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  4. Recombinant Human Perilipin-2 (PLIN2)

Recombinant Human Perilipin-2 (PLIN2)

  • 货号：
    CSB-YP860767HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP860767HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP860767HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP860767HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP860767HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    ADFP; Adipophilin; Adipose differentiation related protein; Adipose differentiation-related protein; ADRP; MGC10598; Perilipin-2; PLIN2; PLIN2_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-437
  • 氨基酸序列
    ASVAVDPQP SVVTRVVNLP LVSSTYDLMS SAYLSTKDQY PYLKSVCEMA ENGVKTITSV AMTSALPIIQ KLEPQIAVAN TYACKGLDRI EERLPILNQP STQIVANAKG AVTGAKDAVT TTVTGAKDSV ASTITGVMDK TKGAVTGSVE KTKSVVSGSI NTVLGSRMMQ LVSSGVENAL TKSELLVEQY LPLTEEELEK EAKKVEGFDL VQKPSYYVRL GSLSTKLHSR AYQQALSRVK EAKQKSQQTI SQLHSTVHLI EFARKNVYSA NQKIQDAQDK LYLSWVEWKR SIGYDDTDES HCAEHIESRT LAIARNLTQQ LQTTCHTLLS NIQGVPQNIQ DQAKHMGVMA GDIYSVFRNA ASFKEVSDSL LTSSKGQLQK MKESLDDVMD YLVNNTPLNW LVGPFYPQLT ESQNAQDQGA EMDKSSQETQ RSEHKTH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：
    Perilipin-2（PLIN2）又称脂肪分化相关蛋白（ADRP），是属于危险脂蛋白家族的一种蛋白质。它主要在脂肪细胞中表达，并作为一个与脂滴相关的蛋白质，调节三酰甘油的储存和水解。
     
    重组人危险脂蛋白-2（PLIN2）是通过使用编码PLIN2蛋白质的人类DNA序列进行基因工程技术生产的蛋白质。它可用于各种研究应用，以研究PLIN2在脂肪细胞生物学、脂质代谢和与肥胖相关的疾病中的作用。
     
    重组PLIN2蛋白可以在细菌、酵母或哺乳动物表达系统中生产，并使用各种色谱技术进行纯化。然后，纯化的蛋白质可以用于体外实验，以研究其功能，例如其与脂滴的结合能力或调节脂质代谢的能力。
     
    总的来说，重组人PLIN2是了解这种重要蛋白质的生物学功能及其在代谢性疾病发展中潜在作用的有价值工具。
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May be involved in development and maintenance of adipose tissue.
  • 基因功能参考文献：
    1. Study utilizing THP-1 macrophages concludes that long-chain metabolite of vitamin E, alpha-13'-carboxychromanol, protects cells from lipotoxicity, at least partially via PLIN2 regulation. PMID: 29733984
    2. PLIN2 protein levels are significantly increased in patients with neoatherosclerosis. PMID: 29422264
    3. hese results suggested that PLIN2 may be considered a novel prognostic factor in Clear cell renal cell carcinoma (ccRCC) and a specific diagnostic indicator for patients with ccRCC. Furthermore, it could be a potential novel target for the clinical treatment of ccRCC PMID: 29749470
    4. ADRP knockdown significantly inhibited PDGFinduced increases in Vascular smooth muscle cell viability, caused G1 phase cell cycle arrest and decreased PCNA expression. PMID: 28713961
    5. this papet shows that adipophilin is a novel prognostic biomarker in clear cell renal cell carcinoma PMID: 28404922
    6. PLIN2 gene Ser251Pro missense mutation is associated with reduced insulin secretion and increased insulin sensitivity. PMID: 26443937
    7. Interference with PLIN2 and PPARalpha resulted in major alterations in gene expression, especially affecting lipid, glucose, and purine metabolism. PMID: 27308945
    8. This study shows that hepatitis C virus suppresses hepatic ADRP expression in infected patients and cell lines. Forcing the expression of miR-148a and miR-30a limits the suppressive effect of hepatitis C virus on ADRP. PMID: 27591428
    9. ADP was expressed in a small proportion of lung adenocarcinoma suggesting that ADP-positive lung adenocarcinoma could be a distinct subtype of lung adenocarcinoma, induced by up-regulation of the lipogenic pathway. PMID: 27467545
    10. Activation of ARF1 dissociates ADRP from lipid droplets. A constitute active form of ARF1 (ARF1Q71I) promotes HCV assembly. ADRP played a positive role in Hepatitis C virus replication and negative role in Hepatitis C virus assembly. PMID: 27157138
    11. PLIN2 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
    12. Adipophilin was present in 24 of 26 colorectal hyperplastic polyps, but did not correlate with presence of white opaque substance (i.e. lipid droplets) on magnifying endoscopy with narrow band imaging. PMID: 26636961
    13. Conserved amphipathic helices mediate lipid droplet targeting of PLIN1, PLIN2, and PLIN3. PMID: 26742848
    14. In hepatitis C virus -infected human livers, occludin and ADRP mRNA expression levels correlated with each other. Hepatitis C virus increases occludin expression via the upregulation of ADRP. PMID: 26731658
    15. PLIN2 involves lipid modulation of GSK3 activity, GSK3 substrate expression, and cell growth/survival. PMID: 26598603
    16. PLIN2 inhibits insulininduced glucose uptake by activating NLRP3, caspase1 and IL1beta, leading to a decreased IRS1 expression. PMID: 26166692
    17. Plin2 appears to be associated with functional alterations of the muscle PMID: 25559404
    18. Results demonstrate the clinical utility, specificity, and sensitivity of urine AQP1 and PLIN2 to diagnose renal cell carcinoma. PMID: 26181025
    19. Postprandial triglyceride rich lipoproteins may be involved in atherosclerotic plaque formation through the regulation of perilipin-2 and perilipin-3 proteins in macrophages. PMID: 25595097
    20. Immunohistochemical distinction of renal cell carcinoma from other carcinomas with clear-cell histomorphology: utility of CD10 and CA-125 in addition to PAX-2, PAX-8, RCCma, and adipophilin. PMID: 25279712
    21. ADRP was detectable in human BM during the whole 12 months of lactation period and its levels were intraindividually well-conserved. PMID: 24577896
    22. Perilipin 2 binding to the lipid droplet surface requires both hydrophobic and electrostatic interactions. PMID: 25172666
    23. Fat deposition within skeletal muscle in form of Plin2-coated lipid droplets increases with age and is associated with decreased muscle strength and thickness, likely through an IGF-1- and p53-dependent mechanism. PMID: 23977392
    24. Mammary analogue secretory carcinoma is a lipid-rich tumour containing large lipid droplets covered by adipophilin. PMID: 23931576
    25. Overexpression of ADRP alone increased the formation of foam cells and consequently autophagic activity. PMID: 24405744
    26. Data indicate that the allele of the missense polymorphism Ser251Pro disrupts a predicted alpha-helix in perilipin 2 (PLIN2). PMID: 23603836
    27. PLIN2 is the major perilipin regulated during sebocyte differentiation in vitro. PLIN2 is also important for sebaceous lipid accumulation in vitro and regulates sebaceous gland size in vivo PMID: 23688400
    28. Sprint interval and traditional endurance training increase net intramuscular triglyceride breakdown and expression of perilipin 2 and 5 PMID: 23129790
    29. Gene expression profile indicated a significant over-expression of the adipophilin gene and marked up-regulation of other genes involved in lipid metabolism in Burkitt lymphoma. PMID: 22952953
    30. Data suggest that prolonged endurance exercise training increases expression of perilipin 2 alongside increases in intramuscular triglyceride content specificly in type I/slow-twitch muscle fibers of type 2 diabetes patients. PMID: 22949030
    31. It was shown that the PLIN2 content in skeletal muscle is unchanged in response to a single bout of endurance exercise. The PLIN2-intramuscular triglyceride association was reduced postexercise. PMID: 22496505
    32. PLIN2-PLIN5 proteins were all more abundant in women than in men (p = 0.037 and p < 0.0001, respectively), consistent with higher intramyocellular lipid content observed in female skeletal muscle. PMID: 22667335
    33. High PLIN2 protein level is associated with colorectal cancer. PMID: 21828233
    34. The frequency of adipophilin-positive cases was significantly higher in apocrine breast carcinomas compared with nonapocrine carcinomas. PMID: 21566511
    35. Adipophilin-positive macrophage infiltration in the fibrous cap might be correlated with instability in neurological status PMID: 20887090
    36. Perilipin may play a role in the long-term storage of fat, which may be only partially reflected by cell culture models. PMID: 20661576
    37. Adipophilin can be valuable in an immunohistochemical panel when evaluating cutaneous lesions with clear cell histology PMID: 20118912
    38. Adipophilin augment inflammation in macrophages, which might be one role of adipophilin in atherosclerosis. PMID: 19851831
    39. ADRP upregulation mediated by retinol and palmitate promotes downregulation of hepatic stellate cells activation and is functionally linked to the expression of fibrogenic genes. PMID: 20143336
    40. Expression patterns of perilipin and adipophilin in nonalcoholic fatty liver disease livers vary with the size of lipid droplets PMID: 20032580
    41. Findings revealed an upregulation of ADRP during macrophages differentiation into foam cells. PMID: 19351497
    42. LXR, upon stimulation with GW3965, directly regulates human ADFP transcription by binding to LXR response elements located in the 3'-untranslated and the 5'-flanking regions PMID: 19843633
    43. induction by hypoxia PMID: 12387890
    44. targeting of the lipid droplet-binding adipocyte differentiation-related protein. PMID: 12591929
    45. Expression of adipophilin is enhanced during trophoblast differentiation and is up-regulated by ligand-activated PPARgamma/retinoid X receptor. May contribute to fatty acid uptake by placenta. PMID: 14671211
    46. Stimulation of adipophilin expression in macrophages by modified LDL promotes triglyceride & cholesterol storage and reduces cholesterol efflux. Adipophilin might contribute in vivo to lipid accumulation in the intima of the arterial wall. PMID: 14707038
    47. GDP-bound ARF1 induces dissociation of ADRP from the Lipid droplet surface;. PMID: 15336557
    48. TIP47 and adipophilin are components of many, but not all, the lipid droplets in THP-1 cells PMID: 15545278
    49. ADRP and TG regulate each other, and the ubiquitin-proteasome system is involved in degradation of ADRP during regression of lipid-storing cells. PMID: 16230742
    50. Data demonstrate that adipose differentiation-related protein (ADRP) mRNA and protein are regulated by fatty acids in a human placental choriocarcinoma cell line (BeWo) and in primary human trophoblasts. PMID: 16391323

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  • 亚细胞定位：
    Membrane; Peripheral membrane protein. Lipid droplet.
  • 蛋白家族：
    Perilipin family
  • 组织特异性：
    Milk lipid globules.
  • 数据库链接：

    HGNC: 248

    OMIM: 103195

    KEGG: hsa:123

    STRING: 9606.ENSP00000276914

    UniGene: Hs.3416