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  4. Recombinant Human Phosphatidylinositol-binding clathrin assembly protein (PICALM)

Recombinant Human Phosphatidylinositol-binding clathrin assembly protein (PICALM)

  • 货号：
    CSB-YP622660HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP622660HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP622660HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP622660HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP622660HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PICALM
  • Uniprot No.：
  • 别名：
    CALM; Clathrin assembly lymphoid myeloid leukemia; Clathrin assembly lymphoid myeloid leukemia protein; CLTH; LAP; Phosphatidylinositol-binding clathrin assembly protein; PICAL_HUMAN; PICALM
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-652
  • 氨基酸序列
    SGQSLTDRI TAAQHSVTGS AVSKTVCKAT THEIMGPKKK HLDYLIQCTN EMNVNIPQLA DSLFERTTNS SWVVVFKSLI TTHHLMVYGN ERFIQYLASR NTLFNLSNFL DKSGLQGYDM STFIRRYSRY LNEKAVSYRQ VAFDFTKVKR GADGVMRTMN TEKLLKTVPI IQNQMDALLD FNVNSNELTN GVINAAFMLL FKDAIRLFAA YNEGIINLLE KYFDMKKNQC KEGLDIYKKF LTRMTRISEF LKVAEQVGID RGDIPDLSQA PSSLLDALEQ HLASLEGKKI KDSTAASRAT TLSNAVSSLA STGLSLTKVD EREKQAALEE EQARLKALKE QRLKELAKKP HTSLTTAASP VSTSAGGIMT APAIDIFSTP SSSNSTSKLP NDLLDLQQPT FHPSVHPMST ASQVASTWGD PFSATVDAVD DAIPSLNPFL TKSSGDVHLS ISSDVSTFTT RTPTHEMFVG FTPSPVAQPH PSAGLNVDFE SVFGNKSTNV IVDSGGFDEL GGLLKPTVAS QNQNLPVAKL PPSKLVSDDL DSSLANLVGN LGIGNGTTKN DVNWSQPGEK KLTGGSNWQP KVAPTTAWNA ATMAPPVMAY PATTPTGMIG YGIPPQMGSV PVMTQPTLIY SQPVMRPPNP FGPVSGAQIQ FM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors. Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF).
  • 基因功能参考文献：
    1. IN PICALM rs3851179 polymorphism, the age- dependent increase in the P3 latency was more pronounced in the PICALM GG carriers than in the carriers of the PICALM AA and PICALM AG genotypes. PMID: 30472946
    2. There were significant differences in genotype and allele frequencies for the SNP PICALM rs3851179 between Brazilian Alzheimer and Parkinson disease cases and controls, but none for CR1 rs6656401 and CLU rs11136000 intronic polymorphisms. PMID: 28567584
    3. The PICALM rs3851179 polymorphism significantly affects the default mode network network in mild cognitive impairment PMID: 28549650
    4. No association for PICALM with Alzheimer's disease in south-Indian population. PMID: 28558900
    5. this updated meta-analysis highlights the involvement of PICALM rs3851179 variant in Alzheimer's disease susceptibility in Chinese population. PMID: 27048444
    6. suggest that there is a close relationship between abnormal PICALM processing, tau pathology and impairment of autophagy in human neurodegenerative diseases PMID: 27260836
    7. There was no significant association between single nucleotide polymorphisms (SNPS) of GAB2 rs2373115 (G > T) and PICALM rs541458 (C > T) and Alzheimer's disease (AD). The allele T of rs3851179 in PICALM was associated with a 13 % increase in the risk of AD. Seven SNPs on SORL1 were significantly associated with AD. PMID: 26611835
    8. Hippocampal shape features derived from the diffeomorphic metric-based shape analysis led to the identification of significant CLU-PICALM interaction effects on hippocampal morphology in young healthy adults, which were not identified by volume measurement and voxel-wise analysis. PMID: 27017968
    9. This study provided preliminary evidences supporting that PICALM variations render protections by facilitating reserve capacities of posterior cingulate in non-demented elderly. PMID: 27117083
    10. rs3851179 in PICALM was associated with an increased risk of gestational diabetes. The frequency of the Alzheimer's disease risk-associated C allele was significantly higher in the gestational diabetes group compared to controls. The C allele of the PICALM SNP was protective for impaired glucose tolerance. PMID: 28316001
    11. Alzheimer's disease risk PICALM GG genotype suggests changes in the cortical excitatory-inhibitory balance, which are heightened during normal aging. PMID: 28073596
    12. Study concludes that the novel clathrin interaction sites identified here in CALM and AP180 have a major role in how these proteins interface with clathrin. This work advances the case that AP180 and CALM are required to use a combination of standard clathrin N-terminal domain binding motifs and the sequence identified here for optimal binding and assembling clathrin. PMID: 27574975
    13. Depletion of PICALM in brain-derived cells has significant effects on the processing of APP, probably by reducing clathrin-mediated endocytosis. In particular, it affects the production of beta-C-terminal fragment which is increasingly considered to be an important mediator in Alzheimer's disease independent of Abeta. PMID: 27430330
    14. reduction of CALM decreases Ab deposition as well as brain levels of insoluble Ab42 in vivo These results suggest that CALM expression modifies Alzheimer disease (AD)risk by regulating Aamyloid-beta protein pathology PMID: 27466196
    15. PICALM is involved with late onset Alzheimer's disease. [review] PMID: 27773727
    16. Our finding suggests that PICALM rs3851179 could contribute to cognitive impairment in older patients with Parkinson disease PMID: 26889634
    17. PICALM and CLU risk genotypes exert differential impacts on the hippocampal resting-state functional connectivity in healthy young subjects. PMID: 24578178
    18. PICALM has a role in modulating autophagy activity and tau accumulation PMID: 25241929
    19. the rs541458 and rs3851179 of PICALM SNPs are not related to development of behavioral and psychological symptoms of dementia in Turkish population PMID: 26434199
    20. We detected a significant association of the MTHFR rs1801133 and PICALM rs3851179 polymorphisms with AD. PMID: 25359311
    21. PICALM SNP is significantly associated with late onset Alzheimer disease. PMID: 25022885
    22. CALM is a major factor in controlling endocytic clathrin-coated vesicle size and maturation and hence in determining the rates of endocytic cargo uptake. PMID: 25898166
    23. Data suggest a neural mechanism for APOE-PICALM interactions in patients with manifest Alzheimer's disease and indicate that the PICALM genotype modulates both brain atrophy and cognitive performance in APOE epsilon4 carrier PMID: 24613704
    24. multiple PICALM isoforms are expressed in the human brain, that PICALM is robustly expressed in microvessels, and that expression of total PICALM is modestly correlated with the AD-associated SNP rs3851179. PMID: 24618820
    25. allelic expression has shown that compelling PICALM allelic expression imbalance was not observed in most brain RNA samples PMID: 25169757
    26. physical activity attenuated the effects of genetic risk (ie. the constellation of PICALM, BIN1, and CLU polymorphisms) on episodic memory PMID: 24660791
    27. It might not play a major role in the genetic predisposition to Late-onset Alzheimer's disease in Han Chinese. PMID: 24095218
    28. PICALM affects age at onset of Alzheimer's dementia in Down syndrome. PMID: 23601808
    29. results confirm the association of PICALM gene with Alzheimer disease susceptibility in Korean population PMID: 22975751
    30. We identified a mutation in CALM1 underlying IVF manifesting in childhood and adolescence. The causality of the mutation is supported by previous studies demonstrating that F90 mediates the direct interaction of CaM with target peptides. PMID: 24076290
    31. This results suggest that besides APOE, PICALM associated with Alzheimer's disease risk do not exert large effects on the age-at-onset phenotype of Alzheimer disease. PMID: 23870418
    32. CALM is able to sort VAMP4 and VAMP7, even though they have sorting signals for other clathrin adaptors. PMID: 23741335
    33. The PICALM rs3851179 polymorphism is significantly associated with AD in the Asian population by both pooled analysis and meta-analysis methods. PMID: 23572399
    34. AP-2/PICALM complex functions as an autophagic cargo receptor for the recognition and shipment of APP-CTF from the endocytic pathway to the LC3-marked autophagic degradation pathway. PMID: 24067654
    35. Genome-wide association studies found 3 new susceptibility loci for Alzheimer disease(AD): complement receptor 1,clusterin,and the phosphatidylinositol-binding clathrin assembly protein. These SNPS are not associated with AD in a Polish population. PMID: 23650005
    36. A CALM-derived nuclear export signal is essential for CALM-AF10-mediated leukemogenesis. PMID: 23487024
    37. Our results indicate that rs3851179 may not be an AD susceptibility locus in the Chinese population and the APOEepsilon4-negative subgroup. PMID: 23040034
    38. model system to study the effects of intracellular Abeta in fusion with green fluorescent protein. We sent this fusion protein into the secretory pathway and showed that intracellular traffic pathways are necessary for the generation of toxic species. PMID: 22888099
    39. rs592297, a known coding synonymous SNP that is part of an exonic splice enhancer region in exon 5 of the PICALM gene, is in strong linkage disequilibrium with rs3851179. PMID: 22943764
    40. Our results do not provide evidence that the PICALM rs3851179 polymorphism increase susceptibility to Parkinson disease, in the Greek population. PMID: 22715855
    41. AP180 and CALM are endocytic adaptors dedicated to the sorting of small soluble N-ethylmaleimide-sensitive-factor attachment protein receptors. (Review) PMID: 22639918
    42. CLU and CR1 were associated with more rapid cognitive decline. PICALM was associated with an earlier age at midpoint of cognitive decline. PMID: 22952074
    43. In conclusion, we confirmed association of CLU, CR1, and PICALM genes with the disease status in our cohort through identification of a number of disease-specific variants PMID: 22402018
    44. While no Alzheimer Disease association was observed with single nucleotide polymorphisms, a trend of association was seen with the Picalm and Clusterin single nucleotide polymorphisms. PMID: 20570404
    45. PICALM, an adaptor protein involved in clathrin-mediated endocytosis, regulates APP internalization and subsequent Abeta generation. PICALM contributes to amyloid plaque load in brain likely via its effect on Abeta metabolism. PMID: 22539346
    46. The SNP genotype pattern at the PICALM gene is associated with episodic memory. PMID: 22539578
    47. Single nucleotide polymorphisms (SNPs) are not significantly different within PICALM sporadic Alzheimer disease patients and unrelated age- and sex-matched healthy controls of Han Chinese. PMID: 21358043
    48. variants in BIN1, CLU, CR1 or PICALM are associated with changes in the CSF levels of biomarkers PMID: 21347408
    49. findings showed evidence of CR1, CLU, and PICALM and late-onset Alzheimer's disease (LOAD) susceptibility in an independent southern Chinese population PMID: 22015308
    50. AP-2 and epsin-1 are both required to promote efficient internalization of activated PAR1 and recognize discrete receptor sorting signals PMID: 21965661

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  • 相关疾病：
    A chromosomal aberration involving PICALM is found in diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with MLLT10.
  • 亚细胞定位：
    Cell membrane. Membrane, clathrin-coated pit. Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle. Nucleus.
  • 蛋白家族：
    PICALM/SNAP91 family
  • 组织特异性：
    Expressed in all tissues examined.
  • 数据库链接：

    HGNC: 15514

    OMIM: 603025

    KEGG: hsa:8301

    STRING: 9606.ENSP00000377015

    UniGene: Hs.163893