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  4. Recombinant Human Probable ATP-dependent RNA helicase DHX58 (DHX58)

Recombinant Human Probable ATP-dependent RNA helicase DHX58 (DHX58)

  • 中文名称：
    人DHX58重组蛋白
  • 货号：
    CSB-YP822187HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人DHX58重组蛋白
  • 货号：
    CSB-EP822187HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人DHX58重组蛋白
  • 货号：
    CSB-EP822187HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人DHX58重组蛋白
  • 货号：
    CSB-BP822187HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人DHX58重组蛋白
  • 货号：
    CSB-MP822187HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    D11LGP2; D11lgp2e; DEXH (Asp Glu X His) box polypeptide 58; DEXH box polypeptide 58; DHX 58; DHX58; DHX58_HUMAN; LGP 2; LGP2; Ortholog of mouse D11lgp2; Probable ATP dependent helicase LGP2; Probable ATP dependent RNA helicase DHX58; Probable ATP-dependent helicase LGP2; Probable ATP-dependent RNA helicase DHX58; Protein D11Lgp2 homolog; RIG-I-like receptor LGP2; RLR; RNA helicase LGP2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-678
  • 氨基酸序列
    MELRSYQWEV IMPALEGKNI IIWLPTGAGK TRAAAYVAKR HLETVDGAKV VVLVNRVHLV TQHGEEFRRM LDGRWTVTTL SGDMGPRAGF GHLARCHDLL ICTAELLQMA LTSPEEEEHV ELTVFSLIVV DECHHTHKDT VYNVIMSQYL ELKLQRAQPL PQVLGLTASP GTGGASKLDG AINHVLQLCA NLDTWCIMSP QNCCPQLQEH SQQPCKQYNL CHRRSQDPFG DLLKKLMDQI HDHLEMPELS RKFGTQMYEQ QVVKLSEAAA LAGLQEQRVY ALHLRRYNDA LLIHDTVRAV DALAALQDFY HREHVTKTQI LCAERRLLAL FDDRKNELAH LATHGPENPK LEMLEKILQR QFSSSNSPRG IIFTRTRQSA HSLLLWLQQQ QGLQTVDIRA QLLIGAGNSS QSTHMTQRDQ QEVIQKFQDG TLNLLVATSV AEEGLDIPHC NVVVRYGLLT NEISMVQARG RARADQSVYA FVATEGSREL KRELINEALE TLMEQAVAAV QKMDQAEYQA KIRDLQQAAL TKRAAQAAQR ENQRQQFPVE HVQLLCINCM VAVGHGSDLR KVEGTHHVNV NPNFSNYYNV SRDPVVINKV FKDWKPGGVI SCRNCGEVWG LQMIYKSVKL PVLKVRSMLL ETPQGRIQAK KWSRVPFSVP DFDFLQHCAE NLSDLSLD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1-dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses and coronavirus SARS-CoV-2, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate.
  • 基因功能参考文献：
    1. Study demonstrated a tumor suppressor role of LGP2 in neuroblastoma (NB). LGP2 was effective in promoting poly (I:C)-induced NB suppression and cytoplasmic LGP2 can serve as an independent favorable prognostic factor in NB patients. PMID: 30179292
    2. genetic loss of LGP2 uncovers dsRNA-mediated RNAi albeit less strongly than complete loss of the IFN system PMID: 29351913
    3. Results indicate that pumilio RNA binding family member 1 (PUM1) is a negative regulator of RNA helicase LGP2 (LGP2), a master regulator of innate immunity genes expressed in a cascade fashion. PMID: 28760986
    4. study documents that recombinant measles virus produce defective interfering genomes that have high immunostimulatory properties via their binding to RIG-I and LGP2 proteins, both of which are cytosolic nonself RNA sensors of innate immunity. PMID: 28768856
    5. essential role in activating interferon signaling against hepatitis C virus (HCV) infection by promoting MDA5 recognition of HCV pathogen-associated molecular patterns PMID: 28090671
    6. LGP2 did not reveal significant single-SNP associations with multiple sclerosis risk. PMID: 25288302
    7. LGP2 increases the initial rate of MDA5-RNA interaction and regulates MDA5 filament assembly. PMID: 25127512
    8. results demonstrate previously unrecognized properties of LGP2 ATP hydrolysis and RNA interaction and provide a mechanistic basis for a positive regulatory role for LGP2 in antiviral signaling PMID: 23184951
    9. LGP2, a host protein induced during influenza A virus infection, downregulates the host antiviral IFN response PMID: 22837208
    10. Paramyxovirus 5 V proteins interact with the RNA Helicase LGP2 to inhibit RIG-I-dependent interferon beta induction. PMID: 22301134
    11. LGP2 functions as an inhibitor of the innate immune response triggered by Rabeis virus infection PMID: 21525357
    12. LGP2 competes with the kinase IKKi for a common interaction site on IPS-1, as an element of negative-feedback regulation of intracellular antiviral signaling. PMID: 17020950
    13. LGP2 can be coexpressed with RIG-I to inhibit activation of the NF-kappaB reporter expression and that LGP2 protein produced in insect cells can bind both single- and double-stranded RNA (dsRNA), with higher affinity and cooperativity for dsRNA. PMID: 18411269
    14. The RIG-I-like receptor LGP2 recognizes the termini of double-stranded RNA. PMID: 19278996
    15. Cloning and expression of the mouse Lpg2 mRNA, and comparison of the ORF to that of human LPG2 mRNA. PMID: 11735219

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Helicase family, RLR subfamily
  • 组织特异性：
    Expressed in testis, nerve and spleen. Also expressed in the brain.
  • 数据库链接：

    HGNC: 29517

    OMIM: 608588

    KEGG: hsa:79132

    STRING: 9606.ENSP00000251642

    UniGene: Hs.55918