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  4. Recombinant Human Protein HEXIM1 (HEXIM1)

Recombinant Human Protein HEXIM1 (HEXIM1)

  • 货号：
    CSB-YP010318HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP010318HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP010318HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP010318HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP010318HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    HEXIM1
  • Uniprot No.：
  • 别名：
    Cardiac lineage protein 1; CLP 1; CLP1; EDG 1; EDG1; Estrogen down-regulated gene 1 protein; FLJ13562; Hexamethylene bis acetamide inducible 1; Hexamethylene bis acetamide inducible protein; Hexamethylene bis acetamide inducible transcript 1; Hexamethylene bis-acetamide-inducible protein 1; HEXI1_HUMAN; HEXIM 1; Hexim1; HEXIM1 protein; HIS 1; HIS1; HMBA inducible ; MAQ 1; MAQ1; Menage a quatre 1; Menage a quatre protein 1; Protein HEXIM1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-359
  • 氨基酸序列
    MAEPFLSEYQ HQPQTSNCTG AAAVQEELNP ERPPGAEERV PEEDSRWQSR AFPQLGGRPG PEGEGSLESQ PPPLQTQACP ESSCLREGEK GQNGDDSSAG GDFPPPAEVE PTPEAELLAQ PCHDSEASKL GAPAAGGEEE WGQQQRQLGK KKHRRRPSKK KRHWKPYYKL TWEEKKKFDE KQSLRASRIR AEMFAKGQPV APYNTTQFLM DDHDQEEPDL KTGLYSKRAA AKSDDTSDDD FMEEGGEEDG GSDGMGGDGS EFLQRDFSET YERYHTESLQ NMSKQELIKE YLELEKCLSR MEDENNRLRL ESKRLGGDDA RVRELELELD RLRAENLQLL TENELHRQQE RAPLSKFGD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. Core component of the 7SK RNP complex: in cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.
  • 基因功能参考文献：
    1. The 7SK RNA binds to Hexim1 and turns it into an inhibitor of the positive transcription elongation factor (P-TEFb). And in addition to its action on P-TEFb, it plays a role in a variety of different mechanisms: it controls the stability of transcription factor components and assists binding of transcription factors to their targets. PMID: 29345523
    2. We conclude that the degradation of NPM1 and HEXIM1 through autophagy in certain AML subsets contributes to the activation of the BET pathway in these cells. PMID: 27732946
    3. Identify a HEXIM1-containing ribonuclear protein complex composed also of DNA-PK, paraspeckle subunits, and the long non-coding RNA (lncRNA) NEAT1, which acts as a key nuclear regulator of DNA-mediated activation of innate immune response through the cGAS-STING pathway. PMID: 28712728
    4. HMBA and derivative HMBA4a1 induce HEXIM1 activity, targeting several signaling pathways critical in tumorigenesis and metastasis. PMID: 27238569
    5. HSP70 activator shows similar activity as HMBA and 4a1 to induce HEXIM1 expression, suggesting that HMBA and 4a1 might be putative HSP70 activators PMID: 28213333
    6. Our results demonstrated that HEXIM1 exhibited the most consistent response to BET inhibitors in all of the settings examined, including tumors and surrogate tissues. PMID: 27903752
    7. Suggest that greater tumor associated macrophage density, strong Hexim1 expression, strong SMAD2 expression, and mild SMAD7 expression play important roles in the progression of prostate adenocarcinoma. PMID: 26608417
    8. Results show that HEXIM1 is a tumor suppressor in melanoma that responds to nucleotide stress by inhibiting transcription elongation of tumorigenic genes and stabilizing mRNA transcripts of other tumor suppressor genes. PMID: 27058786
    9. Data indicate the binding of RNA-binding protein HEXIM with 7SKsnRNP complex comprising the non-coding RNA 7SK and proteins MePCE and LARP7. PMID: 25863285
    10. PPM1G phosphatase directly binds 7SK RNA and the kinase inhibitor Hexim1 once P-TEFb has been released from the 7SK snRNP. PMID: 26324325
    11. This study demonstrates a novel role of HEXIM1 in regulating human pluripotent stem cells fate through a P-TEFb-independent pathway. PMID: 23977357
    12. HEXIM1 functions as an AR (androgen receptor) co-repressor as it physically interacts with the AR and is required for the ability of anti-androgens to inhibit androgen-induced target gene expression and cell proliferation. PMID: 24844355
    13. the release of P-TEFb from the 7SK snRNP led to increased synthesis of HEXIM1 but not HEXIM2 PMID: 24515107
    14. There is evidence for a direct interaction between HIF-1alpha and HEXIM1, and HEXIM1 up-regulated hydroxylation of HIF-1alpha. PMID: 24015760
    15. P-TEFb/HEXIM1-dependent transcriptional regulation may play a pathophysiological role in RVH and be a novel therapeutic target for mitigating RVH in PAH PMID: 23300697
    16. results not only identify HEXIM1 as a positive regulator of p53, but also propose a novel molecular mechanism of p53 activation caused by the anti-cancer drugs and compounds. PMID: 22948151
    17. Changes in Hexim-1 protein expression and cellular distribution significantly influence androgen receptor activation and transforming growth factor (TGF)-beta signaling in prostate cancer progression. PMID: 22095517
    18. N-terminal residues in cyclin T1 are specifically involved in the binding of cyclin T1 to HEXIM1 but not to Tat. PMID: 22342181
    19. Structural insights were provided into the recognition of the regulator protein Hexim1 by P-TEFb and the modulation of coiledcoil dynamics by specific discontinuities. PMID: 22033481
    20. Tat efficiently replaces HEXIM1 on the 7SK snRNA in vivo and it promotes the disassembly of the 7SK/HEXIM/P-TEFb negative transcriptional regulatory snRNP to augment the nuclear level of active P-TEFb. PMID: 20976203
    21. reincorporation of HEXIM1 into the 7SK snRNP is likely the regulated step of reassembly of the 7SK snRNP containing P-TEFb PMID: 20808803
    22. data provide evidence to suggest a novel role for HEXIM1 in cancer progression PMID: 20453883
    23. T-loop phosphorylated Cdk9 localizes to nuclear speckle domains which may serve as sites of active P-TEFb function and exchange between the Brd4 and 7SK/HEXIM1 regulatory complexes. PMID: 20201073
    24. binding to Cyclin T1 induces an asymmetry or sterical hindrance in the first coiled coil segment of dimeric Hexim1 that disallows formation of a 2:2 complex. PMID: 20210365
    25. MAQ1 cooperates with 7SK RNA to form a novel type of CDK inhibitor. PMID: 12832472
    26. Binding of the 7SK snRNA turns the HEXIM1 protein into a P-TEFB (CDK9/cyclin T) inhibitor. PMID: 15201869
    27. HEXIM1 and HEXIM2 exhibit distinct expression patterns in various human tissues PMID: 15713661
    28. utilization of HEXIM1 or HEXIM2 to bind and inhibit P-TEFb can be differentially regulated in vivo. PMID: 15713662
    29. Hexim1 has a cyclin T-binding domain and binds competitively to HIV-1 Tat PMID: 15855166
    30. increased estrogen down regulated gene 1 expression results in inhibition of cyclin T1 recruitment and estrogen receptor 1 DNA binding PMID: 15940264
    31. HEXIM1 forms a transcriptionally abortive complex with glucocorticoid receptor without involving 7SK RNA and positive transcription elongation factor b. PMID: 15941832
    32. HEXIM1 dimer associates with only one molecule of RNA. PMID: 15965233
    33. HIV-1 Tat transactivation is effectively inhibited by co-expression of HEXIM1; HEXIM1 expression specifically represses transcription mediated by the direct activation of P-TEFb through artificial recruitment of GAL4-CycT1 PMID: 15992410
    34. analysis of positive transcription elongation factor - HEXIM1 - 7SK RNA complex PMID: 15994294
    35. Ectopic expression of HEXIM1 causes growth inhibition and promotes neuronal differentiation. These findings highlight a crucial role of HEXIM1 protein during cell differentiation. PMID: 16222702
    36. the interplay between 7SK snRNA and oppositely charged regions in HEXIM1 direct its binding to P-TEFb and subcellular localization that culminates in the inhibition of transcription PMID: 16362050
    37. Binding of HEXIM1 is a prerequisite for association of P-TEFb with the G302-C324 apical region of the 3' hairpin of 7SK that is highly reminiscent of the human immunodeficiency virus transactivation-responsive RNA. PMID: 16382153
    38. Data show that the transcription-dependent dissociation of P-TEFb-HEXIM1-7SK RNA relies upon formation of hnRNP-7SK RNA complexes. PMID: 17709395
    39. provide structural insights how Hexim1 recognizes the Cyclin T1 subunit of positive transcription elongation factor b PMID: 17724342
    40. a mutant HEXIM1 protein that cannot be phosphorylated and released from P-TEFb and 7SK snRNA via the PI3K/Akt pathway antagonizes this HMBA-mediated induction of viral production. PMID: 17937499
    41. NPM binds to HEXIM1 in vitro and in vivo, and functions as a negative regulator of HEXIM1. PMID: 18371977
    42. HEXIM1 may act as a gene-selective transcriptional regulator via direct interaction with certain transcriptional regulators including glucocorticoid receptor. PMID: 18407829
    43. HEXIM1 regulation on estrogen action showed a decrease in estrogen-stimulated recruitment of ERalpha, P-TEFb, and S2P RNAP II to promoter and coding regions of ERalpha-responsive genes pS2 and CCND1 with increased HEXIM1 expression in MCF-7 cells PMID: 18757415
    44. HEXIM1 may participate in tissue-selective determination of glucocorticoid sensitivity via direct interaction with GR at least in certain gene sets including atp1a1 and scnn1a. PMID: 18801933
    45. Ubiquitination of HEXIM1 protein increases the inhibitory ability of HEXIM1 protein on P-TEFb-dependent transcription, but does not lead to proteasome-dependent protein degradation of HEXIM1. PMID: 19617712
    46. The tripartite protein-RNA complex formation between Hexim, Cyclin T and 7SK snRNA, was analyzed. PMID: 19883659
    47. Biochemically characterized a hexamethylene bisacetamide-inducible protein HEXIM1, which is a novel nuclear protein. Interacts with p50 of NF-kB and suppresses NF-kB transcription. PMID: 12581153

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Note=Binds alpha-importin and is mostly nuclear (PubMed:16362050).
  • 蛋白家族：
    HEXIM family
  • 组织特异性：
    Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues.
  • 数据库链接：

    HGNC: 24953

    OMIM: 607328

    KEGG: hsa:10614

    STRING: 9606.ENSP00000328773

    UniGene: Hs.586945