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  4. Recombinant Human Ras association domain-containing protein 1 (RASSF1)

Recombinant Human Ras association domain-containing protein 1 (RASSF1)

  • 货号：
    CSB-YP885715HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP885715HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP885715HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP885715HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP885715HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    123F2; Cardiac specific ras association domain family 1 protein; cardiac-specific ras association domain family 1 protein; 123F2 ; NORE2A; OTTHUMP00000195503; OTTHUMP00000195504; OTTHUMP00000195505; OTTHUMP00000195507; Pancreas specific ras association domain family 1 protein; Ras association (RalGDS/AF 6) domain family member 1; Ras association domain-containing protein 1; RASF1_HUMAN; Rassf1; RASSF1A; RDA32; REH3P21; Tumor suppressor protein RDA32; WUGSC:H_LUCA12.5
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-344
  • 氨基酸序列
    SGEPELIEL RELAPAGRAG KGRTRLERAN ALRIARGTAC NPTRQLVPGR GHRFQPAGPA THTWCDLCGD FIWGVVRKGL QCARLSADCK FTCHYRCRAL VCLDCCGPRD LGWEPAVERD TNVDEPVEWE TPDLSQAEIE QKIKEYNAQI NSNLFMSLNK DGSYTGFIKV QLKLVRPVSV PSSKKPPSLQ DARRGPGRGT SVRRRTSFYL PKDAVKHLHV LSRTRAREVI EALLRKFLVV DDPRKFALFE RAERHGQVYL RKLLDDEQPL RLRLLAGPSD KALSFVLKEN DSGEVNWDAF SMPELHNFLR ILQREEEEHL RQILQKYSYC RQKIQEALHA CPLG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage.
  • 基因功能参考文献：
    1. Tissues from patients with serous and non-serous EOC showed significantly higher promoter methylation of EGFL7 and RASSF1 compared to benign cases. PMID: 29778234
    2. Transcriptome assay unclosed that RASSF1A promoted IL-6 expression in A375 cells, which in turn activate JAK2/STAT3 signaling. PMID: 30074127
    3. Rassf1a rs2236947 is a promising biomarker for patients treated with cetuximab plus chemotherapy. PMID: 27698403
    4. Low RASSF1A expression is associated with gastric cancer. PMID: 30106448
    5. methylation of BRCA1, RASSF1A and GSTP1 was found in 58.23 %, 74.68 % and 59.49 % of tumor tissues and 51.90 %, 63.29 % and 35.44 % of corresponding adjacent tissues, respectively PMID: 30049201
    6. Our study established that RASSF1A hypermethylation might have a potential value in the clinical diagnosis of colorectal cancer PMID: 30082160
    7. Effect of decitabine pretreatment on sensitivity of bladder cancer cells towards chemotherapeutic drugs was also studied. Decitabine treatment leads to restoration of RASSF1A, activation of hippo pathway followed by decreased expression of its oncogenic downstream targets (CTGF & CYR61). PMID: 29368096
    8. the use of RASSF1A promoter methylation could distinguish ovarian cancer and low malignant potential tumors. -RASSF1A promoter methylation may not be correlated with the clinical features and the survival of ovarian cancer patients. PMID: 29130987
    9. the methylation positive rates of SHOX2 with RASSF1A in stage III were both higher than the other stages of lung cancer PMID: 28325362
    10. Tested if RASSF1A polymorphisms associated with ovarian cancer and with tumor grade and stage. Five tagging single nucleotide polymorphisms (SNPs) (rs4688728G>T, rs72932987C>T, rs1989839C>T, rs2073497A>C, and rs2236947A>C) were evaluated. The CT genotype of rs1989839 was associated with the patients with ovarian cancer (P=0.001), and was significantly correlated with tumor grade and stage (P=0.008). PMID: 29670073
    11. No significant differences were presented in DNA methylation levels of RASSF1A and ATM between the sporadic breast cancer cases and the healthy controls. PMID: 29562656
    12. High Promoter Methylation of RASSF1A gene is associated with Breast Cancer. PMID: 29480000
    13. Hypermethylation of RASSF1 gene is associated with neuroendocrine tumors. PMID: 29851970
    14. The present work proved that there was a higher frequency of RASSF1A methylation and a decrease in its serum level in patients with ovarian cancer compared to controls as well as in the high-grade tumor patients compared to low grade ones and also in advanced ovarian tumor stage compared to early stages. PMID: 29098560
    15. Promoter methylation of RASSF1A can influence sensitivity to oxaliplatin-based chemotherapy, which can be used to predict outcomes for patients with stage II and III Colorectal Cancer PMID: 29128865
    16. The meta-analysis indicates a role of the RASSF1A promoter hypermethylation from peripheral blood in testicular germ cell tumors. PMID: 28871003
    17. RASSF1A is a tumour suppressor gene epigenetically dysregulated in NET. Aberrant methylation of RASSF1A has been reported in various tumours, but this is the first report of RASSF1A hypermethylation in TETs. PMID: 28838380
    18. Study shows that recombinant human bone morphogenetic protein-2 activates hippo signaling through RASSF1 in esophageal cancer cells PMID: 27230238
    19. There were significant associations between RASSF1A promoter hypermethylation and Oral Cancer risk.RASSF1A promoter hypermethylation exerted higher frequency in the Tongue Tumor than other site tumor in mouth. PMID: 29538221
    20. RASSF1A is expressed in differentiating stem cells. PMID: 29382819
    21. Hypermethylation of RASSF1A gene is associated with Colon Cancer. PMID: 26671036
    22. Data show that silencing DNA methyltransferase 1 (DNMT1) increased expression of tumor suppressor genes, RASSF1A and DAPK, in esophageal squamous cell carcinoma (ESCC) cells and ESCC xenograft in nude mice. PMID: 27286455
    23. Describe a novel interaction between Rheb and the tumor suppressor RASSF1A. This interaction may allow coordinate upregulation of Hippo while TOR is suppressed to modulate the balance of apoptosis and autophagy in lung tumor cells. PMID: 27034171
    24. Gestational age-dependent increase of hypermethylated RASSF1A; the fetal specific epigenetic marker in maternal plasma was demonstrated, in an Indian study group of normal pregnant women. PMID: 27165812
    25. the RASSF1 gene polymorphism influences molecular carcinogenesis and clinic pathological features of hepatocellular carcinoma within the Egyptian population PMID: 26921475
    26. We report for the first time that RASSF1A promoter methylation provides significant prognostic information in high-grade serous ovarian cancer patients. PMID: 28206954
    27. The main finding of this study was the significant association between RASSF1A promoter methylation and increased EC risk. PMID: 28669560
    28. Data suggest that the overexpression of DNMT3B4 may play an important role in human kidney tumorigenesis through chromosomal instability and methylation of RASSF1A. PMID: 28160561
    29. Methylation status of the RASSF1A gene promoter was strongly related to melanoma susceptibility but no significant difference was found between RASSF1A gene promoter methylation and the prognosis and clinical-pathological features of melanoma. PMID: 28207831
    30. Our results suggest a multifactorial role of RASSF1A in suppression of liver carcinogenesis PMID: 26980762
    31. rs1989839C/T is an important predictor of osteosarcoma risk and outcome. The CT genotype of rs1989839 is highly related to elevated risk of osteosarcoma. Furthermore, rs1989839C/T is also associated with the Enneking stage of osteosarcoma and risk of lung metastasis. One of the other 4 SNPs, rs2236947A/C, shows a borderline significance in predicting osteosarcoma risk. PMID: 27880743
    32. For the first time, we performed analysis of DNA methylation in SMARCB1/INI1-deficient sinonasal carcinomas, reporting on significantly higher methylation of RASSF1 gene in this neoplasm. PMID: 28069272
    33. High methylation in the RASSF1A promoter region is associated with lung cancer. PMID: 27716889
    34. GSTP1, APC and RASSF1 gene methylation in prostate cancer samples: comparative analysis of MS-HRM method and Infinium HumanMethylation450 BeadChip beadchiparray diagnostic value PMID: 28026816
    35. Compared with adjacent normal tissues, the methylation frequencies of WIF-1, RASSF1A, and CDH13 genes were significantly higher but the mRNA levels of these 3 genes were significantly lower in EC tissues. The survival rates of patients with WIF-1, RASSF1A, and CDH13 methylations were significantly lower than those of patients without methylation PMID: 27506957
    36. The presence of higher RASSF1A methylation levels in fine-needle aspiration cytology specimens of nodular goitre may be useful in the assessment of oncological risk. PMID: 26457492
    37. this meta-analysis demonstrated that RASSF1A promoter methylation is significantly associated with increased risk of renal cell cancer PMID: 27173228
    38. Overexpression of RASSF1A downregulated the cyclin D1. PMID: 26750098
    39. Results show that RASSF1A is involved in colorectal tumorigenesis. Its promotor region is bind to miR-181b stimulating its expression. PMID: 26935905
    40. The meta-analysis suggested that there was a significant association between aberrant RASSF1A methylation and head and neck squamous cell carcinoma. PMID: 26857374
    41. The present meta.analysis provides convincing evidence that the promoter methylation ratio of RASSF1A and p16 is associated with clinicopathological features in lung cancers, and could be used as effective biomarkers in early diagnosis in lung cancers. PMID: 27072261
    42. Loss of RASSF1A is associated with gastric cancer. PMID: 26735582
    43. Different genetic mechanisms seem to contribute to RASSF1 deregulation in this setting, but RASSF1 methylation does not seem to substantially affect RASSF1 isoforms expression. PMID: 26754001
    44. RASSF1C gene is not involved in Clear-cell renal cell carcinoma (ccRCC)progression and we propose that the measurements of RASSF1A mRNA levels in paired tumor-normal kidney tissue could serve as a new prognostic factor in ccRCC PMID: 26648328
    45. Results indicate that hypermethylation of tumor suppressor protein RASSF1A and docking protein 1 (DOK1) contributes to hepatocarcinogenesis and is associated to clinicopathological characteristics. PMID: 27078152
    46. Data indicate tumor suppressors TP73, RASSF1A, MLH1 and BRCA1 as possible biomarkers to distinguish Pleomorphic invasive lobular cancer (pleomorphic ILC} from classic ILC and infiltrative ductal cancer (IDC). PMID: 26392358
    47. Interaction between HPV infection and RASSF1A promoter methylation is associated with the development of cervical cancers. PMID: 26320446
    48. Hypermethylation of RASSF1A gene promoter is associated with esophageal squamous cell carcinoma. PMID: 25886188
    49. RASSF1A promotes the translocation of cytosolic PRMT5 to microtubules.PRMT5 co-localizes with RASSF1A on stabilized microtubules. PMID: 26482848
    50. Tumor-adjacent tissues showed higher methylation status of RASSF1A, HIN-1 and MGMT promoters. PMID: 26370119

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  • 亚细胞定位：
    [Isoform A]: Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Nucleus. Note=Localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. Colocalizes with MDM2 in the nucleus.; [Isoform C]: Nucleus. Note=Predominantly nuclear.
  • 组织特异性：
    Isoform A and isoform C are ubiquitously expressed in all tissues tested, however isoform A is absent in many corresponding cancer cell lines. Isoform B is mainly expressed in hematopoietic cells.
  • 数据库链接：

    HGNC: 9882

    OMIM: 605082

    KEGG: hsa:11186

    STRING: 9606.ENSP00000349547

    UniGene: Hs.476270