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  4. Recombinant Human Polyadenylate-binding protein 1 (PABPC1), partial

Recombinant Human Polyadenylate-binding protein 1 (PABPC1), partial

  • 货号：
    CSB-YP017352HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP017352HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP017352HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP017352HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    PABPC1; PAB1; PABP1; PABPC2; Polyadenylate-binding protein 1; PABP-1; Poly(A)-binding protein 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 表达区域：
    1-370
  • 氨基酸序列
    MNPSAPSYPMASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYGFVHFETQEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAELGARAKEFTNVYIKNFGEDMDDERLKDLFGKFGPALSVKVMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRKFEQMKQDRITRYQGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKVMMEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds the poly(A) tail of mRNA, including that of its own transcript, and regulates processes of mRNA metabolism such as pre-mRNA splicing and mRNA stability. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. By binding to long poly(A) tails, may protect them from uridylation by ZCCHC6/ZCCHC11 and hence contribute to mRNA stability.; (Microbial infection) Positively regulates the replication of dengue virus (DENV).
  • 基因功能参考文献：
    1. Study characterized the structure of multiple PABPC1 molecules bound to a long poly(A) chain as constituting a one-dimensional wormlike form. These interactions responsible for PABPC1 multimerization occur at residues in the linker region and the RNA recognition motif 2, and shown that such interaction is important for translational activity. PMID: 29362417
    2. Epstein-Barr Virus protein EB2 first is recruited to the mRNA cap structure in the nucleus and then interacts with the proteins eIF4G and PABP to enhance the initiation step of translation. PMID: 29142127
    3. These findings identify PABPC1 as a direct regulator of cardiac hypertrophy and define a new paradigm of gene regulation in the heart, where controlled changes in poly(A) tail length influence mRNA translation. PMID: 28653618
    4. Data suggest that DEAD-box helicase 3 (DDX3X) physically interacts and co-localizes with poly(A)-binding cytoplasmic protein 1 (PABPC1) and caprin-1 in lamellipodia at the leading edge of spreading cells; these interactions are dependent on mRNA; depletion of DDX3X (via gene silencing with the CRISPR-Cas system) leads to decreased cell motility. These studies were conducted using MRC5 lung fibroblast cell line. PMID: 28733330
    5. Data suggest that hnRNPLL specifically associates with cytoplasmic PABPC1 in both T-lymphocytes and plasma cells; PABPC1 promotes binding of hnRNPLL to immunoglobulin H (IgH, heavy chain) mRNA and regulates switching from mIgH (membrane isoform) to sIgH (secreted isoform) in plasma cells. (hnRNPLL = heterogeneous nuclear ribonucleoprotein L-like protein; PABPC1 = cytoplasmic poly[A]-binding protein 1) PMID: 28611064
    6. PABP enhances the productive binding of the eRF1-eRF3 complex to the ribosome, via interactions with the N-terminal domain of eRF3a which itself has an active role in translation termination. PMID: 27418677
    7. Unr stimulated the binding of PABP1 to mRNA, and that Unr was required for the stable interaction of PABP1 and eIF4G in cells. PMID: 26936655
    8. PABPC1 decreased expression in in infertile men with non-obstructive azoospermia PMID: 26843391
    9. BTG2 stimulates mRNA deadenylation via CAF1 activation through interaction with PABPC1. Interaction of BTG2 with the first RRM domain of PABPC1 is required for BTG2 to control cell proliferation. PMID: 26912148
    10. PABPC1 is a novel co-regulator of the AR PMID: 26176602
    11. PABPC1 is upregulated in gastric carcinoma tissues, and high PABPC1 expression predicts poor survival. PABPC1 regulates proliferation and transformation of gastric cancer cells in vitro and in vivo. PABPC1 knockdown induces apoptosis. PMID: 26097561
    12. PABPC1 interacts with AGO2 and is responsible for the microRNA mediated gene silencing in high grade hepatocellular carcinoma. PMID: 26188282
    13. Poly(A) binding protein 1 enhances cap-independent translation initiation of neurovirulence factor from avian herpesvirus PMID: 25503397
    14. Metastatic duodenal cancer cells do not tolerate intracellular PABP1 accumulation and are thus exported into the extracellular milieu by the exosome-mediated pathway. In addition, PABP1 has a potential use as a biomarker for metastatic duodenal cancer. PMID: 25065644
    15. This study highlights PABP-1 as a protein that is important to the pathology of ALS and indicates that the proteomic profile of TDP-43 inclusions in ALS may differ depending on the causative genetic mutation. PMID: 25111021
    16. PABPC1 is mislocalized in spinal cord motor neurons in amyotrophic lateral sclerosis. PMID: 24336168
    17. Study shows that PABP specifically regulates iNOS mRNA stability in human DLD-1 cells by binding to different sites in the 50- and 30-UTR of the iNOS mRNA. . PMID: 23711718
    18. These results suggest that production of the NSs protein during rift valley fever virus infection leads to sequestration of PABP1 in the nuclear speckles, creating a state within the cell that favors viral protein production. PMID: 23966414
    19. These data indicate that PABPC1 is an important cellular target of human herpesvirus 8 ORF57 to directly upregulate polyadenylated nuclear RNA accumulation during viral lytic infection. PMID: 23077296
    20. Data show that eIF4G interacts with the RRM2 domain of polyadenylate-binding protein-1 (PABP). PMID: 23041282
    21. data suggest that PABPC1 is essential for the formation of L1 RNA-protein complexes and may play a role in L1 RNP translocation in the host cell PMID: 22907758
    22. EMCV 3C proteinase mediates site-specific PABP cleavage and demonstrate that PABP cleavage by 3C regulates EMCV replication PMID: 22837200
    23. This finding demonstrates that viruses can increase host translation initiation factor concentration to foster their replication and defines a unique mechanism whereby control of PABP abundance regulates eIF4F assembly. PMID: 22431630
    24. a role for PABPC1 and associated translation initiation factors in nonsense-mediated mRNA decay (NMD) evasion of AUG-proximal nonsense-mutated transcripts PMID: 21989405
    25. study presents evidence that the function of KSHV polyadenylated nuclear (PAN) RNA is to bind poly(A) binding protein, which normally binds poly(A) tails of mRNAs in the cytoplasm but is re-localized into the nucleus during lytic KSHV infection PMID: 22022268
    26. A direct link between PABP1 modification status and the formation of distinct mRNP (messenger ribonucleoprotein) complexes that regulate mRNA fate in the cytoplasm. PMID: 22004688
    27. The present study has characterized DDX3 as a pivotal SG-nucleating factor and illustrates co-ordinative roles for DDX3, eIF4E and PABP1 in integrating environmental stress with translational regulation. PMID: 21883093
    28. Nuclear relocalisation of cytoplasmic poly(A)-binding proteins PABP1 and PABP4 in response to UV irradiation reveals mRNA-dependent export PMID: 21940797
    29. in the absence of PABP, the glycolytic enzyme GAPDH translocated to the cell nucleus and activated the GAPDH mediated apoptotic pathway by enhancing acetylation and serine 46 phosphorylation of p53. PMID: 21539808
    30. these results suggest that depletion of PABP prevents protein synthesis and consequently leads to cell death through apoptosis. PMID: 21521633
    31. LARP4 activity is integrated with other PAM2 protein activities by PABP as part of mRNA homeostasis. PMID: 21098120
    32. Cytoplasmic PABP accumulation is translationally controlled in human cytomegalovirus-infected cells. PMID: 20980505
    33. These findings reveal that despite species-specific differences in the relative strength of the PABPC1-binding sites, the interaction between GW182 proteins and PABPC1 is critical for miRNA-mediated silencing in animal cells. PMID: 21063388
    34. Data show that in addition to PP2Ac, alpha4 interacts with EDD and PABP, suggesting its involvement in multiple steps in the mTOR pathway that leads to translation initiation and cell-cycle progression. PMID: 20544796
    35. The cytoplasmic poly (A)-binding protein (PABP) plays a crucial role in regulating both translation and stability of eukaryotic mRNA. PMID: 20009508
    36. PABP relocalization in infection was found to be independent of the viral protein ICP27. PMID: 20573819
    37. Structural basis of binding of P-body-associated proteins GW182 and ataxin-2 by the Mlle domain of poly(A)-binding protein.( PMID: 20181956
    38. HIV- 1 protease inhibits Cap- and poly(A)-dependent translation upon eIF4GI and PABP cleavage PMID: 19956697
    39. The side chain of the invariant MLLE residue K580 of poly(A)-binding protein forms hydrogen bonds with the backbone of PAM2 residues 5 and 7. PMID: 20096703
    40. The s show that a conserved motif in the human GW182 paralog TNRC6C interacts with the C-terminal domain of polyadenylate binding protein 1 (PABC) and present the crystal structure of the complex. PMID: 20098421
    41. Bunyamwera virus triggers nuclear retention of PABP in mammalian cells. PMID: 19193790
    42. Poly(A)-binding protein modulates mRNA susceptibility to cap-dependent miRNA-mediated repression. PMID: 19934229
    43. Methylated in vivo by CARM1. Methylated region is mapped. PMID: 11850402
    44. PABP1 is a potential substrate in MAPKAP kinase 2-induced mRNA stabilization. PMID: 12565831
    45. targeted degradation of PABP contributes to translation inhibition in apoptotic cells PMID: 14739600
    46. PABP1 associates with paxillin in order to be efficiently transported from the nucleus to the cytoplasm; this event is necessary for cells to remodel their focal adhesions during cell migration. PMID: 15831480
    47. a novel signaling pathway involving MKK-2 and ERK1/2 may down-regulate the activity of PABP and eIF4E by controlling their phosphorylation and compensates for the effect of excess cellular PABP PMID: 16332685
    48. In esophageal cancer, reduced expression of PABPC1 was correlated with local tumor progression and poor prognosis after surgery. PMID: 16465428
    49. PABP interacts with HHV-8 K10/10.1 protein in infected primary effusion lymphoma (PEL) cell lines. PMID: 16716377
    50. poly(A)-binding protein 1 (PABP) as a novel BRCA1-interacting protein. PMID: 16782705

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, Stress granule. Nucleus. Cell projection, lamellipodium.
  • 蛋白家族：
    Polyadenylate-binding protein type-1 family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    HGNC: 8554

    OMIM: 604679

    KEGG: hsa:26986

    STRING: 9606.ENSP00000313007

    UniGene: Hs.387804