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  4. Recombinant Human Protein phosphatase 1D (PPM1D)

Recombinant Human Protein phosphatase 1D (PPM1D)

  • 货号：
    CSB-YP018491HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018491HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP018491HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018491HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018491HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    EC 3.1.3.16; p53 induced protein phosphatase 1; p53-induced protein phosphatase 1; PP2C delta; PP2C-delta; PP2CD; Ppm1d; PPM1D_HUMAN; Protein phosphatase 1D; protein phosphatase 1D magnesium-dependent delta isoform ; Protein phosphatase 2C delta isoform; Protein phosphatase 2C isoform delta; Protein phosphatase magnesium dependent 1 delta; Protein phosphatase magnesium-dependent 1 delta; Protein phosphatase Mg2+/Mn2+ dependent 1D; Protein phosphatase Wip1; Wild type p53 induced phosphatase 1; WIP 1; WIP1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-605
  • 氨基酸序列
    MAGLYSLGVS VFSDQGGRKY MEDVTQIVVE PEPTAEEKPS PRRSLSQPLP PRPSPAALPG GEVSGKGPAV AAREARDPLP DAGASPAPSR CCRRRSSVAF FAVCDGHGGR EAAQFAREHL WGFIKKQKGF TSSEPAKVCA AIRKGFLACH LAMWKKLAEW PKTMTGLPST SGTTASVVII RGMKMYVAHV GDSGVVLGIQ DDPKDDFVRA VEVTQDHKPE LPKERERIEG LGGSVMNKSG VNRVVWKRPR LTHNGPVRRS TVIDQIPFLA VARALGDLWS YDFFSGEFVV SPEPDTSVHT LDPQKHKYII LGSDGLWNMI PPQDAISMCQ DQEEKKYLMG EHGQSCAKML VNRALGRWRQ RMLRADNTSA IVICISPEVD NQGNFTNEDE LYLNLTDSPS YNSQETCVMT PSPCSTPPVK SLEEDPWPRV NSKDHIPALV RSNAFSENFL EVSAEIAREN VQGVVIPSKD PEPLEENCAK ALTLRIHDSL NNSLPIGLVP TNSTNTVMDQ KNLKMSTPGQ MKAQEIERTP PTNFKRTLEE SNSGPLMKKH RRNGLSRSSG AQPASLPTTS QRKNSVKLTM RRRLRGQKKI GNPLLHQHRK TVCVC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

We are interested in CAT# CSB-EP018491HU, 0.5mg.
I want to know if we can produce the protein PBS buffer +50% glycerol.
Can we guarantee this or no?

A:
Thanks for your inquiry.
1. We can provide Aseptic Processing and Endotoxin Removal sevices for free, pls remark your requirements when placing the order.
2.Regarding your request about the buffer, we can try to use your desired buffer: PBS buffer +50% glycerol, but we are not sure if the protein will be stable under this buffer.
If it's finally proved to be not stable, we will communicate with you in advance and use our default buffer: Tris-based buffer,50% glycerol.

靶点详情

  • 功能：
    Involved in the negative regulation of p53 expression. Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. Mediates MAPK14 dephosphorylation and inactivation. Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity.
  • 基因功能参考文献：
    1. The observations suggest a novel role of ATR kinase in mediating its own signal attenuation via PPM1D recruitment to chromatin as an essential mechanism for restarting the stalled forks, cell cycle re-entry and cellular recovery from replication stress. PMID: 29485113
    2. NF-kappaB served as a positive transcriptional regulator of WIP1 to activate its expression and affect its function in colorectal cancer cells. PMID: 29367109
    3. ese results enhance our understanding of the molecular mechanisms that govern nucleoli formation by demonstrating that PPM1D regulates nucleolar formation by regulating NPM phosphorylation status through a novel signalling pathway, PPM1D-CDC25C-CDK1-PLK1 PMID: 27619510
    4. These data support the notion that Wip1 contributes to the formation of the ERMs in PDR membranes via NF-kappaB signaling. PMID: 28402943
    5. In an in vitro model of the blood-brain barrier, LPS raised Wip1 expression. Wip1 knockdown increased permeability and decreased transepithelial electrical resistance, protein expression of ZO-1, and occluding. Wip1 silencing augmented TNF-alpha, IL-1beta, IL-12, and IL-6. Sonic hedgehog signaling was activated by Wip1 overexpression and inhibited by silencing. Wip1 may protect the BBB against LPS damage via SHH signal... PMID: 29128669
    6. Studies suugest Wip1 role in tumorigenesis through regulation of p53 and p38MAPK pathways. PMID: 26883196
    7. The inhibition of Wip1 might fortify p53-mediated tumor suppression by Mdm2 antagonists. PMID: 27183917
    8. Wip1 suppressed ovarian cancer cell invasion, migration, epithelial to mesenchymal transition (EMT), and ovarian cancer metastasis in xenograft animal models. PMID: 27121065
    9. model reproduces the observed cellular phenotypes in experiments: oscillatory (for low DNA damage) regulated by negative feedback loops involving mainly p53 and Mdm2 and apoptotic or senescent (for high DNA damage) regulated by the positive p53/Wip1/miR-16 feedback loop PMID: 28968438
    10. This suggests an important cross-talk between SHH and WIP1 pathways that accelerates tumorigenesis and supports WIP1 inhibition as a potential treatment strategy for MB. PMID: 27086929
    11. Wip1 activity and its relevance to cancer as an oncoprotein is reviewed PMID: 28385459
    12. The s show that a global spread of ATM activity on chromatin and phosphorylation of ATM targets including KAP1 control Plk1 re-activation. These phosphorylations are rapidly counteracted by the chromatin-bound phosphatase Wip1, allowing cell cycle restart despite persistent ATM activity present at DNA lesions. PMID: 28607002
    13. Truncating and missense PPM1D mutations are associated with prostate cancer. PMID: 27401275
    14. this study shows that expression of Wip1 is decreased in patients with active inflammatory bowel disease PMID: 27687530
    15. Wip1 is frequently overexpressed in nonsmall cell lung cancer, which may serve an essential role in the p38MAPK/p53/p16 signaling pathway. PMID: 27959454
    16. Study identified 14 individuals with mild to severe Intellectual Disability (ID) and/or developmental delay and de novo truncating PPM1D mutations; all mutations were located in the last or penultimate exon, suggesting escape from nonsense-mediated mRNA decay. PMID: 28343630
    17. PPM1D gene silencing combined with TMZ eradicates glioma cells through cell apoptosis and cell cycle arrest PMID: 27633132
    18. High PPM1D expression is associated with breast cancer. PMID: 27485113
    19. PPM1D in the chromosomal location 17q22-24 might be the most relevant candidate gene with respect to a potential functional implication in meningioma progression. PMID: 26942600
    20. potential prognostic marker and therapeutic target for hepatocellular carcinoma PMID: 26809466
    21. PPM1D suppresses pancreatic cancer cell apoptosis via inhibition of the p38 MAPK/p53 pathway through both dephosphorylation of p38 MAPK and downregulation of ASPP2. PMID: 26714478
    22. squalene inhibits the ATM-dependent signaling pathway following DNA damage through intracellular induction of Wip1 expression. PMID: 26824362
    23. Chemotherapy exposure and age influence the accumulation of PPM1D mutations in the peripheral blood mononuclear cells of ovarian cancer patients. PMID: 26847329
    24. Knockdown of Wip1 enhances sensitivity to radiation in HeLa cells by inhibiting cell proliferation and inducing apoptosis through activation of p38 MAPK. PMID: 26351212
    25. Results showed evidence that Wip1 underwent Cdh1-dependent proteolysis during mitosis and sustained Wip1 activity during mitosis, resulting in mitotic delay at the metaphase to anaphase transition. PMID: 25649870
    26. Study reveals a mechanism of stem cell aging, in which distinct effects of p53 and mTORC1 pathways on HSC aging are governed by Wip1. PMID: 25879755
    27. WIP1 silencing reduced expression of MMP-9, VEGF-C, cyclin D1, and c-Myc, as well as migration and invasion of salivary adenoid cystic carcinoma cells. WIP1 expression was positively associated with ACC metastasis and prognosis. PMID: 25797250
    28. Wip1 may be involved in the biological processes of nasopharyngeal cancer cell proliferation, apoptosis, and migration and invasion. PMID: 25060857
    29. p21 might contribute to positive regulation of WIP1, resulting in dephosphorylation of the histone H2A variant gammaH2AX. PMID: 26006237
    30. PPM1D plays an important role in promoting CDDP resistance. PMID: 25154814
    31. data suggests that WIP1 inhibition represents a novel therapeutic approach to neuroblastoma that could be integrated with current chemotherapeutic approaches PMID: 25658463
    32. ATM kinase and Wip1 phosphatase were identified as opposing regulators of DAXX-S564 phosphorylation and the role of DAXX phosphorylation and DAXX itself are independent of p53-mediated gene expression. PMID: 25659035
    33. Wip1 protein was increased in kidney carcinoma. PMID: 24970693
    34. Results show that downregulation of HIPK2 increases resistance of bladder cancer cell to cisplatin by regulating Wip1. PMID: 24846322
    35. PPM1D plays an important role in the progression of NSCLC. PMID: 25412952
    36. High PPM1D expression is associated with lung cancer. PMID: 25123458
    37. The truncating mutations of PPM1D are present in blood DNA of NSCLC patients. PMID: 25742468
    38. Results demonstrate increased WIP1 expression in metastatic medulloblastomas, and inferior survival in patients with WIP1 high-expressing medulloblastoma. PMID: 24632620
    39. WIP1 knockdown inhibited the invasiveness of CNE-2 and 5-8F cells and was associated with the down-regulation of the expression of matrix metallopeptidase 9 PMID: 24801909
    40. Gain-of-function PPM1D mutations are associated with brainstem gliomas. PMID: 24880341
    41. High PPM1D expression is associated with prostate cancer. PMID: 24922655
    42. PP4 and Wip1 are differentially required to counteract the p53-dependent cell cycle arrest in G1 and G2, by antagonizing early or late p53-mediated responses, respectively PMID: 24711418
    43. Wip1 may be involved in the biological processes of non small cell lung cancer cell proliferation, cell apoptosis, and cell cycle. PMID: 24272082
    44. PPM1D plays a crucial role in colorectal carcinoma cell proliferation and colony formation. PMID: 23745790
    45. PPM1D mutations in the mosaic state predispose women to breast and ovarian cancer in the absence of a family history of cancer PMID: 24262437
    46. Expression of Wip1 in neutrophils was downregulated quickly after neutrophil activation through JNK-microRNA-16 pathway. It was negatively correlated with inflammatory cytokine productions of neutrophils in sepsis patients. PMID: 24395919
    47. Genetic variants and mutations of PPM1D control the response to DNA damage. PMID: 23907125
    48. BAX dephosphorylation of Wip1 phosphatase is an important regulator of resistance to anticancer therapy. PMID: 23907458
    49. High WIP1 expression is associated with colorectal cancer. PMID: 23679302
    50. Wip1 plays an important role in the regulation of global heterochromatin silencing and thus is critical in maintaining genome integrity PMID: 24135283

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  • 相关疾病：
    Intellectual developmental disorder with gastrointestinal difficulties and high pain threshold (IDDGIP); Breast cancer (BC); Ovarian cancer (OC)
  • 亚细胞定位：
    Nucleus. Cytoplasm, cytosol.
  • 蛋白家族：
    PP2C family
  • 组织特异性：
    Expressed in fetal and adult brain. Also detected in fetal liver and skeletal muscle, but not in their adult counterparts.
  • 数据库链接：

    HGNC: 9277

    OMIM: 114480

    KEGG: hsa:8493

    STRING: 9606.ENSP00000306682

    UniGene: Hs.286073