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  4. Recombinant Human Ribonuclease pancreatic (RNASE1)

Recombinant Human Ribonuclease pancreatic (RNASE1)

  • 中文名称：
    人RNASE1重组蛋白
  • 货号：
    CSB-YP019789HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人RNASE1重组蛋白
  • 货号：
    CSB-EP019789HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人RNASE1重组蛋白
  • 货号：
    CSB-EP019789HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人RNASE1重组蛋白
  • 货号：
    CSB-BP019789HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人RNASE1重组蛋白
  • 货号：
    CSB-MP019789HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RNASE1
  • Uniprot No.：
  • 别名：
    HP-RNase; Pancreatic ribonuclease; Rib 1; RIB-1; Rib1; Ribonuclease 1; Ribonuclease A; Ribonuclease a family 1; Ribonuclease pancreatic; Ribonuclease RNase A family 1; Ribonuclease RNase A family 1 pancreatic; RNAS1_HUMAN; RNase 1; RNase A; RNase UpI-1; Rnase1; RNAseA
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    29-156
  • 氨基酸序列
    KE SRAKKFQRQH MDSDSSPSSS STYCNQMMRR RNMTQGRCKP VNTFVHEPLV DVQNVCFQEK VTCKNGQGNC YKSNSSMHIT DCRLTNGSRY PNCAYRTSPK ERHIIVACEG SPYVPVHFDA SVEDST
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA.
  • 基因功能参考文献：
    1. Data suggest that ribonuclease inhibitor (RNH1) protects HeLa cells from ribonuclease 1 (RNase 1). PMID: 27806571
    2. Focusing on the increase in an N-glycosylated Asn residue of serum pancreatic ribonuclease 1, specifically Asn(88), affords a new diagnostic marker for pancreatic cancer PMID: 25336120
    3. Deletion of five residues in the hinge loop of pancreatic ribonuclease induces formation of a domain-swapped dimer that leads to the generation of linear aggregates of pancreatic RNase, revealed by the crystal packing. PMID: 24100329
    4. The reduced conformational flexibility of eosinophil cationic protein can be dynamically and functionally reproduced in the RNase A scaffold. PMID: 23135272
    5. The nuclear transport of PE5 is critical for its cytotoxicity. PMID: 20352290
    6. The role on the dimerization process of different residues of a domain-swapped dimer human pancreatic ribonuclease variant. PMID: 21767499
    7. In human pancreatic ribonuclease both glutamine 28 and arginine 39 are important for the cleavage of dsRNA. PMID: 21408145
    8. Vascular RNase1 and RNase5 are mainly produced by vascular endothelial cells and can serve, depending on the vascular bed, different functions in vascular homeostasis and endothelial cell responses. PMID: 21103661
    9. Studies illustrate of the making dimeric pancreatic RNase through removal by directed mutagenesis of most of the N-terminal alpha-helix to the remainder of the protein. PMID: 19156888
    10. Human endothelial cells selectively express large amounts of pancreatic-type ribonuclease (RNase 1) PMID: 12210760
    11. Results show that Glycine 38 is crucial for the full catalytic activity of the human enzyme on duplex RNA as its substitution with aspartate or alanine results in a drastic reduction in the dsRNA cleavage activity of HPR. PMID: 12237131
    12. Results reveal the dendritic cell-activating activity of pancreatic ribonuclease and suggest that it is a likely participant of inflammatory and immune responses--an endogenous multifunctional immune alarmin. PMID: 15528350
    13. Altogether the results suggest that the pressure-folding transition state of ribonuclease A looks like a collapsed globule with some secondary structure and a weakened hydrophobic core. This is the first direct comparison using a set of mutants. PMID: 16597833
    14. RNase-1 has ribonuclease H activity. PMID: 16738129
    15. Human pancreatic-ribonuclease interacts with importin alpha through different basic residues, including Lys1 and the arginine clusters 31-33 and 89-91. PMID: 16780873
    16. The results were confirmed at the level of mRNA and protein, and suggested that four genes (OPCML, RNASE1, YES1 and ACK1) could play a key role in the tumorigenesis and metastasis of gastric cancer. PMID: 17109515
    17. Structural and energetic aspects of the interaction between human RI (hRI) and human pancreatic ribonuclease (RNase 1), is reported. PMID: 17350650
    18. Coulombic forces mediate extracellular and intracellular equilibria in a dichotomous manner that both endangers cells and defends them from the potentially lethal enzymatic activity of ribonucleases. PMID: 17705507
    19. irrespective of differences in ethnic groups, RNASE1 might show markedly low heterogeneity in its genetic structure with regard to these SNPs PMID: 18219569
    20. Multiple side chain conformations observed for key surface residues are proposed to be crucial for membrane binding as well as translocation and efficient RNA hydrolysis. PMID: 18495155

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Pancreatic ribonuclease family
  • 组织特异性：
    Pancreas and other tissues and body fluids (indicating it may have other physiological functions besides its role in digestion).
  • 数据库链接：

    HGNC: 10044

    OMIM: 180440

    KEGG: hsa:6035

    STRING: 9606.ENSP00000344193

    UniGene: Hs.78224