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  4. Recombinant Human RecQ-mediated genome instability protein 1 (RMI1)

Recombinant Human RecQ-mediated genome instability protein 1 (RMI1)

  • 中文名称：
    人RMI1重组蛋白
  • 货号：
    CSB-YP875699HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人RMI1重组蛋白
  • 货号：
    CSB-EP875699HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人RMI1重组蛋白
  • 货号：
    CSB-EP875699HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人RMI1重组蛋白
  • 货号：
    CSB-BP875699HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人RMI1重组蛋白
  • 货号：
    CSB-MP875699HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RMI1
  • Uniprot No.：
  • 别名：
    BLAP75; BLM associated polypeptide 75 kDa; BLM associated protein 75 kDa; BLM-associated protein of 75 kDa; C9orf76; FAAP75; FLJ12888; Homolog of yeast RecQ mediated genome instability 1; RecQ mediated genome instability 1; RecQ-mediated genome instability protein 1; Rmi1; RMI1 homolog; RMI1 RecQ mediated genome instability 1 homolog; RMI1_HUMAN; RP11-346I8
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-625
  • 氨基酸序列
    MNVTSIALRA ETWLLAAWHV KVPPMWLEAC INWIQEENNN VNLSQAQMNK QVFEQWLLTD LRDLEHPLLP DGILEIPKGE LNGFYALQIN SLVDVSQPAY SQIQKLRGKN TTNDLVTAEA QVTPKPWEAK PSRMLMLQLT DGIVQIQGME YQPIPILHSD LPPGTKILIY GNISFRLGVL LLKPENVKVL GGEVDALLEE YAQEKVLARL IGEPDLVVSV IPNNSNENIP RVTDVLDPAL GPSDEELLAS LDENDELTAN NDTSSERCFT TGSSSNTIPT RQSSFEPEFV ISPRPKEEPS NLSIHVMDGE LDDFSLEEAL LLEETVQKEQ METKELQPLT FNRNADRSIE RFSHNPNTTN NFSLTCKNGN NNWSEKNVSE QMTNEDKSFG CPSVRDQNRS IFSVHCNVPL AHDFTNKEKN LETDNKIKQT SSSDSHSLNN KILNREVVNY VQKRNSQISN ENDCNLQSCS LRSSENSINL SIAMDLYSPP FVYLSVLMAS KPKEVTTVKV KAFIVTLTGN LSSSGGIWSI TAKVSDGTAY LDVDFVDEIL TSLIGFSVPE MKQSKKDPLQ YQKFLEGLQK CQRDLIDLCC LMTISFNPSL SKAMVLALQD VNMEHLENLK KRLNK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. Required for BLM phosphorylation during mitosis. Within the BLM complex, required for BLM and TOP3A stability.
  • 基因功能参考文献：
    1. RMI1 knockdown cells exhibit accumulation of broken DNAs after being released from hydroxyurea treatment. Moreover, we demonstrate that RMI1 facilitates the recovery from activated checkpoint and resuming the cell cycle after replicative stress. PMID: 29042194
    2. RMI1 protein level does not change through G1, S and G2 phases, but significantly increases in M phase. PMID: 26556339
    3. a model that maps all functionally important structural features for yeast Rmi1 and structure-prediction-based alignment with the recently established crystal structure of the N-terminus of human Rmi1, is proposed. PMID: 26717309
    4. The results show that Topo IIIalpha stimulates DNA unwinding by BLM in a manner that is potentiated by RMI1-RMI2, and that the processivity of resection is reliant on the Topo IIIalpha-RMI1-RMI2 complex. PMID: 25200081
    5. two proteins that interact with BLM, RMI1 and RMI2, are phosphorylated upon SAC activation, and, like BLM, RMI1, and RMI2, are phosphorylated in an MPS1-dependent manner. PMID: 24108125
    6. RMI1 is required to promote normal replication fork progression PMID: 22645306
    7. Crystal structures of RMI1 and RMI2, two OB-fold regulatory subunits of the BLM complex PMID: 20826342
    8. human topoisomerase IIIalpha functions as a decatenase with the assistance of BLM and RMI1 to facilitate the processing of homologous recombination intermediates without crossing over as a mechanism to preserve genome integrity PMID: 20445207
    9. No evidence was found for an association between RMI1 S455N (rs1982151) and colorectal cancer risk. PMID: 19945966
    10. BLAP75/RMI1 acts by recruiting TOPO IIIalpha to double Holliday junctions PMID: 16537486
    11. BLM, Topo IIIalpha, and BLAP75 constitute a dissolvasome complex that processes HR intermediates to limit DNA crossover formation PMID: 16595695
    12. BLM is stably associated with RMI1 protein. PMID: 17728255
    13. Genetic variant of the human homologous recombination-associated gene RMI1 is associated with acute myeloid leukemia, myelodysplatic syndromes and malignant melanoma PMID: 17900800
    14. evolutionarily conserved N-terminal third of BLAP75 mediates complex formation with BLM and Topo IIIalpha and that the DNA binding activity resides in the C-terminal third of this novel protein. PMID: 18390547
    15. Individuals carrying genetic variants of the BLM-TOP3A-RMI1 complex have an increased risk of acute myeloid leukemia/myelodysplatic syndromes, malignant melanoma, bladder and breast cancer. PMID: 19432957

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  • 亚细胞定位：
    Nucleus. Note=Forms foci in response to DNA damage.
  • 蛋白家族：
    RMI1 family
  • 数据库链接：

    HGNC: 25764

    OMIM: 610404

    KEGG: hsa:80010

    STRING: 9606.ENSP00000317039

    UniGene: Hs.726327