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  4. Recombinant Human Proteasome subunit beta type-5 (PSMB5)

Recombinant Human Proteasome subunit beta type-5 (PSMB5)

  • 货号：
    CSB-YP018883HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018883HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP018883HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018883HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018883HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PSMB5
  • Uniprot No.：
  • 别名：
    DKFZp459C139; EC 3.4.25.1; LMPX; Macropain epsilon chain; MB1; MGC104214; MGC118075; MGC134464; Multicatalytic endopeptidase complex epsilon chain; Proteasome (prosome, macropain) subunit, beta type, 5; Proteasome beta 5 subunit; Proteasome catalytic subunit 3; Proteasome chain 6; Proteasome epsilon chain; Proteasome subunit beta type-5; Proteasome subunit MB1; Proteasome subunit X; Proteasome subunit, beta type, 5; Proteasome subunit, beta-5; PSB5_HUMAN; PSMB5; PSX large multifunctional protease X; X
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    60-263
  • 氨基酸序列
    T TTLAFKFRHG VIVAADSRAT AGAYIASQTV KKVIEINPYL LGTMAGGAAD CSFWERLLAR QCRIYELRNK ERISVAAASK LLANMVYQYK GMGLSMGTMI CGWDKRGPGL YYVDSEGNRI SGATFSVGSG SVYAYGVMDR GYSYDLEVEQ AYDLARRAIY QATYRDAYSG GAVNLYHVRE DGWIRVSSDN VADLHEKYSG STP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Within the 20S core complex, PSMB5 displays a chymotrypsin-like activity.
  • 基因功能参考文献：
    1. Our results suggest a potential role for PSMB5 as a biomarker and therapeutic target for Triple-negative breast cancer PMID: 28623645
    2. Results identified PSMB5 Q62P mutation to underlie bortezomib resistance in Down-syndrome-associated acute myeloid leukemia cells. PMID: 28143565
    3. Data indicate that proteasomal subunit X PSMB5 is a target of signal transducer and activator of transcription 3 (STAT3). PMID: 24627483
    4. Nuclear MB1 expression was an independent predictor of worse survival in ovarian cancer. PMID: 19243813
    5. critical role of PSMB5 in 20S proteasome-mediated protection against replicative senescence, pointing to a possible strategy for maintaining the integrity of culture-expanded bone marrow stromal cells by manipulating the expression of PSMB5 PMID: 24393841
    6. The expression of proteasome beta5 decreases markedly in human atherosclerotic plaques. PMID: 22781773
    7. Data indicate that treatment-emergent resistance to single-agent bortezomib was independent of variants in the proteasome genes PSMB1, PSMB5, PSMB6, PSMB8, PSMB9, and PSMB10. PMID: 23018640
    8. Letter/Case Reports: proteasome subunit beta5t is expressed in cervical ectopic thymoma. PMID: 22523338
    9. PSMB5 overexpression is associated with Bortezomib resistance in myeloma. PMID: 21978467
    10. No mutations or differences in PSMB5 mRNA expression were seen before bortezomib treatment in 3 multiple myeloma patients, but after treatment, 1 patient showed PSMB5 upregulation associated with bortezomib resistance. PMID: 21920470
    11. CDK5 regulation of proteasome subunit PSMB5 was identified as a probable route to antineoplastic drug sensitization. PMID: 21289309
    12. Expression of mutated PSMB5 was associated with the prevention of the accumulation of unfolded proteins PMID: 20555361
    13. interaction of TAP1 and TAP2 and P-glycoprotein with proteasome subunits beta-5 and beta-5i suggest direct targeting of antigenic peptides to the ER via a TAP-proteasome association and a possible role for P-glycoprotein PMID: 15488952
    14. Proteasome activity can be genetically "upregulated" in lens cells by overexpression of beta5 catalytic subunit. Resulting increase in proteasome activity leads to decrease in oxidized proteins and enhanced cell survival following oxidative stress. PMID: 17262013
    15. G322A mutation of the PSMB5 gene is a novel mechanism for bortezomib resistance. PMID: 18502982
    16. nonsynonymous coding single nucleotide polymorphismsin the proteasome beta 5 subunit gene did not show significant effects on proteasome activity, but SNPs did influence transcription PMID: 18519783
    17. Overexpression of PSMB5 is an important mechanism for bortezomib resistance in Jurkat cell lines. PMID: 18562081
    18. a novel mechanism of bortezomib resistance associated with the selective overexpression of a mutant PSMB5 protein PMID: 18565852
    19. Mutations of the PSMB5 gene resulting in substitutions of Ala49 and Ala50 of PSMB5 protein can confer varying bortezomib resistance PMID: 19426847

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Peptidase T1B family
  • 数据库链接：

    HGNC: 9542

    OMIM: 600306

    KEGG: hsa:5693

    STRING: 9606.ENSP00000355325

    UniGene: Hs.422990