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  4. Recombinant Human RAS guanyl-releasing protein 1 (RASGRP1), partial

Recombinant Human RAS guanyl-releasing protein 1 (RASGRP1), partial

  • 货号：
    CSB-YP019362HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP019362HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP019362HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP019362HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP019362HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    RASGRP1; RASGRPRAS guanyl-releasing protein 1; Calcium and DAG-regulated guanine nucleotide exchange factor II; CalDAG-GEFII; Ras guanyl-releasing protein
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions as a calcium- and diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. Activates the Erk/MAP kinase cascade. Regulates T-cell/B-cell development, homeostasis and differentiation by coupling T-lymphocyte/B-lymphocyte antigen receptors to Ras. Regulates NK cell cytotoxicity and ITAM-dependent cytokine production by activation of Ras-mediated ERK and JNK pathways. Functions in mast cell degranulation and cytokine secretion, regulating FcERI-evoked allergic responses. May also function in differentiation of other cell types.
  • 基因功能参考文献：
    1. Histidine 212 is located at the fulcrum of these conformational changes, and structural features in its vicinity are consistent with its function as a pH-dependent switch. PMID: 28952923
    2. The rs7170151 in RASGRP1 showed novel associations in IgA nephropathy. PMID: 27804980
    3. The marked differences between RasGRP3 and RasGRP1 in membrane interaction necessarily will contribute to their different behavior in cells. PMID: 28912101
    4. Cytokines IL-2/7/9 stimulation activates PI3K/Akt pathways downstream of Ras in RasGRP1 T-cell acute lymphoblastic leukemia (T-ALL). PMID: 26549032
    5. results demonstrate the critical role of CalDAG-GEFI in rapid alphaIIbbeta3 activation of human platelets. PMID: 27663674
    6. Low-level expression of CD-GEFI impairs platelet activation, leading to protection from thrombosis, but not to marked bleeding in mice. PMID: 27417588
    7. This study shows that deficiency in RASGRP1 results in a previously unknown primary immunodeficiency disease, and that RASGRP1 plays role in immune cell signaling and function in T cells, B cells and NK cells. It also identifies a previously unknown role for RASGRP1 in the dynamic regulation of the cytoskeleton, and identify lenalidomide as a potpotential treatment option for this immunodeficiency. PMID: 27776107
    8. This study aimed to replicate and verify the association of RASGRP1 tag single-nucleotide polymorphisms with T2D in a Chinese Han population. PMID: 26076219
    9. present a crystal structure of a fragment of RasGRP1 in which the Ras-binding site is blocked by an interdomain linker and the membrane-interaction surface of RasGRP1 is hidden within a dimerization interface PMID: 23908768
    10. A genome-wide association study identifies GRK5 and RASGRP1 as type 2 diabetes loci in Chinese Hans. PMID: 22961080
    11. This is the first study aimed at evaluating CalDAG-GEFI gene sequences in people with mucocutaneous bleeding of unknown cause. PMID: 21815871
    12. PAQR10 and PAQR11 are able to interact with RasGRP1, a guanine nucleotide exchange protein of Ras, and increase Golgi localization of RasGRP1. The C1 domain of RasGRP1 is both necessary and sufficient for the interaction of RasGRP1 with PAQR10/PAQR11. PMID: 21968647
    13. cooperation between aberrant expression of RasGRP1, a strong activator of Ras, and secondary gain-of-function mutations of NOTCH1 have an important role in T-cell leukemogenesis PMID: 22116551
    14. remission in systemic lupus erythematosus activity associated with decreased RasGRP-1 expression in lymphocytes PMID: 21976405
    15. Basal LAT-diacylglycerol-RasGRP1 signals in T cells maintain TCRalpha gene expression. PMID: 21966541
    16. SDF-1 treatment of T cells induced the formation of a novel molecular signaling complex containing RasGRP1, Galphai2, and ZAP-70. PMID: 21856938
    17. Data show that the RASGRP1/APTX gene expression ratio was higher in the responder while the AKAP13 expression was higher in the non-responders. PMID: 19960345
    18. in response to Src-dependent activation of phospholipase Cgamma1, the Ras guanine nucleotide exchange factor RasGRP1 translocated to the Golgi where it activated Ras PMID: 12845332
    19. RasGRP binds to three DAG molecular species, but only DAG-AA and DAG-DHA participate in the modulation of RasGRP-mediated activation of MAP kinases in Jurkat T cells PMID: 14583629
    20. RasGRP1 transgene conferred pre-TCR-independent survival and proliferation of immature thymocytes, suggesting that deregulated expression of RasGRP1 promotes lymphomagenesis by expanding the pool of thymocytes which are susceptible to transformation. PMID: 15829980
    21. RasGRP1-/- mast cells had markedly reduced degranulation and cytokine production. PMID: 17190838
    22. The unusual RasGRP-SOS interplay results in sensitive and robust Ras activation that cannot be achieved with either activator alone. PMID: 17283063
    23. CalDAG-GEFI as a critical regulator of inside-out integrin activation in human T lymphocytes, neutrophils, and platelets. PMID: 17576779
    24. These results suggest that SKAP55 modulates signal transduction from the T cell antigen receptor to Ras by binding to RasGRP1. PMID: 17658605
    25. Essential role for Rap1 GTPase and its guanine exchange factor CalDAG-GEFI in LFA-1 mediated human T-cell adhesion. PMID: 17702895
    26. identify 13 new splice variants of the human RasGRP1 gene PMID: 17878389
    27. Stimulation of human mast cells by activated T cells leads to N-Ras activation through Ras guanine nucleotide releasing protein 1. PMID: 18760455
    28. Studied RASGRP1, found 1 locus, mapping to a linkage disequilibrium (LD) block at chr15q14, reached statistical significance by combining results from two markers. PMID: 19465406

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  • 相关疾病：
    Systemic lupus erythematosus (SLE)
  • 亚细胞定位：
    Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.
  • 蛋白家族：
    RASGRP family
  • 组织特异性：
    Expressed in brain with higher expression in cerebellum, cerebral cortex and amygdala. Expressed in the hematopoietic system. Expressed in T-cells (at protein level). Expressed in NK cells (at protein level).
  • 数据库链接：

    HGNC: 9878

    OMIM: 152700

    KEGG: hsa:10125

    STRING: 9606.ENSP00000310244

    UniGene: Hs.591127