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  4. Recombinant Human RNA-binding protein Musashi homolog 1 (MSI1)

Recombinant Human RNA-binding protein Musashi homolog 1 (MSI1)

  • 货号：
    CSB-YP015033HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP015033HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP015033HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP015033HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP015033HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MSI1
  • Uniprot No.：
  • 别名：
    Msi 1; Msi1; MSI1H_HUMAN; Musashi homolog 1; Musashi RNA binding protein 1; Musashi-1; Musashi1; RNA binding protein Musashi homolog 1; RNA-binding protein Musashi homolog 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-362
  • 氨基酸序列
    METDAPQPGL ASPDSPHDPC KMFIGGLSWQ TTQEGLREYF GQFGEVKECL VMRDPLTKRS RGFGFVTFMD QAGVDKVLAQ SRHELDSKTI DPKVAFPRRA QPKMVTRTKK IFVGGLSVNT TVEDVKQYFE QFGKVDDAML MFDKTTNRHR GFGFVTFESE DIVEKVCEIH FHEINNKMVE CKKAQPKEVM SPTGSARGRS RVMPYGMDAF MLGIGMLGYP GFQATTYASR SYTGLAPGYT YQFPEFRVER TPLPSAPVLP ELTAIPLTAY GPMAAAAAAA AVVRGTGSHP WTMAPPPGST PSRTGGFLGT TSPGPMAELY GAANQDSGVS SYISAASPAP STGFGHSLGG PLIATAFTNG YH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
  • 基因功能参考文献：
    1. This provides proof of concept for the development of Gn-lip as a molecular therapy for colon cancer with MSI1/MSI2 overexpression. PMID: 30097032
    2. findings indicate that MSI1 plays a leading role in stress granule formation that grants cancer stem cell properties and chemoresistant stress granules in GBM, in response to stressful conditions via the PKR/eIF2alpha signalling cascade. PMID: 29486283
    3. Enhanced expression of Musashi-1 was observed in adenomyosis foci in comparison with endometrial cells. The most intensive staining was found in nodular adenomyosis, especially in epithelial cells during the secretion phase. These data attest to the role of somatic stem cells in the development and progression of various forms of adenomyosis. PMID: 28853072
    4. Msi1 acts as a stemness-associated gene in esophageal cancer cell lines and could serve as a prognostic marker in patients with ESCC. PMID: 28713964
    5. MSI1 and MSI2 bind and regulate the mRNA stability and translation of proteins operating in essential oncogenic signaling pathways..This review provides a current overview of Musashi as a cancer driver and novel therapeutic target. PMID: 28143872
    6. Musashi-1 has a role in regulating AKT-derived IL-6 autocrinal/paracrinal malignancy and chemoresistance in glioblastoma PMID: 27285760
    7. MSI1 was a target of miR-181a-5p, a microRNA involved in the regulation of cancer development. The expression levels of MSI1 and miR-181a-5p were negatively correlated in NSCLC. PMID: 29141252
    8. Musashi-1 interacts with the Zika genome and enables viral replication. PMID: 28572454
    9. Msi1promoted epithelial-mesenchymal transformation of cervical neoplasms via activation of the Wnt signaling pathway and contributing to poor prognosis. PMID: 28088346
    10. our results suggest a role for MSI1 in double-strand break repair and that its inhibition may enhance the effect of radiotherapy PMID: 27470713
    11. Musashi-1 expression was higher in Barrett esophagus and early esophageal adenocarcinoma compared to advanced adenocarcinoma. PMID: 28216140
    12. miR-761 and MSI1 are inversely expressed in ovarian cancer tissues. In conclusion, we demonstrated that miR-761 repressed ovarian cancer proliferation and invasion by targeting MSI1 PMID: 26563371
    13. Concomitant loss of function of both MSI1 is sufficient to abrogate the growth of human colorectal cancer cells, and Msi gene deletion inhibits tumorigenesis in several mouse models of intestinal cancer. PMID: 26673327
    14. Msi1 overexpression is associated with Esophageal Squamous Cell Carcinoma. PMID: 26294058
    15. studies highlight Msi reporters as a unique tool to identify therapy resistance, and define Msi signalling as a central regulator of pancreatic cancer PMID: 27281208
    16. Musashi-1 and ALDH1 expression are closely related to metastasis of ovarian adenocarcinoma. PMID: 25971681
    17. Suggest high level of Musashi-1 protein expression is associated with poor survival in endometrioid adenocarcinoma patients. PMID: 26191146
    18. Our results demonstrate that miR-137 acts as a tumor-suppressive miRNA in colorectal cancers and negatively regulates oncogenic Musashi-1 PMID: 25940441
    19. This review highlights both the essential contribution of RBPs to posttranscriptional regulation and the importance of the Musashi family as master regulators of male gamete development--{REVIEW} PMID: 25851660
    20. data suggest that in response to environmental cues that oppose cell cycle progression, regulation of Musashi function is required to promote target mRNA translation and cell fate transition PMID: 25888190
    21. oncogenic role in hepatocellular carcinoma may be through modulation of cell growth and cell cycle by activating Wnt pathway via direct downregulation of APC and DKK1 PMID: 24444033
    22. we suggest that Musashi-1, Lgr5, and pEGFR are overexpressed in human SIAs and may play roles in human SIA carcinogenesis and progression. PMID: 25773390
    23. The findings uncover a role for MSI1 as a central regulator of cell adhesion pathways that contribute to Glioblastoma by influencing cell adhesion, morphology, migration, and invasion. PMID: 26100017
    24. Msi1 was significantely overexpressed in 41.5 % of tumor samples. PMID: 24163304
    25. High Msi1 promotes tumor growth and cell proliferation by targeting cell cycle checkpoint proteins p21, p27 and p53 in cervical carcinomas. PMID: 25362645
    26. Knockdown of endogenous Msi1 protein inhibited the growth and tumor formation by activating the cell cycle suppressor p21cip PMID: 25394506
    27. Msi proteins contribute to an epithelial gene expression program in neural and mammary cell types. PMID: 25380226
    28. Msi1 is a mediator of TRAIL resistance in HCC cells PMID: 25747387
    29. an association between a Musashi-1 genotype and lung cancer risk PMID: 24787949
    30. The principal findings included significantly higher level of Msi-1 expression in younger gastric cancer patients compared to older ones, and probable association of tumor Msi-1 expression in young gastric cancer patients with more aggressive tumor type. PMID: 24452324
    31. Musashi-1 mediates the downregulation of the 26S proteasome by binding to the mRNA of NF-YA, the transcriptional factor regulating 26S proteasome subunit expression PMID: 24022895
    32. Aberrant Musashi-1 expression in adenomyosis may be involved in the pathogenesis and pathophysiology of adenomyosis, but it does not correlate with changes in serum estradiol or progesterone levels. PMID: 24365097
    33. High levels of Musashi-1 were associated with gliomas. PMID: 24057325
    34. NOTCH3 signaling regulates MUSASHI-1 expression in metastatic colorectal cancer cells. PMID: 24525742
    35. Msi-1-positive cells may play a key role in the early events of gastric carcinogenesis and may be involved in invasion and metastasis of gastric cancer cells. PMID: 24151393
    36. our data demonstrated that Musashi 1 is highly and un-differentially expressed in both examined tumoral and apparently normal bladder tissues. PMID: 24061461
    37. Msi1 regulates the proliferation of stem-like spheroid lung tumor cells in associated with Notch and Wnt signaling. PMID: 23715514
    38. study provides novel insights into Hedgehog/Msi1 signaling and its role in the regulation of the self-renewal capability of human umbilical cord blood (UCB)-derived mesenchymal stem cells PMID: 23418578
    39. was a statistical difference between the MSI1 gene expression in intestinal versus diffuse type gastric cancer PMID: 23164718
    40. Msi1 has different expression patterns in glioma from Grade I to Grade IV, and the different expression patterns play diverse roles in angiogenesis of glioma. PMID: 23017339
    41. Msi1 functions as a regulator of multiple processes in medulloblastoma formation and could become an important therapeutic target. PMID: 22985791
    42. study concludes that tumor suppressor miRNAs are direct and influential regulators of Msi1, affecting its expression pattern during tumorigenesis of malignant nervous system tumors PMID: 21881409
    43. We conclude that Ishikawa cells contain a subpopulation of cells with stem cell-like properties. Musashi-1 modulates endometrial carcinoma cell cycle progression and apoptosis via the stemness-related factors Notch-1, Hes-1 and p21(WAF1/CIP1) PMID: 21165952
    44. Musashi1 is regulated by HuR via mRNA translation and stability in glioblastoma cells. PMID: 22258704
    45. Overexpression of MSI1 was sufficient to increase Hes1, stimulate proliferation, inhibit apoptosis and reduce insulin expression, whereas Msi1 knockdown had the converse effects on proliferation and insulin expression. PMID: 22113197
    46. Our study suggested that Msi-1 and/or ALDH1 expression might be closely related to the carcinogenesis, progression, clinical biological behaviors, and prognosis of gallbladder adenocarcinoma. PMID: 22012766
    47. Msi-1 positive cells have several characteristics of stem cells, such as high proliferative potentiality and multipotency PMID: 21751144
    48. CD44 and Musashi-1 are frequently expressed in both premalignant gastric lesions and invasive gastric cancer, whereas CD133 expression is restricted mainly to neoplastic tissues. PMID: 21829201
    49. Overexpression of Msi-1 was associated with lymph node metastasis in colon cancer. PMID: 21442350
    50. APC as a key factor in MSI1 regulation through Wnt signaling and identify APC mRNA as a novel target of translational inhibition by MSI1. PMID: 21199875

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Musashi family
  • 组织特异性：
    Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.
  • 数据库链接：

    HGNC: 7330

    OMIM: 603328

    KEGG: hsa:4440

    STRING: 9606.ENSP00000257552

    UniGene: Hs.158311