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  4. Recombinant Human Proliferation-associated protein 2G4 (PA2G4)

Recombinant Human Proliferation-associated protein 2G4 (PA2G4)

  • 货号：
    CSB-YP891987HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP891987HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP891987HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP891987HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP891987HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PA2G4
  • Uniprot No.：
  • 别名：
    38kDa; AA672939; Cell cycle protein p38 2G4 homolog; Cell cycle protein p38-2G4 homolog; ErbB-3 binding protein 1 ; ErbB3 binding protein 1 ; ErbB3-binding protein 1; ErbB3-binding protein Ebp1 ; hG4 1; hG4-1; IRES-specific cellular trans-acting factor 45 kDa; MGC81621; MGC94070; Mpp1; p38 2G4 ; Pa2g4; PA2G4_HUMAN; Plfap; Proliferation associated 2G4; Proliferation associated 2G4; 38-KD; Proliferation-associated 2G4; 38kDa ; Proliferation-associated 2G4; a; Proliferation-associated protein 1; Proliferation-associated protein 2G4; Protein p38-2G4; si:dz150i12.2; wu:fb19b11; wu:ft56d05; zgc:86732
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-394
  • 氨基酸序列
    SGEDEQQEQ TIAEDLVVTK YKMGGDIANR VLRSLVEASS SGVSVLSLCE KGDAMIMEET GKIFKKEKEM KKGIAFPTSI SVNNCVCHFS PLKSDQDYIL KEGDLVKIDL GVHVDGFIAN VAHTFVVDVA QGTQVTGRKA DVIKAAHLCA EAALRLVKPG NQNTQVTEAW NKVAHSFNCT PIEGMLSHQL KQHVIDGEKT IIQNPTDQQK KDHEKAEFEV HEVYAVDVLV SSGEGKAKDA GQRTTIYKRD PSKQYGLKMK TSRAFFSEVE RRFDAMPFTL RAFEDEKKAR MGVVECAKHE LLQPFNVLYE KEGEFVAQFK FTVLLMPNGP MRITSGPFEP DLYKSEMEVQ DAELKALLQS SASRKTQKKK KKKASKTAEN ATSGETLEEN EAGD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site). Regulates cell proliferation, differentiation, and survival. Isoform 1 suppresses apoptosis whereas isoform 2 promotes cell differentiation.
  • 基因功能参考文献：
    1. Targeted profiling of RNA translation reveals mTOR-4EBP1/2-independent translation regulation of mRNAs encoding ribosomal proteins. PMID: 30224479
    2. In this study, the s demonstrated that overexpression of ErbB3-binding protein 1 (EBP1) promoted not only a reduction of wild type of p85 subunit of phosphoinositide 3-kinase but also oncogenic mutant forms of p85 which were identified in human cancers. PMID: 27464702
    3. The results demonstrate an important role of Ebp1 in promoting cell proliferation in Acute Myelogenous Leukemic Cells through the regulation of both rRNA synthesis and Proliferating Cell Nuclear Antigen expression. PMID: 26813358
    4. results show that EBP1 interacts directly with PPIns and associate with PtdIns(3,4,5)P3 in the nucleolus. PMID: 27118868
    5. The adapter function of EBP1 P42 stabilized the interaction of FBXW7 with its substrates and promoted FBXW7-mediated degradation of oncogenic targets, enhancing its overall tumor-suppressing function. Results establish distinct physical and functional interactions between FBXW7 and EBP1 isoforms, which yield their mechanistically unique isoform-specific functions of EBP1 in cancer. PMID: 28209614
    6. The combined determination of Ebp1 and p53 expression levels in cervical cancer patients could support the effective prediction of metastatic potential and patient prognosis. PMID: 26436510
    7. Data highlight the tissue specificity function of EBP1 isoforms and show that only the oncogene p48 activates MHC II expression in human solid tumors, via STAT1 phosphorylation, in order to affect tumor progression by triggering specific immune response. PMID: 26081906
    8. Our results suggest a novel function of Ebp1 as a binding protein and negative regulator of Anxa2. The functional association between Anxa2 and EBP1 may participate in regulating cancer cell proliferation and invasion, contributing to cancer progression. PMID: 25917452
    9. Ebp1 functionality is independent from heat-shock-protein-regulated progression networks in prostate cancer. PMID: 24798454
    10. P48Ebp1 acts as an oncoprotein. PMID: 25154617
    11. EBP1 participates in the regulation of intestinal inflammation via mediating Akt signaling pathway. PMID: 26256794
    12. Data suggest that inhibiting ErbB3-binding protein 1 Ebp1 phosphorylation may be an effective mechanism for inhibiting T-cell activation and proliferation. PMID: 25691158
    13. Ebp1 p42 isoform regulates the proteasomal degradation of the p85 regulatory subunit of PI3K by recruiting a chaperone-E3 ligase complex HSP70/CHIP. PMID: 24651434
    14. data suggest that Vpr may inhibit Ebp1 to stabilize p53, which in turn leads to G2 arrest and apoptosis in U87MG cells PMID: 23828502
    15. identified among endothelial antigens to which antibodies are produced during heart transplant rejection PMID: 23707440
    16. The major effect of EBP1 on ErbB2 mRNA expression levels is at the transcriptional level. PMID: 23242156
    17. Down-regulation of the ErbB3 binding protein 1 in human bladder cancer promotes tumor progression and cell proliferation. PMID: 23283744
    18. Performed immunohistochemical analysis on 132 primary adenoid cystic carcinoma and adjacent non-cancerous tissues.The expression of EBP1 was significantly higher in non-cancerous adjacent tissues compared with corresponding cancer tissues. PMID: 23110497
    19. It was shown that high level of Ebp1 expression led to enhancee HDM2 phosphorylation by Akt and inhibited the self-ubiquitination of HDM2 by up-regulation of Akt activity. PMID: 21930127
    20. the Ebp1 promoter localizes between -664 nt and the initiation site of the Ebp1 gene, +317-nt long sequence in the noncoding region is required for regulation of Ebp1 gene expression. PMID: 21794029
    21. These studies suggest that one pathway of EBP1 down-regulation of AR levels may be lost in castration-resistant prostate cancer. PMID: 21965718
    22. Ebp1 contributes to neuronal cell differentiation and growth factor specificity through the activation of protein kinase Cdelta acting as a crucial downstream effector of neurotrophin signaling. PMID: 21145366
    23. Ablation of EBP1 expression led to tamoxifen resistance in breast cancer. PMID: 20379846
    24. Data show that E2F is likely a central coordinator of multiple responses that culminate in regulation of EBP1 gene expression, and which may vary depending on cell type and context. PMID: 21085677
    25. Findings suggest that eBP1 p48 functions as an oncogene by promoting glioma tumorigenicity via interactions with HDM2 that contribute to p53 downregulation. PMID: 21098709
    26. our results reveal an EBP1-Foxa-AGR2 signaling circuit with functional significance in metastatic prostate cancer PMID: 20048076
    27. EBP1 is in the cytoplasm & nucleolus. Nucleolar localization requires AA sequences at the NH2- and COOH-ends. Overexpression inhibits proliferation of human fibroblasts. It is part of RNP complexes & associates with different rRNAs. PMID: 15064750
    28. ErbB3-binding protein Ebp1 binding to E2F promoter elements and E2F-mediated transcription are regulated by heregulin. PMID: 15073182
    29. These studies suggest that Ebp1 is an AR corepressor whose biological activity can be regulated by the ErbB3 ligand, HRG. PMID: 15583694
    30. Ebp1 suppresses androgen receptor-mediated gene transcription and tumorigenesis of prostate cancer cells. PMID: 15994225
    31. Ebp1 is present in protein-ARE(bcl-2) RNA complexes. Ebp1 & nucleolin are present in the same bcl-2 mRNP complexes. Ebp1 decreases the rate of decay of beta-globin-ARE(bcl-2) transcripts. Ebp1 contributes to bcl-2 expression regulation in HL-60 cells. PMID: 16396631
    32. Our results demonstrate that Ebp1 is a new dsRNA-binding protein that acts as a cellular inhibitor of eIF2alpha phosphorylation suggesting that it could be involved in protein translation control PMID: 16631606
    33. over-expression of Ebp1 interfered with virus production PMID: 17295834
    34. PKC-delta antagonizes apoptosis through phosphorylating Ebp1 and protects it from apoptotic degradation. PMID: 17316401
    35. ErbB-3-binding protein-1 is an immunogenic protein with cancer-related immunoreactivity, capable of eliciting CD8-positive T cell-mediated responses in vivo and in vitro. PMID: 17641068
    36. The structure provides insights in how Ebp1 discriminates between its different interaction partners. PMID: 17765895
    37. ErbB-3-binding protein 1 (Ebp1) is a member of the family of proliferation-associated 2G4 proteins (PA2G4s) and plays a role in cellular growth and differentiation. PMID: 17768350
    38. These results suggest that EBP1, by down-regulating ErbB signal transduction, attentuates HRG-mediated growth of breast cancer cells. PMID: 18355957
    39. These data support a role for EBP1 in the development of hormone-refractory prostate cancer. PMID: 18852121
    40. hBre1 inhibits Ebp1's tumor suppressive activity through mediating its polyubiquitination and degradation. PMID: 19037095

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  • 亚细胞定位：
    [Isoform 1]: Cytoplasm. Nucleus, nucleolus.; [Isoform 2]: Cytoplasm.
  • 蛋白家族：
    Peptidase M24 family
  • 组织特异性：
    Isoform 2 is undetectable whereas isoform 1 is strongly expressed in cancer cells (at protein level). Isoform 1 and isoform 2 are widely expressed, including heart, brain, lung, pancreas, skeletal muscle, kidney, placenta and liver.
  • 数据库链接：

    HGNC: 8550

    OMIM: 602145

    KEGG: hsa:5036

    STRING: 9606.ENSP00000302886

    UniGene: Hs.524498