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  4. Recombinant Human Ras-related protein Rab-3A (RAB3A)

Recombinant Human Ras-related protein Rab-3A (RAB3A)

  • 货号：
    CSB-YP019194HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP019194HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP019194HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP019194HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP019194HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Rab 3A; RAB 3A member RAS oncogene family; Rab3a; RAB3A member RAS oncogene family; RAB3A_HUMAN; RAS associated protein RAB 3A; RAS associated protein RAB3A; Ras related protein Rab 3A; Ras related protein Rab3A; Ras-related protein Rab-3A
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-220
  • 氨基酸序列
    MASATDSRYG QKESSDQNFD YMFKILIIGN SSVGKTSFLF RYADDSFTPA FVSTVGIDFK VKTIYRNDKR IKLQIWDTAG QERYRTITTA YYRGAMGFIL MYDITNEESF NAVQDWSTQI KTYSWDNAQV LLVGNKCDME DERVVSSERG RQLADHLGFE FFEASAKDNI NVKQTFERLV DVICEKMSES LDTADPAVTG AKQGPQLSDQ QVPPHQDCAC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Small GTP-binding protein that plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane. Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane. Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed. Stimulates insulin secretion through interaction with RIMS2 or RPH3AL effectors in pancreatic beta cells. Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9. Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1. Plays also a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT. Interacts with MADD (via uDENN domain); the GTP-bound form is preferred for interaction.
  • 基因功能参考文献：
    1. Rab3A-22A blocks exocytosis at a stage downstream intra-acrosomal calcium release. PMID: 27613869
    2. Rab3a accelerates cell proliferation by increasing cyclin D1 expression, enhances anti-cancer drug resistance, and increases tumorigenicity and self-renewal of glioma cells. PMID: 24965146
    3. Data indicate that exocytic stimuli promote ADP ribosylation factor 6 (ARF6) activation, which accomplishes exocytosis by stimulating Rab3A GTP-Binding Protein. PMID: 25713146
    4. Five compounds which possess good inhibitory activity and may act as potential high affinity inhibitors against Rab3A active site were identified PMID: 24652202
    5. alpha-Synuclein membrane association is regulated by the Rab3a recycling machinery and presynaptic activity PMID: 23344955
    6. Rab27 and Rab3 sequentially regulate human sperm dense-core granule exocytosis. PMID: 22753498
    7. Myo5a and Rab3A are direct binding partners and interact on synaptic vesicles and the Myo5a/Rab3A complex is involved in transport of neuronal vesicles PMID: 21349835
    8. a possible role in glomerulopathies PMID: 12937130
    9. Mutation screening of the RAB3A gene in 47 individuals with autism provided no evidence that DNA variants in this gene are associated with autism. PMID: 15005721
    10. Cholesterol content regulates acrosomal exocytosis by enhancing Rab3A plasma membrane association. PMID: 16099449
    11. In conclusion, we have found no evidence for RAB3A conferring susceptibility on mental retardation in the Han Chinese population. PMID: 16584842
    12. Initiates exocytosis in acrosome when prenylated and activated with guanosine triphosphate (GTP). PMID: 17625073
    13. Rab3GEP has a role as the non-redundant guanine nucleotide exchange factor for Rab27a in melanocytes PMID: 18559336
    14. Epac activates the small G proteins Rap1 and Rab3A to achieve exocytosis PMID: 19546222
    15. The maturation of amyloid precursor protein (APP) transport vesicles, including recruitment of conventional kinesin, requires Rab3A GTPase activity. PMID: 19923287

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  • 亚细胞定位：
    Cytoplasm, cytosol. Lysosome. Cytoplasmic vesicle, secretory vesicle. Cell projection, axon. Cell membrane; Lipid-anchor; Cytoplasmic side.
  • 蛋白家族：
    Small GTPase superfamily, Rab family
  • 组织特异性：
    Specifically expressed in brain.
  • 数据库链接：

    HGNC: 9777

    OMIM: 179490

    KEGG: hsa:5864

    STRING: 9606.ENSP00000222256

    UniGene: Hs.27744