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  4. Recombinant Human Probable E3 ubiquitin-protein ligase TRIM8 (TRIM8)

Recombinant Human Probable E3 ubiquitin-protein ligase TRIM8 (TRIM8)

  • 货号：
    CSB-YP871629HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP871629HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP871629HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP871629HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP871629HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TRIM8
  • Uniprot No.：
  • 别名：
    AA408830; BB006755; GERP; Glioblastoma-expressed RING finger protein; Lioblastoma expressed ring finger protein; Probable E3 ubiquitin-protein ligase TRIM8; RING finger protein 27; RNF27; RP11-47A8.4; TRIM8; TRIM8_HUMAN; Tripartite motif containing 8; Tripartite motif protein TRIM8; Tripartite motif-containing protein 8
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-551
  • 氨基酸序列
    MAENWKNCFE EELICPICLH VFVEPVQLPC KHNFCRGCIG EAWAKDSGLV RCPECNQAYN QKPGLEKNLK LTNIVEKFNA LHVEKPPAAL HCVFCRRGPP LPAQKVCLRC EAPCCQSHVQ THLQQPSTAR GHLLVEADDV RAWSCPQHNA YRLYHCEAEQ VAVCQYCCYY SGAHQGHSVC DVEIRRNEIR KMLMKQQDRL EEREQDIEDQ LYKLESDKRL VEEKVNQLKE EVRLQYEKLH QLLDEDLRQT VEVLDKAQAK FCSENAAQAL HLGERMQEAK KLLGSLQLLF DKTEDVSFMK NTKSVKILMD RTQTCTSSSL SPTKIGHLNS KLFLNEVAKK EKQLRKMLEG PFSTPVPFLQ SVPLYPCGVS SSGAEKRKHS TAFPEASFLE TSSGPVGGQY GAAGTASGEG QSGQPLGPCS STQHLVALPG GAQPVHSSPV FPPSQYPNGS AAQQPMLPQY GGRKILVCSV DNCYCSSVAN HGGHQPYPRS GHFPWTVPSQ EYSHPLPPTP SVPQSLPSLA VRDWLDASQQ PGHQDFYRVY GQPSTKHYVT S
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase that participates in multiple biological processes including cell survival, differentiation, apoptosis, and in particular, the innate immune response. Participates in the activation of interferon-gamma signaling by promoting proteasomal degradation of the repressor SOCS1. Plays a positive role in the TNFalpha and IL-1beta signaling pathways. Mechanistically, induces the 'Lys-63'-linked polyubiquitination of MAP3K7/TAK1 component leading to the activation of NF-kappa-B. Modulates also STAT3 activity through negative regulation of PIAS3, either by degradation of PIAS3 through the ubiquitin-proteasome pathway or exclusion of PIAS3 from the nucleus. Negatively regulates TLR3/4-mediated innate immune response by catalyzing 'Lys-6'- and 'Lys-33'-linked polyubiquitination of TICAM1 and thereby disrupting the TICAM1-TBK1 interaction.
  • 基因功能参考文献：
    1. TRIM8 activates STAT3 by suppressing the expression of PIAS3, an inhibitor of STAT3, most likely through E3-mediated ubiquitination and proteasomal degradation. PMID: 28100038
    2. we provided evidence that TRIM8 and its regulators miR-17-5p and miR-106b-5 participate to a feedback loop controlling cell proliferation through the reciprocal modulation of p53, miR-34a and N-MYC. PMID: 28327152
    3. A pathogenic effect of a heterozygous truncating mutation in the tripartite motif containing 8 (TRIM8) gene on the postnatal development of the human brain. PMID: 27346735
    4. Our study provides evidences that TRIM8 may participate in the carcinogenesis and progression of glioma and that the transcriptional repression of TRIM8 might have potential value for predicting poor prognosis in glioma patients. PMID: 26077989
    5. These findings provide the first mechanistic link between TRIM8 and the drug resistance of clear cell Renal Cell Carcinoma PMID: 25277184
    6. Nucleo-cytoplasmic trafficking of TRIM8, a novel oncogene, is involved in positive regulation of TNF induced NF-kappaB pathway. PMID: 23152791
    7. TRIM8 is a p53 direct target gene that induces p53 stabilization and promotes the degradation of MDM2 protein. PMID: 22262183
    8. Tripartite motif 8 (TRIM8) modulates TNFalpha- and IL-1beta-triggered NF-kappaB activation by targeting TAK1 for K63-linked polyubiquitination. PMID: 22084099
    9. TRIM8 modulates translocation of phosphorylated STAT3 into the nucleus through interaction with Hsp90beta and consequently regulates transcription of Nanog in embryonic stem cells. PMID: 21689689
    10. These findings indicate that TRIM8 enhances the STAT3-dependent signal pathway by inhibiting the function of PIAS3. PMID: 20516148
    11. interacts with SOCS-1 PMID: 12163497
    12. Gerp transcribes interferon-gamma in epithelial and lymphoid cells and is expressed almost ubiquitously in tissues. PMID: 15345195
    13. Among genes correlated to nodal metastatic progression, we verified in vitro that NM23-H3 reduced cell motility and TRIM8 were a growth suppressor. PMID: 17900511

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    TRIM/RBCC family
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 15579

    OMIM: 606125

    KEGG: hsa:81603

    STRING: 9606.ENSP00000302120

    UniGene: Hs.336810