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  4. Recombinant Human Phorbol-12-myristate-13-acetate-induced protein 1 (PMAIP1)

Recombinant Human Phorbol-12-myristate-13-acetate-induced protein 1 (PMAIP1)

  • 货号：
    CSB-YP018229HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018229HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP018229HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018229HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018229HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Adult T cell leukemia derived PMA responsive; APR; APR_HUMAN; ATL-derived; Immediate early response protein APR; Immediate-early-response protein APR; NOXA; Phorbol 12 myristate 13 acetate induced protein 1; Phorbol-12-myristate-13-acetate-induced protein 1; PMA induced protein 1; PMA-induced protein 1; PMA-responsive gene; Pmaip1; Protein Noxa
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-136
  • 氨基酸序列
    MPGKKARKNAQPSPARAPAGPAGTAGTARDQAGFAIGMQLRFTRGKKLLSSSLSSSPLAL PRGHEEQVQVAGSRVCYSTQEIWRQTELPAETSESDIQTLLLRNLTASKTCMRGLLQKSF LRRCTFHQFEERLHCN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1. Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1.
  • 基因功能参考文献：
    1. Data report that NOXA is a physiological substrate of autophagy. Mechanistic studies revealed that NOXA is hijacked by p62 for degradation during the autophagic process, and the three lysine residues at the C-terminus of NOXA are required for its autophagic degradation. Furthermore, autophagy can block apoptosis by eliminating NOXA in both p53-dependent and p53-independent manners. PMID: 29758299
    2. Fluorizoline increased the protein levels of the pro-apoptotic B-cell lymphoma 2 family member NOXA in chronic lymphocytic leukemia cells. Furthermore, fluorizoline synergized with ibrutinib, 5-aminoimidazole-4-carboxamide riboside or venetoclax to induce apoptosis. PMID: 28619845
    3. By ubiquitylating and degrading NOXA through activating CRL5, UBE2F selectively promotes lung cancer cell survival and could, therefore, serve as a novel cancer target PMID: 27591266
    4. Data show that Noxa-mediated MCL-1 phosphorylation and degradation is regulated by CDK2. PMID: 27166195
    5. the c-FLIP and NOXA/Mcl-1 axis participated in the synergistic effect of pemetrexed plus cisplatin in human choroidal melanoma cells PMID: 28863158
    6. Noxa is involved in X-ray-induced lung injury. PMID: 27862899
    7. Arsenic trioxide induces Noxa-dependent apoptosis in rhabdomyosarcoma cells and acts synergistically with antimicrotubule drugs. PMID: 27521572
    8. knockdown of MCL-1 in MRT cell lines induced apoptosis and increased DOX sensitivity in malignant rhabdoid tumor cells PMID: 26680268
    9. high levels of miR21 expression may induce gastric cancer migration and invasion via the downregulation of Noxa expression PMID: 26847916
    10. Inhibition of SIRT1 could induce the Noxa expression in A549/DDP cells. PMID: 26903157
    11. Fluorizoline bind to prohibitin, inducing mitochondrial apoptotic pathway through NOXA and BIM upregulation. PMID: 26497683
    12. Noxa demethylation has a role in Bortezomib resistance in mantle cell lymphoma PMID: 25714012
    13. SATB1 as a Dual Regulator of Anti-Apoptotic BCL2 and Pro-Apoptotic NOXA Genes PMID: 26422397
    14. ALK-negative anaplastic large cell lymphoma is sensitive to bortezomib through Noxa upregulation and release of Bax from Bcl-2. PMID: 25975837
    15. BCL2 and BCLX phosphorylation represents a priming event in mitotic cell death that is triggered by NOXA-dependent MCL1 degradation. The MCL1 decay allows in turn BIM-dependent cell death. PMID: 25922916
    16. Overexpression of nonphosphorylatable Noxa resulted in enhanced mitochondria-mediated apoptosis in the infected epithelium. PMID: 25404713
    17. Results reveal that oncogenic activation of MEK/ERK drives Noxa expression to promote autophagy, and suggest that Noxa has an indirect anti-apoptosis role in melanoma cells under nutrient starvation conditions. PMID: 25365078
    18. Overall, these data reveal a Noxa-mediated signaling pathway that couples lysosomal membrane permeabilization with mitochondrial outer membrane permeabilization and ultimate apoptosis during oxidative stress. PMID: 23770082
    19. CDK5 activity provides resistance to heat-induced apoptosis through the expression of miR-23a and subsequent suppression of NOXA synthesis PMID: 25829494
    20. Mcl-1/Noxa axis is important to overcome resistance to mitochondrial apoptosis in squamous cell carcinoma. PMID: 25210795
    21. Cells overexpressing HSP70 had higher levels of miR-23a, maintained these levels after heat shock and accumulated lower levels of NOXA mRNA and protein. PMID: 25429623
    22. Noxa upregulation reduces the availability of Usp9x to Mcl-1, thereby promoting its ubiquitination and degradation, leading to the apoptosis of neoplastic cells. PMID: 24991768
    23. Overexpression of ApoL2 did not induce cell death on its own. ApoL2 did not sensitize or protect cells from overexpression of the BH3-only proteins Bmf or Noxa. PMID: 24901046
    24. Noxa recruits MCL-1 from the cytosol to the mitochondria modulating ABT-737 sensitivity in small cell lung cancer. PMID: 24525728
    25. these results suggest that the C-terminal tail of Noxa regulates the stability of both Noxa and Mcl-1. PMID: 24811167
    26. Mantle cell lymphoma are characterized by high constitutive NOXA gene expression but low levels of NOXA protein. PMID: 24457957
    27. Parthenolide triggers extrinsic apoptosis by up-regulating TNFRSF10B and intrinsic apoptosis through increasing the expression of PMAIP1. PMID: 24387758
    28. Knockdown of TRAIL and Noxa reduced FOXO3-induced apoptosis, implicating that mitochondrial destabilization amplifies TRAIL-signaling. PMID: 23828551
    29. Physalin A-induced apoptosis was triggered by activation of p53-Noxa pathway. PMID: 23684722
    30. CXCR4 chemokine receptor signaling induces apoptosis in acute myeloid leukemia cells via regulation of the Bcl-2 family members Bcl-XL, Noxa, and Bak. PMID: 23798675
    31. caspases contribute to trigger the mitochondrial apoptotic pathway by coupling Bcl-2/Bcl-xL inhibition to that of Mcl-1, via the pRb/E2F-1-dependent induction of Noxa PMID: 23429261
    32. Vorinostat, is able to ablate the multicellular resistance to bortezomib by its ability to restore expression of a key pro-apoptotic Bcl-2 family member, Noxa. PMID: 23300762
    33. NOXA, a sensor of proteasome integrity, is degraded by 26S proteasomes by an ubiquitin-independent pathway that is blocked by MCL-1. PMID: 22361683
    34. Noxa is a central determinant of hypersensitivity to cisplatin PMID: 23302226
    35. Oncolytic viruses selectively induced expression of TRAIL and Noxa in prostate cancer cells, lung cancer cells, and breast cancer cells downstream of RIG-I and MAVS. PMID: 23014529
    36. Novel but opposing roles for the p53 and TAp73 in the induction of Noxa and Bim and regulation of apoptosis. PMID: 22430213
    37. Our results suggested that polymorphisms of NOXA and MCL1 may modify the risk of HPV16-associated oropharyngeal cancer. PMID: 22548841
    38. detailed analysis of the Noxa promoter revealed that p73 and Sp1-like factors, Sp1 and KLF6, played key roles in the transcriptional control of this gene PMID: 22718761
    39. these data suggest a new mechanism by which NOXA chemosensitized ovarian cancer cells to cisplatin by inducing alterations in the Bax/Smac axis. PMID: 22590594
    40. show that miR-200c is a novel negative regulator of the pro-apoptotic Bcl-2 family member Noxa PMID: 22615771
    41. during skin hypoxia, promotes UVB-induced apoptosis in keratinocytes PMID: 22245094
    42. siRNA-mediated knockdown of Noxa resulted in cell cycle arrest following E2 treatment, indicating that Noxa expression is required for cell cycle progression in ER-positive breast cancer cells. PMID: 22216287
    43. these study suggested that the four mutations lowered the stability of the p53 DBD domain and induced aggregation of structurally destabilized p53, and thus disrupted the p53-PUMA/NOXA interaction. PMID: 22446329
    44. Multiple BH3 mimetics antagonize antiapoptotic MCL1 protein by inducing the endoplasmic reticulum stress response and up-regulating BH3-only protein NOXA. PMID: 21628457
    45. Bortezomib can enhance sensitivity of NB4 cells to apoptosis induced by As(2)O(3) which may be related to up-regulation of proapoptotic protein NOXA. PMID: 21518486
    46. Noxa is critical for sensitivity to cell death induced by 2-deoxyglucose. This is the first evidence of the implication of a BH3-only protein in death induced by 2-DG. PMID: 21911456
    47. Noxa triggers the degradation of Mcl-1 at the mitochondria according to the exclusive location of Noxa at this compartment. PMID: 21907705
    48. Noxa/Mcl-1 balance is regulated by glucose deprivation as well as by general metabolic stress PMID: 21516346
    49. A pathway leading from the detection of viral RNA during modified vaccinia virus infection by the cytosolic helicase-pathway, to the up-regulation of Noxa and apoptosis via IRF3 and type I IFN signalling. PMID: 21698224
    50. Noxa participates in triggering mitochondrial dysfunction in multiple apoptotic pathways through distinct mechanisms PMID: 21113147

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  • 亚细胞定位：
    Mitochondrion.
  • 蛋白家族：
    PMAIP1 family
  • 组织特异性：
    Highly expressed in adult T-cell leukemia cell line.
  • 数据库链接：

    HGNC: 9108

    OMIM: 604959

    KEGG: hsa:5366

    STRING: 9606.ENSP00000326119

    UniGene: Hs.96