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  4. Recombinant Human Profilin-1 (PFN1)

Recombinant Human Profilin-1 (PFN1)

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  • 货号：
    CSB-EP017824HU
  • 规格：
    ￥1344
  • 促销：
    促销单品低至599元起
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Actin binding protein ; ALS18; Epididymis tissue protein Li 184a; OTTHUMP00000125244; PFN 1; Pfn; PFN1; PROF1_HUMAN; Profilin I; Profilin-1; Profilin1; ProfilinI
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    41.9kDa
  • 表达区域：
    2-140aa
  • 氨基酸序列
    AGWNAYIDNLMADGTCQDAAIVGYKDSPSVWAAVPGKTFVNITPAEVGVLVGKDRSSFYVNGLTLGGQKCSVIRDSLLQDGEFSMDLRTKSTGGAPTFNVTVTKTDKTLVLLMGKEGVHGGLINKKCYEMASHLRRSQY
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.
  • 基因功能参考文献：
    1. Variants of rs238243 and rs238238 might regulate profilin1 expression by epigenetic modification and indirectly affects the susceptible threshold of hypertension in Chinese Han population. PMID: 28541412
    2. Results suggested that the RhoA/ROCK1 pathway activated by excessive ROS is responsible for profilin-1-mediated endothelial damage. PMID: 29849894
    3. PFN1 could promote autophagy through taking part in Beclin1 complex and contribute to bortezomib resistance, which may become a novel molecular target in the therapy of MM. PMID: 29945297
    4. Loss of PFN1 in tumor cells has been associated with lymph node invasion and metastasis in other tumor types, strengthening the argument that the protein has the potential to be a tumor suppressor in late-stage oral squamous cell carcinoma. PMID: 27862305
    5. Guttiferone K effectively suppresses the motility and metastasis of hepatocellular carcinoma cells mainly by restoration of aberrantly reduced PFN1 protein expression PMID: 27494863
    6. Results collectively suggest that PFN1 promotes cell migration and adhesion in bladder cancer models. PMID: 27683119
    7. These results suggest that although mutant PFN1 aggregation may contribute to neurodegeneration, it does not trigger its onset. Importantly, these experiments establish a progressive disease model that can contribute toward identifying the mechanisms of ALS pathogenesis and the development of therapeutic treatments. PMID: 27681617
    8. One potential mechanism for C71G-PFN1 to initiate Amyotrophic lateral sclerosis might be the abnormal interaction with membranes as recently established for SOD1 mutants. PMID: 28847504
    9. Expression of PFN1 mutants induces accumulation of TDP-43, and promotes conversion of normal TDP-43 into an abnormal form. These results provide new insight into the mechanisms of TDP-43 proteinopathies and other diseases associated with amyloid-like protein deposition. PMID: 27432186
    10. We suggest that reduction of PFN-1 expression by elevated levels of PrP(c) may contribute to protective effects PrP(c)-overexpressing SH-SY5Y cells confer against STS-induced apoptosis PMID: 28102851
    11. this study shows that in pancreatic cancer patients, PFN1 expression is substantially decreased in peripheral CD8(+) T cells PMID: 28688208
    12. mutant profilin1 in various diseases with an emphasis on its contribution to the pathogenesis of amyotrophic lateral sclerosis (Review) PMID: 27669692
    13. Data suggest 2 major isoforms of profilin (Pfn1 and Pfn2) are co-regulated by a common mechanism involving the action of MKL1 [megakaryoblastic leukemia (translocation) 1 protein] that is independent of its SRF- (serum-response factor)-related activity; cellular externalization of Pfn1, rather than transcription, is affected by the perturbations of MKL1; MKL1 can influence cell migration by modulating Pfn1 expression. PMID: 28546428
    14. novel profilin-1 variants associated with amyotrophic lateral sclerosis PMID: 27101547
    15. We found that ARP3 and profilin1 were 2 binding partners of LMO2, primarily in cytoplasm. LMO2. LMO2 mediated the assembly of a complex including ARP3, profilin1, and actin monomer, increased actin monomer binding to profilin1, and promoted lamellipodia/filopodia formation in basal-type breast cancer cells. PMID: 28170369
    16. These observations indicate that our novel profilin1 mutant mouse line may provide a new ALS model with the opportunity to gain unique perspectives into mechanisms of neurodegeneration that contribute to ALS pathogenesis. PMID: 28040732
    17. These data suggest that Familial Amyotrophic Lateral Sclerosis-linked PFN1 mutations exacerbate TDP-43-induced neurodegeneration in a gain-of-function manner, possibly by shifting the localization of TDP-43 from nuclei to cytoplasm. PMID: 27634045
    18. Homo-oligomerization of the actin-binding protein PFN1 has been characterized by the relaxation dispersion profiles of the protein as a function of concentration. PMID: 28052669
    19. Gain-of-toxic-function PFN1 gene mutation leads to conformational change of TDP-43 and to neurodegeneration in amyotrophic lateral sclerosis. PMID: 26908597
    20. Profilin synergizes with chemotherapeutic drugs to induce tumor cell death by regulating NF-kappaB and p53. Thus, modulation of Profilin may be a useful strategy for effective combination therapy. PMID: 26842845
    21. Mutations of profilin-1, associated with familial amyotrophic lateral sclerosis, increase the tendency of profilin-1 to aggregate and that such aggregation behavior is largely determined by the mutation-induced structural changes occurring in the folded state of the protein. PMID: 26226631
    22. evidence, which suggests that Profilin increases tumour suppressor activity by regulating NF-kappaB. PMID: 26787927
    23. Profilin-1 folding process occurs in the absence of thermodynamically stable partially folded states. PMID: 26227615
    24. Actin independent mechanisms contribute to the pathogenicity of PFN1 T109M and possibly other PFN1 mutations. PMID: 26572741
    25. expression of the ALS-associated actin-binding deficient mutant of PFN1 (PFN1(C71G)) results in increased dendritic arborisation and spine formation, and cytoplasmic inclusions in cultured mouse hippocampal neurons PMID: 26499959
    26. PFN1 is a rare cause of ALS. PMID: 25499087
    27. findings suggest that a destabilized form of PFN1 underlies PFN1-mediated ALS pathogenesis PMID: 26056300
    28. Suggest that PFN1 plays a critical role in gastric carcinoma progression, and these effects are likely mediated through the integrin beta1/FAK pathway. PMID: 25741138
    29. Data indicated that No PFN1 mutations were identified in the Catalan population with amyotrophic lateral sclerosis. PMID: 25249294
    30. Profilin-1 overexpression in MDA-MB-231 breast cancer cells is associated with alterations in proteomics biomarkers of cell proliferation, survival, and motility as revealed by global proteomics analyses PMID: 25454514
    31. Profilin1 acts as a molecular regulator of the levels of PI(3,4)P2 and Tks5 recruitment in invadopodia to control the invasion efficiency of invadopodia. PMID: 25613364
    32. Collective expression pattern of tensin/profilin-1/villin-1/talin could be a biomarker to estimate the prognosis of esophageal squamous cell carcinoma patients. PMID: 25337239
    33. Pfn1 is a tumor suppressor in pancreatic cancer that acts via a novel mechanism of regulating the SIRT3-HIF1alpha axis. PMID: 25103363
    34. Higher messenger RNA expression of Profilin-1 is associated with significantly lower survival PMID: 25704627
    35. the exchange of bound actin between Tbeta4 and profilin involves both steric and allosteric components. PMID: 25313062
    36. effects of profilin-1 and profilin-2, the two major isoforms of profilin, on actin cytoskeletal regulation, motility, and invasion of breast cancer cells PMID: 23827010
    37. This review summarize the PFN1 most recently discovered 'high risk' genes in ALS. PMID: 24780888
    38. association of cortactin with Pfn-1 is regulated by c-Abl-mediated cortactin phosphorylation PMID: 24700464
    39. Raising the intracellular levels of Profilin I decreased the mobile fraction ratio of actin filaments and slowed their polymerization rate. PMID: 24465723
    40. In glioblastomas endothelial cell-specific Pfn-1 phosphorylation elevates HIF-1alpha expression leading to vascular abnormalities and tumor progression. PMID: 24747440
    41. The ALS-linked mutations in profilin 1 alter stress granule dynamics, providing further evidence for the potential role of stress granules in ALS pathogenesis. PMID: 24920614
    42. CHIP regulates Pfn1 levels as an E3 ligase, and possibly plays a role in cell migration and metastasis of breast cancer. PMID: 24661873
    43. Data indicate that lower profilin1 (Pfn1) expression is associated with increased metastatic potential in breast cancer. PMID: 23686314
    44. PFN1 mutations were identified in autosomal dominant FALS patients. PMID: 24085347
    45. Profilin-1 might act as an ultimate and common cellular effector in the process of metabolic memory (endothelial abnormalities) mediated by AGEs via the ROS/PKC or ROS/NF-B signalling pathways. PMID: 24090212
    46. Wanted to identify estrogen receptor alpha (ERalpha) interacting proteins in Tamoxifen treated MCF7 cells. Using a GST-pull down assay with ERalpha ligand-binding domain and MS-based proteomics approach we identified Profilin1 as a novel ERalpha interacting protein. PMID: 23576398
    47. up-regulation of profilin1 facilitated apoptosis and repressed autophagy induced by irradiation. PMID: 23826918
    48. PFN1 mutations lead to ubiquitin-positive inclusions and impairment of cytoskeletal pathways, in which, a pathophysioloy of familial and sporadic ALS lays. PMID: 23635659
    49. The single nucleotide polymorphism (SNP) rs13204 of the PFN1 gene has an important function in the development of amyotrophic lateral sclerosis in Han Chinese. PMID: 23428184
    50. PFN1 mutations are not a common cause of frontotemporal lobar degeneration and amyotrophic lateral sclerosis in this cohort of patients from France. PMID: 23182804

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  • 相关疾病：
    Amyotrophic lateral sclerosis 18 (ALS18)
  • 亚细胞定位：
    Cytoplasm, cytoskeleton.
  • 蛋白家族：
    Profilin family
  • 组织特异性：
    Expressed in epididymis (at protein level).
  • 数据库链接：

    HGNC: 8881

    OMIM: 176610

    KEGG: hsa:5216

    STRING: 9606.ENSP00000225655

    UniGene: Hs.494691