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  4. Recombinant Human Secretin (SCT)

Recombinant Human Secretin (SCT)

  • 货号：
    CSB-YP020864HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP020864HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP020864HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP020864HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP020864HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SCT
  • Uniprot No.：
  • 别名：
    SCT; Secr; SECR_HUMAN; Secretin; Secretin precursor
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Cytoplasmic domain
  • 表达区域：
    28-54
  • 氨基酸序列
    HSD GTFTSELSRL REGARLQRLL QGLV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Hormone involved in different processes, such as regulation of the pH of the duodenal content, food intake and water homeostasis. Exerts its biological effects by binding to secretin receptor (SCTR), a G-protein coupled receptor expressed in the basolateral domain of several cells. Acts as a key gastrointestinal hormone by regulating the pH of the duodenal content. Secreted by S cells of the duodenum in the crypts of Lieberkuehn and regulates the pH of the duodenum by (1) inhibiting the secretion of gastric acid from the parietal cells of the stomach and (2) stimulating the production of bicarbonate (NaHCO(3)) from the ductal cells of the pancreas. Production of bicarbonate is essential to neutralize the pH and ensure no damage is done to the small intestine by the gastric acid. In addition to regulating the pH of the duodenal content, plays a central role in diet induced thermogenesis: acts as a non-sympathetic brown fat (BAT) activator mediating prandial thermogenesis, which consequentially induces satiation (Probable). Mechanistically, secretin released by the gut after a meal binds to secretin receptor (SCTR) in brown adipocytes, activating brown fat thermogenesis by stimulating lipolysis, which is sensed in the brain and promotes satiation. Also able to stimulate lipolysis in white adipocytes. Also plays an important role in cellular osmoregulation: released into the systemic circulation in response to hyperosmolality and acts at different levels in the hypothalamus, pituitary and kidney to regulate water homeostasis. Also plays a role in the central nervous system, possibly by acting as a neuropeptide hormone: required for hippocampal synaptic function and neural progenitor cells maintenance.
  • 基因功能参考文献：
    1. CD36 is a major mediator of FA-induced release of CCK and secretin. These peptides contribute to the role of CD36 in fat absorption and to its pleiotropic metabolic effects. PMID: 23233532
    2. Data suggest that the interaction between secretin peptide residue H1 and secretin receptor residue W274 is most compatible with a hydrophobic interaction. PMID: 22964305
    3. Secretin effect on increase in transcription of the tyrosine hydroxylase (Th) gene and modulate catecholamine secretion, was tested. PMID: 21597914
    4. binding and activity of a series of 11 truncated and lactam-constrained secretin(5-27) analogues at the prototypic member of this family, the secretin receptor PMID: 21851058
    5. secretin significantly inhibited the tumor size and more than doubled tumor latency, which was associated with a decrease in proliferating cell nuclear antigen and an increase in cleaved-caspase 3 expression levels PMID: 19904746
    6. The purpose of this study was to investigate whether secretin exhibits similar properties in vitro by forming micelles in aqueous solution and interacting with phospholipids PMID: 11814635
    7. Human VPAC1 receptor selectivity filter; Identification of a critical domain for restricting binding PMID: 12133828
    8. fine structure in 11p15.5 and sequence variation in patients with autism PMID: 12160732
    9. human secretin gene is controlled by the Sp1/Sp3 transcription factors ratio and the methylation status of the promoter PMID: 15118068
    10. Transgenic mouse study suggests a trophic role for secretin on neurons known to be involved in multiple superior functions in the normal brain, and lost in neurodegenerative disorders. PMID: 15610158
    11. Secretin mRNAs are found in Purkinje cells and secretin-immunoreactivities are localized in both the soma and dendrites of Purkinje cells of human cerebellum. PMID: 15706223
    12. small heterodimer partner and secretin are potentially co-expressed and lead us to propose a novel regulatory pathway PMID: 16888194
    13. first pathway is by changing the expression levels of nuclear factor-I C(NFI-C) and secretin proteins while the second pathway is by modifying the phosphorylation status of both NFI-C and secretin proteins via cyclin-dependent kinase 1 PMID: 16888198
    14. Peptic ulceration may be a hormonal (secfretin) deficiency disease. PMID: 18280672
    15. These results collectively suggest that variable of tandem repeats could potentially be a functional regulator to control the expression of the human secretin gene in different individuals. PMID: 18566919
    16. secretin acts through stimulation of presynaptic cholinergic neurons in a vagally mediated pathway. PMID: 19239625
    17. Bile acids inhibit duodenal secretin expression via orphan nuclear receptor small heterodimer partner (SHP). PMID: 19372104

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Glucagon family
  • 数据库链接：

    HGNC: 10607

    OMIM: 182099

    KEGG: hsa:6343

    STRING: 9606.ENSP00000176195

    UniGene: Hs.632324