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  4. Recombinant Human Protein enabled homolog (ENAH)

Recombinant Human Protein enabled homolog (ENAH)

  • 货号：
    CSB-YP854072HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP854072HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP854072HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP854072HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP854072HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    ENA; Enah; ENAH_HUMAN; MENA; NDPP1; Protein enabled homolog
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-591
  • 氨基酸序列
    MSEQSICQAR AAVMVYDDAN KKWVPAGGST GFSRVHIYHH TGNNTFRVVG RKIQDHQVVI NCAIPKGLKY NQATQTFHQW RDARQVYGLN FGSKEDANVF ASAMMHALEV LNSQETGPTL PRQNSQLPAQ VQNGPSQEEL EIQRRQLQEQ QRQKELERER LERERMERER LERERLERER LERERLEQEQ LERERQERER QERLERQERL ERQERLERQE RLDRERQERQ ERERLERLER ERQERERQEQ LEREQLEWER ERRISSAAAP ASVETPLNSV LGDSSASEPG LQAASQPAET PSQQGIVLGP LAPPPPPPLP PGPAQASVAL PPPPGPPPPP PLPSTGPPPP PPPPPLPNQV PPPPPPPPAP PLPASGFFLA SMSEDNRPLT GLAAAIAGAK LRKVSRMEDT SFPSGGNAIG VNSASSKTDT GRGNGPLPLG GSGLMEEMSA LLARRRRIAE KGSTIETEQK EDKGEDSEPV TSKASSTSTP EPTRKPWERT NTMNGSKSPV ISRRDSPRKN QIVFDNRSYD SLHRPKSTPL SQPSANGVQT EGLDYDRLKQ DILDEMRKEL TKLKEELIDA IRQELSKSNT A
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation.
  • 基因功能参考文献：
    1. Endothelin-1 is a regulator of Mena expression in the serous ovarian cancer.Mena expression is associated with a poor prognosis in serous ovarian cancer patients.Mena is recruited to mature invadopodia in endothelin-1 and beta-arrestin-1 signaling. PMID: 29439204
    2. the difference between mRNAs encoding constitutive Mena sequences and those containing the 11a exon correlates with metastasis in colorectal cancer, suggesting that 11a exon exclusion contributes to invasive phenotypes and leads to poor clinical outcome PMID: 27748415
    3. Mena(INV) promotes invadopodium maturation by inhibiting normal dephosphorylation of cortactin at tyrosine 421 by the phosphatase PTP1B. PMID: 27824079
    4. SHIP2 recruits Mena to invadopodia and disruption of SHIP2-Mena interaction in cancer cells leads to attenuated capacity for ECM degradation and invasion in vitro, as well as reduced metastasis in vivo. PMID: 27597754
    5. PPARgamma might inhibit the proliferation and migration of GC cell lines through suppressing the expression of TERT and ENAH PMID: 26956036
    6. our results indicate that hMENA11a is an anti-apoptotic regulator involved in the HER3-mediated mechanisms of resistance to PI3K inhibition in breast cancer PMID: 25961924
    7. these data provide the first description of endogenous Mena(INV) protein expression in mouse and human breast tumors. PMID: 26680363
    8. we evaluate the prognostic value of Menacalc in a cohort of ANN patients. Our primary objective was to determine if there was an association between Menacalc and overall patient mortality. PMID: 26112005
    9. High Menacalc levels identify a subgroup of breast cancer patients with poor disease-specific survival, suggesting that Menacalc may serve as a biomarker for metastasis. PMID: 22971274
    10. Invasive breast ductal-carcinoma cells that migrated thru a layer of human endothelial cells were enriched for the transcript encoding Mena(INV), an invasive isoform of Mena. PMID: 25429076
    11. the higher relative expression of hMena(11a) compared with hMena(INV) may predict malignant transformation in breast epithelial cells, and, furthermore, a reversal of expression of hMena(11a) and hMena(INV) may dictate the state of cancer progression PMID: 25109497
    12. High ENAH expression is associated with trastuzumab-resistant breast cancer. PMID: 25449779
    13. determined the alpha-synuclein-binding domain of beta-III tubulin and demonstrated that a short fragment containing this domain can suppress alpha-synuclein accumulation in the primary cultured cells PMID: 25031323
    14. High expression of Mena is associated with hepatocellular carcinoma. PMID: 24683008
    15. we consider that it is necessary to analyze the expression of Mena splice variants in a higher number of cases, in different epithelial lesions, and also in experimental studies to define its exact role in carcinogenesis PMID: 23956979
    16. this study suggests a novel mechanism in which Lpd mediates EGFR endocytosis via Mena downstream of endophilin. PMID: 24076656
    17. This study demonistrated that dysregulation of ENAH eeox in brain of patient with schizophrenia. PMID: 23062752
    18. These data suggest that polarized and growth-arrested cellular architecture correlates with absence of alternative hMENA isoform expression. PMID: 23129656
    19. Overexpression of Wnt1 and Wnt3a ligands increased MENA mRNA levels. PMID: 22615875
    20. Mena protein seems to play a role in malignant transformation and its intensity is correlated with the type and grade of tumor and also with vascular invasion. Its positivity in endothelial cells may suggest its potential role in tumor angiogenesis. PMID: 22472896
    21. results indicate that by mediating intensive F-actin accumulation at the sites of cell infiltration, WAVE2, N-WASP, and Mena are crucial for PI3K-dependent cell invasion induced by PDGF PMID: 21769917
    22. ENA/VASP-family proteins are functionally redundant in homologous recombination, and MENA, VASP and EVL may be involved in the DSB repair pathway in humans PMID: 21398369
    23. Data show that VASP and Mena interact with RSK1. PMID: 21423205
    24. hMena and HER2 signalling has a role in breast cancer PMID: 21209853
    25. Mammalian enabled (Mena) is a critical regulator of cardiac function. PMID: 21335464
    26. ENAH polymorphisms are associated with increased susceptibility, development of proteinuria and gross hematuria, and pathological progression of childhood IgAN. PMID: 19641378
    27. mena phosphorylation is regulated by Abi-1 and c-Abl PMID: 12672821
    28. overexpression of ENAH is associated with breast cancer PMID: 16533770
    29. Oservations identify Tes as an atypical binding partner of EVH1 domain of Mena and a regulator specific to a single Ena/VASP family member. PMID: 18158903
    30. the hMena(+11a) isoform is associated with an epithelial phenotype and identifies EGFR-dependent cell lines that are sensitive to the EGFR inhibitor erlotinib PMID: 18676769
    31. two isoforms of Mena, ++ and +++, are upregulated in the invasive tumor cells and one isoform, 11a, is downregulated PMID: 18985426
    32. the Mena invasion isoform potentiates EGF-induced carcinoma cell invasion and metastasis PMID: 19081071
    33. Increased hMena expression is associated with colorectal carcinomas. PMID: 19082477
    34. hMena possesses properties which help to regulate the formation of lamellipodia through the modulation of the activity of Rac1. PMID: 19277120
    35. Mena was not expressed in the normal cervical squamous epithelium but its expression was increased in parallel with the increasing grade of CIN, with up-regulation upon transition to CIN3 and further to invasive carcinoma. PMID: 19434313

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, filopodium. Cell junction, synapse. Cell junction, focal adhesion.
  • 蛋白家族：
    Ena/VASP family
  • 组织特异性：
    Expressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate fibromuscolar stroma and placenta vascular media.
  • 数据库链接：

    HGNC: 18271

    OMIM: 609061

    KEGG: hsa:55740

    STRING: 9606.ENSP00000355809

    UniGene: Hs.497893