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  4. Recombinant Human Protein sprouty homolog 1 (SPRY1)

Recombinant Human Protein sprouty homolog 1 (SPRY1)

  • 货号：
    CSB-YP022621HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP022621HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP022621HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP022621HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP022621HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    hSPRY1; Protein sprouty homolog 1; Sprouty 1; Sprouty homolog 1 antagonist of FGF signaling; Sprouty homolog 1; Spry-1; Spry1; SPY1_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-319
  • 氨基酸序列
    MDPQNQHGSG SSLVVIQQPS LDSRQRLDYE REIQPTAILS LDQIKAIRGS NEYTEGPSVV KRPAPRTAPR QEKHERTHEI IPINVNNNYE HRHTSHLGHA VLPSNARGPI LSRSTSTGSA ASSGSNSSAS SEQGLLGRSP PTRPVPGHRS ERAIRTQPKQ LIVDDLKGSL KEDLTQHKFI CEQCGKCKCG ECTAPRTLPS CLACNRQCLC SAESMVEYGT CMCLVKGIFY HCSNDDEGDS YSDNPCSCSQ SHCCSRYLCM GAMSLFLPCL LCYPPAKGCL KLCRRCYDWI HRPGCRCKNS NTVYCKLESC PSRGQGKPS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Inhibits fibroblast growth factor (FGF)-induced retinal lens fiber differentiation, probably by inhibiting FGF-mediated phosphorylation of ERK1/2. Inhibits TGFB-induced epithelial-to-mesenchymal transition in lens epithelial cells.
  • 基因功能参考文献：
    1. Study demonstrated that Spry1 has a role in supporting mitogen-induced vascular hyperplasia, at least in part by promoting Akt/Rb signaling and increasing mitogen-induced cyclinD1 expression and decreasing p27Kip1 expression. PMID: 29105817
    2. SPRY1 maybe a negative feedback regulator in normal human epidermal keratinocytes. PMID: 30039569
    3. FREM2 is thus proposed as a novel GB biomarker and a putative biomarker of glioblastoma stem cells. Both FREM2 and SPRY1 are expressed on the surface of the GB cells, while SPRY1 alone was found overexpressed in the cytosol of non-malignant astrocytes. PMID: 29734672
    4. Inhibition of miR-29b could attenuate atherosclerosis by inhibiting the SPRY1/MAPK signaling pathway and inflammation in aorta. PMID: 29398368
    5. During adipogenesis and osteogenesis, TNF-alpha significantly increased Spry1 levels and overexpression of miR-21 dramatically decreased Spry1 levels in the presence of TNF-alpha, indicated important roles of miR-21 in modulating link between TNF-alpha and Spry1. Our findings introduce a molecular mechanism in which TNF-alpha suppresses adipogenic and osteogenic differentiation of PDLSCs by inhibiting miR-21/Spry1 func... PMID: 28946056
    6. Although the expression of SPRY1 was low when compared with other tumors, SPRY1 was variably expressed in primary Ewing sarcoma tumors and higher expression levels were significantly associated with improved outcome in a large patient cohort. PMID: 27375017
    7. SPRY1 and SPRY2 mRNA transcripts were significantly upregulated in human CRC. Suppression of SPRY2 repressed AKT2 and EMT-inducing transcription factors and significantly increased E-cadherin expression. Concurrent downregulation of SPRY1 and SPRY2 also increased E-cadherin and suppressed mesenchymal markers in colon cancer cells. PMID: 26434583
    8. Spry1 plays a selective role in at least a subset of triple-negative breast cancer to promote the malignant phenotype via enhancing EGF-mediated mesenchymal phenotype. PMID: 26976794
    9. Suppression of SPRY1 by age-associated methylation inhibits the replenishment of the muscle stem cell pool. PMID: 26526994
    10. Cosuppression of Sprouty and Sprouty-related negative regulators of FGF signalling in prostate cancer PMID: 26075267
    11. There is an inverse correlation between the expression of Spry1 and growth, proliferation, invasion and migration of ovarian cancer cells. PMID: 24932220
    12. A random population of LNCaP prostate cancer cells comprises a heterogeneous group of cells with different androgen-deprivation sensitivities and potential for invasiveness; expression levels of 2 genes known to be regulated by miR-21, an androgen-regulated microRNA, SPRY1 and JAG1 were lower in an androgen insensitive clone, than an androgen sensitive clone. PMID: 24604720
    13. The Spry1 acts as a sensor of mitogenic activity that not only attenuates RTK signaling but also induces a cellular senescence response to avoid uncontrolled proliferation. PMID: 24270409
    14. Sprouty1 expression is not connected with mitogenic signaling and cell proliferation. PMID: 23616430
    15. Spry1 and Spry4 have opposing roles in VSMC phenotypic modulation, and Spry1 maintains the VSMC differentiation phenotype in vitro in part through an Akt/FoxO/myocardin pathway. PMID: 23554919
    16. Re-expression of SPRY1, a repressed target of BCL11B, limits the transformation capacity of Ewing sarcoma cells. PMID: 23527175
    17. miR-21 promotes robust fibrogenic EMT of EMCs, in part by directly targeting PDCD4 and SPRY1. PMID: 23441172
    18. Feedback regulations of miR-21 and MAPKs via Pdcd4 and Spry1 are involved in arsenite-induced cell malignant transformation. PMID: 23469214
    19. Results suggest that the SPRY1 gene is an important genetic factor for determining the risk of both obesity and osteoporosis in humans. PMID: 23146288
    20. siRNA-mediated knockdown of Spry1, Spry2, or Spry4 promotes IFN-inducible antileukemic effects in vitro and results in enhanced suppressive effects on malignant hematopoietic progenitors from patients with polycythemia vera. PMID: 23074222
    21. The spatio-temporal expression of Decorin, Semaphorin6D, SPARC, Spry-1 and Tsukushi in developing prostate is described and observed similar mesenchymal expression patterns in rat and human. PMID: 22880013
    22. Spry1 is a target for miR-21-mediated gene silencing. PMID: 21826097
    23. Results suggest that SPRY1 is an endogenous angiogenesis inhibitor. PMID: 20813052
    24. hSpry1 and HAX1 proteins are putative candidate proteins that interact with uPAR. PMID: 20696135
    25. Data show that upregulation of Spry1 in HUVECs inhibits tube formation and is associated with decreased proliferation and increased expression of p21 and p27. PMID: 20054616
    26. Sprouty1 and Sprouty2 effect cortical cell proliferation, differentiation, and the expression of genes regulating progenitor identity in the ventricular zone. PMID: 20237272
    27. SPRY1 supported formation of activated ERK and mitogen-activated protein/ERK kinase and was essential for ERMS cell proliferation and survivalin rhabdomyosarcoma. PMID: 20068162
    28. The bimodal regulation of epidermal growth factor signaling by human Sprouty proteins PMID: 11983899
    29. Expression analysis of SPRY1a and SPRY1b shows that they are hardly expressed in adult human, but have different expression patterns in fetus, which confirmed that SPRY1 is an important gene during fetal development PMID: 14533010
    30. Expression of an SPRY1 alternative transcript is presumed to be specific for fetal tissues and and is moderately expressd in prostate csncer tissues and cell lines. PMID: 16954433
    31. DNA methylation in the Sprouty1 promoter region is responsible for downregulating Sprouty1 expression in prostate cancer PMID: 19164898
    32. Sprouty1 induced by TCR signal negatively regulates further TCR signaling by interacting with proximal signaling molecules in immune synapse, providing a novel regulatory mechanism of T cells. PMID: 19915061

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  • 亚细胞定位：
    Cytoplasm. Membrane; Peripheral membrane protein. Note=Found in the cytoplasm in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF (epidermal growth factor).
  • 蛋白家族：
    Sprouty family
  • 数据库链接：

    HGNC: 11269

    OMIM: 602465

    KEGG: hsa:10252

    STRING: 9606.ENSP00000343785

    UniGene: Hs.744854