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  4. Recombinant Human Protein arginine N-methyltransferase 6 (PRMT6)

Recombinant Human Protein arginine N-methyltransferase 6 (PRMT6)

  • 中文名称：
    人PRMT6重组蛋白
  • 货号：
    CSB-YP856972HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人PRMT6重组蛋白
  • 货号：
    CSB-EP856972HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人PRMT6重组蛋白
  • 货号：
    CSB-EP856972HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人PRMT6重组蛋白
  • 货号：
    CSB-BP856972HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人PRMT6重组蛋白
  • 货号：
    CSB-MP856972HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PRMT6
  • Uniprot No.：
  • 别名：
    ANM6_HUMAN; Chromobox protein homolog 7; FLJ10559; FLJ51477; Heterogeneous nuclear ribonucleoprotein methyltransferase like protein 6; Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6; Histone-arginine N-methyltransferase PRMT6; HMT1 hnRNP methyltransferase like 6.; HRMT1L6; OTTHUMP00000012633; PRMT 6; prmt6; Protein arginine methyltransferase 6; Protein arginine N methyltransferase 6; Protein arginine N-methyltransferase 6
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-375
  • 氨基酸序列
    MSQPKKRKLE SGGGGEGGEG TEEEDGAERE AALERPRRTK RERDQLYYEC YSDVSVHEEM IADRVRTDAY RLGILRNWAA LRGKTVLDVG AGTGILSIFC AQAGARRVYA VEASAIWQQA REVVRFNGLE DRVHVLPGPV ETVELPEQVD AIVSEWMGYG LLHESMLSSV LHARTKWLKE GGLLLPASAE LFIAPISDQM LEWRLGFWSQ VKQHYGVDMS CLEGFATRCL MGHSEIVVQG LSGEDVLARP QRFAQLELSR AGLEQELEAG VGGRFRCSCY GSAPMHGFAI WFQVTFPGGE SEKPLVLSTS PFHPATHWKQ ALLYLNEPVQ VEQDTDVSGE ITLLPSRDNP RRLRVLLRYK VGDQEEKTKD FAMED
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Arginine methyltransferase that can catalyze the formation of both omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA. Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates. Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a. H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). Acts as a transcriptional repressor of various genes such as HOXA2, THBS1 and TP53. Repression of TP53 blocks cellular senescence. Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to the stimulation of its polymerase activity by enhancing DNA binding and processivity. Methylates HMGA1. Regulates alternative splicing events. Acts as a transcriptional coactivator of a number of steroid hormone receptors including ESR1, ESR2, PGR and NR3C1. Promotes fasting-induced transcriptional activation of the gluconeogenic program through methylation of the CRTC2 transcription coactivator. May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC). Methylates GPS2, protecting GPS2 from ubiquitination and degradation. Methylates SIRT7, inhibiting SIRT7 histone deacetylase activity and promoting mitochondria biogenesis.
  • 基因功能参考文献：
    1. This study indicated that the hypomethylation of PRMT6 promoter could be a novel diagnostic biomarker for colorectal cancer. PMID: 29927001
    2. PRMT6 negatively regulates DNA methylation in cancer. PRMT6 overexpression impairs chromatin association of UHRF1, an accessory factor of DNMT1, resulting in passive DNA demethylation. PMID: 29262320
    3. Data suggest that histone-arginine N-methyltransferase PRMT6 (PRMT6) plays an oncogenic role in prostate cancer (PCa) and predicts for more clinically aggressive disease, constituting a potential target for patients with Prostate cancer (CRPC). PMID: 27323813
    4. Based on the structural information here and available in the PDB database, we proposed a mechanism that can rationalize the distinctive arginine methylation product specificity of different types of arginine methyltransferases and pinpoint the structural determinant of such a specificity PMID: 27480107
    5. PRMT6, which was down-regulated by androgen receptors and influenced cell migration and apoptosis of germ cells, could play a potentially important role in spermatogenesis. PMID: 26690413
    6. PRMT6 methylates tumor cell p21 at arginine 156 and promotes phosphorylation of threonine 145 on p21, increasing the cytoplasmic localization of p21 and inducing resistance to anticancer drugs. PMID: 26436589
    7. PRMT6 influences PRC-mediated gene silencing at the rostral HOXA genes locus. PMID: 26848759
    8. Data show that HIV-1 Tat methylation by protein arginine methyltransferase 6 (PRMT6) can inhibit nucleolar retention. PMID: 26611710
    9. N terminus is methylated by PRMT6 and that this critically affects the functions of pUL69 for efficient mRNA export and replication of human cytomegalovirus. PMID: 26178996
    10. TBL1 is required to protect GPS2 from degradation, with methylation of GPS2 by arginine methyltransferase PRMT6 regulating the interaction with TBL1 and inhibiting proteasome-dependent degradation. PMID: 26070566
    11. PRMT6 mediates cigarette smoke extract induced apoptosis and inflammation through H3R2me2a in HUVECs. PMID: 25481537
    12. PRMT6 specifically interacts with ERalpha at its ligand-binding domain. PMID: 24742914
    13. s show that PRMT6 requires the activation domain, but surprisingly not the basic domain, of HIV-1 Tat for protein interaction. PMID: 23800116
    14. Data show arginine methyltransferase PRMT6 methylates the AT-hook motif of regulatory factor RFX5 and downregulates HLA-DQ expression. PMID: 23911394
    15. Arginine 42 of histone H3 (H3R42), is dimethylated by the methyltransferases coactivator arginine methyltransferase 1 (CARM1) and protein arginine methyltransferase 6 (PRMT6) in vitro and in vivo. PMID: 23980157
    16. these data demonstrate that PRMT6 overexpression is associated with regulation of motility and invasion through up-regulation of TSP-1 and down-regulation of MMPs in human cancer cells. PMID: 23380452
    17. These results show that PRMT6 automethylation plays a role in the stability of this protein and that this event is indispensible for its anti-HIV-1 activity. PMID: 23866860
    18. PRMT6 is recruited by RUNX1 and mediates asymmetric histone H3 arginine-2 dimethylation (H3R2me2a) at megakaryocytic genes in progenitor cells. PMID: 22777353
    19. 36 new putative partners for PRMT6 were identified. PMID: 23326497
    20. Findings indicate that expression of PRMT4/CARM1 and PRMT6 is deregulated in melanoma. PMID: 23265702
    21. Gene expression and promoter analysis show that p21 and p27 are direct targets of PRMT6, which involves methylation of arginine-2 of histone H3. Our findings imply arginine methylation of histones by PRMT6 in cell cycle regulation PMID: 22916108
    22. Specific arginine residues of p16 protein are methylated by PRMT6 which may be critical for the activity of p16. PMID: 23032699
    23. dysregulation of PRMT6-dependent transcription and alternative splicing may be involved in breast cancer pathophysiology and the molecular consequences identifying a unique and informative biomarker profile. PMID: 22673335
    24. the molecular mechanisms of PRMT catalysis PMID: 22219200
    25. In this study, we use Forster resonance energy transfer (FRET) to determine dissociation constant (K(D)) values for dimerization of PRMT1 and PRMT6. PMID: 20812326
    26. PRMT6 may be involved in human carcinogenesis and may thus be a therapeutic target for various types of cancer. PMID: 20473859
    27. methylates HIV-1 Tat protein and acts as a restriction factor for HIV-1 replication PMID: 15596808
    28. severely impairs the function of HIV-1 Rev binding to and export of viral RNA PMID: 17176473
    29. findings show HIV-1 Tat is a specific substrate of PRMT6 which targets Tat R52 & R53 residues for arginine methylation; PRMT6 can compromise Tat transcriptional activation & may represent a form of innate cellular immunity in regard to HIV-1 replication PMID: 17267505
    30. the arginine methyltransferase PRMT6 catalyses H3R2 di-methylation in vitro and controls global levels of H3R2me2a in vivo PMID: 17898714
    31. PRMT6 methylates H3R2 and is a negative regulator of N-terminal H3 tail binding PMID: 18077460
    32. findings identify PRMT6 as the mammalian methyltransferase for H3 R2 and establish the enzyme as a crucial negative regulator of H3 K4 trimethylation and transcriptional activation PMID: 18079182
    33. TSP-1 is a transcriptional repression target of PRMT6 PMID: 19509293

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family, PRMT6 subfamily
  • 组织特异性：
    Highly expressed in kidney and testis.
  • 数据库链接：

    HGNC: 18241

    OMIM: 608274

    KEGG: hsa:55170

    STRING: 9606.ENSP00000359095

    UniGene: Hs.26006