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  4. Recombinant Human Protein numb homolog (NUMB)

Recombinant Human Protein numb homolog (NUMB)

  • 货号：
    CSB-YP016186HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP016186HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP016186HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP016186HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP016186HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    c14_5527; C14orf41; FLJ31314; h Numb; h-Numb; Numb; Numb homolog (Drosophila); Numb homolog; Numb protein homolog; NUMB_HUMAN; Protein numb homolog; Protein S171; S171
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-651
  • 氨基酸序列
    MNKLRQSFRR KKDVYVPEAS RPHQWQTDEE GVRTGKCSFP VKYLGHVEVD ESRGMHICED AVKRLKAERK FFKGFFGKTG KKAVKAVLWV SADGLRVVDE KTKDLIVDQT IEKVSFCAPD RNFDRAFSYI CRDGTTRRWI CHCFMAVKDT GERLSHAVGC AFAACLERKQ KREKECGVTA TFDASRTTFT REGSFRVTTA TEQAEREEIM KQMQDAKKAE TDKIVVGSSV APGNTAPSPS SPTSPTSDAT TSLEMNNPHA IPRRHAPIEQ LARQGSFRGF PALSQKMSPF KRQLSLRINE LPSTMQRKTD FPIKNAVPEV EGEAESISSL CSQITNAFST PEDPFSSAPM TKPVTVVAPQ SPTFQANGTD SAFHVLAKPA HTALAPVAMP VRETNPWAHA PDAANKEIAA TCSGTEWGQS SGAASPGLFQ AGHRRTPSEA DRWLEEVSKS VRAQQPQASA APLQPVLQPP PPTAISQPAS PFQGNAFLTS QPVPVGVVPA LQPAFVPAQS YPVANGMPYP APNVPVVGIT PSQMVANVFG TAGHPQAAHP HQSPSLVRQQ TFPHYEASSA TTSPFFKPPA QHLNGSAAFN GVDDGRLASA DRHTEVPTGT CPVDPFEAQW AALENKSKQR TNPSPTNPFS SDLQKTFEIE L
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Regulates clathrin-mediated receptor endocytosis. Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage.
  • 基因功能参考文献：
    1. The Numb-Mdm2 interaction represents a fuzzy complex mediated by a short Numb sequence encompassing its alternatively spliced exon 3 (Ex3), which is necessary and sufficient to inhibit Mdm2 and prevent p53 degradation. PMID: 29269425
    2. High NUMB expression is associated with endometriosis. PMID: 29398419
    3. The results provide new evidence of Numb and Gli1 on the clinical characteristics of Malignant pleural mesothelioma, which may be helpful in clinical diagnosis and targeted therapy. Further research with larger sample size is needed PMID: 30197333
    4. Numb is associated with modulation of Notch-driven epithelial-mesenchymal transition. PMID: 29187638
    5. Numb(-/low) prostate cancer cells were smaller and quiescent, preferentially expressed Notch and Hedgehog downstream and stem-cell-associated genes, and associated with a greater resistance to androgen-deprivation therapy PMID: 28751447
    6. MAP17 overexpression activates Notch pathway by sequestering NUMB. High levels of MAP17 correlated with tumorsphere formation and Notch and Stem gene transcription. Its direct modification causes direct alteration of tumorsphere number and Notch and Stem pathway transcription PMID: 28153862
    7. NUMB has a role in negatively regulating the epithelial-mesenchymal transition of triple-negative breast cancer by antagonizing Notch signaling PMID: 27506933
    8. In the present study, we identified that the adaptor protein Numb, which is demonstrated to be a novel binding partner of NEDD4-1, plays important roles in controlling PTEN ubiquitination through regulating NEDD4-1 activity and the association between PTEN and NEDD4-1. PMID: 28437168
    9. Numb expression is not associated with favorable prognosis in small cell lung cancer. PMID: 28060745
    10. Data suggest that over-expression of NUMB has anti-cancer effects to prostatic cancer cells; these studies included experiments both in vitro and in vivo (xenograft experiments in nude mice). PMID: 28531799
    11. Using patient-derived xenografts, the study shows that expansion of the cancer stem cells pool, due to altered self-renewing divisions, is also a feature of Numb-deficient human breast cancers . PMID: 28298340
    12. Numb binds to another docking regulator, Mon1b, and is required for the recruitment of cytosolic Mon1b to the early endosomes membrane. PMID: 26987402
    13. this study identified a novel molecular determinant of breast cancer progression, uncovering a potential oncogenic role for the NUMB6 protein in cancer cell migration and invasion, coupled to the maintenance of mesenchymal-like cells. PMID: 27302072
    14. Low NUMB expression is associated with pancreatic cancer. PMID: 27092875
    15. Together, our findings revealed a novel function of Numb and its likely mechanism in regulation of autophagy events. PMID: 28720501
    16. investigations revealed that NUMB and NUMBL interacted with small GTPase Rab7 to transition ERBB2 from early to late endosome for degradation. PMID: 28067668
    17. These findings highlight the importance of Numb and Numbl in the control of myoepithelial cell fate determination, epithelial identity, and lactogenesis PMID: 27383182
    18. Our evidence supports a mechanism by which Numb AS is regulated in response to oncogenic signaling pathways, and contributes to activation of downstream pathways to promote tumorigenesis. PMID: 27041567
    19. Numb is a pivotal adaptor protein that mediates the subcellular localization of EAAT3 through binding the YxNxxF (where x stands for any amino acid) motif. PMID: 27358480
    20. HBeAg and its precursors promote HDM2-mediated degradation and impair transcriptional activity of p53 by interacting with NUMB, consequently contributing to hepatocellular carcinoma development. PMID: 27106262
    21. High expression of Numb is associated with radiation resistance in pancreatic cancer. PMID: 27677287
    22. Upregulated expression of Numb is associated with reduced radiosensitivity of lung cancer. PMID: 27476167
    23. Radiation sensitivity of NSCLC stem cells was enhanced with the increase of Numb expression. PMID: 27310305
    24. PNA-antimiR-182 reduced the levels of NICD, Hes1, HIF-1alpha, and p-Akt in both cell groups, while it augmented the intracellular content of FOXO1 and Numb suppressor proteins. In other words, PNA-antimiR-182-mediated upregulation of Numb was associated with downregulation of HIF-1alpha and Hes1. Consequently, downregulation of miR-182 might find therapeutical value for overcoming trastuzumab resistance PMID: 26563369
    25. Dominant negative NUMB expression confers a high proliferative phenotype to chronic myeloid leukemia cells. PMID: 26944313
    26. Notch1/Numb/Snail signal pathway plays an important role in the pathogenesis of adenomyosis. PMID: 26307032
    27. Numb polymorphisms are associated with coronary artery disease among Han Chinese and Uighur Chinese in Xinjiang, China. PMID: 26415596
    28. Methylation of the Numb promoter contributed to hepatocarcinogenesis. PMID: 26165304
    29. results indicated that both rs2108552 and rs17781919 in the Numb gene were associated with total cholesterol level and density lipoprotein-cholesterol level in Chinese subjects. PMID: 26264115
    30. Report alternative splicing of NUMB in colorectal cancer. PMID: 26074693
    31. NANOG-NUMB-p53 signaling axis is an important regulatory pathway for tumor-initiating cells events in TIC self-renewal and liver tumorigenesis PMID: 26174965
    32. Alternative splicing of Numb may provide a useful prognostic biomarker in HCC and is pharmacologically tractable PMID: 26058814
    33. Numb/Numbl control VEGF receptor endocytosis, signaling, and recycling in endothelial cells, which promotes the angiogenic growth of blood vessels. PMID: 26069237
    34. Using LC-MS/MS, the study reports the identification of 25 serine/threonine Numb phosphorylation sites, and a single tyrosine phosphorylation site. PMID: 25403733
    35. Taken together, our data suggest that Numb may possibly function as a tumor suppressor involved in the carcinogenesis of clear cell renal cell carcinoma PMID: 25480416
    36. Findings suggested NUMB-1 functions as a tumor-suppressor and serves as a prognositc biomarker for ESCC patients. PMID: 24980814
    37. Numb specifically regulates NPC1L1-mediated cholesterol absorption both in human intestine and liver, distinct from ARH and Dab2, which selectively participate in LDLR-mediated LDL uptake. PMID: 25331956
    38. Numb up-regulation significantly correlates with cell proliferation and poor prognosis in hepatocellular carcinoma patients. PMID: 24770339
    39. miR-146a enhances the oncogenicity of oral carcinoma by concomitant targeting of the IRAK1, TRAF6 and NUMB genes. PMID: 24302991
    40. The study demonstrates coordinated regulation of Numb, MDM2 and p53 on cell invasion and migration in pancreatic cancer. PMID: 23881403
    41. Identify the Notch pathway regulator NUMB as a key target of these factors. PMID: 24332178
    42. Overexpression of Numb inhibited proliferation, promoted apoptosis and enhanced sensitivity to cisplatin, and activated caspase-9 and caspase-3 through release of cytochrome c as well as downregulation of XIAP and survivin. PMID: 23624653
    43. The Numb modulates the paracellular permeability by affecting apical junctional complex (AJC) assembly and myosin light chain (MLC) phosphorylation. PMID: 23872314
    44. Numb1 interacts with TRPV6 through charged residues and inhibits its activity via the regulation of protein expression. PMID: 23140583
    45. Data indicate the Set8-Numb-p53 signaling axis as an important regulatory pathway for apoptosis and suggests a therapeutic strategy by targeting Numb methylation. PMID: 23706821
    46. This study implies that any mutations within the NUMB gene that might influence the disease course of chronic myeloid leukemia are very rare. PMID: 22734830
    47. Expression of stem cell regulator NUMB is not associated with any prognostic significance in acute myeloid leukemia patients. PMID: 23233047
    48. Down-modulation of NUMB is associated with myelodysplastic syndromes and acute myeloid leukemia. PMID: 22784712
    49. Dysregulation of Numb expression results in mislocalized Plk1 and poor centrosomal gamma-tubulin recruitment, potentially contributing to mitotic errors, aneuploidy, and cancer development. PMID: 22593191
    50. Numb regulates glioma stem cell fate and growth by altering epidermal growth factor receptor and Skp1-Cullin-F-box ubiquitin ligase activity PMID: 22553175

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  • 亚细胞定位：
    Cell membrane; Peripheral membrane protein; Cytoplasmic side. Endosome membrane; Peripheral membrane protein; Cytoplasmic side.
  • 数据库链接：

    HGNC: 8060

    OMIM: 603728

    KEGG: hsa:8650

    STRING: 9606.ENSP00000347169

    UniGene: Hs.525443