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  4. Recombinant Human Partitioning defective 6 homolog alpha (PARD6A)

Recombinant Human Partitioning defective 6 homolog alpha (PARD6A)

  • 货号：
    CSB-YP878839HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP878839HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP878839HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP878839HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP878839HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    0710008C04Rik; 2610010A15Rik; Par 6 partitioning defective 6 C elegans homolog alpha; Par 6 partitioning defective 6 homolog alpha; Par 6 partitioning defective 6 homolog alpha C elegans; PAR 6A; PAR-6 alpha; PAR-6; par-6 family cell polarity regulator alpha; PAR-6A; PAR6; PAR6A_HUMAN; PAR6alpha; PAR6C; Pard6a; Partitioning defective 6 homolog alpha; partitioning-defective protein 6; partitioning-defective protein 6, C. elegans, homolog of, alpha; Tax interacting protein 40; Tax interaction protein 40; TAX40; TIP 40; TIP-40; TIP40
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-346
  • 氨基酸序列
    MARPQRTPAR SPDSIVEVKS KFDAEFRRFA LPRASVSGFQ EFSRLLRAVH QIPGLDVLLG YTDAHGDLLP LTNDDSLHRA LASGPPPLRL LVQKRAEADS SGLAFASNSL QRRKKGLLLR PVAPLRTRPP LLISLPQDFR QVSSVIDVDL LPETHRRVRL HKHGSDRPLG FYIRDGMSVR VAPQGLERVP GIFISRLVRG GLAESTGLLA VSDEILEVNG IEVAGKTLDQ VTDMMVANSH NLIVTVKPAN QRNNVVRGAS GRLTGPPSAG PGPAEPDSDD DSSDLVIENR QPPSSNGLSQ GPPCWDLHPG CRHPGTRSSL PSLDDQEQAS SGWGSRIRGD GSGFSL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Regulates centrosome organization and function. Essential for the centrosomal recruitment of key proteins that control centrosomal microtubule organization.
  • 基因功能参考文献：
    1. In T84 cells, overexpression of Par-6 causes intestinal barrier dysfunction. Lipopolysaccharide (LPS)-induced intestinal epithelial barrier dysfunction and increase in Par-6 expression was prevented by AhR activation. PMID: 29992488
    2. Studies indicate that the PAR3-PAR6-aPKC complex are important for the establishment of neuronal polarity [Review]. PMID: 29696344
    3. Roles of partitioning-defective protein 6 (Par6) and its complexes in the proliferation, migration and invasion of cancer cells PMID: 28590507
    4. Shp2 promotes metastasis of prostate cancer by attenuating the PAR3/PAR6/aPKC polarity protein complex and enhancing epithelial-to-mesenchymal transition PMID: 26050620
    5. TGFbeta induced Par6 phosphorylation on Ser345 and its recruitment to the leading edge of the membrane ruffle in migrating PC-3U cells, where it colocalised with aPKCzeta. The p-Par6-aPKCzeta complex is important for cell migration and invasion. PMID: 25756394
    6. Data indicate that both tumor focality and Par3/Par6/atypical protein kinase C (APKC) expression were significantly associated with tumor recurrence. PMID: 21549621
    7. BDNF can regulate formation of functional synapses by increasing the expression of the RhoA inhibitors, Par6C and Rnd3. PMID: 23762244
    8. Morg1 facilitates Par6-aPKC binding to Crb3 for definition of apical identity of epithelial cells. PMID: 23439680
    9. Par6 negatively regulates trophoblast fusion via its roles on tight junctions and cytoskeleton dynamics and provide novel insight into the contribution of this polarity marker in altered trophoblast cell fusion typical of preeclampsia. PMID: 23341197
    10. Atypical protein kinase C kinase activity, as well as an association with PAR6, were found to be important for PAR6 phosphorylation. PMID: 23249950
    11. Pak6 is a binding partner and a outatuve effector protein for the atypical rho GTPase cdc42 homologous protein. PMID: 22339630
    12. Data show that DDR1 coordinates the Par3/Par6 cell-polarity complex through its carboxy terminus, binding PDZ domains in Par3 and Par6. PMID: 21170030
    13. Par6alpha controls centrosome organization through its association with the dynactin subunit p150(Glued). PMID: 20719959
    14. Partitioning-defective protein 6 associated constitutively with endogenous aPKCs. PMID: 14976222
    15. crystal structure of the complex of PKCiota and Par6alpha PB1 domains to a resolution of 1.5 A PMID: 15590654
    16. A rare A/G polymorphism in the promoter of the Par6alpha gene is associated with reduced fasting glycaemia, increased glucose tolerance and reduced serum nonesterified fatty Acids concentrations. PMID: 15744531
    17. findings demonstrated that G-protein-activated phospholipase C-beta interacts with cell polarity proteins Par3 and Par6 to form protein complexes and to mediate downstream signal transduction PMID: 15782111
    18. Studies in this review confirm that signaling by Par6alpha controls the migration of immature granule neurons down the Bergmann glial fibers into the internal granule cell layer in which they establish synaptic connections. PMID: 17050699
    19. We provide evidence for the existence of a distinct PAR protein complex in endothelial cells. Both PAR-3 and PAR-6 associate directly with the adherens junction protein vascular endothelial cadherin (VE-cadherin). PMID: 17057644
    20. Par6alpha-mediated inhibition of insulin-dependent glycogen synthesis in C2C12 cells depends on the direct interaction of Par6alpha with aPKC and on aPKC-mediated T34 phosphorylation of Akt1. PMID: 17335965
    21. Par6 was characterized as a dual-location protein. PMID: 17420281
    22. Shear stress-induced directed migratory polarity is modulated by exogenous growth factors and dependent on Par6 activity and shear stress direction in endothelial cells PMID: 17586613
    23. In vivo binding studies identified a novel mechanism of Par6 interaction, suggesting that the cell polarity machinery may serve to spatially restrict Rit signaling. PMID: 17976838
    24. Neph1-Nephrin proteins bind the Par3-Par6-atypical protein kinase C (aPKC) complex to regulate podocyte cell polarity PMID: 18562307
    25. in addition to regulating cell polarization processes, Par6 is an inducer of cell proliferation in breast epithelial cells. PMID: 18922891
    26. Ect2 and PKCiota are genetically and functionally linked in NSCLC, acting to coordinately drive tumor cell proliferation and invasion through formation of an oncogenic PKCiota-Par6alpha-Ect2 complex. PMID: 19617897
    27. the TGFbeta-Par6 polarity pathway has a role in breast cancer progression PMID: 19667198

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  • 亚细胞定位：
    Cytoplasm. Cell membrane. Cell projection, ruffle. Cell junction, tight junction. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 蛋白家族：
    PAR6 family
  • 组织特异性：
    Expressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta.
  • 数据库链接：

    HGNC: 15943

    OMIM: 607484

    KEGG: hsa:50855

    STRING: 9606.ENSP00000219255

    UniGene: Hs.112933