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  4. Recombinant Human Rho-related GTP-binding protein RhoC (RHOC)

Recombinant Human Rho-related GTP-binding protein RhoC (RHOC)

  • 货号：
    CSB-YP019687HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP019687HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP019687HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP019687HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP019687HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RHOC
  • Uniprot No.：
  • 别名：
    Aplysia RAS-related homolog 9; ARH 9; ARH9; ARHC; H9; MGC1448; MGC61427; Oncogene RHO H9; Ras homolog gene family member C; RAS related homolog 9; RHO C; Rho cDNA clone 9; Rho related GTP binding protein RhoC; Rho-related GTP-binding protein RhoC; rhoC; RhoC GTPase; RHOC_HUMAN; RHOH 9; RHOH9; Small GTP binding protein RhoC
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-190
  • 氨基酸序列
    MAAIRKKLVI VGDGACGKTC LLIVFSKDQF PEVYVPTVFE NYIADIEVDG KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSIDSPD SLENIPEKWT PEVKHFCPNV PIILVGNKKD LRQDEHTRRE LAKMKQEPVR SEEGRDMANR ISAFGYLECS AKTKEGVREV FEMATRAGLQ VRKNKRRRGC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Regulates apical junction formation in bronchial epithelial cells.
  • 基因功能参考文献：
    1. LncRNA TDRG1 overexpression induced tumor development and RhoC expression. PMID: 28984384
    2. Studied the involvement in epithelial ovarian cancer (EOC) of lncRNA ABHD11-AS1 in and the regulation of EOC by RhoC. PMID: 28818073
    3. YMO1 suppresses tumor invasion and metastasis by inhibiting RhoC activity. PMID: 27487132
    4. this studies show the ability of miR-10b to activate the expression of c-Jun through RhoC and NF1, which represents a novel pathway for promoting migration and invasion of human cancer cells PMID: 27494896
    5. swapping residue 188 identity effectively flips the membrane binding profile of wild-type RhoA and RhoC through positive arginine contribution rather than negative phosphoserine regulation PMID: 27355867
    6. HOTAIR is regulated by the RhoC-MRTF-A-SRF signaling pathway in breast cancer cells. PMID: 28069441
    7. E2F transcription factor 1 (E2F1) downregulated micrRNA miR-519d directly and miR-519d downregulated Ras homolog gene family member C (RhoC) directly. PMID: 28146423
    8. During the process of epithelial-mesenchymal transition in A549 cells induced by TGF-beta1, upregulated RhoC protein and RhoC activity were detected, which was associated with the enhanced invasive capability of the cells in vitro. PMID: 27748883
    9. RhoC downregulation may inhibit the proliferation, drug resistance, invasion and migration of ovarian cancer stem cells. PMID: 27748937
    10. Downregulation of RHOC inhibited cholangiocellular carcinoma cells invasion and migration partially via inhibition of matrix metalloproteinase 2, 3 and 9 expression. RHOC also modulated the expression of several epithelial-mesenchymal transition (EMT)-associated proteins PMID: 27108649
    11. RT-PCR and Western blot assays demonstrated that miR-372 transfection reduced the expression of RhoC. PMID: 26673619
    12. study has identified several new proteins like RHOC, DLG5, UGDH, TMOD3 in addition to known chemoresistance associated proteins in non-small cell lung carcinoma. PMID: 26898345
    13. the oncogene RhoC, a driver of metastatic potential, modulates glutamine and N-acetylaspartate metabolism in Inflammatory Breast Cancer cells in vitro, revealing a novel role for RhoC as a regulator of tumor cell metabolism that extends beyond its well known role in cytoskeletal rearrangement. PMID: 27129239
    14. The knockdown of RhoC protein decreased the proliferation rate of the parental and the IE1-expressing glioblastoma cells. PMID: 26741994
    15. found that miR-10b induced HCC cell invasion and migration by modulating the HOXD10 target gene RhoC, uPAR, MMP-2 and MMP-9 expression PMID: 25236186
    16. Smart particles encapsulating the scrambled siRNA sequence did not affect RhoC protein expression. PMID: 26020100
    17. Depletion of the kinases ROCK1 and ROCK2, two known RhoC downstream effectors, similarly decreases cancer interaction with ECs PMID: 25677806
    18. Results identify RhoC as a prominent binding partner of RhoGDI2 and show that RhoGDI2 modulates RhoC activity in cells promoting lung colonization of bladder cancer cells. PMID: 25516960
    19. Inhibition of ovarian epithelial carcinoma tumorigenesis and progression by miR106b proceeds through the RhoC pathway. PMID: 25933027
    20. MiR-93-5P may inhibit EOC tumorigenesis and progression by targeting RhoC. PMID: 25649143
    21. miR-509 has a critical role in brain metastasis of breast cancer by modulating the RhoC-TNF-alpha network. PMID: 25659578
    22. RhoC protein may upregulate VEGF expression, thereby promoting tumor angiogenesis in esophageal squamous cell carcinoma. PMID: 25624724
    23. This is the first report in HNSCC demonstrating the role RhoC plays in mediating EGF-stimulated migration and invasion by down-regulating the PI3K-Akt pathway and E-cadherin expression. PMID: 25622907
    24. may contribute to an epithelial-mesenchymal transition and drug resistance in human breast cancer during chemotherapy PMID: 25123151
    25. RhoC expression might be involved in epithelial-to-mesenchymal transition of ovarian epithelial carcinoma cells. PMID: 24986540
    26. Down-regulation of RhoC expression resulted in decreased migration of neural stem cells. PMID: 24414340
    27. RhoC has a role in regulating cancer stem cells in head and neck squamous cell carcinoma through the over expression of IL-6 and phosphorylation of STAT3 PMID: 24533098
    28. The S1P2R specifically activates RhoC via G12/13 proteins and LARG. PMID: 23993968
    29. RhoC/ROCK1 signaling pathways are likely involved in the progression of cervical squamous cell carcinoma PMID: 24457551
    30. RhoC facilitates tumor cell invasion and promotes subsequent metastasis, in part, by enhancing integrin alpha5beta1 trafficking PMID: 24312560
    31. The up-regulated RhoC expression may affect ovarian carcinogenesis and should be considered a good biomarker for the differentiation and progression of ovarian carcinoma PMID: 23764197
    32. Increased RHOC expression was detected in endometriotic lesions compared to the eutopic endometrium of women with endometriosis and control women. PMID: 23302395
    33. HBx and HBs of hepatitis B virus could increase expression of Ets-1, which consequently contributed to the upregulation of RhoC. PMID: 23474984
    34. This study demonstrated the role of RhoC overexpression in malignant transformation of normal human hepatocytes, suggesting that RhoC may function as an oncogene in hepatocytes. PMID: 23382905
    35. data suggest that RhoC is a key regulator of cell growth and apoptosis in human hepatocellular carcinoma cells PMID: 21674277
    36. RhoC stimulates the proliferation of gastric cancer cells through recruiting IQGAP1 as an effector. PMID: 23145020
    37. MRK is a novel RhoC effector that controls LPA-stimulated cell invasion at least in part by regulating myosin dynamics, ERK and p38 PMID: 23319595
    38. Increased prostate RhoC expression predicted a good outcome after radical prostatectomy for high-grade prostate cancer. PMID: 22935975
    39. RhoC impacts the metastatic potential and abundance of breast cancer stem cells. PMID: 22911725
    40. Substitution of RhoC Ile 43 with a Val increased guanine nucleotide exchange factors-catalyzed exchange in vitro. PMID: 22673745
    41. Knockdown of Hop caused a decrease in the level of RhoC GTPase, and significantly inhibited pseudopodia formation in Hs578T cells. Our data suggest that Hop regulates directional cell migration by multiple unknown mechanisms. PMID: 23036489
    42. Data demonstrate that PKN3 physically interacts with Rho-family GTPases, and preferentially with RhoC, a known mediator of tumor invasion and metastasis in epithelial cancers. PMID: 22217540
    43. Suggest that miR-10b can stimulate the upregulation of RhoC and AKT phosphorylation through targeting HOXD10, thus promoting cell invasion in gastric tumors. PMID: 22293682
    44. miR-493 is a new tumor suppressor miRNA in bladder cancer and inhibits cell motility through downregulation of RhoC and FZD4. PMID: 22057916
    45. In a signaling cascade, the loss of E-cadherin activates the transcriptional regulator ETS-1 and consequently leads to the induction of RhoC expression that stabilizes c-Jun in melanoma. PMID: 21732343
    46. Expression levels of RhoC and ROCK-1 in prostate carcinoma are higher than those in corresponding paracancerous tissues, showing a significant positive correlation with distant metastasis. PMID: 21575520
    47. p38gamma mitogen-activated protein kinase regulates breast cancer cell motility and metastasis, in part, by controlling expression of the metastasis-associated small GTPase RhoC. PMID: 21862636
    48. RHOC expression was induced by direct p53 binding to a regulatory element within the RHOC gene. PMID: 21079653
    49. The knockdown of RhoC expression had no effect on the migration-stimulatory activity of IQGAP1. PMID: 21537845
    50. RhoC promotes polarized tumor cell migration through FMNL3 by restricting lamellipodial broadening. PMID: 21576392

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor; Cytoplasmic side. Cleavage furrow. Note=Translocates to the equatorial region before furrow formation in a ECT2-dependent manner.
  • 蛋白家族：
    Small GTPase superfamily, Rho family
  • 数据库链接：

    HGNC: 669

    OMIM: 165380

    KEGG: hsa:389

    STRING: 9606.ENSP00000285735

    UniGene: Hs.502659