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  4. Recombinant Human Ras-related protein Rab-11A (RAB11A)

Recombinant Human Ras-related protein Rab-11A (RAB11A)

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  • 货号：
    CSB-EP019153HU
  • 规格：
    ￥1344
  • 促销：
    促销单品低至599元起
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    MGC1490; Rab 11; Rab 11A; RAB 11A, member oncogene family; Rab-11; RAB11 A; RAB11; Rab11a; RAB11A member RAS oncogene family; Ras related protein Rab 11A; Ras related protein Rab11A; Ras-related protein Rab-11A; RB11A_HUMAN; YL 8; YL8
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    39.9kDa
  • 表达区域：
    2-213aa
  • 氨基酸序列
    GTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNGLSFIETSALDSTNVEAAFQTILTEIYRIVSQKQMSDRRENDMSPSNNVVPIHVPPTTENKPKVQCC
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The small Rab GTPase RAB11A regulates endocytic recycling. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. May also play a role in melanosome transport and release from melanocytes.
  • 基因功能参考文献：
    1. irregularly coated vesicle formation is observed only in influenza A virus infected cells and requires an active Rab11 GTPase. PMID: 29123131
    2. Study suggestes that Rab11a serves as an important oncoprotein and a regulator of YAP in human non-small cell lung cancer. PMID: 28468127
    3. This review will focus on the structure and function of the novel Rab11-FIP3-Rabin8 dual effector complex and its implications for the targeting of sensory receptors to primary cilia, dysfunction of which causes cilia defects underlying human diseases and disorders know as ciliopathies. PMID: 26399276
    4. Data suggest that membrane tethering mediated by endosomal RAB11A is drastically and selectively stimulated by its cognate Rab effectors, class V myosins (MYO5A and MYO5B), in a GTP-dependent manner. (RAB11A = ras-related GTPase Rab-11A; MYO5 = myosin class V) PMID: 28939769
    5. these studies are not only the first to identify alpha4 helix of Rab11a as a protein binding domain on the GTPase but also reveal novel mechanistic insights into the intracellular trafficking of the hIP, and potentially of other members of the GPCR superfamily, involving Rab11-dependent mechanisms PMID: 28739266
    6. transient expression of ATG16L1 not only inhibited autophagosome biogenesis, but also aberrantly targeted RAB11-positive recycling endosomes, resulting in recycling endosome aggregates. PMID: 27875067
    7. The results from this analysis indicated that Rab11a, Rab11c(Rab25) and Rab14 were expressed in a wide range of cell lines, including the human placental trophoblastic BeWo cell line. PMID: 28922401
    8. The results suggest that progressive assembly of vRNA segments may be cell type dependent and that vRNA may be transported through the cytoplasm without Rab11A-containing recycling endosomes in the absence of intact microtubules. PMID: 28724771
    9. Rac1-Rab11-FIP3 regulatory hub coordinates vesicle traffic with actin remodeling and T-cell activation PMID: 27154205
    10. In colorectal carcinoma, overexpression of Rab11 was found to promote collective cell migration through the increased distribution of E-cadherin, which enhanced cell-cell connections. PMID: 27696383
    11. A novel tribasic Golgi export signal directs cargo protein interaction with activated Rab11 and AP-1-dependent Golgi-plasma membrane trafficking. PMID: 26941330
    12. Analysis reveals novel aspects of the PI4KIIIb-Rab11 complex, specifically conformational changes induced in the switch regions of activated Rab11. PMID: 26756197
    13. Substituted V20 of Rab11A was found to interfere with proper localization of the water molecule, which mediated GTP hydrolysis, resulting in GTP being locked in an active form of Rab11A S20V. PMID: 26879265
    14. Data (including data from studies in knockout mice) suggest Munc13-4 binds to Rab11 and regulates trafficking of Rab11-containing vesicles; Munc13-4 appears to regulate final steps of Rab11-positive vesicle docking at plasma membrane in exocytosis. PMID: 26637356
    15. Rab11A and Rab11B differentially regulate intracellular trafficking of PAR1 through distinct endosomal sorting mechanisms PMID: 26635365
    16. Rab11a/Rab11 family-interacting protein 2-mediated VE-cadherin recycling is required for formation of adherens junctions and restoration of vascular endothelial barrier integrity. PMID: 26663395
    17. Mumps Virus release from the apical surface of polarized epithelial cells is facilitated by a Rab11-mediated transport system. PMID: 26378159
    18. Vaccinia virus traffics to early endosomes, where recycling endosome markers Rab11 and Rab22 are recruited to participate in subsequent virus trafficking prior to virus core uncoating in the cytoplasm. PMID: 26041286
    19. Rab11a-knockdown cells displayed mislocalization of STX3 and the presentation of microvilli in the basolateral compartment PMID: 25673875
    20. Rab11 co-localizes with alpha-synuclein in intracellular inclusions and modulates its aggregation, secretion and toxicity. PMID: 25092884
    21. PI4KIIIbeta likely plays a role in breast oncogenesis and that cooperation between Rab11a and PI4KIIIbeta represents a novel Akt activation pathway. PMID: 24962317
    22. Additionally, Rab11 recruitment is absent and Shigella-containing vacuolar rupture is delayed in the ipgD mutant that does not dephosphorylate PI(4,5)P2 into PI(5)P. PMID: 25299335
    23. Data demonstrated that Rab11 regulated E-cadherin expression and promoted colon cancer cell transformation. PMID: 25117932
    24. Rab11-dependent EGFR recycling has a role in synergistic cytotoxicity of afatinib and cetuximab against EGFR T790M PMID: 25446083
    25. Microvilli establishment required interaction between RAB8A and MYO5B, while loss of the interaction between RAB11A and MYO5B induced microvillus inclusions. PMID: 24892806
    26. Ubiquitylation and activation of RAB11a is promoted by a beta2 adrenergic receptor-HACE1 complex. PMID: 24190883
    27. Rab11A controls BACE1 recycling to endosomes and thus affects beta-amyloid production. PMID: 24373285
    28. these data provide a mechanism for how PI4KIIIbeta coordinates Rab11 and its effectors on PI4P-enriched membranes and also provide strategies for the design of specific inhibitors that could potentially target plasmodial PI4KIIIbeta to combat malaria. PMID: 24876499
    29. Mitochondrial remodeling was associated with increased proximity between Rab11a and mitochondrial membranes, changes in fusion-fission dynamics, and mitochondrial relocalization of the Drp1, which was regulated by the Rab11a effector protein FIP1/RCP. PMID: 24302731
    30. Data indicate that alpha2-macroglobulin (alpha2M*), through its receptor LRP1, induces cellular migration of Muller glial cells by a mechanism that involves MT1-MMP intracellular traffic to the plasma membrane by a Rab11-dependent recycling pathway. PMID: 23640058
    31. Dominant negative (GDP-locked)-Rab5 and -Rab11 reproduced the effects of inhibitors of tubulin and actin, respectively, on the cycling of bradykinin B receptor. PMID: 23454239
    32. These findings suggest that Rab11-FIP1 proteins participate in spatially and temporally distinct steps of the recycling process along a complex and dynamic tubular network in which Rab11-FIPs occupy discrete domains. PMID: 23283983
    33. The potential roles of the Rab11 pathway in the trafficking, assembly and budding steps of negative-strand viruses. PMID: 23176490
    34. Rab11 depletion gives rise to accumulation of recycling carriers containing endocytosed transferrin and transferrin receptor beneath the plasma membrane. PMID: 22685325
    35. findings show 2 mother centriole appendage proteins, centriolin and cenexin/ODF2, regulate association of the endosome components Rab11, the Evi5, and the exocyst at the mother centriole PMID: 22981775
    36. Rab11A/myosin Vb/Rab11-FIP2 complex frames two late recycling steps of langerin from the ERC to the plasma membrane PMID: 22420646
    37. TBC1D14 binds activated Rab11 but is not a GAP for Rab11, and loss of Rab11 prevents TBC1D14-induced tubulation of recycling endosomes. PMID: 22613832
    38. Our data suggest a selective role of PICK1 in clustering and reducing the recycling rates of PDZ domain binding partners sorted to the Rab11-dependent recycling pathway. PMID: 22303009
    39. Recycling was accompanied by dephosphorylation of VEGFR-2 between Rab4 and Rab11 vesicles and quantitatively and qualitatively altered signal output. PMID: 21586748
    40. Our results uncover a critical host factor with an essential contribution to influenza virus genome delivery and reveal a potential role for RAB11A in the transport of ribonucleoprotein cargo. PMID: 21525351
    41. The s propose that influenza virus ribonucleoproteins are routed from the nucleus to the pericentriolar recycling endosome (RE), where they access a Rab11-dependent vesicular transport pathway to the cell periphery. PMID: 21307188
    42. Rabin8 and Rab11 are associated with the Bardet-Biedl syndrome. PMID: 21273506
    43. Rab11a, but not Rab8a, is required for recycling of transferrin in nonpolarized cells. PMID: 21282656
    44. Rab11a played a prominent role in recycling of the wild type ss1-adrenergic receptors because dominant negative rab11a inhibited. PMID: 20727405
    45. Rab11a is an important regulator of TLR4 and Toll-receptor-associated molecule transport to E. coli phagosomes thereby controlling IRF3 activation from this compartment. PMID: 20933442
    46. Mutant prion protein expression is associated with an alteration of the Rab GDP dissociation inhibitor alpha (GDI)/Rab11 pathway. PMID: 19996123
    47. Results describe the involvement of two Rab GTPases, Rab9 and Rab11, in the trafficking of TRPC6. PMID: 20346379
    48. Transmission electron microscopy of thin sections confirmed that Rab11 depletion resulted in significant numbers of abnormally formed virus particles that had failed to pinch off from the plasma membrane. PMID: 20357086
    49. Rab11, in its GTP-bound form, interacts with Rabin8 and kinetically stimulates the guanine nucleotide-exchange activity of Rabin8 toward Rab8. PMID: 20308558
    50. mutation impairs a specific subset of Rab11 interactions, possibly those involved in cytoskeleton-based movements driving the slow recycling pathway PMID: 14699104

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor. Recycling endosome membrane; Lipid-anchor. Cleavage furrow. Cytoplasmic vesicle, phagosome. Cytoplasmic vesicle membrane.
  • 蛋白家族：
    Small GTPase superfamily, Rab family
  • 数据库链接：

    HGNC: 9760

    OMIM: 605570

    KEGG: hsa:8766

    STRING: 9606.ENSP00000261890

    UniGene: Hs.321541