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  4. Recombinant Human Protein NEDD1 (NEDD1)

Recombinant Human Protein NEDD1 (NEDD1)

  • 中文名称：
    人NEDD1重组蛋白
  • 货号：
    CSB-YP822820HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人NEDD1重组蛋白
  • 货号：
    CSB-EP822820HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人NEDD1重组蛋白
  • 货号：
    CSB-EP822820HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人NEDD1重组蛋白
  • 货号：
    CSB-BP822820HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人NEDD1重组蛋白
  • 货号：
    CSB-MP822820HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NEDD1
  • Uniprot No.：
  • 别名：
    FLJ35902; GCP WD; NEDD-1; NEDD1; NEDD1_HUMAN; Neural precursor cell expressed developmentally down-regulated protein 1; Neural precursor cell expressed, developmentally down regulated gene 1; Protein NEDD1; TUBGCP7
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-660
  • 氨基酸序列
    MQENLRFASS GDDIKIWDAS SMTLVDKFNP HTSPHGISSI CWSSNNNFLV TASSSGDKIV VSSCKCKPVP LLELAEGQKQ TCVNLNSTSM YLVSGGLNNT VNIWDLKSKR VHRSLKDHKD QVTCVTYNWN DCYIASGSLS GEIILHSVTT NLSSTPFGHG SNQSVRHLKY SLFKKSLLGS VSDNGIVTLW DVNSQSPYHN FDSVHKAPAS GICFSPVNEL LFVTIGLDKR IILYDTSSKK LVKTLVADTP LTAVDFMPDG ATLAIGSSRG KIYQYDLRML KSPVKTISAH KTSVQCIAFQ YSTVLTKSSL NKGCSNKPTT VNKRSVNVNA ASGGVQNSGI VREAPATSIA TVLPQPMTSA MGKGTVAVQE KAGLPRSINT DTLSKETDSG KNQDFSSFDD TGKSSLGDMF SPIRDDAVVN KGSDESIGKG DGFDFLPQLN SVFPPRKNPV TSSTSVLHSS PLNVFMGSPG KEENENRDLT AESKKIYMGK QESKDSFKQL AKLVTSGAES GNLNTSPSSN QTRNSEKFEK PENEIEAQLI CEPPINGSST PNPKIASSVT AGVASSLSEK IADSIGNNRQ NAPLTSIQIR FIQNMIQETL DDFREACHRD IVNLQVEMIK QFHMQLNEMH SLLERYSVNE GLVAEIERLR EENKRLRAHF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for mitosis progression. Promotes the nucleation of microtubules from the spindle.
  • 基因功能参考文献：
    1. One important function of Aurora A in mitotic cells is to promote microtubule nucleation around the chromatin by phosphorylating NEDD1, and thereby to promote functional spindle assembly. PMID: 23273898
    2. The mice treated with small interfering (si) RNA targeting NEDD1 by the atelocollagen-mediated delivery system showed a significantly prolonged survival in a xenograft model of human gastric cancer. PMID: 23106787
    3. Data suggest that the NEDD1/gamma-tubulin interaction is finely tuned by multiple phosphorylation events in the S557-S574 region and is critical for spindle assembly. PMID: 22595525
    4. a direct interaction with NEDD1 regulates gamma-tubulin recruitment to the centrosome PMID: 20224777
    5. GCP-WD (NEDD1) broadly mediates targeting of the gamma-tubulin ring complex to sites of microtubule nucleation and to the mitotic spindle, which is essential for spindle formation. PMID: 16378099
    6. The NEED1 protein was characterized and its function in centriole duplication and spindle assembly determined. PMID: 16461362
    7. Data propose NEDD1 may be a promising target for controlling cell proliferation, in particular if targeted in combination with Plk1 inhibitors. PMID: 19243593
    8. Data suggest that sequential phosphorylation of Nedd1 by Cdk1 and Plk1 plays a role in targeting gamma tubulin ring complex to the centrosome by promoting the interaction of Nedd1 with the gammaTuRC component gamma-tubulin, during mitosis. PMID: 19509060

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 数据库链接：

    HGNC: 7723

    OMIM: 600372

    KEGG: hsa:121441

    STRING: 9606.ENSP00000451211

    UniGene: Hs.270084