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  4. Recombinant Human Prothrombin (F2)

Recombinant Human Prothrombin (F2)

In Stock
  • 货号：
    CSB-YP007923HU
  • 规格：
    ￥2208
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    coagulation factor II (thrombin); Coagulation factor II; F2; Factor II; Prepro coagulation factor II; Prothrombin; prothrombin B-chain; PT; RPRGL2; serine protease; THPH1; THRB; THRB_HUMAN; Thrombin heavy chain
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Yeast
  • 分子量：
    67.3kDa
  • 表达区域：
    44-622aa
  • 氨基酸序列
    ANTFLEEVRKGNLERECVEETCSYEEAFEALESSTATDVFWAKYTACETARTPRDKLAACLEGNCAEGLGTNYRGHVNITRSGIECQLWRSRYPHKPEINSTTHPGADLQENFCRNPDSSTTGPWCYTTDPTVRRQECSIPVCGQDQVTVAMTPRSEGSSVNLSPPLEQCVPDRGQQYQGRLAVTTHGLPCLAWASAQAKALSKHQDFNSAVQLVENFCRNPDGDEEGVWCYVAGKPGDFGYCDLNYCEEAVEEETGDGLDEDSDRAIEGRTATSEYQTFFNPRTFGSGEADCGLRPLFEKKSLEDKTERELLESYIDGRIVEGSDAEIGMSPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPWDKNFTENDLLVRIGKHSRTRYERNIEKISMLEKIYIHPRYNWRENLDRDIALMKLKKPVAFSDYIHPVCLPDRETAASLLQAGYKGRVTGWGNLKETWTANVGKGQPSVLQVVNLPIVERPVCKDSTRIRITDNMFCAGYKPDEGKRGDACEGDSGGPFVMKSPFNNRWYQMGIVSWGEGCDRDGKYGFYTHVFRLKKWIQKVIDQFGE
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The process of expressing the recombinant human F2 protein in the yeast requires the recombinant DNA gene formed by the integration of encoding gene for the 44-622aa of the human F2 protein and N-terminal 6xHis tag sequence, the expression vector that the recombinant DNA gene inserts into, the yeast that provided the necessary macromolecules and components for transcription and translation of the cloned expression vector. After isolation and purification, this N-terminal 6xHis-tagged recombinant F2 protein was obtained. This recombinant F2 protein is characterized by high purity (>90%, SDS-PAGE). This F2 protein ran along the gel to the band of approximately 66-105 kDa molecular weight.

    F2 is a gene providing instruction of making a protein named prothrombin (also known as coagulation factor II) in human and belongs to peptidase S1 family. This protein can be cleaved into 4 chains, including activation peptide fragment 1, activation peptide fragment 2, thrombin light chain and thrombin heavy chain. It is the precursor of thrombin, which acts as a serine protease cleaving bonds after Arg and Lys and converting soluble fibrinogen into insoluble strands of fibrin and activating factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
  • 基因功能参考文献：
    1. When endothelial cells are incubated with antiphospholipid antibodies thrombin generation is increased. PMID: 30031291
    2. No significant difference in FVL genotype between patients and controls was observed, whereas high frequencies of PRT G20210A, MTHFR C677T and MTHFR A1298C mutations in the Hb S patients PMID: 30200836
    3. This study proved that Berberine (BBR) is a direct thrombin inhibitor that has activity in inhibiting thrombin-induced platelet aggregation. BBR may be a potential candidate for the development of safe and effective thrombin-inhibiting drugs PMID: 28276481
    4. Thrombin generation had a limited impact as biomarker of venous thromboembolism. PMID: 30093507
    5. results explain the reduced pro-coagulant activity of the W215A mutant and demonstrate the allosteric connection between Trp215, the sodium-binding loop, and the active site PMID: 29634247
    6. Polymorphisms of F2, PROC, PROZ, and F13A1 Genes are Associated With Recurrent Spontaneous Abortion in Chinese Han Women. PMID: 29363996
    7. the prothrombotic activity of FII is the result of a polymorphism and of a missense mutation, whereas that of FV derives only from a polymorphism. The observation that a clotting factor defect may be associated with both bleeding or venous thrombosis depending on the site of the mutation has caused an extensive reevaluation of the blood clotting mechanism. PMID: 29690772
    8. Findings provide evidence for a relationship between two genes-three mutations-of the cardiovascular disease (CVD genes panel and recurrent pregnancy loss (RPL). These genotypes include the heterozygous state of the factor II G20210A mutation. PMID: 29974397
    9. digestion of thrombin by P. aeruginosa elastase leads to the release of the C-terminal thrombin-derived peptide FYT21, which inhibits pro-inflammatory responses to several pathogen-associated molecular patterns. PMID: 27181065
    10. Prothrombin gene mutation is rare in Budd-Chiari syndrome patients in India. PMID: 29616413
    11. In HBV-related cirrhosis, the combination of miR-122, AFP and PIVKA-II enables the identification of patients at higher risk of hepatocellular carcinoma development. PMID: 28650134
    12. Data, including data using network analysis, suggest that angiotensinogen (AGT), mitogen-activated protein kinase-14 (MAPK14), and prothrombin (F2) in placental villous tissues are core factors in early embryonic development; these studies involved proteomics and bioinformatics analysis of altered protein expression in placental villous tissue from early recurrent miscarriage patients in comparison to control tissues. PMID: 29277264
    13. Arg596Gln mutation is a risk factor for Chinese patients with venous thromboembolism due to its moderately decreased clotting activity but strong resistance to antithrombin inhibition. PMID: 29331940
    14. The induction of TG by BXPC3 cells was mainly driven by the TF pathway while TG generation triggered by MCF7 cells was also driven by FXII activation. PMID: 29075790
    15. we were not able to confirm the association between the polymorphisms of f5, f2, and mthfr and pregnancy loss in Bosnian women PMID: 28488549
    16. Platelets were activated in antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) patients, and such activation was at least partially attributed to the thrombin-protease-activated receptors (PARs) pathway. PMID: 29141676
    17. The meta-analysis enabled us to underline the importance of DCP in the refinement of the eligibility criteria of hepatocellular cancer (HCC)patients for liver transplant (LT). This information, based on Japanese studies performed in the setting of living-donor LT only, needs further validation in the Western world both in the setting of post-mortem and living-donor LT. PMID: 28561879
    18. HCC [hepatocellular carcinoma] patients who are sero-positive for DCP [Des-gamma-carboxyprothrombin] and sero-negative for AFP[Alpha-fetoprotein] have significantly higher levels of serum ALT[alanine aminotransferase]; serum ALT levels may be of diagnostic importance in AFP-negative, HBV-related HCC [hepatitis B virus-related hepatocellular carcinoma] patients. PMID: 29192630
    19. Evaluation of initial thrombin generation is useful to distinguish between beneficial coagulation activation and hazardous haemostatic alteration, and to predict multiorgan dysfunction development and poor outcome in septic patients. PMID: 28166112
    20. Prothrombin showed statistically significant differences between the groups of septic and non-septic burn patients. PMID: 28454850
    21. The data indicate that prothrombin Arg596 missense mutations lead to antithrombin (AT) and thrombomodulin (TM) resistance in the variant thrombins and suggest that prothrombin Arg596 is important for AT- and TM-mediated anticoagulation. PMID: 27604259
    22. application of electrical stimulus modifies the molecular interactions within the complex and consequently, electrical field can be used to modulate the association between the thrombin and its aptamer. PMID: 27874042
    23. The G20210A prothrombin mutation carriers after venous thromboembolism have unfavourable fibrin clot characteristics, including lower permeability and impaired lysis compared with non-carriers. Rivaroxaban treatment cannot abolish more prothrombotic fibrin clot phenotype observed in prothrombin mutation carriers following VTE. PMID: 28771277
    24. prothrombin-20210-mutations are playing a significant role in the pathogenesis of cerebral sinus vein thrombosis, but not in arterial ischemic stroke PMID: 28869458
    25. Patients with early onset preeclampsia are characterised by an attenuated coagulation response characterised by reduced thrombin generation stimulated by low-dose TF and elevated plasma TFPI activity. PMID: 28569919
    26. this study demonstrated that thrombin and factor Xa cleavage sites on HEV pORF1 are obligatory for HEV replication. PMID: 29321328
    27. increased frequency of factor V Leiden G1691A and prothrombin G20210A mutation in venous thromboembolism patients indicates a significant role of these mutations in the development of VTE in the Kashmiri population PMID: 29454086
    28. The results suggest that an increased plasma thrombin potential is characteristic in patients with clinically stable coronary artery disease, irrespective of previous myocardial infarction history and independent of traditional cardiovascular risk factors. PMID: 28477533
    29. Enhanced thrombin generation is driven in asthma by a systemic inflammatory state mediated by IL-6 and to a lesser extent TNFalpha, however, not periostin. TNFalpha might contribute to impaired fibrinolysis. PMID: 28429138
    30. Endogenous thrombin potential measured in presence of thrombomodulin is enhanced in patients with intracranial atherosclerotic disease (ICAD), supporting that thrombomodulin-protein C pathways is relevant in transient ischaemic attacks from ICAD. PMID: 28505525
    31. PIVKA II, when combined with AFP, may be considered as a screening test for hepatocellular carcinoma due to its high negative predictive value. PMID: 28652441
    32. Thrombin activated platelet releasing exosomes convey miRNA between cells. miRNA-223 regulates the expression of molecules adhesion including ICAM-1. miRNA-223 downregulated ICAM-1 mainly by impacting NF-kappaB and the MAPK pathway. PMID: 28460288
    33. Thrombin binding to extra-cellular loop II (ECLII) of PAR4 is important for its cleavage and activation of PAR4. PMID: 28448853
    34. These findings suggest that contraction-dependent TGF-beta activation could be a mechanism by which thrombin leads to the development of asthmatic airway remodeling. PMID: 29428600
    35. Case Report: Paradoxical bleeding and thrombotic episodes of dysprothrombinaemia due to a homozygous Arg382His mutation I prothrombin. PMID: 27975099
    36. PARP-1 activates prothrombin gene transcription and that the excessive prothrombin gene transcription induces des-gamma-carboxy prothrombin (DCP) production in DCP-producing hepatocellular carcinoma cells. PMID: 28384634
    37. the routine screening of patients with NAIS for F5 G1691A, F2 G20210A and MTHFR C677T gene mutations might not be justified, and additional prothrombotic mechanisms should be considered. PMID: 27619728
    38. Although rare, the prothrombin Belgrade mutation represents strong thrombophilia with early onset of thrombosis in a large Serbian pedigree PMID: 28075532
    39. There were no significant differences in factor V and factor II genotypes between infertile men and normal controls. PMID: 27815482
    40. histone H4 has a rapid and drastic inhibitory effect on prothrombin activation by prothrombinase that is likely to dominate pathophysiology PMID: 27359051
    41. prothrombotic mutations in factor V Leiden and prothrombin as well as older age are risk factors for venous thrombosis PMID: 27377285
    42. p300 inhibition attenuates both thrombin induced-CCL2 expression and histone H3 and H4 acetylation in HLFs, suggesting that p300 is involved in thrombin-induced CCL2 expression via hyperacetylating histone H3 and H4. PMID: 28407300
    43. p300-dependent histone H3 acetylation and C/EBPbeta-regulated IKKbeta expression contribute to thrombin-induced IL-8/CXCL8 expression in human lung epithelial cells. PMID: 28428115
    44. Meta-analysis of 30 case-control studies showed that the FII 20210G>A polymorphism is associated with arterial ischemic stroke in both pediatric and young adult patients. PMID: 28160964
    45. Thrombin, via PAR1 activation, synergistically augments LPS-induced Human endometrial endothelial cells production of chemokines involved in immune cell recruitment and survival, suggesting a mechanism by which intrauterine abruption and bacterial infection may together be associated with an aggravated uterine inflammatory response. PMID: 27108773
    46. Our findings suggest that hereditary thrombophilia associated with retinal vein occlusion is more likely to be multigenic than caused by any single risk factor. PMID: 28085526
    47. Clinical significance of prothrombin G20210A mutation in homozygous patients. PMID: 28707429
    48. The prevalence of FVL polymorphism (16.3 %) was higher in retinopathy of prematurity (ROP) patients than control subjects in this Turkish cohort. We suggest a possible association of FVL mutation with ROP at the end of the study. PMID: 27018927
    49. Data suggest that, for all coagulation proteins tested (prothrombin, factor X, activated factor VII, activated protein C), tighter binding to lipid bilayers (lower Kd) is observed as the proportion of anionic phospholipid increases. These studies were conducted in high-throughput screening using phospholipid bilayers in nanodiscs with multiplexed silicon photonic sensor (micro-ring resonator) array technology. PMID: 28801460
    50. Factor Va reduced by 100-fold the apparent Kd of myosin for factor Xa (Kd approximately 0.48 nM), primarily by reducing koff, indicating formation of a stable ternary complex of myosin:Xa:Va. PMID: 27421960

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  • 相关疾病：
    Factor II deficiency (FA2D); Ischemic stroke (ISCHSTR); Thrombophilia due to thrombin defect (THPH1); Pregnancy loss, recurrent, 2 (RPRGL2)
  • 亚细胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Peptidase S1 family
  • 组织特异性：
    Expressed by the liver and secreted in plasma.
  • 数据库链接：

    HGNC: 3535

    OMIM: 176930

    KEGG: hsa:2147

    STRING: 9606.ENSP00000308541

    UniGene: Hs.655207