEKLSPKA ATLAERSAGL AFSLYQAMAK DQAVENILVS PVVVASSLGL VSLGGKATTA SQAKAVLSAE QLRDEEVHAG LGELLRSLSN STARNVTWKL GSRLYGPSSV SFADDFVRSS KQHYNCEHSK INFRDKRSAL QSINEWAAQT TDGKLPEVTK DVERTDGALL VNAMFFKPHW DEKFHHKMVD NRGFMVTRSY TVGVMMMHRT GLYNYYDDEK EKLQIVEMPL AHKLSSLIIL MPHHVEPLER LEKLLTKEQL KIWMGKMQKK AVAISLPKGV VEVTHDLQKH LAGLGLTEAI DKNKADLSRM SGKKDLYLAS VFHATAFELD TDGNPFDQDI YGREELRSPK LFYADHPFIF LVRDTQSGSL LFIGRLVRPK GDKMRDEL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
  • 基因功能参考文献：
    1. Our present data suggest that HSP47 is an important prognostic factor and an attractive therapeutic target in laryngeal squamous cell carcinoma (LSCC)due to its influence on the biological behaviour of LSCC cells PMID: 28849239
    2. an endoplasmic reticulum complex of resident chaperones that includes HSP47, FKBP65, and BiP regulating the activity of LH2. PMID: 28177155
    3. These observations indicate that this system is appropriate for detecting the interaction between HSP47 and collagen, and could be applied to high-throughput screening for drugs capable of suppressing and/or curing fibrosis. PMID: 29438711
    4. miR-29b can reduce collagen biosynthesis during skin wound healing likely via post-transcriptional inhibition of HSP47 expression. PMID: 27477081
    5. HSP47 expression in patients with colorectal cancer and the number of HSP47-positive spindle cells in the tumor stroma were significantly higher compared with those in adjacent normal colonic mucosa, and the number of the latter cells increased with tumor progression. PMID: 27925182
    6. The essential parts of the Golgi stress response from the perspective of the organelle autoregulation. The pathways of the mammalian Golgi stress response have been identified, specifically the HSP47 pathway. PMID: 28179603
    7. Overexpression of LOXL2 and SERPINH1 was observed in clinical specimens of lung cancer and fibrotic lesions. Downregulation of miR-29a caused overexpression of LOXL2 and SERPINH1 in lung cancer and IPF, suggesting that these genes are involved in the pathogenesis of these two diseases. PMID: 27488440
    8. The changes in the SERPINH1 and SERPINF1 genes in patients with osteogenesis imperfect were synonymous polymorphisms or missense changes located in non-coding regions. PMID: 27706701
    9. Overexpression of HSP47 is associated with poor prognosis in patients with esophageal squamous cell carcinoma and this is consistent with the function of HSP47 in terms of increased cell proliferation and colony formation. PMID: 25953518
    10. A novel homozygous variant in SERPINH1 associated with a severe, lethal presentation of osteogenesis imperfecta with hydranencephaly. PMID: 27677223
    11. The present study demonstrates that HSP47 promotes glioma angiogenesis and highlights the importance of HSP47 as an attractive therapeutic target of GBM. PMID: 25758142
    12. Although the chemical chaperone 4-PBA partially restores the solubility of the Hsp47 OI mutants, collagen-binding activity of Hsp47 was not improved. PMID: 26692483
    13. Data show that the expression of heat shock protein 47 (HSP47) was increased in the peripheral blood mononuclear cells and plasma from scleroderma patients. PMID: 26091621
    14. Mutations in the HSP47 and FKBP65 produce a moderately severe form of Osteogenesis imperfect. PMID: 25510505
    15. In patients with schistosomiasis japonica, TGF-beta1 participates not only in the inflammatory process, but also in the fibrotic process in which Hsp47 and CTGF probably play a key role. PMID: 25111595
    16. Hsp47 expression promotes cancer progression in part by enhancing deposition of extracellular matrix proteins. PMID: 25744716
    17. IL-17A-induced HSP47 expression is involved in collagen I expression in intestinal subepithelial myofibroblasts, which might contribute to intestinal fibrosis in Crohn's disease. PMID: 24534724
    18. silencing of the HSP47 gene significantly inhibited cell migration and invasion in cancer cells and the expression of HSP47 was upregulated in cancer tissues and cervical intraepithelial neoplasia, as demonstrated by immunostaining. PMID: 24141696
    19. miR-29b down-regulates HSP47 and LOX expression. PMID: 24650661
    20. HSP47 is a novel glioma-associated antigen PMID: 24623841
    21. TRAIL induced HSF1 inactivation leads to the suppression of Hsp47-dependent collagen production in activated human hepatic stellate cells. PMID: 23587601
    22. Correlative Hsp47 expression in fibroblasts with bFGF in inflammatory cells may contribute to stromal fibrosis and obstruction in colorectal carcinoma PMID: 23265436
    23. NMR and mutational identification of the collagen-binding site of the chaperone Hsp47 PMID: 23049894
    24. Hsp47 may be related to the TGF-beta1-induced transdifferentiation of human Tenon's fibroblasts to myofibroblasts. PMID: 22967132
    25. Hsp47 recognizes the triple-helix form of procollagen in vitro and in vivo. PMID: 22235129
    26. Overexpression of HSP47 decreased the secretion of heterotrimers containing the mutant collagen alpha5(IV) chain. PMID: 21187648
    27. HSP47 and fascin expression may play role in the pathogenesis of invasive ductal carcinoma of the breast and prostatic adenocarcinoma because their expression is significantly higher than their normal counterpart. PMID: 20701077
    28. We conclude that colligin 2 is expressed in all cellular components of glioma blood vessels and may serve as a general marker for active angiogenesis PMID: 19067716
    29. down-regulated KLF4, CHGA, GPX3, SST and LIPF, together with up-regulated SERPINH1, THY1 and INHBA is an 8-gene signature for gastric cancer PMID: 20043075
    30. Increased heat shock protein 47 expression is associated with esophageal squamous cell carcinoma. PMID: 20112500
    31. The enhancement of HSP47 expression by TGF-beta and IL-1 beta has been confirmed in embryonic lung fibroblasts. PMID: 11994473
    32. induced in cicatricial pemphigoid: possible role(s) in dermal fibrosis PMID: 12061838
    33. Results indicate a novel means by which type I collagen production is regulated by the endoplasmic reticulum constituent, Hsp47. PMID: 12163502
    34. Modulates the production of the endostatin precursor collagen XVIII in head and neck carcinomas PMID: 12174873
    35. levels of HSP47 protein and autoantibodies to HSP47 in the sera of patients with rheumatic autoimmune diseases PMID: 12659832
    36. gene expression profiling in epidermolysis bullosa acquisita PMID: 12824005
    37. high levels of expression of Hsp47 and adult and oncofetal fibronectin in Dupuytren's contracture suggests that cell-mediated alterations in the extracellular environment may play an important role in the disease process PMID: 15047128
    38. HSP47 has a role in aging and photoaging in human fibroblasts PMID: 15247019
    39. The surface-exposed RA-A47 may induce autoantibodies and inflammatory reactions in autoimmune disease situations such as rheumatoid arthritis. PMID: 15389525
    40. HSP47 is constitutively expressed in human hepatic stellate cells and may be a target for antifibrotic therapy. PMID: 15806139
    41. Our results suggest the existence of different fibrotic pathways among these groups involved in the expression of HSP47 and type I procollagen. PMID: 15955241
    42. results suggest nitric oxide has dual effects on collagen synthesis by fibroblasts: the direct stimulation of collagen synthesis due to the up-regulation of procollagen alphaI(1) mRNA, and an indirect effect through the increase of HSP47 mRNA expression PMID: 16171977
    43. analysis of the client recognition mechanism of HSP47 PMID: 16326708
    44. analysis of recognition of the collagen triple helix by chaperone HSP47 PMID: 16484215
    45. A functional SNP in the promoter of the SERPINH1 gene increases risk of preterm premature rupture of membranes in African Americans. PMID: 16938879
    46. The anti-fibrotic effect of pirfenidone may be mediated through direct inhibition of collagen type I expression and inhibition of HSP47 expression in lung fibroblasts. PMID: 18093617
    47. HSP47-positive fibroblasts were main constituent cell of dermatofibroma. PMID: 18095990
    48. These studies define a new haplotype in the SERPINH1 gene that modifies risk of an adverse obstetrical outcome. PMID: 18205191
    49. During keratin preparation from cultured human tumor cell lines, Hsps might be associated with keratin expression in tumor cells PMID: 18293509
    50. Hsp47 was exposed on surface of GPVI-activated platelets; inhibition of Hsp47 abolished platelet aggregation in response to collagen, but partially reduced aggregation in response to other agonists; propose Hsp47 may play a role in hemostasis & thrombosis PMID: 19341245

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  • 相关疾病：
    Osteogenesis imperfecta 10 (OI10)
  • 亚细胞定位：
    Endoplasmic reticulum lumen.
  • 蛋白家族：
    Serpin family
  • 数据库链接：

    HGNC: 1546

    OMIM: 600943

    KEGG: hsa:871

    STRING: 9606.ENSP00000350894

    UniGene: Hs.596449